Enhancing glioblastoma therapy: unveiling synergistic anticancer effects of Onalespib - radiotherapy combination therapy by Julia Uffenorde, Mehran Hariri, Eleftherios Papalanis, Annika Staffas, Josefine Berg, Bo Stenerlöw, Hanna Berglund, Christer Malmberg, Diana Spiegelberg, Diana Spiegelberg

“…In this study, we investigated the effect of HSP90 inhibition in combination with radiotherapy in established and patient-derived glioblastoma cell lines.MethodsPotential radiosensitizing effects of the HSP90 inhibitor Onalespib were studied in XTT and clonogenic survival assays as well as in tumor-mimicking multicellular spheroid models. Further, migration capacity and effects on protein expression were studied after exposure to Onalespib and radiation using Proximity Extension Assay analysis.ResultsHSP90 inhibition with Onalespib synergistically enhanced the radiosensitivity of glioblastoma cells grown in 2D and 3D models, resulting in increased cell death, reduced migration capacity and activation of the apoptotic signaling pathway. …”
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Treatment of a mutant KRAS lung cancer cell line with polyisoprenylated cysteinyl amide inhibitors activates the MAPK pathway, inhibits cell migration and induces apoptosis. by Matthew D Gregory, Kweku Ofosu-Asante, Jassy Mary S Lazarte, Pablo E Puente, Nada Tawfeeq, Nadine Belony, Yong Huang, Ite A Offringa, Nazarius S Lamango

“…The depletion of these key cytoskeletal proteins may account for the observed inhibition of cell migration and invasion, and spheroid invasion observed on exposure to NSL-YHJ-2-27 and NSL-YHJ-2-46. …”
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Magnolol induces cell death through PI3K/Akt‐mediated epigenetic modifications boosting treatment of BRAF‐ and NRAS‐mutant melanoma by Abdullah Al Emran, Brinda Reddy Chinna Chowdary, Farzana Ahmed, Heinz Hammerlindl, Antje Huefner, Nikolas K. Haass, Wolfgang Schuehly, Helmut Schaider

“…This was confirmed in a 3D spheroid model. The apoptosis‐inducing effect of magnolol was completely rescued by activating Akt suggesting a mechanism relying primarily on Akt signaling. …”
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Testicular biometrics of Murrah buffaloes in different age groups by Erly L. Carrascal-Triana, Jorge Mejía Lúquez, Divier Agudelo Gómez, Edison Julián Ramírez Toro

“…The TV was determined using the equation of the spheroid prolate and represented the average of both testes. …”
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Wilms tumor primary cultures capture phenotypic heterogeneity and facilitate preclinical screening by Lisa Götz, Jenny Wegert, Alireza Paikari, Silke Appenzeller, Sabrina Bausenwein, Christian Vokuhl, Taryn D. Treger, Jarno Drost, Christin Linderkamp, Dominik T. Schneider, Karen Ernestus, Steven W. Warman, Jörg Fuchs, Nils Welter, Norbert Graf, Sam Behjati, Rhoikos Furtwängler, Manfred Gessler

“…Adherent, spheroid and organoid cultures are also clearly distinguishable by their transcriptome. …”
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Novel banana lectin CAR-T cells to target pancreatic tumors and tumor-associated stroma by Malcolm K Brenner, Challice Bonifant, Norihiro Watanabe, Mary K McKenna, Daniel Brenner, Ada Ozcan, Maureen Legendre, Dafydd G Thomas, Christopher Ashwood, Richard D Cummings, David M Markovitz

“…In three-dimensional (3D) spheroid models, we measured H84T CAR T-cell disruption of PSC architecture, and T-cell infiltration by live imaging. …”
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Generation of Insulin-Producing Cells from Canine Adipose Tissue-Derived Mesenchymal Stem Cells by Takahiro Teshima, Keiji Okamoto, Kazuho Dairaku, Tomokazu Nagashima, Masaki Michishita, Ryohei Suzuki, Hirotaka Matsumoto, Hidekazu Koyama

“…Culturing AT-MSCs with the differentiation protocol under a two-dimensional culture system did not produce IPCs. However, spheroid-like small clusters consisting of canine AT-MSCs and human recombinant peptide μ-pieces developed under a three-dimensional (3D) culture system were successfully differentiated into IPCs. …”
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Co-isolation of human donor eye cells and development of oncogene-mutated melanocytes to study uveal melanoma by Yunxi Chen, Eva Jin, Mohamed Abdouh, Éric Bonneil, Daniel Alexander Jimenez Cruz, Thupten Tsering, Qianqian Zhou, Aurélie Fuentes-Rodriguez, Alexandra Bartolomucci, Alicia Goyeneche, Solange Landreville, Miguel N. Burnier, Julia V. Burnier

