The m6A modification regulates the composition of myofiber types in chicken skeletal muscle by Yanju Shan, Yifan Liu, Ming Zhang, Lichuan Pang, Gaige Ji, Xiaojun Ju, Yunjie Tu, Jingting Shu

“…As a widespread epigenetic RNA modification, N6-methyladenosine (m6A) plays essential regulatory roles in multiple biological processes. …”
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Artificially Cultivated Ophiocordyceps sinensis Alleviates Diabetic Nephropathy and Its Podocyte Injury via Inhibiting P2X7R Expression and NLRP3 Inflammasome Activation by Chao Wang, Xiao-xia Hou, Hong-liang Rui, Li-jing Li, Jing Zhao, Min Yang, Li-jun Sun, Hong-rui Dong, Hong Cheng, Yi-Pu Chen

“…The experiments in vivo and in vitro both displayed that the mRNA and protein expression of P2X7R, NLRP3, ASC, caspase1 (procaspase-1 mRNA in the gene level and active caspase-1 subunit P10 in the protein level), IL-1β, and IL-18 was significantly upregulated and the mRNA and protein expression of podocyte-associated molecules was significantly changed (downregulation of nephrin, podocin, and WT-1 expression and upregulation of desmin expression) indicating podocyte injury in the kidney tissue of DN rats and in the HG-stressed mouse podocytes, respectively. …”
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FORCE platform overcomes barriers of oligonucleotide delivery to muscle and corrects myotonic dystrophy features in preclinical models by Timothy Weeden, Tyler Picariello, Brendan Quinn, Sean Spring, Pei-Yi Shen, Qifeng Qiu, Benjamin F. Vieira, Lydia Schlaefke, Ryan J. Russo, Ya-An Chang, Jin Cui, Monica Yao, Aiyun Wen, Nelson Hsia, Tama Evron, Katy Ovington, Pei-Ni Tsai, Nicholas Yoder, Bo Lan, Reshmii Venkatesan, John Hall, Cody A. Desjardins, Mo Qatanani, Scott Hilderbrand, John Najim, Zhenzhi Tang, Matthew K. Tanner, Romesh Subramanian, Charles A. Thornton, Oxana Ibraghimov-Beskrovnaya, Stefano Zanotti

“…In patient-derived myoblasts, DYNE-101 reduces DMPK RNA and nuclear foci, consequently improving spliceopathy. …”
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CircHipk3 serves a dual role in macrophage pyroptosis by promoting NLRP3 transcription and inhibition of autophagy to induce abdominal aortic aneurysm formation by Donghua Cai, Chuling Li, Yingyuan Zhang, Sisi He, Yihai Guo, Wangjun Liao, Yulin Liao, Jianping Bin, Xiang He

“…Mechanically, chromatin isolation by RNA purification (ChIRP) indicated that circHipk3 facilitated macrophage pyroptosis by interaction with Stat3, increase the NLRP3 level in the aorta, and by binding Snd1 to promote Ptbp1 mRNA degradation to inhibit autophagy. …”
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CircPRMT5, a Potential Salivary Biomarker, Facilitates the Progression of Head and Neck Squamous Cell Carcinoma via the IGF2BP3-SERPINE1 Pathway by Jiang S, Ou L, Wang Y, Su K, Chen Z, He L, Xu X, Cheng B, Xia J, Fan Z

“…Mechanistically, circPRMT5 was demonstrated to directly bind to and stabilize the insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3), which, subsequently, binds to and stabilizes the serpin family E member 1 (SERPINE1) mRNA, thereby enhancing SERPINE1 expression. …”
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Binding of Dumbbell Oligonucleotides to MoMuLV Reverse Transcriptase: Inhibitory Properties of RNase H Activity by Ajay Kumar

“…The inhibitory properties of the retroviral RNase H activity was evaluated using 3H –UTP-labeled RNA:RNA-DNA hybrid. It was found that the best dumbbell oligonucleotide, inhibitor contained phosphorothioate residues in both the loops. …”
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Linear Methods for Analysis and Quality Control of Relative Expression Ratios from Quantitative Real-Time Polymerase Chain Reaction Experiments by Robert B. Page, Arnold J. Stromberg

“…One of the most frequently used expression measures that results from such experiments is the relative expression ratio (RE), which describes expression in experimental samples (i.e., RNA isolated from organisms, tissues, and/or cells that were exposed to one or more experimental or nonbaseline condition) in terms of fold change relative to calibrator samples (i.e., RNA isolated from organisms, tissues, and/or cells that were exposed to a control or baseline condition). …”
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Transcriptome Sequencing and Bioinformatics Analysis of Ovarian Tissues from Pomacea canaliculata in Guangdong and Hunan by Jing Liu, Jian Li, Zhi Wang, Hua Yang

“…Through high-throughput RNA sequencing (RNA-seq), we analyzed the ovarian tissue of the snails in Guangdong (G_O) and those in Hunan (H_O) using comparative analysis of transcription. …”
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Assessment for Melting Temperature Measurement of Nucleic Acid by HRM by Jing Wang, Xiaoming Pan, Xingguo Liang

