Inflammatory-Driven Angiogenesis in Bone Augmentation with Bovine Hydroxyapatite, B-Tricalcium Phosphate, and Bioglasses: A Comparative Study by Vlad M. Anghelescu, Ioana Neculae, Octavian Dincă, Cristian Vlădan, Claudiu Socoliuc, Mirela Cioplea, Luciana Nichita, Cristiana Popp, Sabina Zurac, Alexandru Bucur

“…Endothelial cell analysis was done by analysing vascularization with PECAM/CD31 and VEGF and fibrosis with collagen IV, laminin, and osteonectin stains. Statistical analysis was realized by descriptive analysis which was completed with the kurtosis and skewness as well as the Kruskal-Wallis and Mann-Whitney statistical tests. …”
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FOSL2 activates TGF‐β1‐mediated GLUT1/mTOR signaling to promote diabetic kidney disease by Xuelin He, Min Xia, Guanghui Ying, Qien He, Zhaogui Chen, Li Liu, Qiao Zhang, Jianxin Cai

“…Method The kidney tissues of DKD mice induced by STZ and a high‐fat diet were subjected to PAS and Masson's staining. Glomerular mesangial cells (MCs) were treated with high glucose (HG) or normal glucose (NG). …”
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Burn wound healing using adipose-derived mesenchymal stem cells and manganese nanoparticles in polycaprolactone/gelatin electrospun nanofibers in rats by Azin Shahmohammadi, Hadi Samadian, Saeed Heidari Keshel, Khodabakhsh Rashidi, Amir Kiani, Masoud Soleimani, Farjam Goudarzi

“…The wound closure rate, IL-1β, and IL-6 level, hydroxyproline, and glycosaminoglycans content were measured, and the hematoxylin and eosin, Masson’s trichrome, and immunohistochemistry stainings were carried out. Results: Based on the results, in Mn+S (ADSCs+PCL/Gel/5%MnO2-NPs nanofiber) and N+S (ADSCs+PCL/Gel nanofiber) groups, the IL-6 and IL-1β levels were reduced, and the percentage of wound closure, glycosaminoglycans, and hydroxyproline content were increased compared to the control group (P<0.05). …”
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Modality Specific CBAM-VGGNet Model for the Classification of Breast Histopathology Images via Transfer Learning by Areesha Ijaz, Basit Raza, Iqra Kiran, Abdul Waheed, Aadil Raza, Habib Shah, Sulaiman Aftan

“…In this study, we have proposed a novel modality specific CBAM-VGGNet model for classifying H and E stained breast histopathology images. Instead of using pre-trained models on ImageNet, we have trained VGG16 and VGG19 models on the same domain cancerous histopathology datasets, which are then used as fixed feature extractors. …”
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Soluble Receptor for Advanced Glycation End Products: A Protective Molecule against Intramyocardial Lipid Accumulation in Obese Zucker Rats? by Elena Dozio, Elena Vianello, Francesco Bandera, Erika Longhi, Stefano Brizzola, Manuela Nebuloni, Massimiliano M. Corsi Romanelli

“…Heart and serum samples were collected from 10 lean (L) and 10 obese (OB) Zucker rats. Oil red staining was used to detect lipids on frozen heart sections. …”
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Antimicrobial susceptibility patterns of pathogens isolated from clinical specimens at a tertiary care hospital in Bangladesh, 2020–2023 by Mohammad Kamruzzaman, Fahim Alam Nobel, Saiful Islam, Amiyo Haider, Mohammad Nasir Uddin, Mohammod Johirul Islam

“…The isolates were characterized through colony morphology, gram staining, and biochemical assays. Their antibiotic susceptibility patterns were determined using the Kirby-Bauer disk diffusion method. …”
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Growth of the prefrontal cortical glioblastoma altered cognitive and emotional behaviors via mediating miRNAs and GABA-A receptor signaling pathways in rats by Sepide Nateghi, Ameneh Rezayof, Fatemeh Kouhkan, Ladan Delphi, Yavar Bagheri Davisaraei, Fatemeh Rostami, Fatemeh Tirgar, Houri Sepehri

