Evidence for Decreased Nucleolar PARP-1 as an Early Marker of Cognitive Impairment by Matthew Regier, Jiancong Liang, Alexander Choi, Kavita Verma, Jenny Libien, A. Iván Hernández

“…We found a significant decrease of PARP-1 staining in the nucleolar compartment of hippocampal pyramidal cells in MCI compared with Control and AD. …”
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Metformin and Probiotics Interplay in Amelioration of Ethanol-Induced Oxidative Stress and Inflammatory Response in an In Vitro and In Vivo Model of Hepatic Injury by Farhin Patel, Kirti Parwani, Dhara Patel, Palash Mandal

“…Endoplasmic reticulum (ER) stress markers, inflammatory markers, lipid metabolism, reactive oxygen species (ROS) production, and oxidative stress were evaluated, using qRT-PCR, Oil red O staining, fluorimetry, and HPLC. In vitro, probiotic V and Met in combination prevented ethanol-induced cellular injury, ER stress, oxidative stress, and regulated lipid metabolism as well as inflammatory response in HepG2 cells. …”
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The Result of Timely Therapy for Whipple's Disease: a Clinical Case by S. N. Mekhtiev, O. A. Mekhtieva, E. Yu. Kalinina, V. E. Karev, A. V. Vanyan, O. M. Berko

“…The identified changes were represented by thickening of the villi and accumulations in the stroma of large macrophages (CD68+) with wide light cytoplasm containing abundant accumulations of PAS-positive, negative when stained with carbol fuchsin according to Ziehl — Nielsen and auramine-rhodamine (under microscopy in luminescence mode) short rods, as well as numerous optically empty small and larger cavities. …”
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Effects of Insulin-like Growth Factor 1 on the Maintenance of Cell Viability and Osteogenic Differentiation of Gingiva-Derived Mesenchymal Stem Cell Spheroids by Somyeong Hwa, Hyun-Jin Lee, Youngkyung Ko, Jun-Beom Park

“…However, on day 7, the incorporation of IGF-1 led to an increase in Alizarin Red staining, indicative of enhanced calcium deposition. …”
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Deguelin inhibits the proliferation of human multiple myeloma cells by inducing apoptosis and G2/M cell cycle arrest: Involvement of Akt and p38 MAPK signalling pathway by Sun Kening, Chen Ping, Zhang Liang, Lu Zhidong, Jin Qunhua

“…The apoptosis of multiple myeloma cells was studied using AO/EB and Annexin V-FITC/PI staining methods. Multiple myeloma cell cycle analysis was performed through flow cytometry. mRNA expression levels were depicted using qRT-PCR. …”
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Dry eye post-cataract surgery: a systematic review and meta-analysis by Hillary Ta, Paul McCann, Mengli Xiao, Tiffany Lien, Kaleb Abbott, Darren G. Gregory, Riaz Qureshi, Tianjing Li

“…We compared postoperative dry eye outcomes with baseline including Ocular Surface Disease Index (OSDI), tear break up time (TBUT), Schirmer’s I test (ST1), and corneal fluorescein staining (CFS) at short-term (< 1 week) and medium-term (≥ 1 week to 3 months) follow-up. …”
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Morphometric, histometric and histomorphological investigation of the effects of different ratios safflower (chartamus tinctorius l.) oil supplements on glandula uropygialis in jap... by İsmail Demircioğlu, Bestami Yılmaz, Kürşat Filikçi, Gülşah Güngören, Faruk Bozkaya

“…Routine tissue processing was performed for histometric examination, then the tissues were stained with Haematoxylin-Eosin and Sudan Black.<p> <b>Results:</b> The results indicated that safflower oil supplementatio had a significant effect on papillary length, left lobe length, glandular weight, relative glandular weight, glycogen region thickness(p<0.05) and right lobe length with (p<0.01). …”
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Tissue-friendly dentin treatments as a potential element in revascularization protocol (ex-vivo study) by Hala Fayek Khalil, Nadia Ezz El Din Metwalli, Sara Magdy, Mohamed Shamel

“…Osteogenic differentiation was assessed through alizarin red staining for calcium deposition and quantitative PCR analysis of BMP-2, TGF-β1, VEGF, and DSPP gene expression. …”
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Key wound healing genes as diagnostic biomarkers and therapeutic targets in uterine corpus endometrial carcinoma: an integrated in silico and in vitro study by Fuchuan Jiang, Sajjad Ahmad, Sadia kanwal, Yasir Hameed, Qian Tang

“…Validation using TCGA datasets revealed consistent downregulation of these genes in UCEC samples, corroborated by immunohistochemical staining. Promoter methylation analysis showed significantly higher methylation levels in UCEC, correlating with decreased mRNA expression and poor survival outcomes. …”
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Molecular Detection and Multidrug Resistance of Shigella spp. Isolated from Wild Waterfowl and Migratory Birds in Bangladesh by Jarna Karmoker, Md. Saiful Islam, Md. Liton Rana, Md. Ashek Ullah, Fahim Haque Neloy, Nahian Muniath Oishy, Pritom Kumar Pramanik, Mahbubul Pratik Siddique, Sukumar Saha, Md. Tanvir Rahman

“…Shigella spp. were isolated and identified by culturing, staining, and biochemical tests followed by polymerase chain reaction (PCR). …”
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Second harmonic generation imaging reveals entanglement of collagen fibers in the elephant trunk skin dermis by Andrew K. Schulz, Magdalena Plotczyk, Sophia Sordilla, David C. A. Gaboriau, Madeline Boyle, Krishma Singal, Joy S. Reidenberg, David L. Hu, Claire A. Higgins

