Fusion protein-based COVID-19 vaccines exemplified by a chimeric vaccine based on a single fusion protein (W-PreS-O) by Pia Gattinger, Luibov I. Kozlovskaya, Alexander S. Lunin, Olga S. Gancharova, Dina I. Sirazova, Dina I. Sirazova, Vasiliy D. Apolokhov, Egor S. Chekina, Ilya V. Gordeychuk, Ilya V. Gordeychuk, Alexander V. Karaulov, Alexander V. Karaulov, Rudolf Valenta, Rudolf Valenta, Rudolf Valenta, Rudolf Valenta, Aydar A. Ishmukhametov, Aydar A. Ishmukhametov

“…The protein-based vaccine approach is exemplified solely in a proof of concept study by using W-PreS-O, a chimeric vaccine based on a single fusion protein (W-PreS-O), combining RBDs from Wuhan hu-1 wild-type and Omicron BA.1 with the hepatitis B virus (HBV)-derived PreS surface antigen adsorbed to aluminum hydroxide. …”
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First Measurements of Ambient PM2.5 in Kinshasa, Democratic Republic of Congo and Brazzaville, Republic of Congo Using Field-calibrated Low-cost Sensors by Celeste McFarlane, Paulson Kasereka Isevulambire, Raymond Sinsi Lumbuenamo, Arnold Murphy Elouma Ndinga, Ranil Dhammapala, Xiaomeng Jin, V. Faye McNeill, Carl Malings, R. Subramanian, Daniel M. Westervelt

“…Finally, the surface PM2.5 level and the aerosol optical depth were about 40% lower during the COVID-19 lockdown in 2020 than the corresponding period in 2019, which cannot be attributed solely to changes in meteorology or wildfire emission. …”
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Mis-localization of endogenous TDP-43 leads to ALS-like early-stage metabolic dysfunction and progressive motor deficits by Yiying Hu, Alexander Hruscha, Chenchen Pan, Martina Schifferer, Michael K. Schmidt, Brigitte Nuscher, Martin Giera, Sarantos Kostidis, Özge Burhan, Frauke van Bebber, Dieter Edbauer, Thomas Arzberger, Christian Haass, Bettina Schmid

“…Taking advantage of zebrafish’s embryonic development that solely relys on yolk usage until 5 days post fertilization, we demonstrated that microglia proliferation and activation in the hypothalamus is independent from food intake. …”
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The role of mycobacteremia screening in enhancing non-tuberculous mycobacteria detection in hospitalized persons with HIV by Mengjiao Miao, Hongyan Zhu, Ziyao Liu, Jinjin Yang, Yiting Zhang, Chenyu Ma, Jiamin Qin, Yaling Chen, Hongxia Wei, Wei Chen, Yongfeng Yang, Zhiliang Hu, Zhiliang Hu, Zhiliang Hu

“…Notably, mycobacteremia served as the sole microbiological evidence in approximately 25% of all culture-proven NTM infections during initial hospitalization, where other specimen sources failed to yield conclusive evidence. …”
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Determination and prediction of the apparent and standardized ileal amino acid digestibility in soybean meal fed to the pullets by Sumei Cao, Yuxin Yang, Guangtian Ma, Jing Wang, Kai Qiu, Zhigang Song, Shugeng Wu, Guanghai Qi, Haijun Zhang

“…This experiment was conducted to evaluate the chemical composition, apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of amino acid (AA) in 10 different sources of soybean meal (SBM) fed to the pullets and established prediction equations based on the chemical composition of SBM. 792 Hy Line Brown hens aged 12-15 weeks were divided into 11 diet groups, the diets included one nitrogen-free diet (NFD) used to measure the basal endogenous losses of AA and 10 SBM test diets containing 35.2 % SBM as the sole source of AA. The 0.5 % titanium dioxide (TiO2) was used as an indigestible marker. …”
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Modified sutureless and glue-free method versus conventional sutures for conjunctival autograft fixation in primary pterygium surgery: a systematic review and meta-analysis by Faisal Aljahdali, Waleed Khayyat, Abdulelah T BinYamin, Sultan A Al-Qahtani, Mohammed D Alghamdi, Ali Saleh Alsudais, Husain A Alalgum, Halah Bin Helayel, Mohammed AlMutlak

“…No significant differences were found in recurrence, graft haemorrhage, granuloma, Dellen and conjunctival oedema.Conclusion Using the MSGF technique in conjunctival autograft fixation for pterygium surgery reduces operation time by relying solely on the patient’s blood for fixation. However, it increases the risk of graft dehiscence and retraction. …”
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Predictors of Cardiovascular Autonomic Neuropathy Onset and Progression in a Cohort of Type 1 Diabetic Patients by M. Matta, A. Pavy-Le Traon, S. Perez-Lloret, C. Laporte, I. Berdugo, N. Nasr, H. Hanaire, J. M. Senard