“…The implantation of C6 cells into the mPFC induced GBM in this brain region (referred to as the mPFC-GBM) in male Wistar rats via stereotaxic surgery, as confirmed by Magnetic Resonance Imaging (MRI), and Hematoxylin and Eosin (H&E) staining. Repeated microinjections of muscimol, a potent GABAA receptor agonist, directly into the mPFC-GBM (1 µg/rat/2.5 μl) following tumor induction decreased tumor volume and weight, resulting in an increased survival rate. …”
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Repurposing of the Nootropic Drug Vinpocetine as an Analgesic and Anti-Inflammatory Agent: Evidence in a Mouse Model of Superoxide Anion-Triggered Inflammation by Yuri Lourenco-Gonzalez, Victor Fattori, Talita P. Domiciano, Ana C. Rossaneis, Sergio M. Borghi, Tiago H. Zaninelli, Catia C. F. Bernardy, Jose C. Alves-Filho, Thiago M. Cunha, Fernando Q. Cunha, Rubia Casagrande, Waldiceu A. Verri

“…In the KO2 model, vinpocetine dose-dependently reduced pain-like behaviors (spontaneous pain and hyperalgesia), paw edema, and neutrophil and mononuclear cell recruitment to the paw skin (assessed by H&E staining, fluorescence, and enzymatic assays) and to the peritoneal cavity. …”
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Effects of non-contact electric fields on the kidneys and livers of tumour-bearing rats [version 6; peer review: 1 approved, 2 approved with reservations, 1 not approved] by Luthfi Nurhidayat, Ahmad Ghitha Fadhlurrahman, Firman Alamsyah, Warsito Purwo Taruno, Nisrina Firdausi, Subekti Evi Dwi Nugraheni, Rarastoeti Pratiwi

“…Histopathological cross-sections of the kidneys and livers were made using the paraffin method and Hematoxylin-Eosin staining. Histological scoring used the post-examination masking method with 100 visual fields per group. …”
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Successful Multimodal Management of an Aggressive Functional Gonadotropic Pituitary Macroadenoma by Jeffrey J. Feng, MS, Sophie M. Cannon, MD, Stephanie K. Cheok, MD, Mark S. Shiroishi, MD, Kyle M. Hurth, MD, PhD, Anna J. Mathew, MD, Gabriel Zada, MD, John D. Carmichael, MD

“…Immunohistochemistry results following endoscopic transsphenoidal resection confirmed dominant staining for steroidogenic factor-1, FSH, and LH. Postoperatively, the patient’s FSH level decreased, whereas the LH level normalized within 1 week. …”
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Genetic Signatures: CD44 Single-Nucleotide Polymorphisms Affect Cell Surface Expression and Elevate Risk in Head and Neck Squamous Cell Carcinoma by Muhammad Kashif, Shah Jahan, Sadia Minhas, Ali Amar, Romeeza Tahir, Haseeb Nisar, Faheem Shehzad, Abdul Hanan Nagi, Nadeem Afzal

“…Other notable findings included significant associations between CD44 rs13347 genotype and age (P = .031), number of CD44-positive tumor cells (P = .049), CD44 staining intensity (SI; P = .039), and CD44 immunoreactivity score (IRS) status (P = .019).CONCLUSIONThe T allele and homozygous TT genotype of CD44 rs13347 SNP were associated with increased susceptibility to HNSCC and decreased proportion of CD44-positive tumor cells, low SI, and reduced IRS.…”
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Inhibition of mmu_circ_0009303 improves metabolic dysfunction-associated steatotic liver disease by regulating lipid metabolism and oxidative stress by Ju Zhou, Wu Li, Xiaowei Chi, Dingchun Li, Chunxia Yang, Zhiwen Duan

“…We used a bioinformatics approach to identify potential targets and established an in vitro model of MASLD. Oil red O staining, cell transfection and dual-luciferase reporter assay were used to determine the role of mmu_circ_0009303. …”
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Metabolomics analysis of anaphylactoid reactions induced by Xueshuantong injection in normal and immunocompromised mice by Xiaoqian Guo, Xiaoqian Guo, Chi Zhang, Chi Zhang, Yingyu Li, Yingyu Li, Yingyu Li, Wen Wen, Wen Wen, Wen Wen, Yinghui He, Feng Tang, Chunming Chen, Chao Hu, Linqi OuYang, Wenlong Liu, Wenlong Liu, Zhenhua Zhu, Hongyu Liu

“…Behavioral responses, auricle blue staining, and Evans blue (EB) exudation were used as indices to evaluate the sensitization of XSTI on both normal and immunocompromised mice. …”
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Prognostic Features and Potential for Immune Therapy in Metastatic Mismatch Repair‐Deficient Colorectal Cancer: A Retrospective Analysis of a Large Consecutive Population‐Based Pat... by Erkki‐Ville Wirta, Hanna Elomaa, Jukka‐Pekka Mecklin, Toni T. Seppälä, Marja Hyöty, Jan Böhm, Maarit Ahtiainen, Juha P. Väyrynen