“…Particularly, Matrigel™ coating induced spheroid growth under certain coating thickness and cell seeding density but did not improve NCM metabolic activity. …”
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Preclinical Efficacy and Proteomic Prediction of Molecular Targets for s-cal14.1b and s-cal14.2b Conotoxins with Antitumor Capacity in Xenografts of Malignant Pleural Mesothelioma by Angélica Luna-Nophal, Fernando Díaz-Castillo, Vanessa Izquierdo-Sánchez, Jesús B. Velázquez-Fernández, Mario Orozco-Morales, Luis Lara-Mejía, Johana Bernáldez-Sarabia, Noemí Sánchez-Campos, Oscar Arrieta, José Díaz-Chávez, Jorge-Ismael Castañeda-Sánchez, Alexei-Fedorovish Licea-Navarro, Saé Muñiz-Hernández

“…However, only s-cal14.1b modified spheroid growth. Approximately 600 proteins exhibited important differential expression, which was more heterogeneous in H2452 vs MSTO-211H spheroids. …”
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Duodenal Organoids From Metabolic Dysfunction-Associated Steatohepatitis Patients Exhibit Absorptive and Barrier Alterations by Alia Hadefi, Morgane Leprovots, Gilles Dinsart, Maryam Marefati, Marjorie Vermeersch, Daniel Monteyne, David Pérez-Morga, Anne Lefort, Frédérick Libert, Laurine Verset, Claire Liefferinckx, Christophe Moreno, Jacques Devière, Eric Trépo, Marie-Isabelle Garcia

“…Despite global heterogeneity in growth patterns, MDEOs frequently exhibited cystic spheroid morphology. MDEOs displayed altered digestive potential associated with reduced mature absorptive cell fate, but they retained their lipid metabolic capacity, possibly mediated by lipid oxidation in stem/progenitor cells. …”
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Dimorphos’s Material Properties and Estimates of Crater Size from the DART Impact by Angela M. Stickle, Kathryn M. Kumamoto, Dawn M. Graninger, Mallory E. DeCoster, Wendy K. Caldwell, Jason M. Pearl, J. Michael Owen, Olivier Barnouin, Gareth S. Collins, R. Terik Daly, Isabel Herreros, Jens Ormö, Jessica Sunshine, Carolyn M. Ernst, Toshi Hirabayashi, Simone Marchi, Laura Parro, Harrison Agrusa, Megan Bruck Syal, Nancy L. Chabot, Andy F. Cheng, Thomas M. Davison, Elisabetta Dotto, Eugene G. Fahenstock, Fabio Ferrari, Martin Jutzi, Alice Lucchetti, Robert Luther, Nilanjan Mitra, Maurizio Pajola, Sabina Raducan, KT Ramesh, Andrew S. Rivkin, Alessandro Rossi, Paul Sánchez, Stephen R. Schwartz, Stefania Soldini, Jordan K. Steckloff, Filippo Tusberti, Kai Wünnemann, Yun Zhang

“…Images from DART’s DRACO camera revealed Dimorphos to be an oblate spheroid covered in boulders of varying sizes and shapes. …”
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CAR-CIK vs. CAR-T: benchmarking novel cytokine-induced killer cells as solid tumor immunotherapy in ErbB2+ rhabdomyosarcoma by Laura M. Moser, Laura M. Moser, Laura M. Moser, Laura M. Moser, Laura M. Moser, Catrin Heim, Sebastian E. Koschade, Sebastian E. Koschade, Sebastian E. Koschade, Sebastian E. Koschade, Sebastian E. Koschade, Philipp Wendel, Philipp Wendel, Philipp Wendel, Philipp Wendel, Süleyman Bozkurt, Sabine Harenkamp, Hermann Kreyenberg, Hermann Kreyenberg, Michael Merker, Michael Merker, Christian Münch, Christian Münch, Christian Münch, Elise Gradhand, Meike Vogler, Meike Vogler, Meike Vogler, Evelyn Ullrich, Evelyn Ullrich, Evelyn Ullrich, Evelyn Ullrich, Evelyn Ullrich, Halvard Bönig, Halvard Bönig, Jan-Henning Klusmann, Jan-Henning Klusmann, Jan-Henning Klusmann, Jan-Henning Klusmann, Peter Bader, Peter Bader, Peter Bader, Peter Bader, Peter Bader, Winfried S. Wels, Winfried S. Wels, Winfried S. Wels, Eva Rettinger, Eva Rettinger, Eva Rettinger, Eva Rettinger, Eva Rettinger

