Protective Effects of Necrostatin-1 against Concanavalin A-Induced Acute Hepatic Injury in Mice by Yingqun Zhou, Weiqi Dai, Chunlei Lin, Fan Wang, Lei He, Miao Shen, Ping Chen, Chenfen Wang, Jie Lu, Ling Xu, Xuanfu Xu, Chuanyong Guo

“…The expression of TNF-α, RIP1, and LC3 was detected with immunohistochemical staining. The expression of TNF-α, IFN-γ, IL2, IL6, caspase 3, RIP1, beclin-1, and LC3 protein was assessed by immunofluorescence and western blotting. …”
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Impact of Long-term Polyvinylpyrrolidone Exposure on Gene Expression, Chromatin Structure, and Malondialdehyde Levels in Human Sperm by Mojdeh Sabour, Mahmood Dehghani Ashkezari, Azam Agharahimi, Seyed Morteza Seifati, Seyed Mehdi Kalantar

“…This study was designed to evaluate the effects of this component on chromatin quality with acridine orange (AO) staining, Malondialdehyde (MDA) levels, and BAX, BCL2, HSP70 (HSPA2), and SOD2 genes expression in different time intervals (0, 15, 30, and 60 minutes). …”
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Modulation of Caecal Microbiome in Obese Mice Associated with Administration of Amaranth or Soybean Protein Isolates by Diana Olguín-Calderón, Jorge L. González-Escobar, Rosalva Ríos-Villa, Elena Dibildox-Alvarado, Antonio De León-Rodríguez, Ana P. Barba de la Rosa

“…Morphological changes in caecum ultrathin sections were measured after hematoxylin/eosin staining. Microbiota was isolated from the caecum and 16S rRNA gene was sequenced. …”
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Activation of Opioid Receptors Attenuates Ischemia/Reperfusion Injury in Skeletal Muscle Induced by Tourniquet Placement by Yue-Xian Guo, Gui-Ying Wang, Wen-jie Cheng, Cai-Zhen Yan, Shuang Zhao, Zhao Li, Peng Liu, Xiu-Li Wang

“…Morphological changes of the gastrocnemius muscle in these mice were assessed by hematoxylin-eosin (HE) staining and electron microscopy. Expression levels of TLR4, NF-κB, SIRT1, and PGC-1α in the skeletal muscles were detected by western blot. …”
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TrkB Receptor Antagonism Enhances Insulin Secretion and Increases Pancreatic Islet Size in Rats Fed a Cafeteria-Style Diet by Jorge Agustín Velasco-Gutierrez, Elena Roces de Alvarez-Buylla, Sergio Montero, Alejandrina Rodríguez-Hernández, Saraí Limón Miranda, Karmina Martínez-Santillan, María del Rosario Álvarez-Valadez, Mónica Lemus, Alejandra Flores-Silva, Adolfo Virgen-Ortiz

“…After 4 months of intervention, the glucose and insulin tolerance curves, serum insulin levels, body fat and hematoxylin-eosin staining pancreas were evaluated. <b>Results:</b> The results showed that the cafeteria-style diet induced an increase in the amount of body fat, alterations in the glucose tolerance curve, increased insulin circulation levels, increased HOMA indices and increased pancreatic islet size. …”
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Pleura-Based Lipomatous Neoplasm with RUNX1T1::PLAG1 Rearrangement and RB1 Gene Deletion by Rayan Sibira, Anna Vu, Ryan Martinez, Emilian Racila, Siddhartha Sen, Diana Oramas

“…Immunohistochemistry revealed diffuse expression of CD34 in spindle cells, mosaic staining of RB1, diffuse positivity for p16, and patchy positivity for desmin, while adipocytes were positive for S100. …”
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Immunophenotypic Characterization of Human Bone Marrow Mast Cells. A Flow Cytometric Study of Normal and Pathological Bone Marrow Samples by Luis Escribano, Alberto Orfao, Jesús Villarrubia, Beatriz Díaz-Agustín, Carlos Cerveró, Agustín Rios, José L. Velasco, Juana Ciudad, José L. Navarro, Jesús F. San Miguel

