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    Aggregation of ice-nucleating macromolecules from <i>Betula pendula</i> pollen determines ice nucleation efficiency by F. Wieland, F. Wieland, N. Bothen, R. Schwidetzky, T. M. Seifried, T. M. Seifried, P. Bieber, P. Bieber, U. Pöschl, K. Meister, K. Meister, M. Bonn, J. Fröhlich-Nowoisky, H. Grothe

    Published 2025-01-01
    “…Our study reveals that three distinct INM classes active at <span class="inline-formula">−</span>8.7, <span class="inline-formula">−</span>15.7, and <span class="inline-formula">−</span>17.4 °C are present in <i>B. pendula</i>. Freeze drying and freeze–thaw cycles noticeably alter their ice nucleation capability, and the results of heat treatment, size, and chemical analysis indicate that INM classes correspond to size-varying aggregates, with larger aggregates nucleating ice at higher temperatures, in agreement with previous studies on fungal and bacterial ice nucleators. …”
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