Recurrent Helicobacter cinaedi bacteremia cured with long-term oral minocycline treatment in a patient undergoing hemodialysis: a case report and literature review by Yutaka Kakizoe, Yasunobu Iwata, Terumasa Nakagawa, Yoshikazu Miyasato, Teruhiko Mizumoto, Yuichiro Izumi, Takashige Kuwabara, Masataka Adachi, Hideki Yokoi, Masashi Mukoyama

“…The clinical challenge with H. cinaedi infection includes its slow growth rate and weak Gram staining that make it easy to misdiagnose, as well as the high incidence of relapses after the discontinuation of antibiotics. …”
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Occurrence and Assessment of Human Exposure to Microplastic Particles in Bottled Water From Southwest, Nigeria by Oluwatosin Shokunbi, Ebunoluwa Akinbile, David Jegede, Oluwawapelumi Oyedele, Olutayo Shokunbi

“…Methods: This study was designed to (a) determine the quantity, form, color, and size of MPs, (b) evaluate the influence of physicochemical properties on the release of MPs in bottled water, and (c) estimate the daily intake (EDI) of MPs in eight distinct varieties of bottled water from Southwest Nigeria. The Rose Bengal staining method and microscopic analysis were employed to identify, quantify, and characterize the MPs. …”
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Annotation-free deep learning algorithm trained on hematoxylin & eosin images predicts epithelial-to-mesenchymal transition phenotype and endocrine response in estrogen receptor-po... by Kaimin Hu, Yinan Wu, Yajing Huang, Meiqi Zhou, Yanyan Wang, Xingru Huang

“…To confirm the presence of morphological discrepancies in tumor tissues of ER+ breast cancer classified as epithelial- and mesenchymal-phenotypes according to EMT-related transcriptional features, we trained deep learning algorithms based on EfficientNetV2 architecture to assign the phenotypic status for each patient utilizing hematoxylin & eosin (H&E)-stained slides from The Cancer Genome Atlas database. …”
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Expressions of MMPs and TIMP-1 in Gastric Ulcers May Differentiate H. pylori-Infected from NSAID-Related Ulcers by Hsiu-Chi Cheng, Hsiao-Bai Yang, Wei-Lun Chang, Wei-Ying Chen, Yi-Chun Yeh, Bor-Shyang Sheu

“…The 126 gastric ulcer patients (H. pylori infected n=46; NSAID related n=30; combined with two factors n=50) provided ulcer and nonulcer tissues for assessment of MMP-3, -7, and -9 and TIMP-1 expression by immunohistochemical staining. Results. Gastric ulcer tissues had significantly higher MMP-3, -7, and -9 and TIMP-1 expressions than nonulcer tissues (P<0.05). …”
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Regeneration of Rabbit Auricular Cartilage After the Intravenous Stem Cell Injection by Pavlo Virych, Nadiia Shuvalova, Anton Karas, Galina Karas, Svitlana Chaika, Tetiana Kucherenko, Ganna Minina, Marina Timchenko, Oleg Melnykov, Yurii Minin

“…Histological examinations of the tissues were carried out after staining with fuchsin-eosin, azure II–eosin, and according to Weigert. …”
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Clinicopathological characteristics and gene mutations in 11 patients with lipoprotein glomerulopathy by Yan Qin, Xiao-Jing Sun, Yi-Fang Hu, Meng Jing, Xiao-Juan Yu, Ming-Hui Zhao, Ying Tan

“…In all renal specimens, there was evident dilation of glomerular capillary lumens containing lipoprotein thrombi, and positive oil red O staining was observed in frozen sections of all samples. …”
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Mutated p53 Promotes the Symmetric Self-Renewal of Cisplatin-Resistant Lung Cancer Stem-Like Cells and Inhibits the Recruitment of Macrophages by Yu Xu, Zhi Xu, Qi Li, Liang Guo, Yao Wang, Jianchun Zhou, Guansong Wang, Yuliang Liu