“…The goal of the present paper was to define the immunophenotype of bone marrow mast cells (BMMC) from healthy controls and patients with hematologic malignancies (HM) based on the use of multiple stainings with monoclonal antibodies analyzed by flow cytometry. …”
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Mechanical Strain-Mediated Tenogenic Differentiation of Mesenchymal Stromal Cells Is Regulated through Epithelial Sodium Channels by Hui Yin Nam, Malliga Raman Murali, Raja Elina Ahmad, Belinda Pingguan-Murphy, Hanumantha Rao Balaji Raghavendran, Tunku Kamarul

“…Analyses at these time points included cell morphology and alignment observation, immunocytochemistry and immunofluorescence staining (collagen I, collagen III, fibronectin, and N-cadherin), and gene expression (ENaC subunits, and tenogenic markers). …”
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Dual inhibition of BET and EP300 has antitumor activity in undifferentiated pleomorphic sarcomas and synergizes with ferroptosis induction by Stéphanie Verbeke, Aurélien Bourdon, Mathilde Lafon, Vanessa Chaire, Bertolo Frederic, Amina Naït Eldjoudi, Marie-Alix Derieppe, Francis Giles, Antoine Italiano

“…The most potent inhibitor was evaluated in vivo in two animal models and the mechanisms of action were investigated by RNA sequencing, Western blotting and immunofluorescence staining. A CRISPR knockout screen was performed to identify resistance mechanisms. …”
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Human parietal epithelial cells as Trojan horses in albumin overload by Giovanna Priante, Monica Ceol, Lisa Gianesello, Claudia Maria Radu, Rachele Mantese, Lucia Federica Stefanelli, Martina Cacciapuoti, Francesca K. Martino, Lorenzo Arcangelo Calò, Franca Anglani, Federico Nalesso, Dorella Del Prete

“…Cell morphology was analyzed by phase-contrast microscopy and F-actin staining. We demonstrate that hPECs internalize albumin via receptor-mediated mechanisms. …”
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Interleukin 10 Plays an Important Role in Neonatal Rats with Hypoxic-Ischemia Associated with B-Cell Lymphoma 2 and Endoplasmic Reticulum Protein 29 by Xue Bai, Liu-Lin Xiong, Chang-Le Fang, Hao-Li Zhou, Lu-Lu Xue, Yue Hu, Qing-Jie Xia, Jia Liu, Jun-Yan Zhang, Ting-Hua Wang, Si-Jin Yang

“…Immunofluorescence double staining was performed to observe the localization of IL-10 in neurons and astrocytes. …”
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Epigallocatechin-3-gallate inhibits the collagen accumulation of oral submucous fibrosis induced by arecoline by Ge Gao, Ge Gao, Caipeng Lin, Caipeng Lin, Ruibo Li, Xi Xie, Hai-Bin Luo, Hai-Bin Luo

“…The study also established an OSF model in Sprague-Dawley rats, induced by ARE, with pathological changes characterized using HE and Masson’s staining, further assessing the impact of ARE on ECM-related protein expression in rat oral tissues through RT-qPCR and Western blot methods.ResultsEGCG effectively suppressed the ARE-induced ECM components while concurrently improving the OSF pathological process in vitro and in vivo.ConclusionThe results indicate that the natural product EGCG effectively suppressed the increased ECM components induced by ARE and concurrently improved the OSF pathological process, indicating that EGCG could be potentially a novel anti-fibrotic candidate drug for the treatment of OSF.…”
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In Vivo Evaluation of the Combined Anticancer Effects of Cisplatin and SAHA in Nonsmall Cell Lung Carcinoma Using [18F]FAHA and [18F]FDG PET/CT Imaging by Skye Hsin-Hsien Yeh, Ming Hsien Lin, I. I. Leo Garcia Flores, Uday Mukhopadhyay, Danial Young, Kazuma Ogawa, Jeong-Hwan Jeong, William Tong, Juri G. Gelovani, Nobuyoshi Fukumitsu

“…Cisplatin alone significantly increased [18F] FAHA accumulation and reduced [18F] FDG accumulation in H441 and PC14 xenografts; coadministration of cisplatin and SAHA resulted in the opposite effects. Immunochemical staining for acetyl-histone H3 confirmed the PET/CT imaging findings. …”
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AFP shields hepatocellular carcinoma from macrophage phagocytosis by regulating HuR-mediated CD47 translocation in cellular membrane by Yinglian Pan, Qiushi Yin, Zhaoliang Wang, Gang Wu, Kun Liu, Xiaowei Li, Jinchen Liu, Jiangzheng Zeng, Bo Lin, Wei Li, Mingyue Zhu, Mengsen Li

