Chlamydia trachomatis Growth and Cytokine mRNA Response in a Prostate Cancer Cell Line by Ivan M. Petyaev, Naylia A. Zigangirova, Elena Y. Morgunova, Nigel H. Kyle, Elena D. Fedina, Yuriy K. Bashmakov

“…First of all, the initial stage of C. trachomatis propagation in CWR-R1 cells (24 hpi) was characterized by larger inclusion bodies and more intense, specific immunofluorescent staining of infected cells as compared with McCoy cells. …”
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Mechanism and Pharmacodynamic Substance Basis of Raw and Wine-Processed Evodia rutaecarpa on Smooth Muscle Cells of Dysmenorrhea Mice by Yeqian Liu, Hong Li, Lei Chen, Hongxia Zhao, Jian Liu, Shan Gong, Danfeng Ma, Chunming Chen, Shuiqing Zeng, Hongping Long, Weiqiong Ren

“…The cell contraction and the expression of P2X3 and Ca2+ in vitro were determined using immunofluorescence staining and laser confocal; ELISA was used for detection of PGE2, ET-1, and NO content after 7-hydroxycoumarin, chlorogenic acid, and limonin administered for 24 h. …”
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Nocardiosis in Renal Allograft Recipients by Chilaka Rajesh, Athul Thomas, Jeethu Joseph Eapen, Sabina Yusuf, Elenjickal Elias John, Anna T. Valson, Suceena Alexander, Vinoi George David, Joy Sarojini Michael, Santosh Varughese

“…In our study, eight patients required invasive diagnostic procedures such as bronchoalveolar lavage and CT-guided lung biopsy since initial Gram stain and sputum culture were negative. In conclusion, it is crucial to maintain a high level of suspicion and conduct thorough investigations among post renal transplant recipients. …”
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Creation and study of emmer (<i>Triticum dicoccum</i>) × triticale hybrids by O. G. Silkova, Y. N. Ivanova, P. I. Stepochkin

“…The aim of this work was to create and study hybrids of emmer T. dicoccum (Schrank) Schuebl. with hexaploid triticale using genomic in situ hybridization for staining of meiotic chromosomes and analysis of plant productivity elements in F4–F8. …”
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Bone cell differentiation and mineralization in wild-type and osteogenesis imperfecta zebrafish are compromised by per- and poly-fluoroalkyl substances (PFAS) by Francesca Tonelli, Cecilia Masiero, Carla Aresi, Camilla Torriani, Simona Villani, Guido Premoli, Antonio Rossi, Antonella Forlino

“…Morphometric measurements, confocal microscopy evaluation of operculum area delimited by the fluorescent preosteoblasts and mineral deposition analysis following alizarin red staining were employed. PFOS and the highest concentration of PFHxA significantly impaired standard length in both genotypes. …”
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Novel lncRNA LncMSTRG.11341.25 Promotes Osteogenic Differentiation of Human Bone Marrow Stem Cells via the miR-939-5p/PAX8 Axis by Feifei Ni, Jianjun Li, Qin Yin, Yangyang Chen, Zengwu Shao, Hong Wang

“…The interaction between LncMSTRG25 and PAX8 was verified using the RNA immunoprecipitation assay, RNA pull-down assays, silver staining, and the dual-luciferase reporter. The results show that LncMSTRG25 can function as a sponge to adsorb miR-939-5p, inducing the osteogenic differentiation of hBMSCs by activating PAX8. …”
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Protective Effects and Mechanisms of Dendrobium nobile Lindl. Alkaloids on PC12 Cell Damage Induced by Aβ25-35 by Yuan Liu, Tingting Pi, Xiaohui Yang, Jingshan Shi

“…The JC-1 fluorescent staining observed the effect of DNLA on the mitochondrial membrane potential (MMP) with inverted immunofluorescence microscopy. …”
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Anatomical and pathological findings in longlived female Bubalus bubalis by Rosaura Pérez-Gil, Yaritza Salas, Adelys Márquez, Oriana Gavidia

“…Later on, histological slices and routine HE stains were performed for examination using an optical microscope. …”
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Effects of Titanium Dioxide Nanoparticles on Chick Embryo: Immunomodulatory, Hepatic and Biochemical Alterations by Md. Sadequl Islam, Md. Nurul Amin, Mst. Deloara Begum

“…Histopathological changes were analysed using haematoxylin and eosin (H&E) staining, whereas amniotic fluid's biochemical properties were examined photometrically. …”
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Development of an sRNA-mediated conditional knockdown system for Chlamydia trachomatis by Janina Ehses, Kevin Wang, Asha Densi, Cuper Ramirez, Ming Tan, Christine Sütterlin