“…CD44+/CD90+ stem-like cells were identified in above cisplatin-resistant lung cancers (termed as cisplatin-resistant lung cancer stem-like cells, (Cr-LCSCs)) and stained with PKH26 dye which was used to define the self-renewal pattern. …”
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Differentiation of Rat Adipose-Derived Stem Cells into Parathyroid-Like Cells by Ping Zhang, Hao Zhang, Wenwu Dong, Zhihong Wang, Yuan Qin, Changhao Wu, Qi Dong

“…Adipogenic differentiation and osteogenic differentiation of the cells were identified by oil red O and alizarin red S staining, respectively. The adipose-derived stem cells were stimulated by sonic hedgehog (SHH) and activin A. …”
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Acupoint catgut embedding alleviates experimental autoimmune encephalomyelitis by modulating neuroinflammation and potentially inhibiting glia activation through JNK and ERK pathwa... by Xiaofang Liu, Liansheng Yang, Zhumin Su, Xueying Ma, Yingying Liu, Lili Ma, Xiaomeng Ma, Mingxia Ma, Xiaoyun Liu, Kun Zhang, Kun Zhang, Xiaohong Chen

“…RNA sequencing was performed to uncover ACE-associated transcriptional signatures in the spinal cords of EAE mice.ResultsThe results were validated through western blotting, qRT-PCR, and immunofluorescence (IF) staining. In ACE-treated mice, EAE disease severity was significantly ameliorated, along with improvements in anxiety-like behaviors and reduced inflammation and demyelination. …”
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Lipid nephrotoxicity mediated by HIF-1α activation accelerates tubular injury in diabetic nephropathy by Zebo Hu, Qianwen Zhu, Ying Wang, Xue Deng, Hui Yang, Mingjun Zhou, Jiyuan Zhang, Hao Wang, Haosen Wang, Lin Wang, Cui Zhang, Shu Li

“…Meanwhile, lipid accumulation in the tubules of the DM + YC-1 group was markedly decreased in comparison to the DM + DMSO group. Accordingly, PAS staining revealed that the pathological injury caused to the tubular epithelial cells was alleviated by YC-1 treatment. …”
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Proapoptotic and Antiangiogenic Activities of Arctium Lappa L. on Breast Cancer Cell Lines by Mohamad Taleb Agha, Hussein M. Baharetha, Majed Ahmed Al-Mansoub, Yasser M. Tabana, Nur Hidayah Kaz Abdul Aziz, Mun Fei Yam, Amin Malik Shah Abdul Majid

“…Morphological alterations were visualized using Hoechst staining and a transmission electron microscope. Cancer cell signal transduction pathways were investigated, and EHX significantly upregulated p53, TGF-β, and NF-κB. …”
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Identification of nuclear valosin-containing-protein-like as a target of anti-nuclear autoantibodies in systemic sclerosis by Zitao Zeng, Ramona Miske, Madeleine Scharf, Yvonne Denno, Anthonina Ott, Stefanie Brakopp, Bianca Teegen, Winfried Stöcker, Elise Siegert, Elise Siegert, Sandra Saschenbrecker, Christian Probst, Lars Komorowski

“…The prevalence of autoantibodies against this antigen was studied in 693 patients with systemic autoimmune rheumatic diseases (SARD) and 150 healthy controls.ResultsThe index patient serum displayed a homogeneous nucleolar staining pattern on HEp-2 cells and monkey liver by IFA but did not react with 27 known nuclear antigens. …”
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Impact of DLL3 Expression as Prognostic Factor in Extensive Stage of Small Cell Lung Cancer Treated With First‐Line Chemotherapy by Hohyung Nam, Soon‐Hee Jung, Jii Bum Lee, Jee Hyun Kong, Seungtaek Lim

