The Long Noncoding RNA LOXL1-AS1 Promotes the Proliferation, Migration, and Invasion in Hepatocellular Carcinoma by Jiang Liu, Chengtong Zhai, Degan Liu, Jianhua Liu

“…MTT assay, colony formation assay, flow cytometry analysis, transwell assays, and lentivirus-mediated RNA interference (RNAi) technology were used to evaluate cell proliferation and migration. …”
Get full text

MAC30 Knockdown Inhibits Proliferation and Enhance Apoptosis of Gastric Cancer by Suppressing Wnt/β-Cateninsignaling Pathway by Xiaohong Wu, Yong Zhang, Junping Guo, Xun Yan, Li Shen, Jianping Zhou, Jun Zhao, Min Zhuang, Zhihong Cao

“…Lentiviral vector pGCSIL-shMAC30-GFP of the RNA interference (RNAi) of the MAC30 gene was transfected into gastric cancer BGC-823 cell line and the expression of lentivirus label protein GFP was observed via fluorescence microscope, while cell proliferation and apoptosis were determined with flow cytometry and MTT assay, respectively. …”
Get full text

LIM-kinase 1 effects on memory abilities and male courtship song in Drosophila depend on the neuronal type by A. V. Zhuravlev, E. S. Zalomaeva, E. S. Egozova, A. D. Emelin, V. V. Sokurova, E. A. Nikitina, E. V. Savvateeva-Popova

“…We applied GAL4 × UAS binary system to induce limk1 RNA interference in different types of neurons. The hybrid strain with limk1 interference in MB lobes and glia showed an increase in 3-h short-term memory (STM), without significant effects on long-term memory. limk1 interference in cholinergic neurons (CHN) impaired STM, while its interference in DAN and serotoninergic neurons (SRN) also dramatically impaired the flies’ learning ability. …”
Get full text

Genetically-encoded targeted protein degradation technology to remove endogenous condensation-prone proteins and improve crop performance by Ming Luo, Sitao Zhu, Hua Dang, Qing Wen, Ruixia Niu, Jiawei Long, Zhao Wang, Yongjia Tong, Yuese Ning, Meng Yuan, Guoyong Xu

“…Abstract Effective modulation of gene expression in plants is achievable through tools like CRISPR and RNA interference, yet methods for directly modifying endogenous proteins remain lacking. …”
Get full text

The biogenesis, regulation and functions of transitive siRNA in plants by Tan Huijun, Liu Yuelin, Guo Hongwei

“…Small RNA (sRNA)-mediated RNA interference (RNAi) is a sequence-specific gene silencing mechanism that modulates gene expression in eukaryotes. …”
Get full text

Potato spindle tuber viroid by A. V. Kochetov, A. Y. Pronozin, N. V. Shatskaya, D. A. Afonnikov, O. S. Afanasenko

“…In the case of PSTVd, disease symptoms may occur due to RNA-interference, which decreases the expression levels of some important cellular regulatory factors, such as, for example, potato StTCP23 from the gibberellic acid pathway with a role in tuber morphogenesis or tomato FRIGIDA-like protein 3 with an early flowering phenotype. …”
Get full text

Phytoalexin deficient 4 is associated with the lesion mimic trait in watermelon clalm mutant (Citrullus lanatus) by Jiale Shi, Congji Yang, Yuanyuan Qin, Qingqing Liu, Shengqi Hua, Defeng Wu, Wei Dong

“…Overexpression the ClCG04G001930 gene, designated as ClPAD4, yielded transgenic lines whose leaves gradually developed chlorotic lesions over 3 weeks. RNA interference of the ClPAD4 yielded transgenic lines whose cotyledon prone to diseased over 2 weeks. …”
Get full text

Downregulation of VRK1 Inhibits Progression of Lung Squamous Cell Carcinoma through DNA Damage by Ning Du, Boxiang Zhang, Yunfeng Zhang

“…VRK1 shRNA was transfected into LUSC NCI-H520 and SK-MES-1 cell lines to interfere with VRK1 expression, and the efficiency of VRK1 shRNA interference was detected by the western blot. The effects of VRK1 downregulation on LUSC cell viability, migration, cell cycle, and apoptosis were analyzed by the CCK8 assay, scratch assay, transwell assay, and flow cytometry. …”
Get full text

Decreased SynMuv B gene activity in response to viral infection leads to activation of the antiviral RNAi pathway in C. elegans. by Ashwin Seetharaman, Himani Galagali, Elizabeth Linarte, Mona H X Liu, Jennifer D Cohen, Kashish Chetal, Ruslan Sadreyev, Alex J Tate, Taiowa A Montgomery, Gary Ruvkun

“…RNA interference (RNAi) mediates antiviral defense in many eukaryotes. …”
Get full text

Spray-Induced Gene Silencing (SIGS): Nanocarrier-Mediated dsRNA Delivery Improves RNAi Efficiency in the Management of Lettuce Gray Mold Caused by <i>Botrytis cinerea</i> by Maria Spada, Claudio Pugliesi, Marco Fambrini, Diego Palpacelli, Andrea Caneo, Susanna Pecchia

“…In this scenario, the strategy of RNA interference (RNAi) is being widely considered, and Spray-Induced Gene Silencing (SIGS) is gaining interest as a versatile, sustainable, effective, and environmentally friendly alternative to the use of chemicals in the protection of crops. …”
Get full text

Migration of primordial germline cells is negatively regulated by surrounding somatic cells during early embryogenesis in Drosophila melanogaster by N. V. Dorogova, A. S. Khruscheva, Iu. A. Galimova, D. Yu. Oshchepkov, D. E. Maslov, E. D. Shvedkina, K. A. Akhmetova, S. A. Fedorova