“…A multivariate analysis indicated that lower BMI (OR: 0.15, CI 95%: 0.05–0.48, p=0.003) and SSRI exposure (OR: 4.18, CI 95%: 1.03–16.97, p=0.04) were the sole predictors of CAN deterioration. In the 55 patients negative for CAN at the first laboratory assessment, 12 became positive at the second assessment. …”
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Optimized extraction and kinetic study of cholesterol oxidase from a newly isolated Escherichia fergusonii strain from local whey samples: insights through a combined experimental... by Simin Khataee, Gholamreza Dehghan, Samaneh Rashtbari, Arezu Marefat, Sina Jamei, Hamed Farzi-Khajeh

“…The maximum production of ChoX was obtained at the optimum condition of 48 h of incubation under shaking conditions (130 rpm) at 35 °C in a basal medium adjusted to pH 6.5, including 1.4 g/L cholesterol as a sole carbon. The crude product was purified by ammonium sulfate precipitation and followed by ion exchange chromatography utilizing Q-Sepharose, resulting in 5.35-fold and 13.86-fold purification, respectively, with a final specific activity of 15.8 U/mg. …”
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Brood thermoregulation effectiveness is positively linked to the amount of brood but not to the number of bees in honeybee colonies by Godeau, Ugoline, Pioz, Maryline, Martin, Olivier, Rüger, Charlotte, Crauser, Didier, Le Conte, Yves, Henry, Mickael, Alaux, Cédric

“…The relationship between brood amount and mean temperature was however too weak for clearly discriminating colony population size based solely on the brood thermoregulatory effectiveness. …”
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Identification of progressive pulmonary fibrosis: consensus findings from a modified Delphi study by Athol U. Wells, Simon L. F. Walsh, Ayodeji Adegunsoye, Vincent Cottin, Sonye K. Danoff, Anand Devaraj, Kevin R. Flaherty, Peter M. George, Kerri A. Johannson, Martin Kolb, Yasuhiro Kondoh, Andrew G. Nicholson, Sara Tomassetti, Elizabeth R. Volkmann, Kevin K. Brown

“…Consensus was reached that a histological pattern of usual interstitial pneumonia (UIP) is a risk factor for progression of ILD, but that a biopsy to look for a UIP pattern should not be performed solely for prognostic reasons. Consensus was not reached on the time period over which progression should be defined. …”
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Machine learning reveals sex differences in distinguishing between conduct-disordered and neurotypical youth based on emotion processing dysfunction by Gregor Kohls, Erik M. Elster, Peter Tino, Graeme Fairchild, Christina Stadler, Arne Popma, Christine M. Freitag, Stephane A. De Brito, Kerstin Konrad, Ruth Pauli

“…Results Both female and male ML classifiers accurately predicted (above chance level) individual CD status based solely on the neurocognitive features of emotion dysfunction. …”
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Evolution within a given virulence phenotype (pathotype) is driven by changes in aggressiveness: a case study of French wheat leaf rust populations by Fontyn, Cécilia, Meyer, Kevin JG, Boixel, Anne-Lise, Delestre, Ghislain, Piaget, Emma, Picard, Corentin, Suffert, Frédéric, Marcel, Thierry C, Goyeau, Henriette

“…The relative dynamics and the domination of these two pathotypes — 166 317 0 and 106 314 0 —, relative to the other pathotypes present in the population at a low frequency although compatible, i.e. virulent on several varieties deployed, could not be explained solely by the frequency of Lr genes in the landscape. …”
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Late-stage diversification strategy for the synthesis of peptide acids and amides using hydrazides by Shoko Tanaka, Mizuki Kanno, Yosuke Tashiro, Tetsuo Narumi, Nobuyuki Mase, Kohei Sato

“…Methods: Peptide hydrazides were synthesized solely by using conventional solid-phase peptide synthesis (SPPS). …”
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Evaluating the Sustainability of Wheat–Maize System with a Long-Term Fertilization Experiment by Yun Shao, Jiahui An, Xueping Wang, Shouchen Ma, Ye Meng, Yang Gao, Shoutian Ma

“…Through an assessment of the soil system’s sustainability under various treatments, it becomes evident that following a two-year cessation of fertilization, the sustainability indexes of the soils subjected to three long-term organic + inorganic fertilizer treatments (1.26, 1.29, and 1.27) exceeded that of the soil treated solely with chemical fertilizer (1.00). These findings provide further evidence supporting the notion that the combined application of organic and inorganic fertilizers can enhance the soil system’s capacity for sustainable production in wheat–maize farmland within the Huang-Huai-Hai Plain.…”
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Relevance of superoxide dismutase type 1 to lipoid pneumonia: the first retrospective case-control study by Yinan Hu, Yanhong Ren, Yinzhen Han, Zhen Li, Weiqing Meng, Yuhui Qiang, Mengyuan Liu, Huaping Dai