“…In addition, tissue specimen should be available for immuno‐histochemical staining for DLL3, and their clinico‐pathologic data, including progression‐free survival (PFS) and overall survival (OS), were obtained. …”
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Cryopreservation of epididymal semen of domestic cat by S. Ya. Amstislavsky, E. Yu. Brusentsev, V. I. Mokrousova, E. A. Kizilova, V. V. Kozhevnikova, V. A. Naprimerov, I. N. Rozhkova

“…The viability of frozen-thawed spermatozoa was evaluated by VitalScreen kit, staining with hematoxylin and eosin was performed for analysis of the spermatozoa morphology; both analyses were combined with the light microscopy. …”
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Bushen Huoxue formula protects against renal fibrosis and pyroptosis in chronic kidney disease by inhibiting ROS/NLRP3-mediated inflammasome activation by Lin Liao, Pengyu Tao, Qiming Xu, Jie Chen, Weiwei Liu, Jing Hu, Jianrao Lu

“…However, its mechanisms of action remain unclear.Methods and results In this study, a rat model of renal fibrosis was constructed by 5/6 nephrectomy in vivo, and histopathological changes were analyzed using hematoxylin-eosin and Masson’s trichrome staining. Angiotensin II (Ang II) was used to establish an in vitro renal fibrosis cell model in vitro. …”
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Effects of Cholecalciferol on Key Components of Vitamin D-Endo/Para/Autocrine System in Experimental Type 1 Diabetes by Anna Mazanova, Ihor Shymanskyi, Olha Lisakovska, Lala Hajiyeva, Yulia Komisarenko, Mykola Veliky

“…CYP27A1, CYP2R1, CYP27B1, and VDR were assayed by RT-qPCR and Western blotting or visualized by immunofluorescence staining. Results. We demonstrated that T1D led to a decrease in blood 25OHD, which is probably due to the established downregulation of CYP27A1 and CYP2R1 expression. …”
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MOMAST^® Downregulates AQP3 Expression and Function in Human Colon Cells by Ines Angelini, Mariangela Centrone, Giusy Rita Caponio, Annarita Di Mise, Andrea Gerbino, Marianna Ranieri, Giovanna Valenti, Grazia Tamma

“…Confocal studies and Western Blotting analysis demonstrated that treatment with MOMAST^® significantly decreased the staining and the expression of AQP3. Importantly, functional studies revealed that the reduction of AQP3 abundance correlates with a significant decrease in glycerol and H₂O₂ uptake. …”
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Crispant analysis in zebrafish as a tool for rapid functional screening of disease-causing genes for bone fragility by Sophie Debaenst, Tamara Jarayseh, Hanna De Saffel, Jan Willem Bek, Matthieu Boone, Ivan Josipovic, Pierre Kibleur, Ronald Y Kwon, Paul J Coucke, Andy Willaert

“…Skeletal phenotyping at 7, 14, and 90 days post-fertilization (dpf) using microscopy, Alizarin Red S staining, and microCT was performed. Larval crispants showed variable osteoblast and mineralization phenotypes, while adult crispants displayed consistent skeletal defects, including malformed neural and haemal arches, vertebral fractures and fusions, and altered bone volume and density. …”
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Infiltration and subtype analysis of CD3 + CD20 + T cells in lung cancer by Liping Luo, Juan Ji, Juan Dong, Maotao He, Wenjun Jiang, Yang Liu, Weidong Wang

“…Methods Lung cancer surgical samples were stained using multi-color immunofluorescence to study the subtypes and distribution patterns of TB cells. …”
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Development of therapeutic supplement using roasted-cashew-nut to protect cerebral vasoconstriction injury triggered by mixture of petroleum hydrocarbons in the hypothalamus and hi... by J.K. Akintunde, V.O. Akomolafe, R.N. Ugbaja, A.M. Olude, A.D. Folayan

“…Cell pathological evaluation was done using hematoxylin and eosin staining and visualized under the microscope. Biochemical and molecular markers of brain vasoconstriction injury (BVI) were evaluated using spectrophotometer and RT-PCR analyzer, respectively. …”
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