Therapeutic effects of resveratrol and β-carotene on L-arginine-induced acute pancreatitis through oxidative stress and inflammatory pathways in rats by Arzu Gezer, Hilal Üstündağ, Mustafa Özkaraca, Ebru Karadağ Sari, Cihan Gür

“…Immunohistochemical (IHC) staining with anti-insulin antibody revealed reduced β + langerhans islet size in the AP group. qPCR analysis indicated significant upregulation of inflammatory genes NF-κB, TNF-α, and IL-1β (p < 0.001), and apoptotic genes Bax and Caspase-3, with downregulation of Bcl-2 (p < 0.001). …”
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Pengaruh Hiperglikemia terhadap Gambaran Histopatologis Glomerulus Mencit (Mus musculus Linn) yang Diinduksi Aloksan by Melati Setia Ningsih, Eryati Darwin, Erlina Rustam

“…The tissues were processed by paraffin embedding to obtain histopathological sections and stained with haematoxylin-eosine. Morphometric analysis of glomerulus showed that the glomerulus area, circumference and diameter were increased in group P (p&lt;0.05).The result suggest that hyperglycemia induced by alloxan for 14 days caused significant changes in histopathological features of mice glomerulus.…”
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Zebularine Promotes Hepatic Differentiation of Rabbit Bone Marrow Mesenchymal Stem Cells by Interfering with p38 MAPK Signaling by Yong-Heng Luo, Juan Chen, En-Hua Xiao, Qiu-Yun Li, Yong-Mei Luo

“…BMMSCs were treated with zebularine at concentrations of 10, 20, 50, and 100 μM in the presence of hepatocyte growth factor; changes in the levels of hepatic-specific alpha-fetoprotein and albumin were detected and determined by RT-PCR, WB, and immunofluorescence staining. Expression of DNMT1 and phosphorylated p38 as well as urea production and ICG metabolism was also analyzed. …”
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Classifying tumour infiltrating lymphocytes in oral squamous cell carcinoma histopathology using joint learning framework by Barun Barua, Genevieve Chyrmang, Kangkana Bora, Gazi N. Ahmed, Lopamudra Kakoti, Manob Jyoti Saikia

“…Our model employs two parallel encoders: one extracts cellular features from cellular density maps, while the other processes tissue features from H&E-stained tissue images. This dual-encoder approach enables OralTILs-ViT to capture complex tissue-cellular interactions, classifying TILs infiltration categories consistent with Broders’ grading system-“Moderate to Marked”, “Slight” and “None to Very Less.” …”
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An Evaluation of the Antibacterial Properties of Tormentic Acid Congener and Extracts From Callistemon viminalis on Selected ESKAPE Pathogens and Effects on Biofilm Formation by Tafadzwa Chipenzi, Genuine Baloyi, Tatenda Mudondo, Simbarashe Sithole, Godloves Fru Chi, Stanley Mukanganyama

“…Biofilm formation and detachment assays were performed using crystal violet staining. Production of extracellular polymeric DNA and polysaccharides from biofilms was also determined. …”
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Sub-toxic cisplatin concentrations induce extensive chromosomal, nuclear and nucleolar abnormalities associated with high malignancy before acquired resistance develops: Implicatio... by John G Delinassios, Robert M Hoffman, George Koumakis, Dimitrios Palitskaris, Kyriaki-Nefelli Poulatsidou, George J Delinasios

“…Cells treated with 0.1 and 0.2 μg/ml, termed HeLaC0.1 and HeLaC0.2, were not cisplatin-resistant, only exhibiting a slightly reduced viability, and were termed "cisplatin-sensitized cells." Giemsa and silver staining were used to detect nuclear and nucleolar abnormalities and chromosomal alterations.…”
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Mechanistic insights into epithelial-mesenchymal transition mediated cisplatin resistance in ovarian cancer by Taha Umair Wani, Hyun-Yi Kim, Geum-Hwa Lee, Young Je Lim, Han-Jung Chae, Ji-Ye Kim, Hyonok Yoon

“…Finally, the immunohistochemical staining confirmed the high protein expression in resistant tumor.…”
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Qingfei Tongluo Mixture Attenuates Bleomycin-Induced Pulmonary Inflammation and Fibrosis through mTOR-Dependent Autophagy in Rats by Shuyu Ge, Zhenghong Guo, Ting Xiao, Pingping Sun, Bo Yang, Yin Ying

“…Body weight and lung coefficient were examined, combined with hematoxylin and eosin (H&E) and Masson staining to observe lung tissue lesions. The enzyme-linked immunosorbent assay (ELISA) and the hydroxyproline (HYP) assay kit were used to detect changes in proinflammatory factors (transforming growth factor-β (TGF-β), tumor necrosis factor-α (TNF-α), and interleukin-1β (IL-1β)) and HYP. …”
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Establishment of an oral burn model in streptozotocin-induced diabetic rats by Su-Young Kim, Seong-Gon Kim, Dae-Won Kim, Ji-Hyeon Oh

“…At 1- and 2-weeks post-surgery, macroscopic examination, histological staining, immunohistochemistry, and Western blot analysis were performed to compare the healing progress. …”
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Association between cytokines and progression-free survival in ovarian cancer following CRS/HIPEC treatment by Chao-Yu Chen, Ting-Yao Wang, Jing-Lan Liu, Yu-Che Ou, Li-Wen Lee, Chien-Hui Hung, Chuan-Pin Lee, Jrhau Lung

