Nr5a2 promotes cancer stem cell properties and tumorigenesis in nonsmall cell lung cancer by regulating Nanog by Ting Ye, Jingyuan Li, Zhiwei Sun, Yongli Liu, Liangsheng Kong, Shixia Zhou, Junlin Tang, Jianyu Wang, H. Rosie Xing

“…Using the LLC‐SD model, through transient and stable siRNA interference of Nr5a2 expression, we provided convincing evidence for a regulatory role of Nr5a2 in the maintenance of lung CSC self‐renewal and stem cell properties in vitro. …”
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The imprinted Igf2-Igf2r axis is critical for exosome biogenesis during the early development of bovine placenta by Kunhua ZHENG, Longfei XIAO, Naihan YUAN, Xihui SHENG, Xiaolong QI, Yingqiu WANG, Chang CHEN, Kaijun GUO, Lin YANG, Bingying LIU, Xiangguo WANG

“…Furthermore, the expression of exosomal marker factors was detected after the expression of IGF2R or IGF2 was inhibited through RNA interference or the addition of inhibitors, respectively. …”
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Interleukin 10 Plays an Important Role in Neonatal Rats with Hypoxic-Ischemia Associated with B-Cell Lymphoma 2 and Endoplasmic Reticulum Protein 29 by Xue Bai, Liu-Lin Xiong, Chang-Le Fang, Hao-Li Zhou, Lu-Lu Xue, Yue Hu, Qing-Jie Xia, Jia Liu, Jun-Yan Zhang, Ting-Hua Wang, Si-Jin Yang

“…In addition, Bcl-2 and ERp29 were remarkably decreased following IL-10 mRNA interference compared with the NC group. Our findings revealed that IL-10 exerted its antiapoptotic and neuroprotective effects by regulating the expression of Bcl-2 and ERp29, indicating that IL-10 may be a promising molecule target for HIE treatment.…”
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A ROS-responsive hydrogel encapsulated with matrix metalloproteinase-13 siRNA nanocarriers to attenuate osteoarthritis progression by Qiuyang Wang, Kai Feng, Guangsheng Wan, Wei Liao, Jing Jin, Peng Wang, Xiaolian Sun, Weijun Wang, Qing Jiang

“…Abstract RNA interference (RNAi) and oxidative stress inhibition therapeutic strategies have been extensively utilized in the treatment of osteoarthritis (OA), the most prevalent degenerative joint disease. …”
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Development, opportunities, and challenges of siRNA nucleic acid drugs by Bowen Xiao, Shaopeng Wang, Yu Pan, Wenjun Zhi, Chensheng Gu, Tao Guo, Jiaqi Zhai, Chenxu Li, Yong Q. Chen, Rong Wang

“…The US Food and Drug Administration has approved six siRNA drugs in recent years: patisiran, givosiran, lumasiran, vutrisiran, inclisiran, and nedosiran. siRNA drugs are based on the post-transcriptional gene regulation mechanism of RNA interference. These drugs have gained widespread attention for their effectiveness, low dosage, and low frequency of administration. …”
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PGC-1α Promotes mitochondrial biosynthesis and energy metabolism of goose fatty liver by Jiahui Li, Mengqing Lv, Zijin Yuan, Jing Ge, Tuoyu Geng, Daoqing Gong, Minmeng Zhao

“…The overexpression or RNA interference assay of PGC-1α was performed in goose primary hepatocytes. …”
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Host-Induced Gene Silencing of the <i>Aspergillus flavus O</i>-Methyl Transferase Gene Enhanced Maize Aflatoxin Resistance by Olanike Omolehin, Yenjit Raruang, Dongfang Hu, Zhu-Qiang Han, Surassawadee Promyou, Robert L. Brown, Qijian Wei, Kanniah Rajasekaran, Jeffrey W. Cary, Kan Wang, Dan Jeffers, Zhi-Yuan Chen

