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    Duplex qPCR for detecting and differentiating porcine epidemic diarrhea virus GI and GII subtypes by Bin Wang, Wang Han, Di Wu, Yue Jing, Li Ma, Feiyang Jiang, Shusen Ji, Lianmei Bai, Xiuju Yu, Wei Hou, Shouyu Wang, Haidong Wang

    Published 2025-01-01
    “…PCR is the most commonly used technique for PEDV diagnosis in practical clinics, however, reported works still suffer from shortcomings, for example, most of them cannot differentiate GI and GII subtypes, they suffer from low sensitivity, and some primer sequences are no longer able to match the mutant strains.MethodsTo address these issues, we conducted a comprehensive analysis by comparing the sequences of the PEDV S protein in the existing NCBI database with a recently isolated epidemic strain of PEDV, named SX0818-2022, of subtype GIIa from Shanxi, China. The conserved sequences of GI and GII subtypes were retrieved to design the primers and probe. …”
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    The Effect of Weed Control with Pre-Emergence Herbicides on the Yield Level of Mung Bean Yield by Cailing Jing, Junying Wang, Yonghong Wu, Yufan Zhou, Huijun Zhu, Yaowen Zhang, Shuqi Dong, Xiaorui Li, Juan Zhao, Junli Cao, Xiangyang Yuan, Xi’e Song

    Published 2025-01-01
    “…The purpose of this study is to screen out safe and efficient pre-emergence herbicides suitable for the Shanxi mung bean, which is of great significance due to its large-scale planting industrialization and the development of advantageous industries.…”
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