Integrative machine learning frameworks to uncover specific protein signature in neuroendocrine cervical carcinoma by Tao Shen, Tingting Dong, Haiyang Wang, Yi Ding, Jianuo Zhang, Xinyi Zhu, Yeping Ding, Wen Cai, Yalan Wei, Qiao Wang, Sufen Wang, Feiyun Jiang, Bin Tang

“…The diagnostic effect of kNsDEPs was validated by a set of predictive models and immunohistochemical staining method. The dysregulation patterns of kNsDEPs were further investigated in other neuroendocrine carcinomas. …”
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Increased TSPO alleviates neuropathic pain by preventing pyroptosis via the AMPK-PGC-1α pathway by Baolong Li, Kaiming Yu, Xiongyao Zhou, Jialu Sun, Le Qi, Weiye Li, Tuo Yang, Weizhen Li, Ningning Wang, Xiaosong Gu, Shusen Cui, Rangjuan Cao

“…Pharmacological upregulation of TSPO, but not its downregulation, effectively alleviated SNI-induced pain hypersensitivity, improving mitochondrial function and reducing pyroptosis. Immunofluorescence staining confirmed that TSPO was primarily localized in astrocytes, and its expression mirrored the protective effects on mitochondrial health and pyroptosis prevention. …”
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Excessive copper induces lignin biosynthesis in the leaves and roots of two citrus species: Physiological, metabolomic and anatomical aspects by Xin Zhou, Xing Xiong, Fei Lu, Wenqing Shi, Yu Zhou, Ningwei Lai, Li-Song Chen, Zeng-Rong Huang

“…The citrus roots are more prominent in the lignin precursor biosynthesis under Cu toxicity than citrus leaves. The histochemical staining supported that Cu stress improved the deposition of both guaiacy and syringy lignin units in citrus roots. …”
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Therapeutic Effects of GDF6-Overexpressing Mesenchymal Stem Cells through Upregulation of the GDF15/SIRT1 Axis in Age-Related Hearing Loss by Jiali Liu, Haisen Peng, Yuehui Liu, Chunhua Li, Wen Xie

“…Hearing function and loss of outer hair cells (HCs) in ARHL rats were measured using the auditory brainstem response and cochlear silver nitrate staining, respectively. MSC proliferation was evaluated with the Cell Counting Kit-8 assay. …”
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An automated platform for simultaneous, longitudinal analysis of engineered neuromuscular tissues for applications in neurotoxin potency testing by Jacob W. Fleming, Molly C. McCloskey, Kevin Gray, David R. Nash, Vincent Leung, Christos Michas, Shawn M. Luttrell, Christopher Cavanaugh, Julie Mathieu, Shawn Mcquire, Mark Bothwell, David L. Mack, Nicholas A. Geisse, Alec S.T. Smith

“…Histological analysis revealed characteristic punctate acetylcholine receptor staining co-localized with the presynaptic marker synaptic vesicle protein-2. …”
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S100A8/A9 Molecular Complexes Promote Cancer Migration and Invasion via the p38 MAPK Pathway in Nasopharyngeal Carcinoma by Ning Xu, Bei-Bei Zhang, Xia-Ning Huang, Xiang Yi, Xue-Min Yan, Yan Cai, Qin He, Zi-Jian Han, Yuan-Jiao Huang, Wei Liu, Ai-Jun Jiao

“…Immunohistochemical staining was performed in different tissues and analyzed by the Mann–Whitney U test statistically. …”
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Irisin Attenuates Hepatic Stellate Cell Activation and Liver Fibrosis in Bile Duct Ligation Mice Model and Improves Mitochondrial Dysfunction by Thuy Linh Lai, So Young Park, Giang Nguyen, Phuc Thi Minh Pham, Seon Mee Kang, Jeana Hong, Jae-Ho Lee, Seung-Soon Im, Dae-Hee Choi, Eun-Hee Cho

“…In vivo, irisin ameliorated the elevated liver-to-body weight ratio induced by BDL and alleviated liver fibrosis, as evidenced by Masson’s trichrome staining. Irisin also improved mitochondrial dysfunction induced by BDL surgery. …”
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Upregulation of GSTP1 mediated by chimeric TFE3 promotes TFE3-tRCC progression by targeting JNK signaling pathway by Weixu Chen, Mengtong Wu, Lin Du, Changhua Fang, Hao Wang, Wendi Wang, Chengwei Zhang, Hongqian Guo, Gutian Zhang

