Interconvertible lac repressor-DNA loops revealed by single-molecule experiments. by Oi Kwan Wong, Martin Guthold, Dorothy A Erie, Jeff Gelles

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IUPAB and BSJ meeting Kyoto: Reflections on hands on workshop “Real-time single-molecule experiments with optical tweezers and correlated fluorescence microscopy” with LUMICKS C-trap, emphasizing the importance of practicing international and interdisciplinary science by Karina New

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Arrest Peptide Profiling resolves co-translational folding pathways and chaperone interactions in vivo by Xiuqi Chen, Vincent J. Hilser, Christian M. Kaiser

“…Combining AP Profiling with single-molecule experiments, we delineate co-translational folding for a set of GTPase domains with similar structures, defining how topology shapes folding pathways. …”
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High-throughput single-molecule quantification of individual base stacking energies in nucleic acids by Jibin Abraham Punnoose, Kevin J. Thomas, Arun Richard Chandrasekaran, Javier Vilcapoma, Andrew Hayden, Kacey Kilpatrick, Sweta Vangaveti, Alan Chen, Thomas Banco, Ken Halvorsen

“…Here, we use a Centrifuge Force Microscope for high-throughput single molecule experiments to measure stacking energies between adjacent bases. …”
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The YoaA-χ helicase modulates the dynamics of single-stranded DNA binding protein on DNA by Savannah J. Weeks-Pollenz, Matthew J. Petrides, Kaylie A. Padgett-Pagliai, Robert Davis, Kathryn K. Harris, Linda B. Bloom

“…SSB similarly affects the activity of the YoaA paralog, DinG. Single-molecule experiments show that SSB translocates with YoaA-χ, increasing both the lifetime and frequency of SSB binding events. …”
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Quantifying and optimizing single-molecule switching nanoscopy at high speeds. by Yu Lin, Jane J Long, Fang Huang, Whitney C Duim, Stefanie Kirschbaum, Yongdeng Zhang, Lena K Schroeder, Aleksander A Rebane, Mary Grace M Velasco, Alejandro Virrueta, Daniel W Moonan, Junyi Jiao, Sandy Y Hernandez, Yongli Zhang, Joerg Bewersdorf

“…Furthermore, we measured the photoswitching kinetics of Alexa Fluor 647 from single-molecule experiments, and, based on this kinetic data, we developed algorithms to simulate single-molecule switching nanoscopy images. …”
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Coupling of lever arm swing and biased Brownian motion in actomyosin. by Qing-Miao Nie, Akio Togashi, Takeshi N Sasaki, Mitsunori Takano, Masaki Sasai, Tomoki P Terada

“…An alternative hypothesis, the Brownian motion hypothesis, has been supported by single-molecule experiments and emphasizes more on the roles of fluctuating protein movement. …”
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Hydrophobic fluorescent probes introduce artifacts into single molecule tracking experiments due to non-specific binding. by Laura C Zanetti-Domingues, Christopher J Tynan, Daniel J Rolfe, David T Clarke, Marisa Martin-Fernandez

“…We conclude that the physic-chemical properties of the dyes should be considered carefully when planning single-molecule experiments. Favourable dye characteristics such as photostability and brightness can be offset by the propensity of a conjugate for non-specific adhesion.…”
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Proline isomerization modulates the bacterial IsdB/hemoglobin interaction: an atomic force spectroscopy study by Francesca Pancrazi, Omar De Bei, Francesco Lavecchia di Tocco, Marialaura Marchetti, Barbara Campanini, Salvatore Cannistraro, Stefano Bettati, Anna Rita Bizzarri

“…Abstract Iron surface determinant B (IsdB), a Staphylococcus aureus (SA) surface protein involved in both heme iron acquisition from host hemoglobin (Hb) and bacterial adhesion, is a proven virulence factor that can be targeted for the design of antibacterial molecules or vaccines. Recent single-molecule experiments on IsdB interaction with cell adhesion factors revealed an increase of the complex lifetime upon applying a stronger force (catch bond); this was suggested to favor host invasion under shear stress. …”
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Plasticity and Co-Factor-Dependent Structural Changes in the RecA Nucleoprotein Filament Studied by Small-Angle X-Ray Scattering (SAXS) Measurements and Molecular Modeling by Satomi Inaba-Inoue, Afra Sabei, Anne-Elisabeth Molza, Mara Prentiss, Tsutomu Mikawa, Hiroshi Sekiguchi, Chantal Prévost, Masayuki Takahashi

“…Cooperative transition from one form to the other has been observed during single-molecule experiments by pulling on the filament but also in solution by modifying solvent conditions. …”
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