Assessment of Myometrial Concentrations of Oestrogen and Progesterone Receptors in the Lower Uterine Segment of Full-Term Pregnancies in Presence or Absence of Labour by Joana Soares de Arruda, Edward Araujo Júnior, Manuel de Jesus Simões, Luiz Kulay Júnior

“…The biopsy of myometrium was realized during caesarian section, and the excised tissue was stained using immunohistochemical techniques for the quantification of the receptors, and with the aid of image-analysis software, the numbers of receptors for each hormone were determined spectrophotometrically. …”
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Effect of decitabine on PD-L2 methylation in whole blood of iodine-induced autoimmune thyroiditis rats by Baiming Jin, Yanbo Qi, Hong Chao, Xiaolei Yang, Hongjie Li, Siyuan Wan

“…After injecting Dec intraperitoneally into EAT rats, we performed arsenic-cerium catalytic spectrophotometry, pathological hematoxylin and eosin staining, enzyme-linked immunosorbent assay, quantitative methylation-specific polymerase chain reaction (qMSP), and quantitative real-time polymerase chain reaction (qPCR) to determine the relevant indices. …”
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Zinc Supplementation Mitigates High Salt Diet-Induced Bone Damage: A Histological Evaluation of Osteocyte Apoptosis by Kaukab Anjum, Abdullah Qamar, Nomana Mahmood, Muhammad Marghoob Khan, Shazia Imran, Ayesha Ali

“…After eight weeks, femurs were harvested, processed, and stained with hematoxylin and eosin. Osteocyte apoptosis was assessed by counting empty lacunae and apoptotic bodies under light microscopy. …”
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LncRNA GAS5 reduces blood glucose levels and alleviates renal fibrosis in diabetic nephropathy by regulating the miR-542-3p/ERBB4 axis by Qinghua Yin, Na Guo, Ruoxi Liao

“…HE, TUNEL and Masson stainings were employed to observe the cellular morphology, apoptosis, and fibrosis of renal tissues, respectively. …”
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Bi-Hormonal Endocrine Cell Presence Within the Islets of Langerhans of the Human Pancreas Throughout Life by Jiwon Hahm, Dawn Kumar, Juan Andres Fernandez Andrade, Edith Arany, David J. Hill

“…The most abundant bi-hormonal cell phenotype observed co-stained for glucagon and insulin, representing 3.1 ± 0.3% of all islet cells. …”
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Development and evaluation of a novel E7 multi-epitopic vaccine for human papillomavirus type 16: design, expression, purification, and immunological characterization by Bahareh Bahmani, Zahra Amini-bayat, Mohammad Mehdi Ranjbar, Masoud Hassanzadeh Makoui, Amir-Hassan Zarnani

“…Polyclonal antibodies were generated, and their reactivity with immunizing antigen and native protein in E7 expressing cells (TC-1) was assessed by ELISA and immunofluorescent staining, respectively. The efficacy of the vaccine was assessed in a therapeutic animal model of HPV-induced cancer. …”
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Single-cell RNA-seq reveals immune cell heterogeneity and increased Th17 cells in human fibrotic skin diseases by Cheng-Cheng Deng, Xue-Yan Xu, Yan Zhang, Long-Can Liu, Xuan Wang, Jun-Yi Chen, Liu-Yi Yao, Ding-Heng Zhu, Bin Yang

“…Single-cell RNA sequencing was used to analyze the cellular landscape of immune cells in keloid and normal scar tissues. Ki-67 staining, a scratch experiment, real-time PCR, and Western blotting were used to explore the effect of the Th17 cell supernatant on keloid fibroblasts.ResultsOur findings revealed the intricate cellular landscape of immune cells in fibrotic skin diseases. …”
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Bacterial isolates and drug susceptibility patterns in infected lesions of cutaneous leishmaniasis patients at ALERT hospital, Addis Ababa, Ethiopia by Gizealew Ayalew, Asegedech Asmamaw, Mistire Wolde, Regasa Diriba, Biruk Zerfu

“…The isolates were identified by gram staining, colony morphology, and biochemical testing. …”
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Synthesis and protective effect of pyrazole conjugated imidazo[1,2-a]pyrazine derivatives against acute lung injury in sepsis rats via attenuation of NF-κB, oxidative stress, and a... by Zang Binbin, Wang Lihui

“…Results suggested that compound 3h significantly reduced lung inflammation and membrane permeability, as evidenced by H&E staining of lung tissues. It substantially reduced the generation of pro-inflammatory cytokines (TNF-α, IL-1B, IL-6) and oxidative stress (MPO, MDA, SOD). …”
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Extracellular MMP-9-Based Assessment of Ocular Surface Inflammation in Patients with Primary Open-Angle Glaucoma by Anna Zaleska-Żmijewska, Ewa Strzemecka, Zbigniew M. Wawrzyniak, Jacek P. Szaflik