“…To investigate how this is achieved, we used classical histochemical staining and second harmonic generation microscopy to describe the morphology and composition of elephant trunk skin. …”
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In Vitro Induction of Hypertrophic Chondrocyte Differentiation of Naïve MSCs by Strain by Thomas Jörimann, Priscilla Füllemann, Anita Jose, Romano Matthys, Esther Wehrle, Martin J. Stoddart, Sophie Verrier

“…Mechanical deformation alone led to a larger cell size and a more rounded cell morphology characteristic of hypertrophic chondrocytes, while lower GAG and proteoglycan production was observed. Immunohistology staining corroborated the gene expression data, showing increased Type X collagen with mechanical strain. …”
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Evaluation of the Effect of Different Glazing Brands on Surface Roughness of Monolithic Zirconia by Riyam Imad Fouad, Lateef Essa Alwan Al Jorani, Abdel Kareem Jasim Khadim Al-Azzawi

“…The samples dimension for the surface roughness test (diameter 10 mm and thickness 2 mm) by using a surface roughness testing machine profilometer (TR200, Germany), divided into 3 groups depending on types of glazing brands materials (VITA, VITA AKZENT® Plus, glaze LT, Germany), the second group with (Ivoclar Vivadent, IPS e.max Ceram glaze and stain liquid, Liechtenstein), and the third group with (GC Initial spectrum glaze liquid, Austria). …”
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Explore Alteration of Lung and Gut Microbiota in a Murine Model of OVA-Induced Asthma Treated by CpG Oligodeoxynucleotides by Wang Q, Ji J, Xiao S, Wang J, Yan X, Fang L

“…Qingqing Wang, Jingjing Ji, Shuaijun Xiao, Jiong Wang, Xuebo Yan, Lei Fang Department of Geriatric Respiratory and Critical Care, The First Affiliated Hospital of Anhui Medical University, Anhui Geriatric Institute, Hefei, Anhui, People’s Republic of ChinaCorrespondence: Jiong Wang, Email wangjiong7286@163.comAim: We sought to investigate the impact of CpG oligodeoxynucleotides (CpG-ODN) administration on the lung and gut microbiota in asthmatic mice, specifically focusing on changes in composition, diversity, and abundance, and to elucidate the microbial mechanisms underlying the therapeutic effects of CpG-ODN and identify potential beneficial bacteria indicative of its efficacy.Methods: HE staining were used to analyze inflammation in lung, colon and small intestine tissues. …”
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A mouse model of immunosuppression facilitates oral Candida albicans biofilms, bacterial dysbiosis and dissemination of infection by Raja Veerapandian, Anuja Paudyal, Sarah M. Schneider, Sonny T. M. Lee, Govindsamy Vediyappan, Govindsamy Vediyappan

“…Results showed abundant growth of resident oral bacteria and a strong C. albicans biofilm on the tongue consisting of hyphae which damaged papillae, the epidermal layer, and invaded tongue tissue with the accumulation of inflammatory cells as demonstrated by Grocott’s methenamine silver and hematoxylin and eosin staining, respectively. The dispersed microbes from the oral biofilm colonized the gastrointestinal (GI) tract and damaged its integrity, disseminating microbes to other organs. …”
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Patient‐Reported Fatigue Associated with Joint Histopathology in Rheumatoid Arthritis by Diyu Pearce‐Fisher, Melanie H. Smith, Bella Y. Mehta, Edoardo Spolaore, Edward DiCarlo, Dongmei Sun, Susan M. Goodman

“…Hematoxylin and eosin stains of sectioned synovium were systematically scored by a pathologist. …”
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Using β-Elemene to reduce stemness and drug resistance in osteosarcoma: A focus on the AKT/FOXO1 signaling pathway and immune modulation by Shaochun Zhang, Zhijie Xing, Jing Ke

“…Bioinformatics analysis, flow cytometry, and immunofluorescence staining were used to evaluate signaling pathway activity and macrophage polarization. …”
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Dissecting macrophage heterogeneity and kaempferol in lung adenocarcinoma: a single-cell transcriptomic approach and network pharmacology by Laiyi Wan, Wentao Hao, Leilei Li, Lin Wang, Yanzheng Song

“…Pseudotime trajectory analysis, immunofluorescence staining, and in vitro assays examined the functional roles of TAMs and the effects of selected compounds on macrophage polarization. …”
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Chemical Composition and Biological Activities of the Essential Oils of Chrysophyllum albidum G. Don (African Star Apple) by Daniel Nartey, Joseph Nana Gyesi, Lawrence Sheringham Borquaye

“…The antibiofilm activity was assessed via the crystal violet staining assay, with Pseudomonas aeruginosa as the model organism. …”
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The Mutual Inhibition of FoxO1 and SREBP-1c Regulated the Progression of Hepatoblastoma by Regulating Fatty Acid Metabolism by Yu Hu, Hongyan Zai, Wei Jiang, Zhenglin Ou, Yuanbing Yao, Qin Zhu

“…The morphology of tumor tissue sections was observed by HE staining. Results. FoxO1 expression was downregulated in HB tissue, while the expressions of SREBP-1c, FASN, ACLY, ACC, and MAGL were upregulated. …”
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