Transient ocular hypertension remodels astrocytes through S100B. by Weiran Huang, Kenji Matsushita, Rumi Kawashima, Susumu Hara, Yuichi Yasukura, Kaito Yamaguchi, Shinichi Usui, Koichi Baba, Andrew J Quantock, Kohji Nishida

“…Glial fibrillary acidic protein (GFAP), S100B, and actin staining were used to characterize astrocyte morphology and cytoskeleton, with qPCR used to measure mRNA expression. We also silenced S100B expression and conduct RNA sequencing on ONH astrocytes. …”
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Liver Microbiome in Healthy Rats: The Hidden Inhabitants of Hepatocytes by Xiao Wei Sun, Hua Zhang, Xiao Zhang, Peng Fei Xin, Xue Gao, Hong Rui Li, Cai Yun Zhou, Wen Min Gao, Xuan Xuan Kou, Jian Gang Zhang

“…The V3–V4 region of the 16S rRNA gene was amplified by polymerase chain reaction (PCR) and sequenced using an Illumina HiSeq 2500 platform. …”
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CpG Oligodeoxynucleotides Inhibit RANKL-Induced Osteoclast Formation by Upregulating A20 Deubiquitinase in RAW 264.7 Cells by Seong-Kyu Kim, Jung-Yoon Choe, Ki-Yeun Park

“…Number of TRAP-positive cells transfected with A20 siRNA was higher than those transfected with NC siRNA. …”
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Influence of Fasting Plasma Glucose Level on Admission of COVID-19 Patients: A Retrospective Study by Yingying Zhao, Huichun Xing

“…Furthermore, the risk factors for prolonged duration of SARS-CoV-2 RNA shedding were identified, and the relationship between FPG levels and the prolonged presence of SARS-CoV-2 RNA was evaluated. …”
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Screening of necroptosis-related genes and evaluating the prognostic capacity, clinical value, and the effect of their copy number variations in acute myeloid leukemia by Dake Wen, Ru Yan, Lin Zhang, Haoyang Zhang, Xuyang Chen, Jian Zhou

“…The expression levels of the hub-NRDEGs were validated in vitro. The mRNA-miRNA and mRNA-TF interaction networks with the hub-NRDEGs were screened using Cytoscape@. …”
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Liver Expression of Sulphotransferase 2A1 Enzyme Is Impaired in Patients with Primary Sclerosing Cholangitis: Lack of the Response to Enhanced Expression of PXR by Ewa Wunsch, Marta Klak, Urszula Wasik, Malgorzata Milkiewicz, Malgorzata Blatkiewicz, Elzbieta Urasinska, Olivier Barbier, Dariusz Bielicki, Dimitrios P. Bogdanos, Elwyn Elias, Piotr Milkiewicz

“…Genomic DNA was isolated from blood of PSC patients (n=120) and an equal number of healthy volunteers. Liver miRNA expression was evaluated using Affymetrix-Gene-Chip miRNA4.0. …”
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Biphasic Modulation of NOS Expression, Protein and Nitrite Products by Hydroxocobalamin Underlies Its Protective Effect in Endotoxemic Shock: Downstream Regulation of COX-2, IL-1β,... by André L. F. Sampaio, Jesmond Dalli, Vincenzo Brancaleone, Fulvio D'Acquisto, Mauro Perretti, Carmen Wheatley

“…During the immune response, pro-inflammatory phase, parenteral hydroxocobalamin (HOCbl) treatment partially inhibits hepatic, but not lung, iNOS mRNA and promotes lung eNOS mRNA, but attenuates the LPS hepatic rise in eNOS mRNA, whilst paradoxically promoting high iNOS/eNOS protein translation, but relatively moderate •NO production. …”
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First report of the chloroplast and mitochondrial genomes of the Indian pitcher plant, Nepenthes khasiana Hook.f. by Ruchishree Konhar, Debasis Dash, Devendra Kumar Biswal

“…The cp genome contains two repeat regions and 131 genes (112 unique genes): 86 protein coding genes, 8 rRNA coding genes and 37 tRNA coding genes. The mt genome contains 84 genes (55 unique genes): 50 protein coding genes, 7 rRNA coding genes and 27 tRNA coding genes. …”
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Identification of Type VI Collagen Synthesizing Cells in Human Diabetic Glomerulosclerosis Using Renal Biopsy Sections by Mohammed Shawkat Razzaque, Takehiko Koji, Takashi Harada, Takashi Taguchi

“…For cellular localization of type VI collagen mRNA, paraffin‐embedded renal sections of the control and DN were hybridized in situ with digoxigenin (Dig)‐labeled antisense oligo‐DNA probe complementary to a part of \alpha1 (VI) mRNA. …”
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Whole-Genome Sequence of Streptomyces pluripotens strain MUM 16J, a Potential Resource of Glycopeptide Antibiotic and Biocontrol Agent against Biofilm-forming Bacteria by Priyia Pusparajah, Jodi Woan-Fei Law, Kok-Gan Chan, Bey-Hing Goh, Kar-Wai Hong, Learn-Han Lee, Loh Teng-Hern Tan