“…Results In multivariate regression analysis, the sole independent risk factor of LP was the level of SOD1 (OR 0.922, 95% CI: 0.878 ~ 0.967, P < 0.001), while smoking status (β= -0.177, 95% CI -18.645~-2.836, P = 0.008), diabetes mellitus (β= -0.191, 95% CI: -20.442~-3.592, P = 0.005), and total sialic acid (TSA) (β= -0.426, 95% CI: -0.915~ -0.433, P < 0.001) independently influenced the level of SOD1. …”
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Synergistic effects of allantoin and Achyranthes japonica-biochar profoundly alleviate lead toxicity during barley growth by Ghulam Murtaza, Najmaldin Ezaldin Hassan, Muhammad Usman, Gang Deng, Zeeshan Ahmed, Javed Iqbal, Mohamed S. Elshikh, Humaira Rizwana, Basharat Ali, Rashid Iqbal, Maximilian Lackner

“…The resistance towards Pb2+ was enhanced by biochar, allantoin, and their combined application, with lower Pb2+ concentrations in shoots (59.9 %, 40.1 %, and 49.8 %), roots (48.2 %, 24.1 %, and 58.3 %), and grains (60.2 %, 29.7 %, and 40.1 %) compared to solely Pb-stress, respectively. In summary, converting the weed Achyranthes japonica into biochar and integrating it with allantoin provides an eco-friendly approach to control its proliferation while efficiently alleviating Pb-induced toxicity in plants.…”
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Magnitude and dynamics of the T-cell response to SARS-CoV-2 infection at both individual and population levels by Thomas M. Snyder, Rachel M. Gittelman, Mark Klinger, Damon H. May, Edward J. Osborne, Ruth Taniguchi, H. Jabran Zahid, Ian M. Kaplan, Jennifer N. Dines, Matthew T. Noakes, Ravi Pandya, Xiaoyu Chen, Summer Elasady, Emily Svejnoha, Peter Ebert, Mitchell W. Pesesky, Patricia De Almeida, Hope O’Donnell, Quinn DeGottardi, Gladys Keitany, Jennifer Lu, Allen Vong, Rebecca Elyanow, Paul Fields, Hussein Al-Asadi, Julia Greissl, Lance Baldo, Simona Semprini, Claudio Cerchione, Fabio Nicolini, Massimiliano Mazza, Ottavia M. Delmonte, Kerry Dobbs, Rocio Laguna-Goya, Gonzalo Carreño-Tarragona, Santiago Barrio, Luisa Imberti, Alessandra Sottini, Eugenia Quiros-Roldan, Camillo Rossi, Andrea Biondi, Laura Rachele Bettini, Mariella D’Angio, Paolo Bonfanti, Miranda F. Tompkins, Camille Alba, Clifton Dalgard, Vittorio Sambri, Giovanni Martinelli, Jason D. Goldman, Jason D. Goldman, James R. Heath, Helen C. Su, Luigi D. Notarangelo, Estela Paz-Artal, Joaquin Martinez-Lopez, Bryan Howie, Jonathan M. Carlson, Harlan S. Robins

“…As an application of these data, we trained a classifier to diagnose SARS-CoV-2 infection based solely on TCR sequencing from blood samples, and observed, at 99.8% specificity, high early sensitivity soon after diagnosis (Day 3–7 = 85.1% [95% CI = 79.9–89.7]; Day 8–14 = 94.8% [90.7–98.4]) as well as lasting sensitivity after recovery (Day 29+/convalescent = 95.4% [92.1–98.3]).DiscussionThe approaches described in this work provide detailed insights into the adaptive immune response to SARS-CoV-2 infection, and they have potential applications in clinical diagnostics, vaccine development, and monitoring.…”
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Combined effects of alginate based active edible coatings and irradiation treatment on the quality characteristics of Beef Meat at 2°C by Samir Smeti, Souha Tibaoui, Hayet Ben Haj Koubaier, Atef Lakoud, Naziha Atti

“…These treatments were compared to those of irradiation treatment solely or combined with the edible films. Therefore, six treatments were developed: uncoated samples (CON); samples with edible coating (AEC); samples with edible coating enriched with 200 ppm of rosemary extract (ROS); samples with edible coating enriched with 200 ppm of myrtle extract (MYRT); irradiated samples (IRR); and irradiated samples with edible coating (IRAC). …”
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Predictive models for the implementation of targeted reproductive management in multiparous cows on automatic milking systems by Fergus P. Hannon, Martin J. Green, Luke O'Grady, Chris Hudson, Anneke Gouw, Laura V. Randall

“…Our second objective was to assess the change in model performance following the addition of 2 auxiliary data sources. Using data derived solely from the AMS (RBT dataset) a binary random forest classification model was constructed for both outcomes of interest. …”
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Does the CDC Surgical Wound Classification adequately predict postoperative infection in lower extremity fracture surgery? by Elizabeth Cho, MD, Hanna House, MD, Andrew Marten, BS, Marina Feffer, MPH, Julie Agel, MA, John Scolaro, MD, Meir Marmor, MD, Ashley E. Levack, MD, MAS, OTA Classification & Outcomes Committee, James Kellam, MD, Gillian Soles, MD, Jarrod Dumpe, MD, Kyle Schweser, MD, Geoffrey Maracek, MD

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