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    Sample Aging Profoundly Reduces Monocyte Responses in Human Whole Blood Cultures by H. W. Grievink, M. Moerland

    Published 2018-01-01
    “…The reduction ranged from 20–50% after 30 minutes to 80–100% after 10 hours and differed between cytokines (IL-1β, IL-2, IL-6, IFNγ, and TNFα). Sample storage at 4°C or 37°C even worsened this. PMA/ionomycin- and SEB-induced cytokine release, both mainly T-cell-driven, were also reduced by sample aging but to a lesser extent (20–50% after 24 hours). …”
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