Tear neuropeptides are associated with clinical symptoms and signs of dry eye patients by Yirou Zhang, Hong Zhang, Xingyu Zhu, Han Ye, Kan Yang, Xujiao Zhou, Jiaxu Hong

“…First time of noninvasive breakup time (NIBUT-1st), mean time of noninvasive breakup time (NIBUT-avg), tear meniscus height (TMH), Schirmer test, corneal fluorescein staining (CFS) score and ocular surface disease index (OSDI) were recorded. …”
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Remote and Anesthetic-Induced Myocardial Preconditioning Is Preserved in Atherosclerotic LDL Receptor-/- Mice In Vivo by Walter Petermichl, Kathrin Eglmeier, Henriette Hesse, Michael Gruber, Bernhard Graf, Andre Bredthauer, Andreas Redel

“…The area at risk and infarct size were determined by Evans Blue and triphenyltetrazolium chloride staining. Results. Histopathological examination showed atherosclerosis in high-fat atherogenic fed LDLR-/- mice, and the vessel relaxation capacity was significantly reduced compared to wild-type mice. …”
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Unraveling TGF-β1’s Role in Mediating Fibrosis and Cell Death in Feline Kidney Cells by Chanyanuch Intachat, Phongsakorn Chuammitri, Benjaporn Sornpet, Prapas Patchanee, Nawin Manachai, Kakanang Piyarungsri

“…Immunohistochemistry staining of TGF-β1 and MAPK was performed on feline kidney tissue. …”
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Koumine Attenuates Neuroglia Activation and Inflammatory Response to Neuropathic Pain by Gui-Lin Jin, Sai-Di He, Shao-Mei Lin, Li-Mian Hong, Wan-Qing Chen, Ying Xu, Jian Yang, Su-Ping Li, Chang-Xi Yu

“…The analgesic and anti-inflammatory effects of koumine were explored by using chronic constriction injury of the sciatic nerve (CCI) neuropathic pain model in vivo and LPS-induced injury in microglia BV2 cells in vitro. Immunofluorescence staining and Western blot analysis were used to assess the modulator effect of koumine on microglia and astrocyte activation after CCI surgery. …”
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The Role of SUMO1 Modification of SOX9 in Cartilage Development Stimulated by Zinc Ions in Mice by Na Xue, Jing Zhao, Jing Yin, Liang Liu, Zhong Yang, Shuchao Zhai, Xiyun Bian, Xiang Gao

“…Subsequently, we subjected the embryos to scrutiny employing HE, Safranin O staining and IHC. Primary chondrocytes were isolated from fetal mouse femoral condyles and utilized for Western blot analysis to discern the expression profiles of SUMO1, SUMO2/3, UBC9, SOX9, MMP13, Collagen II, RUNX2, and aggrecan. …”
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Mechanism of action of Xipayimaizibizi oral liquid on outlet obstruction-induced overactive bladder: An integrated study by Menglu Wang, Yang Yang, Yuhang Du, Jiamei Xie, Yige Zhao, Yongcheng An, Ziyi Shan, Shenyujun Wang, Meng Hao, Baosheng Zhao

“…Hematoxylin and eosin and Masson's trichome staining were used to observe changes in the bladder structure. …”
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Infiltration of CD8+ cytotoxic T-cells and expression of PD-1 and PD-L1 in ovarian clear cell carcinoma by Mengdi Fu, Huimei Zhou, Jiaxin Yang, Dongyan Cao, Zhen Yuan

“…The optimized multiplex immunofluorescence technique was used to stain paraffin sections for immune factors related to the immune microenvironment of OCCC and clinical prognosis. …”
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Identification of quality markers and mechanisms of Anzi Tiaochong Fang in the treatment of antiphospholipid syndrome-related recurrent pregnancy loss: chemical analysis, network p... by Shan He, Fei Ma, Jia Li, Da-yan Liu, Zhi-wei Wang, Peng-dian Chen, Jia-man Wu, Hong Chang, Yan Ning

