Mast Cell Activation, Neuroinflammation, and Tight Junction Protein Derangement in Acute Traumatic Brain Injury by Duraisamy Kempuraj, Mohammad Ejaz Ahmed, Govindhasamy Pushpavathi Selvakumar, Ramasamy Thangavel, Sudhanshu P. Raikwar, Smita A. Zaheer, Shankar S. Iyer, Casey Burton, Donald James, Asgar Zaheer

“…We analyzed mast cell activation by toluidine blue staining, serum chemokine C-C motif ligand 2 (CCL2) level by enzyme-linked immunosorbent assay (ELISA), and proteinase-activated receptor-2 (PAR-2), a mast cell and inflammation-associated protein, vascular endothelial growth factor receptor 2 (VEGFR2), and blood-brain barrier tight junction-associated claudin 5 and Zonula occludens-1 (ZO-1) protein expression in the brains of TBI mice. …”
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Slide Malaria Positif pada Murid SDN 45 Pulau Karam Kecamatan Koto XI Tarusan Kabupaten Pesisir Selatan by Fitria Ramanda, Nurhayati , Endrinaldi

“…Data obtained by microscopic examination of thick and thin blood preparations were daubed with Giemsa staining. The study of 97 students of SDN 45 Pulau Karam in getting the numbers as much as 28% and the Plasmodium species is found throughout Plasmodium falciparum. …”
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STAT3 Expression and Its Correlation with PD-L1 Expression in Non-Hodgkin’s Lymphoma and Hodgkin’s Lymphoma at Dr. Saiful Anwar Regional Public Hospital in Malang, Indonesian Popul... by Ailen Oktaviana Hambalie, Eviana Norahmawati, Agustina Tri Endharti, Diah Prabawati Retnani, Nayla Rahmadiani

“…Samples were obtained from paraffin blocks of patients with confirmed diagnoses of NHL and HL, and then immunohistochemical staining was carried out with PD-L1 and STAT3 antibodies. …”
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KLF5 silencing restrains proliferation, invasion, migration and angiogenesis of gallbladder carcinoma cells by transcriptional regulation of PDGFA by Xiaowei Lu, Kui Hu, Dandan Zhang, Xuefeng Yin, Jifeng Nie, Kai Zhao

“…Cell proliferation was assessed utilizing the Cell Counting Kit-8 assay and EDU staining. Cell apoptosis was evaluated using flow cytometry, and apoptosis-related proteins was examined by western blotting. …”
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Salubrinal Regulates the Apoptosis of Adrenocortical Carcinoma Cells via the PERK/eIF2α/ATF4 Signaling Pathway by Lili Wu, Chunfeng Liang, Xuemei Huang, Xiujun Deng, Jiming Jiang, Zuojie Luo

“…The ACC cell lines, including SW-13 and NCI–H295 R, were used. 3-(4,5)-Dimethylthiazol(-z-y1)-3,5-di-phenytetrazoliumromide (MTT) assay, cell scratch experiments, flow cytometry, and JC-1 staining assays were performed to detect the cell viability, cell migration, and cell apoptosis. …”
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Comparative evaluation of localized genotoxicity between the field of focus and scatter following exposure to cone beam computed tomography using buccal mucosal micronuclei assay:... by Rajeswari Sreeenivas, Algi Nadil, Priya Thomas, Giju George Baby, Deepu George Mathew, Pooja Latti, Jiss George

“…Before and after CBCT exposure, samples of exfoliated oral mucosa were stained with Feulgen. Nuclear anomalies were then graded using image analysis software, and 500 intact exfoliated epithelial cells were checked for micronuclei and other nuclear anomalies according to 1992 criteria set by Tolbert et al. …”
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Efficacy of Osthole in Management of Hypoperfused Retina by Ran Du, Zhao-yang Meng, Jia-lin Wang, Yan-ling Wang

“…Histological analysis of retinal tissue revealed an increase in retinal thickness in all treatment groups, and significant improvement was noticed in the osthole-IG group. TUNEL staining revealed fewer apoptotic cells in the osthole-IG and osthole-PI groups than in the other groups. …”
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Correlation of Grade of Urothelial Cell Carcinomas and DNA Histogram Features Assessed by Flow Cytometry and Automated Image Cytometry by Marco G. W. Bol, Jan P. A. Baak, Bianca v. Diermen, E. A. M. Janssen, Susanne B. K. Buhr-Wildhagen, Kjell-Henning Kjellevold

“…Single cell suspensions were stained (DAPI for FCM, Feulgen for ICM). There was enough material for both FCMand ICMin 202 of these cases. …”
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Investigating the Effect of 8 Weeks of Endurance Training on the Levels of Inflammatory Markers (IL-6/TNF-α) and Some Metabolic Parameters in Male Rats with Nonalcoholic Fatty Live... by Vahid Kazemizadeh, Amirabbas Monazzami, Fakhredin Saba, Andrew J McAinch

“…After 17 weeks, two rats from each group were sacrificed, and liver tissue was collected for hematoxylin-eosin staining. Subsequently, the remaining 20 rats in the (I) group were divided into a training group (T) and a control group (C). …”
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Characterization of infectious salmon anaemia virus survival and infectivity in seawater by Simon Chioma Weli, Marit Måsøy Amundsen, Ole Bendik Dale, Sonal Jayesh Patel