“…In proof-of-principle experiments, we induced functional knockdown of IncA, a fusion-mediating inclusion membrane protein, as shown with Western blots, loss of IncA staining at the inclusion membrane, and production of multiple chlamydial inclusions within an infected cell. …”
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Population Kinetics and Protein Profiles of Co-Cultured Adult and Fetus Rabbit Bladder Smooth Muscle Cells by Hayrunisa Kahraman Esen, Burcu Biltekin, Mevlit Korkmaz, B. Haluk Güvenç

“…Cells exhibited typical SMC morphology, confirmed by positive staining for α-SMA and MYH11. Results: Median cell counts of single cultures were significantly higher than co-cultures (P < .05), but cell viability was comparable (P > .05). …”
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Efficient and Rapid Microfluidics Production of Bio-Inspired Nanoparticles Derived from <i>Bombyx mori</i> Silkworm for Enhanced Breast Cancer Treatment by Muhamad Hawari Mansor, Zijian Gao, Faith Howard, Jordan MacInnes, Xiubo Zhao, Munitta Muthana

“…Haematoxylin & Eosin (H&E) staining indicated enhanced tumour necrosis in treated groups compared to controls. …”
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Assessment of hypoxia status in a rat chronic liver disease model using IVIM and T1 mapping by Wenlu Dong, Longyang Xiao, Ziwei Luo, Haiyang Yu, Lili Wang, Yuanxiang Gao, Zhiming Li

“…Liver specimens were subjected to immunohistochemical staining for the exogenous hypoxia marker pimonidazole and the endogenous hypoxia marker HIF-1α and scored semi-quantitatively. …”
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Inhibition of miR-182-5p Targets FGF9 to Alleviate Osteoarthritis by Yang Sun, Sanmao Su, Mengjun Li, Ang Deng

“…Hematoxylin–eosin (HE) and safranin O-fast Green (S–O) staining were utilized to access cartilage damage. …”
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Structural Characterization and Antioxidant Activity in Vivo of Polysaccharides from the Leaves of Hippophae rhamnoides L. by Niannian SONG, Yuxin YI, Xiaoping ZHANG, Bo YANG, Mangsier SAINAWAER, Abuduwaili AYITUXUN

“…Compared with the model group, the total superoxide dismutase, glutathione peroxidase, and catalaseactivities of HRLP in the medium dose group were significantly increased (P<0.05), while the content of malondialdehyde was significantly decreased (P<0.05). The HE staining results indicated that HRLP had protective effect on liver and kidney tissue cells and structure damage in mice. …”
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Unveiling the Effect of Age and IgE Level on Alopecia Areata: Insights from Comparative RNAseq Analysis by Liu H, Yang S, Xian H, Liu Y, Zhang Y, Chen Y, Xu Y, Liu J, Yang B, Luo Y

“…Immunohistochemical staining and qPCR was performed to validate these findings.Results: Our results revealed a more pronounced inflammatory immune infiltration in AA patients across all age groups compared to healthy controls. …”
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Therapeutic Approaches in Mitochondrial Dysfunction, Inflammation, and Autophagy in Uremic Cachexia: Role of Aerobic Exercise by Yumei Zhang, Yuqing Liu, Xiao Bi, Chun Hu, Feng Ding, Wei Ding

“…Renal function was assessed via serum creatinine and urea levels, and histological PAS and Masson staining were performed. Muscle wasting was determined based on grip strength, cross-sectional area (CSA), and MyHC protein expression. …”
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Successful External Cephalic Version: Factors Predicting Vaginal Birth by Pei Shan Lim, Beng Kwang Ng, Anizah Ali, Mohamad Nasir Shafiee, Nirmala Chandralega Kampan, Nor Azlin Mohamed Ismail, Mohd Hashim Omar, Zaleha Abdullah Mahdy

“…They required more operative deliveries, had more blood loss at delivery, a higher incidence of meconium-stained liquor, and more cord around the neck. Previous flexed breeches had a threefold increase in caesarean section rate compared to previous extended breeches (44.1% versus 15.2%, P=0.010). …”
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An In Vitro Model of Diabetic Retinal Vascular Endothelial Dysfunction and Neuroretinal Degeneration by Qiyun Wang, Xinyuan Zhang, Kaiyue Wang, Ling Zhu, Bingjie Qiu, Xiaosi Chen, Xiao Lin, Yao Nie

“…Apoptosis was detected using PI/Hoechst staining and TUNEL assay and quantified by ImageJ. …”
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Betaine Inhibits NLRP3 Inflammasome Hyperactivation and Regulates Microglial M1/M2 Phenotypic Differentiation, Thereby Attenuating Lipopolysaccharide-Induced Depression-Like Behavi... by Man Zhang, Xiao-Long Wang, Hui Shi, Lan-Qing Meng, Hong-Feng Quan, Lin Yan, Hui-Fang Yang, Xiao-Dong Peng

“…The expressions of cytokines and inflammatory mediators were detected by ELISA, HE staining, immunofluorescence, immunohistochemistry, and western blotting. …”
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