“…In vitro verification was performed using CCK-8, FDA staining, and ELISA. Results This study identified 23 compounds and 942 targets, including 132 APS-RPL targets and 42 targets between ATF and APS-RPL. …”
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Tanshinone IIA Sodium Sulfonate Attenuates LPS-Induced Intestinal Injury in Mice by Xin-Jing Yang, Jin-Xian Qian, Yao Wei, Qiang Guo, Jun Jin, Xue Sun, Sheng-Lan Liu, Chun-Fang Xu, Guo-Xing Zhang

“…The intestinal tissue injury was analyzed by HE staining. Inflammatory factors (TNF-α, IL-1β, and IL-6) in the intestinal tissue were examined by ELISA and RT-PCR. …”
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The critical role of MLKL in hemorrhagic stroke and the therapeutic potential of its associated protein network by Yi Wang, Yi Wang, Moran Xu, Xiaoli Zuo, Sheng Wang, Yong Yu, Zhaobing Gao, Zhaobing Gao, Jingbo Qie, Ye Jiang, Fang Huang, Bingqing Xia, Bingqing Xia

“…However, the mechanisms by which MLKL functions in the process of intracerebral hemorrhage (ICH)-induced damage remain unclear.MethodsHere, we explored the correlation between MLKL and pathological damage in ICH patients through histopathological staining and RT-qPCR. Furthermore, we established an intracerebral hemorrhage model by collagenase IV injection in WT and Mlkl-/- mice. …”
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Remote photobiomodulation ameliorates behavioral and neuropathological outcomes in a rat model of repeated closed head injury by Chongyun Wu, Meng Li, Zhe Chen, Shu Feng, Qianting Deng, Rui Duan, Timon Cheng-Yi Liu, Luodan Yang

“…Behavioral deficits were assessed through three-chamber social interaction, forced swim, grip strength, open field, elevated plus maze, and Barnes maze tests. Immunofluorescence staining and 3D reconstruction evaluated neuronal damage, apoptosis, degeneration, and the morphology of microglia and astrocytes, as well as astrocyte and microglia-mediated excessive synapse elimination. …”
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Osimertinib exacerbates immune checkpoint inhibitor-related severe adverse events by activating the IL-6/JAK/STAT3 pathway in macrophages by Yuan Li, Yanping Chen, Yuan Meng, Meng Shen, Fan Yang, Xiubao Ren

“…Lung micro-CT scans were used to assess the degree of inflammatory infiltration. Hematoxylin-eosin staining was used to analyze the histopathologic inflammatory infiltration in mouse liver and lung tissues. …”
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Alleviating the IL-1β-stimulated extracellular matrix degradation in osteoarthritis, and chondrocyte inflammation by Morinda officinalis polysaccharide via the SIRT6/NF-κB pathway... by Dongfang Zhao, Shuqin Xing, Jiao Qi, Zhiqiang Wei, Jianghai Huang, Jigao Sun, Xinzhu Wen, Yafei Wang

“…Cell damage was assessed using flow cytometry and Hoechst 33258 fluorescent staining. Inflammatory factor levels were measured via western blot and ELISA, while extracellular matrix (ECM) degradation was analyzed through immunofluorescence. …”
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Role of microvascular pericyte dysfunction in antibody-mediated rejection following kidney transplantation by Jie Xu, Junyan Pu, Hao Chen, Li Sun, Shuang Fei, Zhijian Han, Jun Tao, Xiaobing Ju, Zijie Wang, Ruoyun Tan, Min Gu

“…Postoperative renal function indicators were compared, and immunohistochemical and immunofluorescence staining was performed on graft tissues and mice models using the pericyte marker PDGFR-β. …”
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Hypertensive disorders of pregnancy affected thyroid hormone synthesis via endoplasmic reticulum stress in preterm infant rats by Maomao Sun, Congrong Wu, Jie Jiang, Yue He, Sha Zhu, Yonghui Yu