“…Long-term co-incubation with tumor spheroids led to expansion of CAR-CIK cells and enrichment of CD3+CD56+ TNK cells. …”
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Graphite in the slag during modification of cast iron by addition alloy by V. I. Stetsenko, A. G. Solovej

“…The results and the analysis of the graphite content in the slag during modification of cast iron by spheroidizing addition alloy are given.…”
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Nanoparticle accumulation and penetration in 3D tumor models: the effect of size, shape, and surface charge by Pierre Cybulski, Maria Bravo, Maria Bravo, Jim Jui-Kai Chen, Indra Van Zundert, Sandra Krzyzowska, Farsai Taemaitree, Hiroshi Uji-i, Hiroshi Uji-i, Hiroshi Uji-i, Johan Hofkens, Johan Hofkens, Susana Rocha, Beatrice Fortuni

“…In this study, we addressed the challenge of inconsistent NP behavior by systematically evaluating NP uptake in both 2D and 3D tumor models, and NP penetration in spheroids. Our results showed that larger NPs exhibited higher internalization rates in 2D models but limited penetration in 3D spheroids. …”
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EFFECTS OF AUSTENITIZATION ON STRUCTURE FORMATION СHROMO-MOLYBDENUM-VANADIUM STEEL AFTER HIGH TEMPERING by V. A. Lutsenko, T. N. Golubenko, O. V. Lutsenko, N. A. Glazunova

“…It is shown that the decline of the austenitization temperature promotes to reduce the micro-hardness values due to the intensification of spheroidizing of pearlite after the overcooling and high tempering. …”
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Establishment of a Model of Microencapsulated SGC7901 Human Gastric Carcinoma Cells Cocultured with Tumor-Associated Macrophages by Jin-Ming Zhu, Xiu-Lian Quan, Shi-Chao Han, Xue-Jun Fan, He-Ming Li, Shan-Shan Liang, Xi Chen, Ruo-Yu Wang, Xue-Ning Ji

“…The expression of PCNA was observed outside spheroids; however, VEGF was found in the entire spheroids. …”
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Three-dimensional dynamics of mesothelin-targeted CAR.CIK lymphocytes against ovarian cancer peritoneal carcinomatosis by Federica Galvagno, Valeria Leuci, Annamaria Massa, Chiara Donini, Ramona Rotolo, Sonia Capellero, Alessia Proment, Letizia Vitali, Andrea Maria Lombardi, Valentina Tuninetti, Lorenzo D’Ambrosio, Alessandra Merlini, Elisa Vigna, Giorgio Valabrega, Luca Primo, Alberto Puliafito, Dario Sangiolo

“…In a “floating-like” 3D context with floating OC spheroids, both tumor localization and killing by MSLN-CAR.CIK were significantly boosted by fluid flow. …”
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Phosphatidylserine on sperm head interact with Annexin A5 on oviduct luminal cilia to form a sperm reservoir in pigs by Lorraine Schmaltz, Elie Barakat, Renaud Fleurot, Rustem Uzbekov, Karine Reynaud, Ludivine Laffont, Guillaume Tsikis, Isabelle Mérour, Pascal Mermillod, Marie Saint-Dizier

“…Porcine ANXA5 was immunodetected with a strong signal on luminal cilia in the isthmus and in derived isthmic epithelial spheroids (IES). Exogenous PS between 0.01 and 0.1 µg/mL and recombinant porcine ANXA5 (rpANXA5) above 0.1 µg/mL inhibited sperm binding to IES without reducing sperm motility. …”
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APPLICATION OF MODIFYING ALLOYING ALLOY CONTAINING NANOSIZED POWDERS OF ACTIVE ELEMENTS IN PRODUCTION OF HIGH-STRENGTH CAST IRON WITH GLOBULAR GRAPHITE by A. S. Kalinichenko, A. G. Slutsky, V. A. Sheinert

“…Scientific and practical interest is the application of alloying alloy-modifiers for secondary treatment of high-strength cast iron to stabilize the process of spheroidization graphite and achieving higher physical-mechanical properties of castings. …”
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The Role of Molybdenum in the Formation of the Microstructure and Properties of Al-Cu Alloys by S. Stąpór, M. Górny, Ł. Gondek, B. Gracz, M. Kawalec

“…Most of the iron-manganese phases occurred in a more spheroidal form. Additionally, tests were carried out on higher molybdenum content in the alloy. …”
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