PropAngio study protocol: a neoadjuvant trial on the efficacy of propranolol monotherapy in cutaneous angiosarcoma—a proof of principle study by Neeltje Steeghs, Jos H Beijnen, Kimberley M Heinhuis, Nikki S IJzerman, Anne Miek Koenen, Winette T A van der Graaf, Rick L Haas, Alwin D R Huitema, Winan J van Houdt

“…The histological response will be determined as secondary endpoint, comparing the difference in proliferation index before and after propranolol by measuring the change in immunohistochemistry staining of Ki-67. The study will be considered positive when at least three patients have a response to propranolol.Ethics and dissemination Ethical approval was obtained from the Medical Ethical Committee of the Netherlands Cancer Institute. …”
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Impact of water deficit on the anatomical structure of more productive and less productive cashew trees (Anacardium occidentale L.) in Côte d’Ivoire by Abdoul Rahim Falk Ky, Mory Latif Konaté, N'Guessan François Kouamé

“…The technique of double staining with green-carmino made it possible to obtain anatomical sections of transversely cut samples under an optical microscope. …”
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Phenotypic Analysis of BrdU Label-Retaining Cells during the Maturation of Conducting Airway Epithelium in a Porcine Lung by Yuanyuan Jia, Xuehong You, Ningxia Ma, Hui Li, Guopan Liu, Ying Wang, Jing Xue, Juan Shi, Jun Wei, Yong Li, Jiali Yang, Xiaoming Liu

“…The BrdU-labeled epithelial cells also expressed keratin 14, mucin 5AC, or prosurfactant protein C (ProSP-C); among them, the keratin 14-positive cells were the most frequent BrdU-labeled epithelial cell type as determined by immunohistochemical and immunofluorescence staining. This study may provide valuable information on the biology and function of epithelial stem/progenitor cells in conducting airway of pigs and humans.…”
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Protective Effect of Black Bean-Whey Double Protein Diet on Intestinal Barrier Injury Induced by Lipopolysaccharide in Rats by Weihong MENG, Zhiming LI, Jiayu ZHANG, Xin SHU, Defu LIANG, Kejin ZHUANG, Dongjie ZHANG

“…The intestinal morphology of the rats was observed using hematoxylin and eosin staining. The expression of tight junction proteins was quantified using immunohistochemistry, and the TLR4 and MyD88 expression levels were determined using real-time fluorescence quantitative polymerase chain reaction. …”
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The immunological landscape of CCL26High invasive oral squamous cell carcinoma by Lingyun Liu, Lingyun Liu, Shuo Guan, Shuo Guan, Yizhuo Xue, Yizhuo Xue, Yijia He, Yijia He, Liang Ding, Yong Fu, Sheng Chen, Zhiyong Wang, Yi Wang

“…BackgroundOur previous study demonstrated that CCL26 secreted by cancer-associated fibroblasts (CAF) promoted the invasive phenotype of oral squamous cell carcinoma (OSCC), however, more comprehensive clinical expression patterns of CCL26 and its role in immunotherapy remains ambiguous.MethodsCCL26 levels in different cancer and normal tissues were analyzed and validated in 67 OSCC patients through immunohistochemical staining (IHC). The clinical spatial distribution pattern of CCL26 in tumor microenvironment was determined, and its clinical outcomes were investigated. …”
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Cisplatin and lipopolysaccharide co-administration for renal injury model in mice by Sae-Byeok Hwang, Soon-Suk Kang, Yeonmi Lee, Eunju Kang

“…Additionally, hematoxylin and eosin (H&E) and Masson’s trichrome (MT) staining were performed to assess histological changes. …”
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Pirfenidone Alleviates Choroidal Neovascular Fibrosis through TGF-β/Smad Signaling Pathway by Chuang Gao, Xin Cao, Lili Huang, Yueqi Bao, Tao Li, Yue Di, Liucheng Wu, Yu Song

