Evaluating the correlation between pretreatment 18F-FDG PET/CT metabolic parameters and tumor-infiltrating lymphocyte levels in nonluminal breast cancer and impact on survival by Muge Tamam, Halim Ozcevik, Gamze Kulduk, Merve Nur Acar Tayyar, Gunduzalp Bugrahan Babacan

“…TIL was scored based on Hematoxylin-Eosin-stained specimens and 18F-FDG PET metabolic parameters, including maximum standardized uptake value (SUVmax), mean standardized uptake value (SUVmean), metabolic tumor volume (MTV), total lesion glycolysis (TLG), liver, spleen, and bone marrow FDG uptake were calculated. …”
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Lentinan Attenuates Damage of the Small Intestinal Mucosa, Liver, and Lung in Mice with Gut-Origin Sepsis by Zhongshen Kuang, Tingting Jin, ChangYi Wu, Yanan Zong, Panpan Yin, WangYu Dong, Xue Lin, WeiYan You, Chenlong Zhang, Lijie Wang, Yongying Liu, Shan Ren, Jiangwen Yin, Hang Xu

“…The mice were randomly divided into six groups: sham operation group (sham), gut-origin sepsis model group (CLP), ulinastatin-positive drug control group (UTI), lentinan low concentration group (LTN-L, 5 mg/kg), lentinan medium concentration group (LTN-M, 10 mg/kg), and lentinan high concentration group (LTN-H, 20 mg/kg). H&E staining was used to detect the pathological damage of the small intestine, liver, and lung. …”
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Effects of Neural Stem Cells combine with Nerve Growth Factor Nanoparticles Transplantation in Alzheimer's Disease Transgenic Mice by Qing ZHU, Shuai SHAO, Nan HU, Yan CHEN

“…Western blotting was used to detect ChAT protein expression in the basal forebrain. Thioflavine-T staining was used to detect the numbers of Aβ plaques in the hippocampus and cortex.Results:①After transplantation for four weeks, EGFP-positive NSC survived in the brain, migrated extensively to corpus callosum, cortex and deep hippocampus, and differentiated into neurons and glial cells, NSC in the combined group survived more than those in the NSC transplant group and showed more complex synaptic morphology.②The number of ChAT neurons in the basal forebrain of the NSC transplant group and the combined group increased significantly(<italic>P</italic>&lt;0.05), compared with the NSC transplant group, the gene expression of ChAT and Lhx8 in the combined group were increased significantly(<italic>P</italic>&lt;0.05).③The number of Aβ plaques in hippocampus and cortex of the NSC transplant group and the combined group didn't decreased significantly(<italic>P</italic>&gt;0.05).Conclusion:NSC combined with NGF nanoparticles transplantation could indirectly protect cholinergic neurons in the basal forebrain and the survival and maturation of transplanted NSC, and promote the gene expression of ChAT and Lhx8 in the basal forebrain, but could not reduce the number of Aβ plaques in the hippocampus and cortex.…”
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Biofabrication of zinc oxide nanoparticles using Moringa oleifera, characterization and statistical optimization for their application in crystal violet adsorption by Meshayil M. Alsolmi, Noura El-Ahmady El-Naggar, Mashael I. Alqurashi, Ragaa A. Hamouda

“…It is also applied for paper printing and Gram staining. Previously, it was significant as a topical antiseptic due to its antibacterial, antifungal, and anthelmintic properties. …”
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Androgen receptor overexpression by immunohistochemistry in malignant salivary gland tumors in Tanzania by Subira Matiku, Gad Murenzi, Eulade Rugengamanzi, Edwin Liwa, Idd Shaban, Aron Orgenes, Erick P. Magorosa, Amos Mwakigonja, Edda Vuhahula

“…Methodology This was a retrospective cross-sectional analytical study of archived paraffin embedded tissue blocks of malignant SGTs diagnosed at MNH Central Pathology Laboratory (CPL) from January 2019 to December 2022. IHC staining using a monoclonal Rabbit Anti-Human AR and interpretation was done using Allred score. …”
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Synthesis and Characterization of Paclitaxel-Loaded Silver Nanoparticles: Evaluation of Cytotoxic Effects and Antimicrobial Activity by Tutku Tunç, Ceylan Hepokur, Afşin Kari̇per

