Acute Liver Failure Caused by Amanita phalloides Poisoning by Luca Santi, Caterina Maggioli, Marianna Mastroroberto, Manuel Tufoni, Lucia Napoli, Paolo Caraceni

“…Liver damage from Amanita phalloides is related to the amanitins, powerful toxins that inhibit RNA polymerase II resulting in a deficient protein synthesis and cell necrosis. …”
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Rapid Characterization and Action Mechanism of the Antidiabetic Effect of Diospyros lotus L Using UHPLC-Q-Exactive Orbitrap MS and Network Pharmacology by Shihan Qin, Mingjuan Liu, Sunv Tang, E. Shuai, Ziming Wang, Kaiquan Yu, Wei Cai

“…In addition, 92 relevant antidiabetic targets were mainly related to positive regulation of transcription from the RNA polymerase II promoter, extracellular space, and protein binding, suggesting the involvement of TNF, PI3K-Akt, and HIF-1 signaling pathways in the antidiabetic effect of Diospyros lotus L. …”
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Study of some virulence genes from uropathogenic Klebsiella pneumoniae and Pseudomonas aeruginosa isolated in Wasit Province, Iraq by Rana H. Raheema, Sabaa Hilal Hadi, Zainab Adil Ghani Chabuck

“…These findings were validated by the polymerase chain reaction method used to detect the virulence genes fimH gene, where 4/16 (25%) and mrkA gene, where result 7/16 (43.75%) were positive in recovered K. pneumoniae isolated. And RNA polymerase subunit B (rpoB) gene where results 10/13 (76.92%) were positive in recovered P. aeruginosa isolated. …”
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The role of SAGA in the transcription and export of mRNA by E. N. Nabirochkina, M. M. Kurshakova, S. G. Georgieva, D. V. Kopytova

“…We also revealed that Sgf11 was present at many active transcription sites in interbands and puffs on polytene chromosomes, Sgf11 was localized at all Brf1 (the component of the RNA polymerase III basal transcription complex) sites. …”
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Morpho-phylogenetic evidence reveals novel hyphomycetous fungi on medicinal plants in Southwestern China by Hong-Zhi Du, Ning-Guo Liu, Na Wu, Ratchadawan Cheewangkoon, Jian-Kui Liu

“…These taxa were characterised and identified based on morphological and culture characteristics, coupled with phylogenetic analyses of combined sequences of calmodulin (cmdA), the internal transcribed spacer region of ribosomal DNA (ITS), nuclear large subunit ribosomal DNA (LSU), RNA polymerase second-largest subunit (rpb2), the translation elongation factor 1-alpha (tef1-α), and β-tubulin (tub2). …”
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Rhipicephalus simus ticks: new hosts for phleboviruses by Samuel Munalula Munjita, Benjamin Mubemba, John Tembo, Mathew Bates, Sody Munsaka, Ala Tabor

“…Its RNA-dependent RNA polymerase (RdRp) encoding region carried conserved RdRp and endonuclease domains characteristic of phenuiviridae viruses. …”
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Trichoderma collection from Brazilian soil reveals a new species: T. cerradensis Sp. nov. by Gustavo Henrique Silva Peixoto, Rildo Alexandre Fernandes da Silva, Ana Beatriz Zacaroni, Thais França Silva, Priscila Chaverri, Priscila Chaverri, Danilo Batista Pinho, Sueli Corrêa Marques de Mello

“…In this study, 97 Trichoderma isolates preserved at the EMBRAPA collection (an important source for biocontrol agents) and previously collected from several areas in Brazil were characterized which were identified using various molecular markers (internal transcribed spacers (its), translation elongation factor (tef1α), RNA polymerase II subunit (rpb2), actin (act), and calmodulin (cal). …”
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Investigating the reassortment potential and pathogenicity of the S segment in Akabane virus using a reverse genetics system by Eun-Jee Na, Chang-Gi Jeong, Su-Beom Chae, Jae-Ku Oem

“…In this study, changes in viral replication and pathogenicity were investigated, particularly when the S segment of AKAV-7 was mutated using a T7 RNA polymerase-based reverse genetics (RG) system. Results The rAKAV-7ΔNSs virus, with a deletion in the NSs protein of the wild-type AKAV-7 virus (wtAKAV-7), and the rAKAV-7(S-K0505) virus, where the S segment of wtAKAV-7 was reassorted with that from the wild type K0505 strain (wtK0505), were successfully rescued. …”
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Multilocus phylogenies reveal three new truffle-like taxa and the traces of interspecific hybridization in Octaviania (Boletaceae, Boletales) by Takamichi Orihara, Rosanne Healy, Adriana Corrales, Matthew E. Smith

“…Here we describe two new species in subgenus Octaviania, O. tenuipes and O. tomentosa, from temperate to subtropical evergreen Fagaceae forests in Japan based on morphological observation and robust multilocus phylogenetic analyses (nrDNA ITS and partial large subunit [LSU], translation elongation factor 1-α gene [TEF1] and the largest subunit of RNA polymerase II gene [RPB1]). Based on specimens from the Americas as well as studies of the holotype, we also taxonomically re-evaluate O. asterosperma var. potteri. …”
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Replication-transcription complex of coronaviruses: functions of individual viral non-structural subunits, properties and architecture of their complexes by E. L. Mishchenko, V. A. Ivanisenko

“…The enzyme targets include papain- and 3C-like cysteine proteinases that process two large viral polyproteins, RNA-dependent RNA polymerase, RNA helicase, viral genome-modifying enzymes, and enzymes with 3’–5’ exoribonuclease or uridylate-specif ic endonuclease activity. …”
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Impact of R-loops on oncogene-induced replication stress in cancer cells by Heuzé, Jonathan, Lin, Yea-Lih, Lengronne, Armelle, Poli, Jérôme, Pasero, Philippe