“…Its genome consists of 6,781 predicted genes, 6,459 protein-coding genes, and 79 RNA-coding genes (tRNA: 71, rRNA: 8). Here we report the draft genome of this strain to highlight its potential in producing glycopeptide antibiotic as well as catechol, a biocontrol agent of biofilm-producing bacteria. …”
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Circulating MicroRNAs in Plasma of Hepatitis B e Antigen Positive Children Reveal Liver-Specific Target Genes by Thilde Nordmann Winther, Kari Stougaard Jacobsen, Aashiq Hussain Mirza, Ida Louise Heiberg, Claus Heiner Bang-Berthelsen, Flemming Pociot, Birthe Hogh

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Whole-Genome Sequence of Chelatococcus daeguensis Strain M38T9, Isolated from Ulu Slim Hot Spring in Malaysia by Yi Xian Goh, Kok Gan Chan, Kar Wai Hong

“…There are 4,046 predicted genes, 3,962 protein-coding genes, and 53 RNA-coding genes (tRNA: 45, rRNA: 4). The draft genome has been deposited at DDBJ/ENA/GenBank under the BioProject accession number PRJNA668056. …”
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The Transcribed-Ultraconserved Regions: A Novel Class of Long Noncoding RNAs Involved in Cancer Susceptibility by Paola Scaruffi

“…Focus on such sequences has revealed numerous RNA subtypes with several functions in RNA processing and gene expression regulation, and deep sequencing studies imply that many remain to be discovered. …”
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Clinicopathologic Significance of CXCL12 and CXCR4 Expressions in Patients with Colorectal Cancer by Naomi Yoshuantari, Didik Setyo Heriyanto, Susanna Hilda Hutajulu, Johan Kurnianda, Ahmad Ghozali

“…The quantitative expressions of CXCL12 and CXCR4 messenger RNA (mRNA) were evaluated in 32 patients with adenocarcinoma-type CRC. …”
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The Correlation of PPARα Activity and Cardiomyocyte Metabolism and Structure in Idiopathic Dilated Cardiomyopathy during Heart Failure Progression by E. Czarnowska, D. Domal-Kwiatkowska, E. Reichman-Warmusz, J. B. Bierla, A. Sowinska, A. Ratajska, K. Goral-Radziszewska, R. Wojnicz

“…There were analogous changes in the expression of FAT/CD36 and CPT-1 mRNA in contrast to continuous overexpression of GLUT-4 mRNA and significant increase of PDK-4 mRNA in Group (II). …”
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Molecular Detection of Anaplasma phagocytophilum in Small Mammals and Infesting Ticks in Laikipia County, Kenya by Erick Titus Mosha, Joseph K. N. Kuria, Moses Otiende, Isaac Lekolool

“…The blood samples were subjected to a nested PCR-HRM melt analysis using species-specific primers to amplify the 16S ribosomal RNA genes. The ticks were also subjected to nested PCR-HRM involving 16S rRNA gene primers. …”
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M6a demethylase FTO regulates the oxidative stress, mitochondrial biogenesis of cardiomyocytes and PGC-1a stability in myocardial ischemia-reperfusion injury by Qiong Jiang, Xuehai Chen, Kezeng Gong, Zhe Xu, Lianglong Chen, Feilong Zhang

“…The m6A dot blot, methylated RNA immunoprecipitation PCR (MeRIP-PCR) and RNA stability analysis were used to analyze the regulation of methylation of PGC-1a by FTO.Results FTO was downregulated in MIRI rats and H/R induced cardiomyocytes. …”
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Exploring the prognostic role of microbial and genetic markers in lung squamous cell carcinoma by Fan Yang, Xiaodong Jia, Zihuan Ma, Siyao Liu, Chunzi Liu, Dan Chen, Xiuju Wang, Niansong Qian, Hui Ma

“…LUSC patient data from The Cancer Genome Atlas (TCGA), including microbial genus level abundance data and RNA sequencing (RNA-Seq) data, were used as a training dataset. …”
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Alterations of Dopamine-Related Transcripts in A11 Diencephalospinal Pathways after Spinal Cord Injury by Shunyi Zhao, Jaclyn H. DeFinis, Shaoping Hou

“…After 3 or 8 weeks, rostral (T5) and caudal (L1) spinal cord tissue was collected to measure mRNA levels of DA-related genes. Meanwhile, A11 neurons in the brain were explicitly isolated by laser capture microdissection, and single-cell qPCR was employed to evaluate mRNA levels in the soma. …”
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Mechanical Stress-Induced IGF-1 Facilitates col-I and col-III Synthesis via the IGF-1R/AKT/mTORC1 Signaling Pathway by Bin Yan, Canjun Zeng, Yuhui Chen, Minjun Huang, Na Yao, Jie Zhang, Bo Yan, Jiajun Tang, Liang Wang, Zhongmin Zhang

“…Interestingly, col-I protein, col-I mRNA, col-III protein, col-III mRNA, and IGF-1 protein, but not IGF-1 mRNA, were inhibited by IGF-1 neutralizing antibody. …”
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