An Independent Prognostic Model Based on Ten Autophagy-Related Long Noncoding RNAs in Pancreatic Cancer Patients by Jiahui Tian, Chunyan Fu, Xuan Zeng, Xiaoxiao Fan, Yi Wu

“…This study aims to explore the feasibility of using an autophagy-related long noncoding RNA (lncRNA) signature for assessing PC patient survival. …”
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In Silico Analysis of Common Long Noncoding RNAs in Schistosoma mansoni and Schistosoma haematobium by Serhat Sirekbasan, Tugba Gurkok Tan

“…In S. mansoni and S. haematobium, 5132 and 3589 lncRNA transcripts were detected, respectively. These two species had 694 lncRNAs in common. …”
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Implications of the Use of Eukaryotic Translation Initiation Factor 5A (eIF5A) for Prognosis and Treatment of Hepatocellular Carcinoma by Felix H. Shek, Sarwat Fatima, Nikki P. Lee

“…In eukaryotes, translation of messenger RNA (mRNA) to protein is a key process in protein biosynthesis in which initiation of translation involves interaction of different eukaryotic translation initiation factors (eIFs), ribosome subunits and mRNAs. …”
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How energy determines spatial localisation and copy number of molecules in neurons by Cornelius Bergmann, Kanaan Mousaei, Silvio O. Rizzoli, Tatjana Tchumatchenko

“…We found that energy minimisation principles explain experimentally-reported exponential rank distributions of mRNA and protein copy numbers. Our results further reveal robust energy benefits when certain mRNA classes are moved into dendrites, for example mRNAs of proteins with long amino acid chains or mRNAs with large non-coding regions and long half-lives proving surprising insights at the level of molecular populations.…”
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Ovarian Development and Vitellogenin Gene Expression under Heat Stress in Silkworm, Bombyx mori by Satinath Paul, Bela Keshan

“…The study further showed a developmental- and tissue-specific expression of vitellogenin mRNA in fat body and ovary upon heat shock. When heat shock treatment was done at 39°C and 42°C to spinning larvae, ovary showed an upregulation in the expression of vitellogenin mRNA, whereas fat body failed to do so. …”
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AGD1/USP10/METTL13 complexes enhance cancer stem cells proliferation and diminish the therapeutic effect of docetaxel via CD44 m6A modification in castration resistant prostate can... by Hong Wang, Chunli Cui, Weiyi Li, Hui Wu, Jianjun Sha, Jiahua Pan, Wei Xue

“…To uncover the underlying mechanisms of AGD1, RNA pull-down assay, RIP, co-Immunoprecipitation (co-IP), mass spectrometry (MS), Methylated RNA immunoprecipitation (MeRIP) and single-base elongation and ligation-based qPCR amplification method (SELECT) were performed. …”
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Silencing hepatic PCSK9 via novel chimeric AAV8 mitigates the progression of atherosclerosis by inhibiting inflammation in ApoE−/− mice by Xiaocui Chen, Minghui Sun, Xiang Ma, Yitong Ma, Bangdang Chen

“…Here, we developed a novel chimeric AAV8.P-PCSK9 short hairpin RNA (shRNA) vector that incorporates a cross-species specific shRNA targeting PCSK9 to assess its effects on lipid levels and atherosclerosis in mice. …”
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Overexpression of MMP13 Is Associated with Clinical Outcomes and Poor Prognosis in Oral Squamous Cell Carcinoma by Vui King Vincent-Chong, Iman Salahshourifar, Lee Peng Karen-Ng, Ming Yhong Siow, Thomas George Kallarakkal, Anand Ramanathan, Yi-Hsin Yang, Goot Heah Khor, Zainal Ariff Abdul Rahman, Siti Mazlipah Ismail, Narayanan Prepageran, Wan Mahadzir Wan Mustafa, Mannil Thomas Abraham, Keng Kiong Tay, Sok Ching Cheong, Rosnah Binti Zain

“…Despite discrepancies between gene amplification and mRNA and protein overexpression rates, OSCC cases showed high amplification of MMP13 and overexpression of MMP13 at both mRNA and protein levels. …”
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Upregulated SAE1 Drives Tumorigenesis and Is Associated with Poor Clinical Outcomes in Breast Cancer by Hong Liu, Jing Wang, Yunhai Li, Feng Luo, Lei Xing

“…Cell cycle progression was analyzed by flow cytometry, and the expression of cell cycle-related proteins (E2F1, cyclin D3, and cyclin-dependent kinase 2) was determined by western blots in SAE1 small interfering RNA (siRNA) transfected cells. The GSE1456 dataset was used to analyze possible signal pathways associated with SAE1 by gene set enrichment analysis (GSEA), and the expression of PI3K/AKT/mTOR pathway-related proteins (such as p-PI3K, p-AKT, and mTOR) in SAE1-siRNA cells was detected by western blots. …”
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Homortomyces gen. nov., a new dothidealean pycnidial fungus from the Cradle of Humankind by Michael J. Wingfield, Lorenzo Lombard, Johannes Z. Groenewald, Pedro W. Crous