“…These structures form during transcription when nascent RNA reanneals with DNA behind the RNA polymerase, forming a stable DNA:RNA hybrid. In this review, we discuss how these different cotranscriptional processes disrupt the progression of replication forks and how they contribute to genetic instability in cancer cells.…”
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Re-identification of Strains from Aspergillus Section Aspergillus and Description of Three Unrecorded Species from Korea by Anbazhagan Mageswari, Daseul Lee, Le Dinh Thao, Donghun Kang, Dong-Hyun Kim, Seung-Beom Hong

“…In this study, 108 strains from the section Aspergillus, originating from Korea and conserved at the Korean Agricultural Culture Collection, were subjected to re-identification using a combined dataset that included partial sequences of β-tubulin (BenA), Calmodulin (CaM), and RNA polymerase II second largest subunit (RPB2) genes, along with their morphological characteristics. …”
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Molecular reassessment of diaporthalean fungi associated with strawberry, including the leaf blight fungus, Paraphomopsis obscurans gen. et comb. nov. (Melanconiellaceae) by Dhanushka Udayanga, Shaneya D. Miriyagalla, Dimuthu S. Manamgoda, Kim S. Lewers, Alain Gardiennet, Lisa A. Castlebury

“…Combined analyses of four nuclear loci, 28S ribosomal DNA/large subunit rDNA (LSU), ribosomal internal transcribed spacers 1 and 2 with 5.8S ribosomal DNA (ITS), partial sequences of second largest subunit of RNA polymerase II (RPB2) and translation elongation factor 1-α (TEF1), were used to reconstruct a phylogeny for these pathogens. …”
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Genomic Characterization of <i>Laodelphax striatellus</i> Permutotetra-like Virus and Self-Cleavage Function of Viral Capsid Protein by Jun Piao, Jiarui Zhang, Lujie Zhang, Jingai Piao, Haitao Wang, Yilin Xie, Shuo Li

“…It was found that the LsPLV genome was 4667 nt in length, encoding two proteins, RNA-dependent RNA polymerase (RdRP) and CP, and the palm subdomain conserved region in RdRp was arranged in a “C–A–B” permutation pattern, exhibiting the typical characteristics of permutotetra-like viruses. …”
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TEFM facilitates transition from RNA synthesis to DNA synthesis at H-strand replication origin of mtDNA by Shigeru Matsuda, Masunari Nakayama, Yura Do, Takashi Ishiuchi, Mikako Yagi, Sjoerd Wanrooij, Kazuto Nakada, Fan-Yan Wei, Kenji Ichiyanagi, Hiroyuki Sasaki, Dongchon Kang, Takehiro Yasukawa

“…Taken together, we propose that, in addition to conferring processivity to the mitochondrial RNA polymerase, TEFM plays a crucial role in maintaining the balance between mitochondrial transcription and replication.…”
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PCR-based detection of Botryosphaeria canker pathogens in fig trees by Mahdiyeh Ghaedi, Zeinab Bolboli, Hamed Negahban, Reza Mostowfizadeh-Ghalamfarsa

“…In our study, we developed a PCR assay using three carefully selected primer pairs based on sequence differences in two protein-coding genes, β-tubulin and RNA polymerase II. The species-specific primers TUB-Bd1 (specific for Botryosphaeria dothidea), TUB-Np1 (specific for Neofusicoccum parvum), and RPB-Nd2 (specific for Neoscytalidium dimidiatum) effectively amplified target gene sequences in pure cultures and infected tissue samples using PCR and nested PCR conditions. …”
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Different functions of PHF10 isoforms – subunits of the PBAF chromatin remodeling complex by A. A. Sheynov, V. V. Tatarskiy, A. M. Azieva, S. G. Georgieva, N. V. Soshnikova

“…These isoforms contain PHD (plant homeodomain) fingers for nucleosome binding and recruit RNA polymerase II on the promoters of cell cycle genes. …”
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New insights into the stipitate hydnoid fungi Sarcodon, Hydnellum, and the formerly informally defined Neosarcodon, with emphasis on the edible species marketed in Southwest China by Di Wang, Hui Feng, Jie Zhou, Tian-Hai Liu, Zhi-Yuan Zhang, Ying-Yin Xu, Jie Tang, Wei-Hong Peng, Xiao-Lan He

“…Phylogeny of the traditional Sarcodon s. lat. and Hydnellum s. lat. was reconstructed from the combined internal transcribed spacer (ITS), nuclear large ribosomal subunit (nLSU) and RNA polymerase II second largest subunit (RPB2) dataset based on expanded samples to reevaluate the taxonomic placements of the two genera. …”
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The Molecular Diagnosis Protocols of New Coronavirus (COVID-19); Specificity and Sensitivity an Overview by Abdullah Ahmed Hama, Othman Abdulrahman Mohammed, Fatima Mahmud Ali, Osama Hamid Shareef, Sardar Muhammad Wli, Sabiha Sharif, Syamand Ahmed Qadir

“…In conclusion of this review, the RNA dependent RNA polymerase (RdRp) and RdRp/Hel protocols will be valuable to distinguish the  COVID-19 from the SARS-CoV and the other respiratory viral pathogens. …”
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Analysis of bacterial genetic diversity in biofloc by using ARDRA 16S-rRNA gene by , Widanarni, Dewi Nurhayati, Dinamella Wahjuningrum

“…ABSTRACT   This study aimed to analyze the genetic diversity of bacteria associated in bioflocs using 16S-rRNA polymerase chain reaction (PCR) with ARDRA technique. …”
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