“…Maize is one of the major crops that are susceptible to <i>Aspergillus flavus</i> infection and subsequent aflatoxin contamination, which poses a serious health threat to humans and domestic animals. Here, an RNA interference (RNAi) approach called Host-Induced Gene Silencing (HIGS) was employed to suppress the <i>O</i>-methyl transferase gene (<i>omtA</i>, also called <i>aflP</i>), a key gene involved in aflatoxin biosynthesis. …”
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Valerenic acid attenuates pathological myocardial hypertrophy by promoting the utilization of multiple substrates in the mitochondrial energy metabolism by Tiantian Liu, Xu Chen, Qianbin Sun, Junjun Li, Qiyan Wang, Peng Wei, Wei Wang, Chun Li, Yong Wang

“…Methods: The efficacy of VA on PMH was confirmed by in vivo and in vitro experiments and the underlying mechanism was investigated by molecular dynamics (MD) simulations and specific siRNA interference. Results: VA enhanced cardiomyocyte fatty acid oxidation (FAO), inhibited hyper-activated glycolysis, and improved the unbalanced pyruvate-lactate axis. …”
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Neuropilin1-dependent paracrine signaling of cancer cells mediated by miRNA exosomal cargo by Claudia Palazzo, Roberta Mastrantonio, Noemi Gioelli, Erika Testa, Francesco Recco, Donatella Lucchetti, Giulia Villari, Alessio D’Alessio, Alessandro Sgambato, Flavio Mignone, Guido Serini, Maria Teresa Viscomi, Luca Tamagnone

“…Wound healing, transwell, and digital real-time migration assays were carried out to assess the activity of cancer cell-derived exosomes in the regulation of endothelial cells. RNA interference was applied to obtain NRP1 knock-down, and cDNA transfer to achieve its overexpression, in exosome-releasing cells. …”
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ATP1B3 may promote glioma proliferation and migration through MAPK/NF-KB signaling pathway by Qikang Yan, Quan Sun, Yan Feng, Qingyun Hu, Jinling Zhu

“…Its connection to patient survival and prognosis; The siRNA interference approach instantly reduced the amount of ATP1B3 expression in the glioma cell lines U87MG and U251MG. …”
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Identification and expression analysis of the heat shock proteins Hsp70, Hsp90, and Hsp90b in Litopenaeus vannamei under low-temperature stress by Min Peng, Hui Wang, Shuiping Wen, Zheng Liang, Zheng Huang, Bin Zhang, Tianchong Chen, Qingyun Liu, Qiangyong Li, Yuan Meng, Yuliu Huang, Chunling Yang, Digang Zeng, Manyuan Li, Weilin Zhu, Yongzhen Zhao

“…Downstream genes regulated by hsp90, such as akt, were initially upregulated, but then became downregulated, under low-temperature stress. RNA interference-mediated knockdown of hsp90 affected PI3K/AKT pathway signaling, which downregulated the downstream genes akt and apoptosis-inhibiting bcl-xl, bcl-2, and creb, and upregulated the apoptosis-promoting genes casp1 and casp9. …”
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A genomic duplication spanning multiple P450s contributes to insecticide resistance in the dengue mosquito Aedes aegypti by Bacot, Tiphaine, Haberkorn, Chloe, Guilliet, Joseph, Cattel, Julien, Kefi, Mary, Nadalin, Louis, Filee, Jonathan, Boyer, Frederic, Gaude, Thierry, Laporte, Frederic, Tutagata, Jordan, Vontas, John, Dusfour, Isabelle, Bonneville, Jean-Marc, David, Jean-Philippe

“…The involvement of this P450 duplication in deltamethrin survival was supported by a significant phenotypic response to the P450 inhibitor piperonyl butoxide together with genotype-phenotype association and RNA interference. Experimental evolution suggested that this P450 duplication is associated with a significant fitness cost, potentially affecting its adaptive value in presence of other resistance alleles. …”
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甲基转移酶3对人脑血管平滑肌细胞基因表达及增殖和迁移功能的影响 Effects of Methyltransferase 3 on Gene Expression, Proliferation, and Migration Functions of Human Brain Vascular Smooth Muscle Cells... by 孟晨曦1，孙洪英2，张佳2，毛戬2，杨阳3，策乐木格3 (MENG Chenxi1, SUN Hongying2, ZHANG Jia2, MAO Jian2, YANG Yang3, Celemuge3 )

“…Methods The transfected HBVSMC were divided into three groups: the blank group (virus-free), the control group (empty vector), and the interference group (METTL3-shRNA) by using small interfering RNA (shRNA) interference technology and adenovirus packaging method. …”
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