“…We identified the myc, Hph, stat92E, Tre-1, and hop genes, whose RNA interference leads to premature active PGC migration at stages 4–7 of embryogenesis. …”
Get full text

The transcription factor HMGB2 indirectly regulates APRIL expression and Gd-IgA1 production in patients with IgA nephropathy by Huijuan Tian, Yaling Zhai, Shuaigang Sun, Wenhui Zhang, Zhanzheng Zhao

“…In addition, we used DNA pull-down, protein profiling, and transcription factor prediction software to identify proteins bound to the promoter region of the APRIL gene. RNA interference and coimmunoprecipitation (Co-IP) were used to verify the relationships among HMGB2, high mobility group AT-hook protein 1 (HMGA1), and APRIL.Results HMGB2 expression was greater in IgAN patients than in HCs and was positively associated with APRIL expression in B cells. …”
Get full text

Glutamine-glutamate centered metabolism as the potential therapeutic target against Japanese encephalitis virus-induced encephalitis by Mengyuan Li, Hang Yuan, Xiaofei Yang, Yingfeng Lei, Jianqi Lian

“…Exogenous addition of metabolites and associated compounds and RNA interference were employed to manipulate glutamine-glutamate metabolism to clarify its effects on viral replication. …”
Get full text

Long non-coding RNA MIR4435-2HG modulates pancreatic cancer stem cells and chemosensitivity to gemcitabine by targeting the miR-1252-5p/STAT1 by Baocheng Xie, Peishan Wu, Hongyu Liu, XiangDi Yang, Linxuan Huang

“…Functional assays were conducted to evaluate CSC self-renewal, tumorigenic potential, and chemoresistance in pancreatic cancer cells with altered expression of MIR4435-2HG. RNA interference (RNAi) was employed to knock down MIR4435-2HG, and a STAT1 reintroduction model was established to examine downstream signaling pathways. …”
Get full text

Protective effect of brain and muscle arnt-like protein-1 against ethanol-induced ferroptosis by activating Nrf2 in mice liver and HepG2 cells by Yanan Zhao, Ranran Zhang, Ziheng Chen, Ziyi Wang, Shuang Guan, Jing Lu

“…Further study indicated that the alcohol could activate the protein expression of brain and muscle arnt-like protein-1 (BMAL1) which exerts a protective effect against ferroptosis through promoting nuclear factor erythroid 2-related factor 2 (Nrf2) translocation into nuclear and subsequently stimulating its downstream proteins FTH, FPN, glutathione peroxidase 4 activity (GPX4), HO-1, SLC7A11, while knockdown of BMAL1 and Nrf2 by RNA interference further downregulated the expression of these protein and thus promoting ferroptosis in response to alcohol. …”
Get full text

Microbiota governs host chenodeoxycholic acid glucuronidation to ameliorate bile acid disorder induced diarrhea by Zishen Lin, Yue Feng, Jinping Wang, Zhaoyue Men, Xi Ma

“…In vitro, important gene involved in the reduction of BA disorder induced-diarrhea were screened by RNA transcriptomics sequencing, and activation pathway of FXR-SIRT1-LKB1 to alleviate BA disorder diarrhea and P53-mediated apoptosis were proposed in vitro by multifarious siRNA interference, CO-IP, immunofluorescence, and so on, which mechanism was also verified in a variety of mouse models. …”
Get full text

Co-modulation of a circular form of PCDH11Y during neuroendocrine differentiation of prostate cancer by Giovanni Pecoraro, Ilaria Leone, Silvia Nuzzo, Santiago Negueruela, Giovanni Smaldone, Lorena Buono

“…After that, validation and functional analysis by RNA-interference was applied to a selected circRNA to explore its role during NED. …”
Get full text

Selective suppression of influenza A/H5N1 virus replication <i>in vitro</i> using nanocomplexes consisting of siRNA and aminopropylsilanol nanoparticles by Marina N. Repkova, Asya S. Levina, Oleg Yu. Mazurkov, Elena V. Makarevich, Ekaterina I. Filippova, Natalya A. Mazurkova, Valentina F. Zarytova

“…It has been shown that the double-stranded siRNA molecules in the nanocomplexes, which act by the RNA interference mechanism, are more efficient in inhibiting the replication of the influenza virus than the corresponding single-stranded RNA fragments. …”
Get full text

SHP2 promotes the epithelial-mesenchymal transition in triple negative breast cancer cells by regulating β-catenin by Shihan Qian, Jingjing Zhu, Qing Han, Huang Cheng, Huaibin Zhou

“…Furthermore, we knocked down SHP2 expression via RNA interference in three TNBC cell lines. To assess the impact of SHP2 on invasion and migration, we conducted transwell assays and wound healing experiments. …”
Get full text

AmelOBP4: an antenna-specific odor-binding protein gene required for olfactory behavior in the honey bee (Apis mellifera) by Fang Liu, Yu Lai, Lixian Wu, Qiang Li, Linyue Lei, Wei Yin, Yuan Zhang, Zachary Y. Huang, Hongxia Zhao

“…Previously, we found that AmelOBP4 has a significantly higher expression in the heads of foragers than that of nurses regardless of their ages, revealing its importance in foraging behaviour of the honey bee. RNA interference (RNAi) is the induction of sequence specific gene silencing by double-stranded RNA (dsRNA), it is a powerful tool that makes gene inactivation possible in organisms that were not amenable to genetic analysis before. …”
Get full text