“…Immunohistochemical (IHC) staining was performed on tissue microarrays from 19 patients. …”
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Increasing Donor Endothelial Cell Pool by Culturing Cells from Discarded Pieces of Human Donor Corneas for Regenerative Treatments by Mohit Parekh, Vito Romano, Alessandro Ruzza, Stephen B. Kaye, Diego Ponzin, Sajjad Ahmad, Stefano Ferrari

“…Donor corneas (n = 19) with a mean age of 72 years, male : female ratio of 15 : 4, and death-to-preservation time of 10 hours were assessed for endothelial cell density (ECD) and number of dead cells before isolation. Alizarin red staining (n = 3) was performed to check the morphology of cells in the center and periphery. …”
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Phenotypic and genotypic screening of multidrug resistant Klebsiella pneumoniae isolated from ready to eat street food in Tanta, Egypt by Hadeer M. Bedair, Mohamed Emara, Shima Mahmoud Ali, Tamer M. Samir, Mahmoud A.F. Khalil

“…Klebsiella pneumoniae isolates were identified biochemically and by Gram and capsular staining then isolates were assessed for antimicrobial resistance phenotypically. …”
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Reversible Ca2+ signaling and enhanced paracellular transport in endothelial monolayer induced by acoustic bubbles and targeted microbeads by Jiawei Lin, Chaofeng Qiao, Hao Jiang, Zhihui Liu, Yaxin Hu, Wei Liu, Yu Yong, Fenfang Li

“…Further immunofluorescence staining showed disruption of cell junctions with microbubble stimulation and reversible remodeling of many cell junctions by ultrasound with integrin-targeted microbeads. …”
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Application of PNR Detection in the Diagnosis and Drug-efficacy Evaluation of Diabetic Kidney Disease in Rats by ZHANG Naiqun, YUAN Piaopiao, CAO Linrong, YING Na, YANG Taotao

“…Hematoxylin and eosin staining (HE) was used to observe glomerular structure, while transmission electron microscopy examined the ultrastructure of glomerular podocytes.ResultsPNR reached the critical value(≥1) on day 9 after modeling, earlier than U-ACR, which reached the critical value(≥30 mg/g) on day 15. …”
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Innovative surgical and stress-stimulated rat model of ligamentum flavum hypertrophy by Long Chen, Zhaoyuan Zhang, Niandong Li, Wanxia Zhang, Zhouhang Zheng, Yu Zhang

“…Seven weeks later, MRI, immunohistochemistry (IHC), and western blot (WB) will be performed on the LF of the L5-6 segment in the two groups of rats.ResultsMRI results showed that the LF in the experimental group was significantly thicker than that in the sham group. Masson staining results indicated that LF thickness, collagen fiber area, and collagen volume fraction (CVF) were significantly higher in the experimental group than in the sham group. …”
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Treatment Effect of a Vascular-Disrupting Agent Dynamically Monitored by DWI: An Animal Experimental Study by Danping Huang, Ruimeng Yang, Yong Zou, Hongmei Lin, Xiangdong Xu, Xinhua Wei, Hanzheng Chang, Liqiong Wu, Wenshuang Ding, Wenjie Tang, Xinqing Jiang

“…Two randomly chosen rabbits from each group were sacrificed for H&E staining and CD34 immunohistochemical assessments at each time point. …”
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Comparison of Nutritional and Meat Quality Characteristics between Two Primal Cuts from Aceh Cattle in Aceh Province, Indonesia by Hamny Sofyan, Aryani Sismin Satyaningtijas, Cece Sumantri, Etih Sudarnika, Srihadi Agungpriyono

“…Muscle microstructure analysis was performed using the HE, Masson’s trichrome, and immunohistochemistry staining methods, while the MSTN gene expression analysis was performed using the qPCR method. …”
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Pancreas Proapoptotic Proteases in Patients with Chronic Pancreatitis by N. B. Gubegritz, E. A. Krylova, Yu. A. Gaidar

“…Microscopic tissue sections were prepared and subsequently stained with hematoxylin-eosin and by Mallory-Slinchenko. …”
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Predictive Value of Netrin-1 Expression and Ultrasonic Blood Flow in Cervical Intraepithelial Neoplasia Severity by Liu B, Wei L, Wu L, Wang H, Wang H, Yu Q

“…The expression levels of Netrin-1 were evaluated through immunohistochemical staining and quantitative fluorescence PCR. Doppler ultrasound was employed to quantify flow index (FI), vascularization index (VI), and vascularization flow index (VFI) using VOCAL software. …”
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Correlation between intestinal stem cell niche changes and small bowel crypt failure in patients with treatment-naïve celiac disease by Prasenjit Das, Sudha Battu, Lalita Mehra, Alka Singh, Muzaffar Ahmad, Ashish Agarwal, Ashish Chauhan, Anam Ahmad, Sreenivas Vishnubhatla, Siddhartha Datta Gupta, Vineet Ahuja, Govind Makharia

“…We also analyzed IgA anti-tTG2 antibody deposits by using dual-color immunofluorescence staining. Results: We found that in biopsies from patients with treatment-naive CeD with modified Marsh grade 3a–3c changes, the epithelial H2AX apoptotic index was upregulated than in controls. …”
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