miR-223 promotes cartilage differentiation of bone marrow-derived mesenchymal stem cells and protects against osteoarthritis by suppressing NLRP-3 expression by Nan Min, Jie Ma, Lei Shi, Lin Wang, Chi Liu, Yaonan Zhang, Qingyun Xue

“…Material and methods Bone marrow-derived mesenchymal stem cells (BMSCs) were isolated from rats and cultured in chondrogenic medium to stimulate the differentiation of chondrocytes. Alcian blue staining was performed to determine the chondrogenic differentiation and expression of miR-223 in the BMSCs. …”
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Additively manufactured stochastic and gyroid scaffold design towards osseointegration and bone regeneration in a rabbit femur model by Susheem Kanwar, Oraib Al-Ketan, Gopinathan Janarthanan, Sanjairaj Vijayavenkataraman

“…While the performance differences between porous designs were minimal, the stochastic scaffolds demonstrated slightly superior scores and staining results compared to gyroid scaffolds.…”
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Gene Therapy with HSV1-sr39TK/GCV Exhibits a Stronger Therapeutic Efficacy Than HSV1-TK/GCV in Rat C6 Glioma Cells by Lei-qing Li, Fang Shen, Xiao-yan Xu, Hong Zhang, Xiao-feng Yang, Wei-guo Liu

“…Then the in vivo sensitivity of these transfected C6-TK and C6-sr39TK cells to GCV was determined by 3-(4,5)-dimethylthiahiazo-(-z-y1)-3,5-di-phenytetrazoliumromide (MTT) colorimetric assay and Hoechst-propidium iodide (PI) staining. Finally, a subcutaneously C6 xenograft tumor model was established in the nude mice to test the in vitro efficacy of TK/GCV gene therapy. …”
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The effect of cacao bean extracts on the prevention of periodontal tissue breakdown in diabetic rats with orthodontic tooth movements by Nuzulul Hikmah, Mohamad Hidayat, Nur Permatasari, Aulanni'am Aulanni'am, Achmad Rudijanto

“…NFκB, Collagen-1, and FGF-2 expression were then assessed using the immunohistochemical method, while the number of fibroblasts and blood vessels was observed using hematoxylin-eosin stained tissue. The data obtained were analyzed with one-way ANOVA and post hoc tests, with p < 0.05. …”
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Intracellular iron accumulation throughout the progression of sepsis influences the phenotype and function of activated macrophages in renal tissue damage by Mira Hanna, Ahmed M. A. Akabawy, Mohamed Mansour Khalifa, Mohamed Mansour Khalifa, Marawan Abd Elbaset, Reda Abdelnasser Imam, Hanan Seddiek

“…Our results indicated severe renal tissue damage with a progressive increase in serum BUN and creatinine with architectural tissue damage and positive PAS staining. There was increased expression of CD8+ CD68+ M1 macrophage markers with upregulation of iNOS and co-expression of CD163+. …”
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Direct Effect of Two Naphthalene-Sulfonyl-Indole Compounds on Toxoplasma gondii Tachyzoite by Qasem Asgari, Hossein Keshavarz, Mostafa Rezaeian, Mohammad Hossein Motazedian, Saeedeh Shojaee, Mehdi Mohebali, Ramin Miri

“…Tachyzoites of RH strain were incubated in various concentrations (25–800 μM) of 1-(naphthalene-2-sulfonyl)-2,3-dihydro-1H-indole and 1-[5-(2,3-dihydro-1H-indole-1-sulfonyl)naphthalene-1-sulfonyl]-2,3-dihydro-1H-indole for 1.5 hours. Then, they were stained by PI and analyzed by FACS. To evaluate the infectivity, 2×106 tachyzoites exposed to the concentrations mentioned above were intraperitoneally inoculated into five mice from each group. …”
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Sjögren’s Syndrome: Concerted Triggering of Sicca Conditions by Zygmunt Mackiewicz, Justyna Mażul, Ieva Narkevičiūtė, Irena Dumalakienė, Irena Butrimienė, Rita Vilienė, Indrė Stankevičienė, Diana Mieliauskaitė

“…Significant correlations between immunohistochemical staining and serological findings were revealed. Conclusions. …”
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CD34 evaluation of microvasculature in lung adenocarcinoma and its microvascular density predicts postoperative tumor recurrence by Zijian Qiu, Jiaji Wu, Guanchao Pang, Xia Xu, Jun Lin, Pingli Wang

“…Immunohistochemical (IHC) staining was carried out using a CD34 polyclonal antibody to determine intratumoral MVD, and the relationship of CD34-MVD with the clinicopathological characteristics and survival time of LUAD patients was analyzed.ResultsCD34-MVD was associated with tumor size, lymph node metastasis, tumor recurrence, and patient survival status; patients with tumor size ≤3 cm (P = 0.015), negative for lymph node metastasis (P = 0.049), no tumor recurrence (P = 0.021), and survival (P = 0.042) had higher MVD. …”
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Palm Tocotrienol Activates the Wnt3a/β-Catenin Signaling Pathway, Protecting MC3T3-E1 Osteoblasts from Cellular Damage Caused by Dexamethasone and Promoting Bone Formation by Norfarahin Abdullah Sani, Nur Aqilah Kamaruddin, Ima Nirwana Soelaiman, Kok-Lun Pang, Kok-Yong Chin, Elvy Suhana Mohd Ramli