“…Its morphology and phylogenetic placement based on analyses of sequence data for the large subunit nuclear ribosomal RNA gene (LSU, 28S) as well as the ITS and 5.8S rRNA gene of the nrDNA operon, show that Homortomyces represents a novel genus in Dothideomycetes, although its familial relationships remain unresolved.…”
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Targeting NANOS1 in triple-negative breast cancer: synergistic effects of digoxin and PD-1 inhibitors in modulating the tumor immune microenvironment by Tangyi Wang, Yadian Lei, Jingwei Sun, Li Wang, Yuxin Lin, Zhijing Wu, Shoude Zhang, Chengzhu Cao, Chengzhu Cao, Chengzhu Cao, Haiyan Wang, Haiyan Wang, Haiyan Wang

“…Flow cytometry was applied to assess lymphocyte infiltration in the tumor immune microenvironment. RNA-seq analysis after target silencing by small interfering RNA (siRNA) was performed, followed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. …”
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Creation of body color mutants by CRISPR/Cas9 gene editing in largemouth bass, Micropterus salmoides by Lina Sun, Tian Gao, Zhiqiang Li, Xiaolong Yang, Zuliang Qin, Mingxiao Ye, Ying Li, Feilong Wang, Deshou Wang

“…The efficiency of mutation was gRNA and Cas9 mRNA concentration dependent, and 400 ng/μl gRNA and 800 ng/μl Cas9 mRNA were recommended as the appropriate injection dose for genome editing in LMB. …”
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Comprehensive Analysis of Differentially Expressed miRNAs and mRNAs Reveals That miR-181a-5p Plays a Key Role in Diabetic Dermal Fibroblasts by Peng Liu, Yi Zhu, Qin Li, Biao Cheng

“…We searched the GEO database for related miRNA (GSE68185, GSE84971) and mRNA (GSE49566, GSE78891) profiles. …”
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Impact of Exercise on Tramadol-Conditioned Place Preference by Haneen Amawi, Alaa M. Hammad, Aseel Abdullah Ibrahim, Nosyba Alsbih, Frank Scott Hall, Fawaz Alasmari, Bahaa Al-Trad

“…Additionally, the TRA-induced elevations in <i>Il-1β</i> mRNA expression were reversed by all durations of exercise in Hipp. …”
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Identification of a New m6A Regulator-Related Methylation Signature for Predicting the Prognosis and Immune Microenvironment of Patients with Pancreatic Cancer by Tianle Zou, Dan Shi, Weiwei Wang, Guoyong Chen, Xianbin Zhang, Yu Tian, Peng Gong

“…N6-methyladenosine (m6A) is involved in a wide variety of biological activities due to the fact that it is the most common form of mRNA modification in mammals. Numerous research has accumulated evidence suggesting that a malfunction in the regulation of m6A RNA modification is associated with various illnesses, including cancers. …”
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Effects of neonatal handling on behavior and stress-response in rats selected for reaction towards humans by Yu. E. Herbeck, O. A. Amelkina, M. Yu. Konoshenko, S. G. Shikhevich, R. G. Gulevich, R. V. Kozhemyakina, I. Z. Plyusnina, I. N. Oskina

“…Neonatal handling decreased the amount of mRNA for the glucocorticoid receptor (GR) in the hippocampus of aggressive rats but did not affect significantly the amount of mRNA for the corticotropin-releasing hormone (CRH) in the hypothalamus. …”
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METTL3-dependent DLG2 inhibits the malignant progression of cervical cancer by inactivating the Hippo/YAP signaling by Mei Pu, Xia Xiao, Shasha Lv, Daqing Ran, Qian Huang, Mingming Zhou, Qirong Lei, Lingshuang Kong, Qing Zhang

“…The METTL3-dependent regulation of DLG2 mRNA stability could be a critical factor in cervical cancer progression.…”
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Differential expressed genes (DEGs) and differential alternative splicing genes (DASs) revealed the common pathologic mechanism of three bacterial pathogens to American eels (Angui... by Zihao Chen, Guanghua Sun, Songlin Guo

“…Subsequently, the pathological changes and RNA-seq analysis of the spleen were explored after 36 and 60 hours (h) of the infection. …”
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Knockdown of hexokinase 2 results in a decreased expression level of the glycolytic enzymes PFKP, BPGM, and GPI in RKO cell line by M. S. Fedorova, I. Y. Karpova, A. V. Lipatova, E. A. Pudova, Z. G. Guvatova, D. V. Kochetkov, A. V. Chaika, B. Y. Alekseev, M. V. Kiseleva, A. D. Kaprin, A. V. Kudryavtseva, A. V.  Snezhkina

“…Quantitative expression analysis of 15 genes (GAPDH, ADPGK, ALDOA, ENO3, PFKL, PGK1, PGAM1, PKM2, ENO1, PDK1, PDK3, PFKP, ENO2, GPI, and BPGM), encoding the key glycolysis enzymes, as well as HIF1A gene was carried out in a modified RKO cell line, which constantly expresses the short hairpin RNA (shRNA) for the inhibition of hexokinase 2. …”
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Identification and functional analysis of energy metabolism and pyroptosis-related genes in diabetic nephropathy by Shan He, Jian Ye, Yu Wang, Lu yang Xie, Si Yi Liu, Qin kai Chen

“…Subsequent analyses included Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment, Gene Set Enrichment Analysis (GSEA), and Protein-Protein Interaction (PPI) network analysis. Additionally, mRNA-miRNA, mRNA-drug, and mRNA-transcription factor (TF) interaction networks were constructed. …”
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