“…MTT and colony formation assays were used to detect cell proliferation. Annexin V/PI staining was used to evaluate cell apoptosis. Transwell assays were used to evaluate in vitro cell migration and invasion. …”
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Radix Scutellariae Ameliorates Stress-Induced Depressive-Like Behaviors via Protecting Neurons through the TGFβ3-Smad2/3-Nedd9 Signaling Pathway by Fan Zhao, Chenyiyu Zhang, Dong Xiao, Weihua Zhang, Liping Zhou, Simeng Gu, Rong Qu

“…The neuroprotective effect of RS was evaluated with the expression of hippocampal neuron-related markers and apoptosis-associated proteins by Nissl staining, immunohistochemistry, and western blot. Transforming growth factor-β3 (TGFβ3) pathway-related proteins were detected by western blot. …”
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Characterization of a chemically induced osteoarthritis model in zebrafish by Gongyi Xiao, Jin Qin, Huiping Yang, Qizhi Song, Ruobin Zhang, Junlan Huang, Yuexi Mou, Wen Liu, Xianding Sun, Mao Nie

“…Subsequent analyses included histological staining, immunohistochemistry, OA research society international (OARSI) scoring, and real-time in vivo imaging were performed at 7, 14, and 28 days post injection. …”
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Polymerase delta-interacting protein 2 mediates brain vascular permeability by regulating ROS-mediated ZO-1 phosphorylation and localization at the interendothelial border by Keke Wang, Hongyan Qu, Ruinan Hu, Bernard Lassègue, Douglas C. Eaton, Chang Song, Jianjun Mu, Kathy K. Griendling, Marina S. Hernandes

“…In vitro permeability assays demonstrated that Poldip2 knockdown suppressed TNF-α-induced endothelial cell (EC) permeability. Immunofluorescence staining showed that Poldip2 depletion prevented TNF-α-induced ZO-1 disruption at interendothelial junctions. …”
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Single‐cell dissection reveals immunosuppressive F13A1+ macrophage as a hallmark for multiple primary lung cancers by Chenglin Yang, Jiahao Qu, Jingting Wu, Songhua Cai, Wenyi Liu, Youjun Deng, Yiran Meng, Liuqing Zheng, Lishen Zhang, Li Wang, Xiaotong Guo

“…Methods We performed a comparative single‐cell RNAsequencing (scRNA‐seq) analysis on tumour and adjacent para‐tumour tissues fromMPLC and SPLC patients to comparatively evaluate their immunological landscapes.Additionally, multiplex immunofluorescence (mIF) staining and independentvalidation datasets were used to confirm findings. …”
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Carboxylesterase 4A Inhibits the Malignant Biological Behavior of Nasopharyngeal Carcinoma via the PI3K/AKT Pathway by Qiaoli Chen MS, Quanxiang Hao MBBS, Yanping Yang MBBS, Limei Li PhD, Danping Li MS, Ran Zhao MS, Wanqi Wei MBBS, Lixian Deng MBBS, Jiaming Su MBBS, Ziyuan Liang MBBS, Shiyue Tang MS, Yaomin Lu MBBS, Yushan Liang PhD, Zhe Zhang PhD, Xiaoying Zhou PhD, Xue Xiao PhD, Ping Li PhD, Yi Huang PhD, Weilin Zhao PhD

“…Method A standardized mean difference (SMD) analysis was used to analyze the dysregulation of CES4A based on the gene expression omnibus (GEO) database. qRT-PCR and immunohistochemical staining (IHC) were used to identify the mRNA and protein levels of CES4A in NPC cell lines and tissues, respectively. …”
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Sulforaphane Wrapped in Self-Assembled Nanomicelle Enhances the Effect of Sonodynamic Therapy on Glioma by Yihong Li, Xuejie Yang, Zhen Wei, Heng Niu, Liyang Wu, Caijing Chen, Huina Liu, Ting Cai, Huadong Fan