“…In addition, the intensity of positive IFAT staining of cells infected with the eluted spiked seawater was proportional to the viral load obtained by RT-qPCR. …”
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Breast Implant Illness May Be Rooted in Mast Cell Activation: A Case-Controlled Retrospective Analysis by Èva S. Nagy, BMedSci(Hons), MBBS, PhD, FRACS, Grad Cert (Breast Surgery), Mark Westaway, MBBS, DCH, Grad Dip Allergy, Suzanne Danieletto, BSc, MBBS (Hons), FRCPA, MIAC, Lawrence B. Afrin, MD

“…Subjects and CG1 patients completed BII symptom questionnaires preoperatively and multiple points postoperatively. With CD117 staining, average and maximum mast cell counts (MCCs) in resected tissues were determined. …”
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Post-injury treatment with 7,8-dihydroxyflavone attenuates white matter pathology in aged mice following focal traumatic brain injury by Georgios Michalettos, Fredrik Clausen, Elham Rostami, Niklas Marklund

“…Histological assessment with Luxol Fast Blue (LFB)/Cresyl Violet stain showed that administration of 7,8-DHF resulted in preserved white matter tissue at 2 and 7 dpi with no difference in cortical tissue loss at all investigated time points. …”
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Mechanism of TGIF1 on glycolipid metabolism disorders in mice with type 2 diabetes by Yuchen Li, Li Tao, Shikai Wang, Fuyan Bai, Liping Zheng, Lijun Hou

“…The levels of fasting plasma glucose, fasting serum insulin, triglycerides, total cholesterol, low-density lipoprotein cholesterol, and high-density lipoprotein cholesterol were measured. Staining assays were performed to observe liver tissue pathology and lipid accumulation. …”
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Immune Dysregulation and Cellular Composition in Lichen Sclerosus Revealed by Integrative Epigenetic Analysis with Cell Type Deconvolution by Wang J, Fan H, Bao Z, Li G, Wang L, Zhang D

“…Immunohistochemical staining confirmed the changes in T cells. Enrichment analysis of DMPs indicated significant impacts on fibroblast-related processes and key immune pathways. …”
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Bacillus coagulans alleviates hepatic injury caused by Klebsiella pneumoniae in rabbits. by Xiaoguang Chen, Wenjuan Wei, Fan Yang, Jianing Wang, Qiongxia Lv, Yumei Liu, Ziqiang Zhang

“…Liver tissues were harvested and processed for histological assessment using H&E and VG stains to assess structural alterations. Biochemical assays were employed to quantify the enzymatic activities of T-SOD and GSH-Px, as well as the MDA content. …”
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Sleeve gastrectomy improves cardiac function and glucose-lipid metabolism disorder in obese rats induced by a high-fat and high-sugar diet by Xiaojuan Li, Luming Qiao, Wei Wang, Ying Shi, Lei Yuan, Zhiwei Zhai

“…Furthermore, enhanced pathological changes, including inflammatory cell infiltration and loss of cytoplasm striations, enhanced oil red O staining, increased TUNEL-positive cells, upregulated Bax and cleaved-caspase-3, and downregulated Bcl-2, were observed in obese rats, which were notably alleviated by SG. …”
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ALKBH3-mediated m1A demethylation promotes the malignant progression of acute myeloid leukemia by regulating ferroptosis through the upregulation of ATF4 expression by Xin Liu, Xinghua Pan

“…Studies have shown that ALKBH3 is upregulated in most tumors, but the role of ALKBH3 in AML remains unclear.Methods: In this study, we investigated the function of ALKBH3 in AML cells (KG-1) by immunofluorescence, ELISA, flow cytometry, HE staining, and Western blotting.Results: Our results revealed that ALKBH3 is upregulated in AML and that the downregulation of ALKBH3 inhibited KG-1 cell proliferation and promoted cell apoptosis; at the same time, ALKBH3 upregulated ATF4 expression through m1A demethylation, and the knockdown of ATF4 resulted in increased ferrous iron content; TFR1, ACSL4, and PTGS2 expression; and ROS and MDA levels, whereas SOD and GSH levels and the expression levels of ATF4, SLC7A11, GPX4, and FTH1 decreased in KG-1 cells, thereby promoting ferroptosis. …”
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The Value of Immune-Related Genes Signature in Osteosarcoma Based on Weighted Gene Co-expression Network Analysis by Xin Wang, Li Gan, Ju Ye, Mengjie Tang, Wei Liu

“…The results of immunohistochemical staining and qPCR showed that EVI2B was a tumor suppressor gene. …”
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Anaemia Associated with Trypanosomes Infections in Cattle of West Gojjam Zone, Northwest Ethiopia by Kumela Lelisa, Behablom Meharenet

“…A cross-sectional study was conducted from December 2017 to January 2018 in West Gojjam zone, Northwest Ethiopia, to determine the trypanosome infections rate and the possible correlation between parasitic infection and anaemia using the dark ground buffy coat technique, Giemsa-stained thin blood smear, and PCV reading on a haematocrit reader. …”
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Impact of PCSK9 Immunization on Glycemic Indices in Diabetic Rats by Amir Abbas Momtazi-Borojeni, Mahmoud Reza Jaafari, Elham Abdollahi, Maciej Banach, Amirhossein Sahebkar

“…Histopathology examination of the liver and pancreas was also performed using the hematoxylin-eosin staining method. Results. The prepared nanoliposomal vaccine could strongly induce anti-PCSK9 antibodies in the vaccinated rats. …”
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