“…Thyroid hormone levels of preterm infant rats were detected by ELISA, and morphology structure were observed by H&E staining and electron microscopy, and the expression of key factors of thyroid hormone synthesis and endoplasmic reticulum stress indicators were analyzed by RT-qPCR and Western blotting. …”
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Apoptotic PET Imaging of Rat Pulmonary Fibrosis With [F]ML-8 by Ying Xiong MM, Dahong Nie BM, Shaoyu Liu PhD, Hui Ma BM, Shu Su BM, Aixia Sun PhD, Jing Zhao MD, PhD, Zhanwen Zhang PhD, Xianhong Xiang MD, PhD, Ganghua Tang MD, PhD

“…Compared to control rats, BLM-treated rats had higher lung hydroxyproline content ( P < .05). Immunofluorescence staining indicated more apoptotic cells in BLM-treated rats than those in control rats. …”
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Intense Pulsed Light Therapy with Optimal Pulse Technology as an Adjunct Therapy for Moderate to Severe Blepharitis-Associated Keratoconjunctivitis by Fang Ruan, Yunxiao Zang, Ruti Sella, Hongshuang Lu, Shang Li, Ke Yang, Tao Jin, Natalie A. Afshari, Zhiqiang Pan, Ying Jie

“…Efficacy outcome measures included meibum quality, Meibomian gland (MG) secretion function, eyelid margin signs, corneal fluorescein staining (CFS) score, noninvasive keratography breakup time (NIKBUT), ocular surface disease index (OSDI) score, Schirmer I test (SIT), classification of tear film lipid layer (TFLL), and Meibomian gland dropout (MGDR). …”
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Total flavonoids isolated from Eucommia ulmoides can alleviate bone loss and regulate intestinal microbiota in ovariectomized rats by Baocang Yin, Baocang Yin, Mingzhen Yang, Mingzhen Yang, Bowen Wang, Bowen Wang, Yun Zhang, Yun Zhang, Ningli Li, Ningli Li, Qin Li, Yingying Li, Cory J. Xian, Tiejun Li, Tiejun Li, Yuankun Zhai, Yuankun Zhai

“…The femoral microstructure parameters of the TFEL-M and TFEL-H groups were significantly improved compared with the OVX group, which were also confirmed by H&amp;E histological staining. High-throughput sequencing indicated that TFEL may regulate the composition of intestinal flora and intestinal microecology.ConclusionTFEL can prevent osteoporosis in OVX rats and has no toxic side effects. …”
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A nucleoside-modified mRNA vaccine prevents enterovirus A71 infection in mouse model by Fengyu Chi, Fengyu Chi, Xu Zhang, Xu Zhang, Dong Zhang, Dong Zhang, Airu Zhu, Zhen Zhuang, Zhaoyong Zhang, Zhenjie Zhang, Chuansong Quan, Kaixiao Nie, Juan Li, Chunhong Yin, Jie Tong, Yuming Li, Yuming Li, Yuming Li

“…Immune responses were assessed by ELISA, micro-neutralization assays, ELISpot, and intracellular cytokine staining (ICS). Passive protection was tested by transferring immune sera to neonatal mice challenged with EV-A71.ResultsThe VP1 mRNA-LNP vaccine elicited robust humoral and cellular immunity, including high levels of VP1-specific IgG, neutralizing antibodies, and a Th1-biased T-cell response. …”
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Discovery and in vitro characterization of a human anti-CD36 scFv by Cecilia Mata-Cruz, Cecilia Mata-Cruz, Sandra L. Guerrero-Rodríguez, Keyla Gómez-Castellano, Gregorio Carballo-Uicab, Juan Carlos Almagro, Juan Carlos Almagro, S. Mayra Pérez-Tapia, S. Mayra Pérez-Tapia, S. Mayra Pérez-Tapia, Marco A. Velasco-Velázquez

“…The phenotypic changes induced by CD36 ligands were assessed in vitro by: i) oil red staining, ii) tumorsphere assays, and iii) RT-qPCR.ResultsWe identified an anti-CD36 scFv, called D11, that competes with a commercial anti-CD36 antibody with proven efficacy in disease models. …”
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