“…The fibrosis area was monitored by staining the collagen type I (Col-I). Western blotting was used to analyze the expression of TGF-β2, Smad 2/3, phosphorylated Smad 2/3 (p-Smad 2/3), and α-smooth muscle actin (α-SMA). …”
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Elucidating genetic variability between randomly bred domestic cats and Persian domestic cats from different geographical locations using microsatellite markers by Shirin Mahmoodi, Ali Hojabr Rajeoni, Mehrshad Zeinolabedini, Arash Javanmard, Mohammad Hossein Banabazi

“…DNA extraction was performed using tail hair root, PCR and electrophoresis, and gel staining was performed according to routine laboratory protocol. …”
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Activin levels correlate with lymphocytic infiltration in epithelial ovarian cancer by Elizabeth T. Evans, Emily F. Page, Alex Seok Choi, Zainab Shonibare, Andrea G. Kahn, Rebecca C. Arend, Karthikeyan Mythreye

“…Immunohistochemistry analysis revealed a positive correlation of CD8 and FOXP3 staining with activin A at the protein level in both primary and metastatic epithelial ovarian cancer samples. …”
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Acupuncture for patients with type 2 diabetes mellitus with dry eye: protocol for a systematic review and meta-analysis by Ting Li, Xin Xu, Juan Wei, Qingbo Wei, Zhouyu Xie, Xiangyu Bian, Yunchuan Wu

“…The primary outcomes will include Schirmer’s I test, breakup time, corneal fluorescein staining and ocular surface disease index scores. Secondary results will include visual analogue scale scores for ocular symptoms and any adverse events related to acupuncture. …”
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Genome Sequencing Providing Molecular Evidence of Tetrapolar Mating System and Heterothallic Life Cycle for Edible and Medicinal Mushroom <i>Polyporus umbellatus</i> Fr. by Shoujian Li, Youyan Liu, Liu Liu, Bing Li, Shunxing Guo

“…Additionally, the number of sterigma and basidiospores on each basidium was examined using scanning electron microscopy, while the nuclei of basidiospores and basidia at various developmental stages were analyzed through DAPI staining. This research clarifies the heterothallic life cycle of <i>P. umbellatus</i>. …”
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Monoclonal Gammopathy of Undetermined Significance with Amyloid Deposition in the Lung and Non-Amyloid Eosinophilic Deposition in the Brain: A Case Report by Francois Abi-Fadel, Nisarg R. Desai, Gita Vatandoust, Rabih Said, Aaron Gottesman, Terenig Terjanian

“…Brain biopsy was positive for eosinophilic material (similar to the lungs) but negative for Thioflavin T stain. The patient's clinical status continued to deteriorate with cold cyanotic fingers developing on day 12 and a health care acquired pneumonia, respiratory failure, and fungemia on day 18. …”
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A <i>SMALL AUXIN UP-REGULATED RNA</i> Gene Isolated from Watermelon (<i>ClSAUR1</i>) Positively Modulates the Chilling Stress Response in Tobacco via Multiple Signaling Pathways by Duo Wang, Gangli Ma, Jia Shen, Xinyang Xu, Weisong Shou, Zhengying Xuan, Yanjun He

“…Moreover, qRT-PCR and promoter::β-glucuronidase (<i>GUS</i>) staining assays revealed that the expression of <i>ClSAUR1</i> is significantly upregulated in response to exogenous abscisic acid (ABA) and chilling stress. …”
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Synergistic toxicity of nanoplastics and Helicobacter pylori on digestive system in mice by Xiaolin Zhang, Yunqing Wang, Xiaohan Tong, Boqing Li, Sisi Liu, Yingzi Cui, Wenke Wang, Zhiqin Li, Chunlei Ma, Ying Zhang

“…Transmission electron microscopy confirmed the accumulation of AuPS-NPs (Au-core polystyrene nanoplastics) in the stomach, colon, and liver, while hematoxylin and eosin staining revealed dose-dependent pathological damage in these tissues. …”
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Synchronous Occurrence of Splenic Pleomorphic Mantle Cell Lymphoma and Esophageal Adenocarcinoma with Overexpression of BCL1 Protein by Dominik Dabrowski, Roberto F. Silva, Michael Constantinescu, Rodney E. Shackelford, Nestor Dela Cruz, Eric X. Wei

