Two novel species of Aspergillus section Nigri from indoor air by Željko Jurjević, Stephen W. Peterson, Gaetano Stea, Michele Solfrizzo, János Varga, Vit Hubka, Giancarlo Perrone

“…To describe the species we used phenotypes from 7-d Czapek yeast extract agar culture (CYA), creatine agar culture (CREA) and malt extract agar culture (MEA), with support by molecular analysis of the β-tubulin, calmodulin, RNA polymerase II (RPB2), and translation elongation factor-alpha (TEF) gene amplified and sequenced from 56 air isolates and one isolate from almonds belonging to Aspergillus section Nigri. …”
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Artificial cells with all-aqueous droplet-in-droplet structures for spatially separated transcription and translation by Kanji Tomohara, Yoshihiro Minagawa, Hiroyuki Noji

“…The inner droplet is designed to enrich DNA and RNA polymerase for transcription, coupled to translation at the outer droplet via mRNA-mediated cascade reactions. …”
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R-Loop Formation In Trans at an AGGAG Repeat by Kazuya Toriumi, Takuma Tsukahara, Ryo Hanai

“…Formation of RNA-DNA hybrid, or R-loop, was studied in vitro by transcribing an AGGAG repeat with T7 RNA polymerase. When ribonuclease T1 was present, R-loop formation in cis was diminished, indicating that the transcript was separated from the template and reassociated with it. …”
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Aspergillus section Versicolores: nine new species and multilocus DNA sequence based phylogeny by Z. Jurjevic, S.W. Peterson, B.W. Horn

“…β-tubulin, calmodulin, internal transcribed spacer and partial lsu-rDNA, RNA polymerase 2, DNA replication licensing factor Mcm7, and pre-rRNA processing protein Tsr1 were amplified and sequenced from numerous isolates belonging to Aspergillus sect. versicolor. …”
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In Vitro Comparison of the Internal Ribosomal Entry Site Activity from Rodent Hepacivirus and Pegivirus and Construction of Pseudoparticles by Stuart Sims, Kevin Michaelsen, Sara Burkhard, Cornel Fraefel

“…Utilizing a monocistronic expression vector with an RNA polymerase I promoter to drive transcription, we show cell-specific IRES translation and regions within the IRES required for full functionality. …”
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A Novel Type of Non-coding RNA, nc886, Implicated in Tumor Sensing and Suppression by Yong Sun Lee

“…nc886 (=vtRNA2-1, pre-miR-886, or CBL3) is a newly identified non-coding RNA (ncRNA) that represses the activity of protein kinase R (PKR). nc886 is transcribed by RNA polymerase III (Pol III) and is intriguingly the first case of a Pol III gene whose expression is silenced by CpG DNA hypermethylation in several types of cancer. …”
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Effects of Carbonate Alkalinity on Antioxidants, Immunity and Intestinal Flora of <i>Penaeus vannamei</i> by Chuang Gao, Zhonghong Li, Bo Ma, Rantao Zuo, Xudong Shen, Mingkang Chen, Chuanqi Ren, Wenjun Zheng, Zhonglu Cai, Jiaxing Li, Shuang Li

“…The functional prediction results of COG and KEGG showed that the functional abundance of RNA polymerase sigma-70 factor is direct bacterial or plastid core RNA polymerase and is specific to promoter elements that are situated 10 and 35 base-pairs upstream of transcription-initiation points—in the high carbonate alkalinity treatment group, this was higher than that in the control group. …”
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New members of Alternaria (Pleosporales, Pleosporaceae) collected from Apiaceae in Algeria by Nabahat Bessadat, Nelly Bataillé-Simoneau, Justine Colou, Bruno Hamon, Kihal Mabrouk, Philippe Simoneau