“…Further assessments of disease severity and inflammation comprised Goldmann applanation tonometry for intraocular pressure (IOP), Schirmer’s test with anesthesia, ocular surface staining with unpreserved fluorescein (Oxford scale index), tear breakup time (TBUT), McMonnies questionnaire, and the Ocular Surface Disease index (OSDI). …”
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Human Platelets Take up Anti-VEGF Agents by B. Sobolewska, B. Fehrenbacher, P. Münzer, H. Kalbacher, S. Geue, Konstantinos Stellos, M. Schaller, F. Ziemssen

“…The uptake of anti-VEGF agents with or without VEGF treatment was investigated using immunofluorescence and immunogold staining in human platelets. The role of actin filaments and clathrin-coated vesicles in the transport of ranibizumab, aflibercept, and bevacizumab was evaluated by two pharmacologic inhibitors: staurosporine (protein kinase C inhibitor) and cytochalasin D. …”
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Pathological findings in rainbow trout (oncorhynchus mykiss, walbaum 1792) in experimental vagococcus salmoninarum infection by S. Serap Birincioğlu, Hamdi Avcı, T. Tansel Tanrikulu, E.Tuğrul Epikmen, M. Lütfi Avsever

“…For histopathological examinations, all tissue samples were fixed and than fixation samples routinely were processed. Tissue sections stained with haematoxylin-eosin and examined using light microscope. …”
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Downregulation of VRK1 Inhibits Progression of Lung Squamous Cell Carcinoma through DNA Damage by Ning Du, Boxiang Zhang, Yunfeng Zhang

“…The effect of VRK1 downregulation on DNA damage response (DDR) was examined by immunofluorescence staining and western blot assays and further validated by in vivo experiments. …”
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Comparison of upper eyelid skin thickness at 5 mm and 12 mm from the eyelid margin in Asian by Hirohiko Kakizaki, Chrisha Habaluyas, Osama AlSheikh, Munekazu Naito, Yasuhiro Takahashi

“…The specimens were fixed in 10% formalin and stained with Masson’s trichrome. Skin thickness was measured at 5 and 12 mm from the eyelid margin and compared. …”
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Human endometrial stem cell-derived small extracellular vesicles enhance neurite outgrowth and peripheral nerve regeneration through activating the PI3K/AKT signaling pathway by Mojdeh Salehi Namini, Nima Beheshtizadeh, Somayeh Ebrahimi-Barough, Jafar Ai

“…For this purpose, hEnSC-derived small EVs were extracted by ultracentrifuge and characterized by DLS, SEM, TEM, and western blot. Also, dil-staining of hEnSC-derived small EVs was done to determine the penetration of hEnSC-derived small EVs into PC12 cells. …”
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Synergetic role of TRPV4 inhibitor and mechanical loading on reducing inflammation by Parto Babaniamansour, Diego Jacho, Agustin Rabino, Rafael Garcia-Mata, Eda Yildirim-Ayan

“…Surface protein analysis via immunohistochemistry confirmed these phenotypic shifts, highlighting changes in the expression of CD80 (pro-inflammatory) and CD206 (anti-inflammatory) markers, alongside F-actin and nuclear staining. This research suggests that TRPV4 inhibition, combined with specific mechanical loading (6%), can drive macrophages toward an anti-inflammatory state, thereby may promote inflammation resolution and tissue repair.…”
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Methicillin-Resistant Staphylococcus aureus: The Magnitude and Risk Factors among Patients Admitted to Tikur Anbessa Specialized Hospital, Addis Ababa, Ethiopia by Tewodros Tamire, Temesgen Eticha, Temesgen Bati Gelgelu

“…Clinical specimens of pus and blood were collected from admitted patients who developed the infection after 48 hours of admission. Gram stain, culture media preparations, and biochemical tests were conducted to identify and isolate the causative agent. …”
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Identification and Clinical Validation of High HSP60 Expression Predicts Poor Prognosis in Patients with Ovarian Cancer by Zheng M, Li S, Deng J, Huang C, Zhang H

“…Patients were categorized into high- or low-HSP60 expression groups based on immunohistochemical staining intensity. The correlation between HSP60 expression status and the clinicopathological features of ovarian cancer patients was analyzed. …”
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Retrospective Analysis of Ventriculitis in External Ventricular Drains by Stephen Albano, Blake Berman, Glenn Fischberg, Javed Siddiqi, Bolin Liu, Yasir Khan, Atif Zafar, Syed A. Quadri, Mudassir Farooqui

“…There were 2 cases with a CSF having a positive gram stain but failed to yield any bacterial growth on culture and did not meet predefined criteria. …”
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The Process of Acclimation to Chronic Hypoxia Leads to Submandibular Gland and Periodontal Alterations: An Insight on the Role of Inflammatory Mediators by Antonela Romina Terrizzi, María Inés Conti, María Pilar Martínez, Javier Fernández-Solari

“…Only the CCH group showed higher HIF-1α staining, while CIH alone exhibited interradicular bone loss and increased concentration of the bone resorption marker CTX-I. …”
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