“…Despite numerous immunohistochemical stainings studied, only BCL1 was demonstrated to have partial overlap in both tumors. …”
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Paraoxonase and Arylesterase Activities, Lipid Profile, and Oxidative Damage in Experimental Ischemic Colitis Model by Ethem Unal, Cengiz Eris, Bülent Kaya, Hafize Uzun, Faruk Cavdar, Murat Yildar, Ali Riza Kiziler, Birsen Aydemir, Pembegul Gunes, Riza Kutanis, Izzet Titiz

“…Histopathological evaluation was done under light microscopy, after sectioning and staining with hematoxyline and eosin. Statistical analysis was conducted using Student’s t-test and Mann-Whitney U test, and P<0.05 was considered as statistically significant. …”
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Endoplasmic reticulum stress-related CLIP4 plays a procarcinogenic role in hepatocellular carcinoma: an integrated analysis by Anqi Wang, Sitong Yan, Weijia Jiang, Xiang Chen, Yuhan Huang, Xiangyu Zu, Xiao Du, Lulu Fan, Jiatao Liu, Guoping Sun

“…CCK-8, colony formation, EdU staining, wound-healing, Transwell, western blotting and RT-qPCR were used to detect the effects of CLIP4 on the proliferation, migration and epithelial-mesenchymal transition (EMT) of HCC cells. …”
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Insular infraorbital neurovascular pedicle labial salivary gland transplantation for the treatment of severe dry eye disease: an IDEAL stage 0, 1 and 2a study by Jing Zhou, Xiaohong Chen, Mei Li, Ke Yang, Ying Jie, Yinghui Wang, Yingshi Piao, Yun Cheng

“…We designed a modified LSGT, called insular infraorbital neurovascular pedicle LSGT, and evaluated the postoperative efficacy.Design This is a prospective, single-centre, self-contained study.Setting The research was conducted at Beijing Tongren Hospital, Capital Medical University from July 2019 to March 2024.Participants Eight patients (nine eyes) with severe dry eye disease (DED) were enrolled in this study.Interventions All patients underwent insular infraorbital neurovascular pedicle LSGT and were followed up for at least 6 months postoperatively.Main outcome measure Key evaluation indices were best-corrected visual acuity (BCVA), Ocular Surface Disease Index (OSDI) score, tear break-up time (TBUT), Corneal Fluorescence Staining (CFS) score, and Schirmer I test (SIT).Results With a mean follow-up of 17.56±11.72 months, BCVA improved in four eyes and stabilized in five. …”
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Construction of an anaplastic thyroid cancer stratification signature to guide immune therapy selection and validation of the pivotal gene HLF through in vitro experiments by Li Pengping, Yin Kexin, Xie Yuwei, Sun Ke, Li Rongguo, Wang Zhenyu, Jin Haigang, Wang Shaowen, Huang Yuqing

“…In vitro experiments, such as western blot and immunofluorescence staining, are performed.ResultsUsing a set of five genes uniquely expressed across various types of thyroid cancer, we developed a machine-learning model to distinguish each type within the GEO dataset of thyroid cancer patients (GSE60542, GSE76039, GSE33630, GSE53157, GSE65144, GSE29265, GSE82208, GSE27155, GSE58545, GSE54958, and GSE32662). …”
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Determining the mechanism of Shuxuening injection against liver cirrhosis through network pharmacology and animal experiments by Qiyao Liu, Tingyu Zhang, Yongan Ye, Xin Sun, Huan Xia, Xu Cao, Xiaoke Li, Wenying Qi, Yue Chen, Xiaobin Zao

“…Serum and liver tissues of the mice were collected for the detection of indicators, pathological staining, and expression analysis of hub targets using quantitative real-time polymerase chain reaction (qRT-PCR). …”
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