“…Histological tumor parameters were evaluated from hematoxylin‐ and eosin‐stained whole‐slide samples. CD3 and CD8 immunohistochemistry were analyzed to calculate T‐cell densities in the tumor center and invasive margin, and G‐cross function values to estimate cancer cell–T‐cell co‐localization. …”
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<i>Endarachne binghamiae</i> Extract Ameliorates Inflammatory Responses in Macrophages Through Regulation of MAPK, NF-kB and PI3K/AKT Pathways, and Prevents Acute Lung Injury in Mi... by Sang-Hoon Lee, Sang-Seop Lee, Ga-Young Lee, Seung-Yun Han, Dong-Sub Kim, Bong-Ho Lee, Yung-Choon Yoo

“…Furthermore, the inhibitory effect of EB-WE on ALI was apparently confirmed in histological examination through lung tissue staining. Taken together, it is clear that EB-WE has potential activity to effectively ameliorate the inflammatory responses in macrophages through down-regulation of MAPK, NF-kB, and PI3K/AKT activation, and suppress acute lung injury induced by LPS. …”
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Theranostic nanoemulsions suppress macrophage-mediated acute inflammation in rats by Riddhi Vichare, Yalcin Kulahci, Rebecca McCallin, Fatih Zor, Fatma Nurefsan Selek, Lu Liu, Caitlin Crelli, Anneliese Troidle, Michele Herneisey, James M. Nichols, Andrew J. Shepherd, Vijay S. Gorantla, Jelena M. Janjic

“…This consequently decreased macrophage infiltration at the CFA-induced inflammation site in both sexes compared to drug-free nanoemulsion, as demonstrated by NIRF imaging, H&E staining, and immunofluorescence. Taken together, our results indicated that the anti-inflammatory efficacy of curcumin was significantly improved when directly delivered to pro-inflammatory macrophages via theranostic nanoemulsion. …”
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Generation of Insulin-Producing Cells from Canine Adipose Tissue-Derived Mesenchymal Stem Cells by Takahiro Teshima, Keiji Okamoto, Kazuho Dairaku, Tomokazu Nagashima, Masaki Michishita, Ryohei Suzuki, Hirotaka Matsumoto, Hidekazu Koyama

“…The generated IPCs under 3D culture condition were stained with dithizone and anti-insulin antibody. …”
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Integrated serum metabolomics and network pharmacology reveal molecular mechanism of Qixue Huazheng formula on peritoneal fibrosis by Xiaohui Meng, Xiaohui Meng, Li Sheng, Yongqing You, Yongqing You, Huibo Dai, Manshu Yu, Funing Wang, Funing Wang, Ziren Zhou, Ziren Zhou, Yun Shan, Meixiao Sheng

“…Western blotting (WB), quantitative real-time PCR (qRT-PCR), TdT-mediated dUTP Nick-End Labeling (TUNEL) staining, and flow cytometry were conducted.ResultsQXHZF had potent therapeutic efficacy against PF according to WB, qRT-PCR, and pathological section examination. …”
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Dyssynchronous heart failure models in canines: New insights into electrocardiographic, echocardiographic and histological features by Han Jin, Shengwen Yang, Hao Huang, Sijing Cheng, Pengkang He, Sixian Weng, Min Gu, Hongxia Niu, Wei Hua, Yiran Hu, Hui Li

“…Three weeks of follow‐up were conducted to observe the changes in cardiac function and myocardial staining was performed at the end of the experiment. …”
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The Immunohistochemical Prognostic Value of Nuclear and Cytoplasmic Silent Information Regulator 1 Protein Expression in Saudi Patients with Breast Cancer by Bayan Alharbi, Alia Aldahlawi, Mourad Assidi, Fatemah Basingab, Kawther Zaher, Jehan Alrahimi, Sara Mokhtar, Jaudah Al-Maghrabi, Abdelbaset Buhmeida, Kaltoom Al-Sakkaf

“…Methods: A total of 407 BC formalin-fixed paraffin-embedded (FFPE) samples were collected from King Abdulaziz University Hospital, Saudi Arabia. SIRT1 was stained on tissue microarray slides using automated immunohistochemistry. …”
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