“…Methods: Immunohistochemical and immunofluorescence staining was used to analyze the relativity of AFP and cellular membrane CD47 expression in clinical 30 HCC tissues, and the expression of AFP and CD47 in HCC cells. …”
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Placenta-derived mesenchymal stem cells protect against diabetic kidney disease by upregulating autophagy-mediated SIRT1/FOXO1 pathway by Honghong Liu, Jiao Wang, Guanru Yue, Jixiong Xu

“…The urine microalbumin/creatinine ratio was determined using ELISA, and renal pathological changes were detected by special staining techniques. Immunofluorescence was used for detecting the renal tissue expression of podocin and nephrin; immunohistochemistry for the renal expression of autophagy-related proteins (LC3, Beclin-1, SIRT1, and FOXO1); and western blotting and PCR for the expression of podocyte autophagy- and pathway-related indicators. …”
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Fish Tank Granuloma Presenting as a Nasal Cavity Mass by Motoki Sekine, Fumiyuki Goto, Kosuke Saito, Shoji Kaneda, Hikaru Yamamoto, Tomoaki Murakami, Takahide Hamano, Kenji Okami

“…The pathological finding was an inflammatory granuloma with negative Ziehl–Neelsen staining. The granuloma recurred 3 months after resection. …”
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Regulation of IL-33 by Oncostatin M in Mouse Lung Epithelial Cells by Carl D. Richards, Laura Izakelian, Anisha Dubey, Grace Zhang, Steven Wong, Karen Kwofie, Aatif Qureshi, Fernando Botelho

“…Immunohistochemistry for IL-33 indicated a marked induction of nuclear staining in alveolar epithelial cells in vivo. Oncostatin M stimulated IL-33 mRNA and IL-33 full length protein in C10 mouse type 2 alveolar epithelial cells in culture in time-dependent and dose-dependent fashion, whereas IL-6, LIF, IL-31, IL-4, or IL-13 did not, and TGFβ repressed IL-33. …”
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Single-cell and spatial characterization of plasmablast-like lymphoma cells in primary central nervous system lymphoma by Hiroki Kobayashi, Ryota Chijimatsu, Yusuke Naoi, Yoshihiro Otani, Ryo Mizuta, Kentaro Fujii, Joji Ishida, Hiroyuki Murakami, Hideki Ujiie, Kazuhiro Ikeuchi, Tomohiro Urata, Keisuke Seike, Hideaki Fujiwara, Noboru Asada, Nobuharu Fujii, Ken-ichi Matsuoka, Yasuharu Sato, Yoshinobu Maeda, Daisuke Ennishi

“…An expansion study showed that ∼40% of patients with PCNSL had a PBL signature subpopulation, as defined by CD138 immunohistochemistry staining. Additionally, patients with a PBL signature subpopulation and low CD3+ cell infiltration exhibited a worse prognosis. …”
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CZYH Alleviates β-Amyloid-Induced Cognitive Impairment and Inflammation Response via Modulation of JNK and NF-κB Pathway in Rats by Yuanyuan Deng, Lianzhi Ye, Cheng Yu, Caixia Yin, Jingshan Shi, Qihai Gong

“…The Morris water maze test revealed that CZYH (400 or 800 mg/kg) administration improved the Aβ25-35-induced cognitive impairments in rats, and Nissl staining demonstrated that CZYH mitigated the Aβ-caused neuron loss. …”
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Application of IgG-Derived Natural Treg Epitopes (IgG Tregitopes) to Antigen-Specific Tolerance Induction in a Murine Model of Type 1 Diabetes by Leslie P. Cousens, Yan Su, Elizabeth McClaine, Xin Li, Frances Terry, Robert Smith, Jinhee Lee, William Martin, David W. Scott, Anne S. De Groot

“…Upregulation of FoxP3 in KJ1-26-stained OVA-specific CD4+ T cells was observed following treatment of DO11.10 mice with Tregitopes, along with reductions in anti-OVA Ig and T effector responses. …”
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