“…Cell viability was measured by the MTS assay. Alizarin Red staining was performed to detect calcium deposits. …”
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Concerning the issue of early diagnostics of low tannin content in faba bean seeds (<i>Vicia faba</i> L.) by S. M. Mamedova, V. S. Popov, A. E. Solovyeva, I. N. Perchuk, L. L. Malyshev, M. A. Vishnyakova

“…The absence of anthocyanin staining on seedlings and the absence of dark-colored extrafloral nectaries on stipules can serve as markers of low-tannin genotypes in the early stages of plant development. …”
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The Histone Demethylase KDM3B Promotes Osteo-/Odontogenic Differentiation, Cell Proliferation, and Migration Potential of Stem Cells from the Apical Papilla by Chen Zhang, Xiao Han, Yuncun Liang, Huina Liu, Zhipeng Fan, Jianpeng Zhang

“…By an alkaline phosphatase (ALP) activity assay, Alizarin red staining, real-time RT-PCR, and western blot analysis, we found that KDM3B enhanced the ALP activity and mineralization of SCAPs and promoted the expression of runt-related transcription factor 2 (RUNX2), osterix (OSX), dentin sialophosphoprotein (DSPP), and osteocalcin (OCN). …”
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Inactivation of glutathione S-transferase alpha 4 blocks Enterococcus faecalis-induced bystander effect by promoting macrophage ferroptosis by Yuanyuan Ju, Chunhua Ma, Lin Huang, Yumei Tao, Tianqi Li, Haibo Li, Mark M. Huycke, Yonghong Yang, Xingmin Wang

“…We found that, unlike Il10−/− mice, Gsta4−/− and Il10−/−/Gsta4−/− mice colonized with E. faecalis failed to develop colitis or CRC. Immunofluorescent staining showed a reduction of macrophages in the lamina propria of E. faecalis-colonized Il10−/−/Gsta4−/− mice, as well as decreased Gpx4 expression, indicating the occurrence of ferroptosis. …”
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Involvement of RBP-J interacting and tubulin-associated protein in the distribution of protein regulator of cytokinesis 1 in mitotic spindles by Julia Caspers, Andreas Ritter, Badi Bahrami, Samira Catharina Hoock, Susanne Roth, Alexandra Friemel, Franz Oswald, Frank Louwen, Nina-Naomi Kreis, Juping Yuan

“…Reduction of RITA enlarges the staining area of PRC1 in mitotic spindles as well as in the central spindle. …”
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Exploratory Investigation of Intestinal Structure and Function after Stroke in Mice by Diya Ye, Yuting Hu, Ning Zhu, Weizhong Gu, Gao Long, Enfu Tao, Marong Fang, Mizu Jiang

“…Hematoxylin-eosin (HE) staining showed the accumulation of leucocytes in the intestinal mucosa. …”
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Identification of the AHP family reveals their critical response to cytokinin regulation during adventitious root formation in apple rootstock by Ke Li, Ke Li, Ke Li, Huan Li, Wei Ling Liang, Jing Ju Liu, Hui Yue Tian, Li Hu Wang, Li Hu Wang, Yan Hong Wei, Yan Hong Wei

“…The results showed that MdAHP3 might be a key member in AR formation. GUS staining indicated that the activity of the MdAHP3 promoter was also significantly enhanced by CTK treatment. …”
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Activation of Melanocortin Receptors MC1 and MC5 Attenuates Retinal Damage in Experimental Diabetic Retinopathy by S. Rossi, R. Maisto, C. Gesualdo, M. C. Trotta, F. Ferraraccio, M. K. Kaneva, S. J. Getting, E. Surace, F. Testa, F. Simonelli, P. Grieco, F. Merlino, M. Perretti, M. D’Amico, C. Di Filippo

“…Similar changes were quantified with respect to Ki-67 staining. Finally, application of MC3-MC4 agonist/antagonists resulted to be inactive with respect to all parameters under assessment.…”
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Cancer Stem Cells from Tumor Cell Lines Activate the DNA Damage Response Pathway after Ionizing Radiation More Efficiently Than Noncancer Stem Cells by Heriberto Abraham Valencia-González, Graciela Ruíz, Elizabeth Ortiz-Sánchez, Alejandro García-Carrancá

“…To verify that our sphere cultures were enriched in CSC, we evaluated the double staining of CD49f and ALDH activity for HeLa cells by flow cytometry. …”
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A soy glycinin derived octapeptide protects against MCD diet induced non-alcoholic fatty liver disease in mice by Peng Ma, Rongrong Huang, Yu Ou

“…The results of oil red O staining and total cholesterol (TC)/triglyceride (TG) contents showed that SGP8 could significantly reduce the lipid content of steatosis HepG2 cells. …”
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Effect of extracellular vesicle ZNF280B derived from lung cancer stem cells on lung cancer progression by Qixia Guo, Jiayan Lu, Hui Zhao, Ding Zhou, Hua Liu

“…The interaction between lung CSCs-EVs and lung cancer cells was observed through PKH67 staining. Subsequently, we analyzed the differentially expressed genes in lung CSCs using bioinformatics data analysis and evaluated the prognostic value of ZNF280B in lung cancer with the Kaplan-Meier Plotter. …”
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C3AR1 as a target for preeclampsia: from bioinformatics and network pharmacology to experimental validation by Yongfeng Hu, Shaoqiang Li, Yunhui Hong, Dongxian Peng

“…Immunohistochemical staining of 80 placental tissues (40 early-onset preeclampsia and 40 healthy controls) confirmed C3AR1 expression and its clinical correlation. …”
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