“…The antiproliferative effects of the synthesized nanoparticles were determined by XTT (tetrazolium dye; 2,3-bis-(2-methoxy-4-nitro-5-sulfenyl)-(2H)-tetrazolium-5-carboxanilide), and the proapoptotic effects were determined by annexin V/propidium iodide (PI) staining. The effect of AgNPs-PTX was more effective, and anticancer activity was higher than PTX in all cell lines. …”
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Trichinella spiralis excretory/secretory proteins mediated larval invasion via inducing gut epithelial apoptosis and barrier disruption. by Qi Qi Lu, Wen Wen Zheng, Zhao Yu Zhang, Pei Kun Cong, Xin Guo, Yao Zhang, Xin Zhuo Zhang, Shao Rong Long, Ruo Dan Liu, Zhong Quan Wang, Jing Cui

“…The IIL ESP-induced Caco-2 cell apoptosis was observed by DAPI, Hoechst 33358, TUNEL and Annexin V/PI staining. Besides, flow cytometry revealed an increasing apoptosis rate in Caco-2 cells after the IIL ESP treatment. qPCR and Western blot analysis indicated that IIL ESP activated caspases (Caspase 3, Caspase 9 and Caspase 8), up-regulated the pro-apoptotic factors (Bax and Cytochrome c) and down-regulated the anti-apoptosis molecule Bcl-2. …”
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Human Umbilical Cord Mesenchymal Stem Cells Attenuate Ocular Hypertension-Induced Retinal Neuroinflammation via Toll-Like Receptor 4 Pathway by Shangli Ji, Jie Xiao, Jian Liu, Shibo Tang

“…Retinal cell apoptosis was examined by TUNEL staining and Bax expression 14 days following hUC-MSC transplantation. …”
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Maternal nutrition and offspring lung health: sex-specific pathway modulation in fibrosis, metabolism, and immunity by Shuangyi Zhao, Zhimin Chen, Huina Liu, Xinyan Wang, Xiuru Zhang, Huirong Shi

“…Wet-lab validation, including Masson trichrome staining, immunofluorescence for α-SMA, and qRT-PCR for fibrotic markers (α-SMA, collagen I, Fn1, and TGF-β), was conducted to confirm fibrosis and other injury markers. …”
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Luteolin Regulates the Differentiation of Regulatory T Cells and Activates IL-10-Dependent Macrophage Polarization against Acute Lung Injury by Ke Xie, Yu-sen Chai, Shi-hui Lin, Fang Xu, Chuan-jiang Wang

“…In this paper, we used cecal ligation puncture (CLP) to generate an ALI mouse model to determine the effects of luteolin on ALI/ARDS. Lung tissues were stained for interleukin- (IL-) 17A and myeloperoxidase (MPO) by immunohistochemical analysis. …”
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Comparative analysis of viability, proliferation, and mineralization potential of human pulp and osteoblastic cells exposed to different bioceramic endodontic sealers by Marcos Coelho Santiago, Gustavo Henrique de Oliveira Salles, Gustavo Gomes de Lima, Laudimar Alves de Oliveira, Loise Pedrosa Salles

“…To investigate the potential of mineralization, we assessed the alkaline phosphatase activity and calcium deposition by Alizarin red staining. Statistical analysis was performed using Two-way ANOVA for calcium release and wound healing assays and One-way ANOVA for other assays, with post-test Bonferroni correction. …”
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Prognostic factors for head and neck cancer of unknown primary including the impact of human papilloma virus infection by Lars Axelsson, Jan Nyman, Hedda Haugen-Cange, Mogens Bove, Leif Johansson, Shahin De Lara, Anikó Kovács, Eva Hammerlid

“…Sixty-nine percent of the tumors were HPV positive using p16 staining. Patients who were older than 70 years, patients with N3-stage tumors, and patients with tumors that were p16 negative had a significantly worse prognosis. …”
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Morphological and molecular characterization of a Sarcocystis bovifelis-like sarcocyst in American beef by Aditya Gupta, Larissa S. de Araujo, Andrew Hemphill, Asis Khan, Benjamin M. Rosenthal, Jitender P. Dubey