“…Finally, HRM method was also successfully used to measure Tms of DNA triplex, hairpin, and RNA duplex. In conclusion, HRM can be applied in the evaluation of thermal stability of nucleic acid (DNA or RNA) or secondary structural elements (even when dNTPs are present).…”
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First record of a cold-seep squat lobster Munidopsis lauensis Baba and de Saint Laurent, 1992 (Anomura: Galatheoidea) from Indian waters by Maria-Judith Gonsalves, Shivam Tiwari, Vinay P. Padate, V. Deepak Samuel, Jigneshkumar Trivedi

“…Molecular analysis of the partial mitochondrial gene coding for cytochrome C oxidase subunit I (COI mtDNA), and mitochondrial 16S ribosomal RNA (16S rRNA) validated its identity. The present observation extends its geographical distribution to the Northern Indian Ocean. …”
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The Roles of circRNAs in Intervertebral Disc Degeneration: Inflammation, Extracellular Matrix Metabolism, and Apoptosis by Hao Li, Lijun Tian, Jianhua Li, Yongjin Li, Lilong Du, Zhenxin Huo, Baoshan Xu

“…Circular RNAs (circRNAs), as a kind of noncoding RNA (ncRNA), participate in the regulation of genetic transcription and translation and further affect the expression of inflammatory cytokine, metabolism of extracellular matrix (ECM), the proliferation and apoptosis of cells, etc. …”
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Targeting Toll-Like Receptors for Treatment of SLE by Christopher G. Horton, Zi-jian Pan, A. Darise Farris

“…Among these, the endosomal TLRs 3, 7/8, and 9 recognize dsRNA, ssRNA, and CpG DNA, respectively. Nucleic acid-sensing TLRs, TLR 7 in particular, have been implicated in systemic lupus erythematosus (SLE) and are thought to exacerbate disease pathology. …”
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Complete mitochondrial genome and phylogenetic analysis of the Atrato slider, Trachemys medemi (Testudines, Emydidae) by Sebastián Cuadrado-Ríos, Mario Vargas-Ramírez, Christian Kehlmaier, Uwe Fritz

“…It includes 13 protein-coding genes, two rRNA genes, 22 tRNA genes, and the non-coding control region. …”
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Application of Spatial Transcriptomics in Digestive System Tumors by Bowen Huang, Yingjia Chen, Shuqiang Yuan

“…This review also discusses the importance of combining spatial transcriptomics with single-cell RNA sequencing (scRNA-seq), artificial intelligence, and machine learning in digestive system cancer research.…”
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Monoclonal Gammopathy of Undetermined Significance (MGUS) in a Man with Fragile X-associated Tremor/Ataxia Syndrome by Tanjung A. Sumekar, Aneel A. Ashrani, Tri I. Winarni, Randi J. Hagerman

“…Both MGUS and FXTAS are associated with microRNA (miRNA) dysregulation. We speculate that individuals with FXTAS may be predisposed to MGUS and further studies are warranted regarding this association.…”
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Effects of elongation delay in transcription dynamics by Xuan Zhang, Huiqin Jin, Zhuoqin Yang, Jinzhi Lei

“…In the transcription process, elongation delay is induced by the movement of RNA polymerases (RNAP) along the DNA sequence, and can result in changes in the transcription dynamics. …”
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COVID-19 Booster Vaccines Administration in Different Countries by Angel Yun-Kuan Thye, Loh Teng-Hern Tan, Jodi Woan Fei Law, Vengadesh Letchumanan

“…The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that resulted in the COVID-19 global pandemic had consequently led to the development of different types of COVID-19 vaccines, including the messenger RNA (mRNA) vaccines, inactivated virus vaccines, a protein subunit vaccine, and viral vector recombinant vaccines. …”
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Two complete chloroplast genomes of Ceratophyllum, an aquatic genus with unresolved phylogenetic position by Shuangyan Ru, Zhigang Wu, Huijun Wang, Qi Li, Tao Li

“…The chloroplast genomes of Ceratophyllum encode 113 unique genes, including 79 protein-coding genes, four rRNA genes, and 30 tRNA genes. The assembly of these two chloroplast genomes not only contributes to our understanding of the genome of Ceratophyllum but also provides valuable insights for the evolutionary study of angiosperms.…”
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Particulate Matter-Induced Acute Coronary Syndrome: MicroRNAs as Microregulators for Inflammatory Factors by Nur Izah Ab Razak, Nor Eliani Ezani, Norzian Ismail

“…Endogenous miRNAs, average 22-nucleotide RNAs (ribonucleic acid), regulate gene expression through mRNA cleavage or translation repression and can influence proinflammatory gene expression posttranscriptionally. …”
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Integration of unpaired single cell omics data by deep transfer graph convolutional network. by Yulong Kan, Yunjing Qi, Zhongxiao Zhang, Xikeng Liang, Weihao Wang, Shuilin Jin

“…The rapid advance of large-scale atlas-level single cell RNA sequences and single-cell chromatin accessibility data provide extraordinary avenues to broad and deep insight into complex biological mechanism. …”
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