Inhibition of NF-κB/IL-33/ST2 Axis Ameliorates Acute Bronchiolitis Induced by Respiratory Syncytial Virus by Liwen Zhang, Yu Wan, Liang Ma, Kaihong Xu, Baojin Cheng

“…Enzyme-linked immunosorbent assay (ELISA), quantitative real time PCR, western blot analysis, immunohistochemical staining, and flow cytometry analysis were performed to examine relevant indicators. …”
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Thrombin Induces COX-2 and PGE2 Expression via PAR1/PKCalpha/MAPK-Dependent NF-kappaB Activation in Human Tracheal Smooth Muscle Cells by Chien-Chung Yang, Li-Der Hsiao, Ya-Fang Shih, Chih-Kai Hsu, Chia-Yu Hu, Chuen-Mao Yang

“…The role of NF-κB was assessed by a chromatin immunoprecipitation (ChIP) assay, immunofluorescent staining, as well as Western blot. Our results verified that thrombin markedly triggered PGE2 secretion via COX-2 upregulation which were diminished by the inhibitor of thrombin (PPACK), PAR1 (SCH79797), Gi/o protein (GPA2), Gq protein (GPA2A), PKCα (Gö6976), p38 MAPK (SB202190), JNK1/2 (SP600125), MEK1/2 (U0126), or NF-κB (helenalin) and transfection with siRNA of PAR1, Gqα, Giα, PKCα, JNK2, p38, p42, or p65. …”
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Effects of orally exposed SiO2 nanoparticles on lipid profiles in gut-liver axis of mice by Kuanhang Li, Zhengzheng Zhou, Yi Cao

“…After 5 days exposure (once a day), we observed that SiO2 NPs induced minimal pathological changes but increased most of the trace elements. Furthermore, lipid staining was gradually decreased in intestines and livers with the increase of NP levels. …”
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Development and Evaluation of a Loop-Mediated Isothermal Amplification Assay for Diagnosis of Schistosoma mansoni Infection in Faecal Samples by Ibrahim N. Mwangi, Eric L. Agola, Robert M. Mugambi, Esther A. Shiraho, Gerald M. Mkoji

“…The amplified DNA was either visualized under ultraviolet light after electrophoresis or by directly observing the color change after staining the amplicons with CYBR Green dye. The LAMP assay was evaluated against the microscopy-based procedure and the results were analysed using Cohen’s kappa coefficient to determine the degree of agreement between the two techniques. …”
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CDC25B Is a Prognostic Biomarker Associated With Immune Infiltration and Drug Sensitivity in Hepatocellular Carcinoma by Zixiang Huang, Liangzhi Xu, Zhengqiang Wu, Xiaofeng Xiong, Linfei Luo, Zhili Wen

“…Gene set enrichment analysis (GSEA) was used to identify signaling pathways enriched in CDC25B expression, and differential genes (DEGs) were used to screen out coexpressed hub genes and construct protein-protein interaction (PPI) networks. 5-Ethynyl-2′-deoxyuridine (EDU) staining was used to compare the proliferation and differentiation ability of the HCC cell line (HCC-LM3) after knockdown of CDC25B. …”
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Human Defence Factors in Different Gestational Week Placenta: A Pilot Study by Andris Kamergrauzis, Mara Pilmane, Anna Junga

“…Methods: A total of 15 human placental tissue samples were acquired from mothers with different gestational weeks: 28, 31, and 40. Routine staining and immunohistochemistry for the samples were executed. …”
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ATP Induces Disruption of Tight Junction Proteins via IL-1 Beta-Dependent MMP-9 Activation of Human Blood-Brain Barrier In Vitro by Fuxing Yang, Kai Zhao, Xiufeng Zhang, Jun Zhang, Bainan Xu

“…ELISA, Western blot, and immunofluorescent staining procedures were used. Our data demonstrated that exposure to the photoreactive ATP analog, 3′-O-(4-benzoyl)benzoyl adenosine 5′-triphosphate (BzATP), induced a significant decrease in ZO-1 and occludin expression. …”
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TMEM147 Correlates with Immune Infiltration and Serve as a Potential Prognostic Biomarker in Hepatocellular Carcinoma by Sheng Cheng, Jutang Li, Ming Xu, Qun Bao, Jiaoxiang Wu, Peng Sun, Bo Han

“…In addition, we examined the associations between TMEM147 expression and immune infiltration using single-sample gene set enrichment analysis (ssGSEA) and immunofluorescence staining of HCC tissues. Results. Our results showed that the expression of TMEM147 was significantly higher in human HCC tissues than in adjacent normal liver tissues, with similar findings in human HCC cell lines. …”
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Perivascular Invasion: A Promising Prognostic Parameter for Breast Cancer by Begüm Çalım GÜRBÜZ, Tuçe Söylemez AKKURT, Handan EREN, Hazal İzol ÖZMEN, Ebru ŞEN, Burçin PEHLİVANOĞLU

“…The presence of PVI was evaluated on hematoxylin&eosin stained slides, and the association between PVI and clinicopathological parameters was statistically analyzed. …”
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Effects of Ghrelin on Triglyceride Accumulation and Glucose Uptake in Primary Cultured Rat Myoblasts under Palmitic Acid-Induced High Fat Conditions by Lingling Han, Jia Li, Ying Chen, Wei Wang, Dan Zhang, Guoliang Liu