“…Methods Beef samples were examined for Sarcocystis infection, using acid-pepsin digestion to search for bradyzoites, microscopically by compression between a glass slide and coverslip, by histology of paraffin embedded sections stained with hematoxylin and eosin, and by transmission electron microscopy (TEM). …”
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Paeoniflorin alleviates sepsis-associated acute kidney injury in mice by inhibiting aerobic glycolysis through the β-catenin/c-Myc pathway by GONG Yu, LEI Jiao, ZHANG Ming

“…The renal histopathological changes were observed by HE staining and Paller scoring. ELISA was used to determine the contents of serum creatinine （Scr）， neutrophil gelatinase-associated lipocalin （NGAL） and lactate， and renal contents of hexokinase 2 （HK2）， lactate dehydrogenase A （LDHA）， IL-1β and IL-18. …”
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Diabetes Mellitus and HIV Infection among Newly Diagnosed Pulmonary Tuberculosis Patients in the North West Region of Cameroon: A Cross-Sectional Study by Leonard Fonkeng Sama, Sidoine Sadjeu, Thibau Flaurant Tchouangueu, Solange Dabou, Georges Ful Kuh, Omer Bebe Ngouateu, Michel Noubom

“…Sputum samples collected were acid-fast stained and examined macroscopically as well as inoculated for culture. …”
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Wnt3a Enhances Mesenchymal Stem Cell Engraftment and Differentiation in a Chronic Obstructive Pulmonary Disease Rat Model by Wu H, Zhong Y, Li Y, Zhou X, Zhao T, Wan D, Zhu Y, Zhang Z, Li X, Gan X

“…The engraftment of BMSC was detected by immunofluorescence staining. Statistical analysis was performed using GraphPad Prism 7.Results: We found that Wnt3a significantly enhanced the engraftment rate of BMSCs in the lungs of rats and further increased their differentiation rate into type II alveolar epithelial cells. …”
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Efficacy of Simple Eye Ointment, Polyethylene Cover, and Eyelid Taping in Prevention of Ocular Surface Disorders in Critically Ill Patients: A Randomized Clinical Trial by Mehdi Ahmadinejad, Esmat Karbasi, Yunes Jahani, Maryam Ahmadipour, Maryam Soltaninejad, Zahra Karzari

“…The eligible patients received the care procedure for seven days, and their corneas were examined daily for OSD by a portable slit lamp with fluorescein staining. Descriptive and analytical tests (ANOVA, chi-square, logistic regression, and zero-inflated Poisson regression) were used for statistical analysis by STATA14. …”
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Relationship between biometric characteristics with semen quality of buffalos (Bubalus bubalis) by Juanita Velásquez, Juan E. Duque, Juan D. Montoya, Alexandra Usuga

“…For each ejaculate, volume (VOL) and concentration (CON) were evaluated, and a computerized semen analysis system assessed sperm motility and kinetics, vitality, and abnormal sperm morphology (AM) by eosin-negrosin staining and the integrity of the plasma membrane by the hypoosmotic test. …”
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Evaluatıon of contrast enhancement intensity, pattern, and kinetics of breast lesions on contrast-enhanced spectral mammography by Ayşe Füsun Bekirçavuşoğlu, Sibel Kul, Süleyman Bekirçavuşoğlu, Dilara Atasoy, Merve Erkan

“…Breast magnetic resonance imaging (MRI) is based on the principle that hypervascular lesions are stained more intensely with contrast material than the breast glandular tissue. …”
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Perianal Paget’s Disease: The 17-Year-Experience of a Single Institution in Taiwan by Yu-Chen Wang, Anna Fen-Yau Li, Shung-Haur Yang, Hsiu-Hsun Ma, Wen-Yih Liang

“…Immunohistochemical staining showed CK7 (7/10 and 6/13), CK20 (6/10 and 10/13), CDX2 (6/10 and 12/13), and GCDFP-15 (3/10 and 0/13) positivities in primary and secondary PPD patients, respectively. …”
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