“…In animal experiments, besides a significant reduction in tumor volume and a delay in losing body weight, H&E staining showed a massive infiltration of neutrophils adjacent to the tumor sites, indicating this novel nanocomplex SFN@RB@SPM can synergistically augment SDT efficacy, partially by enhancing the antitumor function of innate immunity.…”
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Voluntary Forelimbs Exercise Reduces Immobilization-Induced Mechanical Hyperalgesia in the Rat Hind Paw by Kumiko Ishikawa, Satoshi Oga, Kyo Goto, Junya Sakamoto, Ryo Sasaki, Yuichiro Honda, Hideki Kataoka, Minoru Okita

“…To investigate the abnormality of primary sensory neurons and central sensitization, the number of calcitonin gene-related peptide-positive cells in the dorsal root ganglion and the expression level of calcitonin gene-related peptide in the spinal dorsal horn were analyzed by immunohistochemical staining. Immobilization-induced mechanical hyperalgesia was inhibited in the EX group compared to the IM group at three weeks after immobilization. …”
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The histological development of the fetal human inferior colliculus during the second trimester by Reetuparna Nanda, Mihail Bota, Jaikishan Jayakumar, Jaikishan Jayakumar, Suresh S, S. Lata, E. Harish Kumar, Chitra Srinivasan, Sudha Vasudevan, Kumutha Jayaraman, Mohanasankar Sivaprakasam, Mohanasankar Sivaprakasam, Mohanasankar Sivaprakasam, Richa Verma

“…The volumetric changes in the IC were further evaluated at the cellular level using serial Nissl-stained sections, as well as glial fibrillary acidic proteins (GFAP) and calretinin immunohistochemistry. …”
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Mechanism of mTOR/RILP-regulated autophagic flux in increased susceptibility to myocardial ischemia-reperfusion in diabetic mice by Jiyao Zhao, Wei Shi, Yan Zheng, Junjie Wang, Muzhao Yuan, Yultuz Anwar, Yuxuan He, Haiping Ma, Jianjiang Wu

“…This was followed by the validation of potential mechanisms of action between impaired autophagic flux and increased susceptibility at the cellular and animal levels, respectively.ResultsAfter I/RI in diabetic myocardium, there was a significant increase in the number of CM1 subgroups and a specific downregulation of 239 autophagy-related genes led by RILP. HE staining revealed that myocardial injury was exacerbated in diabetic mice subjected to I/RI. …”
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Effect of afzelin on inflammation and lipogenesis in particulate matter-stimulated C. acnes-treated SZ95 sebocytes by Ji Yeon Hong, Yong Hee Choi, Yoon Jin Roh, Mi-Kyung Lee, Christos C. Zouboulis, Kui Young Park

“…Intracellular lipid droplet formation was investigated using Nile Red O staining.ResultsPM treatment upregulated the mRNA and protein expression levels of inflammatory cytokine and lipogenic genes in C. acnes-treated SZ95 sebocytes. …”
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Feasibility of an indocyanine green-hyaluronic acid mixture (LuminoMark™) for targeting suspicious axillary lymph nodes in patients with breast cancer by Jeeyeon Lee, Byeongju Kang, Jin Hyang Jung, Hye Jung Kim, Won Hwa Kim, Jung Dug Yang, Joon Seok Lee, Yee Soo Chae, Soo Jung Lee, In Hee Lee, Ji-Young Park, Nora Jee-Young Park, Ho Yong Park

“…Abstract Purpose During breast cancer surgery, the use of dyes such as indigo carmine, methylene blue, or indocyanine green (ICG) for targeting axillary lymph nodes (ALNs) under ultrasound guidance can result in rapid diffusion, complicated tissue differentiation, and disruption of staining. LuminoMark™, a novel ICG-hyaluronic acid mixture, can provide real-time visualization and minimize dye spread, thereby ensuring a clear surgical field. …”
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CCL4 Affects Eosinophil Survival via the Shedding of the MUC1 N-Terminal Domain in Airway Inflammation by Yoshiki Kobayashi, Chu Hong Hanh, Naoto Yagi, Nhi Kieu Thi Le, Yasutaka Yun, Akihiro Shimamura, Kenta Fukui, Akitoshi Mitani, Kensuke Suzuki, Akira Kanda, Hiroshi Iwai

“…Nasal polyp samples from patients with CRSwNP and BEAS-2B airway epithelial cells, coincubated with purified eosinophils, were stained with two MUC1 antibodies. To confirm the involvement of CCL4, an anti-CCL4 neutralizing antibody or recombinant CCL4 was used as needed. …”
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