“…Triglyceride accumulation was determined by Oil-Red-O staining and the glycerol phosphate dehydrogenase-peroxidase enzymatic method, and glucose uptake was determined by isotope tracer. …”
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Induction of labour outcomes in a tertiary hospital: the Ghanaian cervix and misoprostol 25ug (GCAM-25 STUDY)—a cross-sectional study by Teresa Aba Mensah, Kwaku Asah-Opoku, Kwame Adu-Bonsaffoh, Evans Kofi Agbeno

“…Fetal heart rate abnormalities occurred in 8.9% of participants, meconium staining of liquor occurred in twenty-five participants (14.9%). …”
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Cytotoxic Constituents from the Rhizomes of Curcuma zedoaria by Omer Abdalla Ahmed Hamdi, Syarifah Nur Syed Abdul Rahman, Khalijah Awang, Norhanom Abdul Wahab, Chung Yeng Looi, Noel Francis Thomas, Sri Nurestri Abd Malek

“…Curcumenone (3) and curcumenol (5) displayed strong antiproliferative activity (IC50=8.3±1.0 and 9.3±0.3 μg/mL, resp.) and were found to induce apoptotic cell death on MCF-7 cells using phase contrast and Hoechst 33342/PI double-staining assay. Thus, the present study provides basis for the ethnomedical application of Curcuma zedoaria in the treatment of breast cancer.…”
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Realization of malignant potential of human glioblastoma mitochondria in immunodeficient animals by O. I. Kit, E. M. Frantsiyants, A. I. Shikhlyarova, I. V. Neskubina

“…Morphological examination of the mesentery and lymph nodes revealed multiple areas with atypical cells resembling glioblastoma multiforme, localized in fibrous septa and sinuses. Stained granules with the corresponding immunohistochemical profile, GFAP and ki-6, were clearly defined in the mesentery and lymph node of a Balb/c Nude mouse (N = 6) aſter transplantation of human glioblastoma mitochondria. …”
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The impact of Sudafed on spleen of albino mice by L. Alsaadi Yasmin, K. Abdul Ameer Bassam, Jasim Mohamad Ban, Kathum Omra Faiza

“…After the mentioned period, the mice of all subgroups were sacrificed and the spleens were removed, processed, sectioned and stained for histological analysis. Results showed that the amount of PSE administered at 500 mg/kg caused considerable sago spleen, numerous amyloid depositions in peripheral zone, sinusoidal red pulp congestion, and hyperplasia inside the germinal center. …”
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Substrate curvature influences cytoskeletal rearrangement and modulates macrophage phenotype by Austin Sovar, Matthew D. Patrick, Ramkumar T. Annamalai

“…Macrophages were seeded onto these microgels, and cytoskeletal dynamics were examined using confocal microscopy, SEM, and actin-specific staining. Gene expression of pro- and anti-inflammatory markers was quantified using qPCR. …”
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Characterization of Helicobacter Pylori Infection in Patients with Gastric Ulcer by Marcos Félix Osorio Pagola, Magalys Blanca Olivert Cruz, Juan Luís de Pasos Carrazana, Alfredo Basilio Quiñones Ceballos, Mabel Vega Galindo, Anagalys Ortega Alvelay

“…Endoscopy and mucous gastric biopsy were performed for the histological and diagnostic study of the infection due to Helicobacter Pylori by means of the hematoxiline-eosine and giemsa stain respectively. The sample was composed by 137 patients. …”
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Effectiveness and safety of herbal medicines for induction of labour: a systematic review and meta-analysis by Edward Fottrell, Carina King, Chrispin Mandiwa, Collins Zamawe, Hannah Maria Jennings

“…No significant difference in the incidence of caesarean section (RR 1.19; 95% CI 0.76 to 1.86), assisted vaginal delivery (RR 0.73; 95% CI 0.47 to 1.14), haemorrhage (RR 0.84; 95% CI 0.44 to 1.60), meconium-stained liquor (RR 1.20; 95% CI 0.65 to 2.23) and admission to nursery (RR 1.08; 95% CI 0.49 to 2.38) was found between users and non-users of herbal medicines for IOL.Conclusions The findings suggest that herbal medicines for IOL are effective, but there is inconclusive evidence of safety due to lack of good quality data. …”
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SIRT1/PGC-1α-mediated mitophagy participates the improvement roles of BMAL1 in podocytes injury in diabetic nephropathy: evidences from in vitro experiments by Yanxia Rui, Yinfeng Guo, Linying He, Min-er Wang, Henglan Wu

“…Mitophagy was examined by detecting autophagosomes using transmission electron microscopy, and detecting the colocalization of LC3 and Tom20 using immunofluorescence staining. The interaction between BMAL1 and SIRT1 was conducted by immunoprecipitation (Co-IP) assay. …”
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Multifunctional properties of cotton fabric tailored via green synthesis of TiO2/curcumin composite by Barbara Simončič, Brigita Tomšič, Marija Gorjanc, Mateja Kert

“…All chemically modified CO samples showed photocatalytic activity, degrading coffee stains and decolourising methylene blue and Rhodamine B dye solutions. …”
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RETRACTED ARTICLE: Combined treatment of glibenclamide and CoCl2 decreases MMP9 expression and inhibits growth in highly metastatic breast cancer by Zhe Rong, Li Li, Fei Fei, Lailong Luo, Yang Qu

“…Some fresh tissues without necrosis were stored at −80°C for mRNA detection and the other tumor tissue was fixed with 10% formalin for H&E and immunohistochemical staining. Results The growth rate of tumor cells in the CoCl2 + glibenclamide group was lower than that seen in the other groups. …”
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