“…Phylogenetic analysis using sequences at four loci, the internal transcribed spacer of the rDNA region (ITS), glyceraldehyde-3-phosphate dehydrogenase (gpd), translation elongation factor 1-alpha (tef1), and RNA polymerase second largest subunit (rpb2), revealed that these isolates grouped into three sections, namely Embellisia, Embellisioides, and Eureka. …”
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A contribution to Porogramme (Polyporaceae, Agaricomycetes) and related genera by Wei-Lin Mao, Ying-Da Wu, Hong-Gao Liu, Yuan Yuan, Yu-Cheng Dai

“…Our molecular phylogeny based on the internal transcribed spacer (ITS), the large subunit nuclear ribosomal RNA gene (nLSU), the translation elongation factor 1-α gene (TEF1), and the largest subunit of RNA polymerase II (RPB1) demonstrates six clades are formed among Porogramme and related genera. …”
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Morpho-phylogenetic analyses of two novel edible mushrooms from China and a mini review of Lyophyllum (Agaricales, Lyophyllaceae) cultivation and bioactivities by Song-Ming Tang, Feng-Ming Yu, Samantha C. Karunarathna, Zong-Long Luo, Kai-Yang Niu, Rui-Yu Li, Lin Li, Xi-Jun Su, Shu-Hong Li

“…Molecular phylogenetic analyses using the internal transcribed spacer ITS1-5.8S-ITS2 ribosomal RNA (ITS), the large subunit ribosomal RNA (LSU), the second-largest subunit of RNA polymerase II (rpb2), and translation elongation factor 1-alpha (tef1-α) indicated that L. edulis is related to L. shimeji, L. heimogu, and L. decastes, and L. sinense has an affinity to L. bulborhizum and L. nigrum. …”
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Understanding Rifampicin Resistance in Tuberculosis through a Computational Approach by Satish Kumar, Lingaraja Jena

“…Mutation in rpoB, the beta subunit of DNA-directed RNA polymerase of MTB, is reported to be a major cause of RIF resistance. …”
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Molecular Phylogenetics and Estimation of Evolutionary Divergence and Biogeography of the Family Cordycipitaceae (Ascomycota, Hypocreales) by Huili Pu, Jiao Yang, Nemat O. Keyhani, Lixia Yang, Minhai Zheng, Chenghao Qiu, Yuchen Mao, Junya Shang, Yongsheng Lin, Chengjie Xiong, Longbing Lin, Pengyu Lai, Yanbin Huang, Xin Yuan, Huiling Liang, Longfei Fan, Xiaoli Ma, Chunjin Qiu, Junzhi Qiu

“…Based on Bayesian evolutionary analysis of five genomic loci(the small subunit of ribosomal RNA (SSU) gene, the large subunit of ribosomal RNA (LSU) gene, the translation elongation factor 1-α (<i>tef1-α</i>) gene, the largest subunit of RNA polymerase II (<i>rpb1</i>), and the second largest subunit of RNA polymerase II (<i>rpb2</i>), we inferred the divergence times for members of the Cordycipitaceae, improving the internal phylogeny of this fungal family. …”
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Investigating Pb2 CAP-binding domain inhibitors from marine bacteria for targeting the influenza A H5N1. by Taha A Kumosani, Aymn T Abbas, Balogun Basheer, Ahmed M Hassan, Soonham S Yaghmoor, Areej H Alyahiby, Amer H Asseri, Vivek Dhar Dwivedi, Esam I Azhar

“…A promising strategy for antiviral drug development involves targeting the RNA-dependent RNA polymerase, specifically the PB2-cap binding domain of Influenza A H5N1. …”
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Homology Modeling and Expression of Recombinant NS5-RdRp Based on the Indonesian Local Strain of Dengue Virus for Anti-Dengue Drug Development by Toto Subroto, Ade Rizqi Ridwan Firdaus, Shinta Kusumawardani, Taufik Ramdani Tohari, Theresia Adventina Nababan, Wulan Pertiwi, Muhammad Fadhlillah, Sri Agung Fitri Kusuma, Yaya Rukayadi

“… This study aims to isolate the RNA-dependent RNA polymerase (RdRp) gene of the dengue virus (DENV) isolates from Indonesia, determine the serotype of the local DENV strain through homology modeling, and express the recombinant RdRp protein in Escherichia coli BL21 (DE3), as the target for anti-dengue drug development. …”
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Poly-ADP Ribosyl Polymerase 1 (PARP1) Regulates Influenza A Virus Polymerase by Liset Westera, Alisha M. Jennings, Jad Maamary, Martin Schwemmle, Adolfo García-Sastre, Eric Bortz

“…A number of host factors have been found to regulate the replication of IAV by interacting with the viral RNA-dependent RNA polymerase (RdRP). The host factor PARP1, a poly-ADP ribosyl polymerase, was required for optimal functions of human, swine, and avian influenza RdRP in human 293T cells. …”
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Development and characterization of a reverse genetics system for the lineage II Chicava strain of Machupo virus in a guinea pig model. by Emily Mantlo, Junki Maruyama, John T Manning, Rachel A Reyna, Cheng Huang, Slobodan Paessler

“…<h4>Methods</h4>We report the development of a reverse genetics system for the MACV lineage II Chicava strain, using a pRF42 plasmid encoding the L and S segment genomic RNA under the transcriptional control of a murine DNA-dependent RNA polymerase I promoter sequence. Rescue of the recombinant MACV Chicava strain (rMACV-Chicava) was accomplished by expression of the L protein and nucleoprotein genes of the MACV Carvallo strain in trans in transfected baby hamster kidney (BHK-21) cells. …”
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Four New or Newly Recorded Species from Freshwater Habitats in Jiangxi Province, China by Chen-Yu Xu, Hai-Yan Song, Jian-Ping Zhou, Zhi-Jun Zhai, Chao-Yu Cui, Dian-Ming Hu

“…To study their phylogenetic relationships, the internal transcribed spacer (ITS1-5.8S-ITS2), large subunit (28S, LSU), small subunit (18S, SSU), and RNA polymerase II subunit (<i>RPB2</i>) genes were selected for phylogenetic analyses. …”
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Enitociclib, a selective CDK9 inhibitor: in vitro and in vivo preclinical studies in multiple myeloma by Son Tran, Patrick Sipila, Melanie M. Frigault, Beatrix Stelte-Ludwig, Amy J. Johnson, Joseph Birkett, Raquel Izumi, Ahmed Hamdy, Ranjan Maity, Nizar J. Bahlis, Paola Neri, Aru Narendran

“…Enitociclib inhibited the phosphorylation of the carboxy-terminal domain (CTD) of RNA polymerase II at Ser2/Ser5 and repressed the protein expression of oncogenes c-Myc, myeloid cell leukemia-1 (Mcl-1), and proliferating cell nuclear antigen (PCNA) in MM cells. …”
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PCR Amplification and Transcription for Site-Specific Labeling of Large RNA Molecules by a Two-Unnatural-Base-Pair System by Michiko Kimoto, Rie Yamashige, Shigeyuki Yokoyama, Ichiro Hirao

“…Using these Ds-containing DNA templates and a biotin-linked Pa substrate (Biotin-PaTP) as a modified Pa base, 260-mer RNA transcripts containing Biotin-Pa at a specific position were generated by T7 RNA polymerase. This two-unnatural-base-pair system, combining the Ds-Px and Ds-Pa pairs with modified Pa substrates, provides a powerful tool for the site-specific labeling and modification of desired positions in large RNA molecules.…”
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Update on Remdesivir in the Treatment of Novel Coronavirus Pneumonia by Huang Feng, K. Vijaya Lakshmi, Chin Tat Ng, Murthy Sasikumar, Masita Arip, Masriana Hassan, Voon Kin Chin, Mogana Rajagopal, Omotayo Fatokun, N. Parvathi, Malarvili Selvaraja

“…Remdesivir, a COVID-19 therapeutic medication developed by Gilead Sciences, exerts antiviral effects by suppressing the RNA-dependent RNA polymerase of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). …”
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