Nuclear-Targeting Delivery of CRISPRa System for Upregulation of β-Defensin against Virus Infection by Dexamethasone and Phenylalanine Dual-Modified Dendrimer by Mingxiang Zuo, Xiaoxia Li, Shuang Liu, Bin Chen, Du Cheng

“…The dual-modified dendrimer containing dexamethasone (DET) and phenylalanine (Phe) was prepared to deliver plasmid DNA encoding dCas9 and single-guide RNA (sgRNA) for specific upregulation of β-defensin. …”
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ErbB4 as a Potential Molecular Target in the Treatment of Esophageal Squamous Cell Cancers by Ke Zhao, Bao-Jun Chen, Zhi-Guo Chen

“…The main objective of this study was to examine whether miRNA-140-5p could target and control ErbB4 expression at transcriptional level. …”
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Treatment of Cancer Gene Changes in Chronic Myeloid Leukemia by Big Data Analysis Platform-Based Dasatinib by Linlin Song, Qi Li, Hui Shi, Pengxia Zhang

“…In micro-RNA (miRNA) datasets, there were 202 samples including 1,406 miRNAs. …”
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Non-coding RNAs and exosomal ncRNAs in colorectal cancer by Yalda Zhoulideh

“…In recent years, increasing attention has been paid to the role of non-coding RNA (ncRNA) and exogenous ncRNA in the development and progression of CRC. ncRNAs play an essential role in the regulation of gene expression and cell function, and dysregulation of ncRNA expression is associated with the onset and progression of CRC. …”
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Principles and medical applications of gene editing by CRISPR / Cas by Rolando Sánchez Artigas, María Teresa Díaz Armas, Raisa Rodríguez Duque, Pedro Enrique Miguel Soca

“…Subsequently, the CRISPR sequence is transcribed to generate crRNA and tracrRNA, which form a double-stranded RNA structure that recruits Cas for cleavage. …”
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The role of microRNAs in learning and long-term memory by L. N. Grinkevich

“…MicroRNAs are unique endogenous non-coding RNAs about 22 nucleotides in length; each can regulate translation of hundreds of messenger RNA targets, thereby controlling entire gene networks. …”
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Identification of Differentially Expressed lncRNAs in a CpG ODN-Activated Macrophage by Ying Zheng, Xi Luo, Zailong Qin, Zhiguang Zhou

“…CpG DNA is an immune stimulator recognized by TLR9, subsequently inducing inflammatory responses in macrophages. Long noncoding RNA (lncRNA) is a novel class of noncoding RNA, whose length is more than 200 nt, but without protein-coding capacity. lncRNAs are involved in many physiological and pathological processes, including inflammatory responses. …”
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Caveolin-1 Knockdown Decreases SMMC7721 Human Hepatocellular Carcinoma Cell Invasiveness by Inhibiting Vascular Endothelial Growth Factor-Induced Angiogenesis by Zhi-Bo Zhang, Zheng Shi, Lan-Fang Yang, Hong-Bin Gao

“…Lentiviral vectors were used to construct caveolin-1 small interfering RNA- (siRNA-) expressing cells. Secreted VEGF levels in SMMC7721 cells were evaluated by enzyme-linked immunosorbent assay (ELISA). …”
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In Vitro Study on the Regulation of Annexin IV and VEGF by hCG in the Human Endometrium by Shaoyuan Xu, Jie Li, Xiaoyan Chen, Beiyu Liu

“…Compared with the control group, VEGF mRNA and protein were increased in the hCG group (mRNA/protein: 2.30-fold/1.86-fold), but not in the hCG/PD98059 group. …”
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Proximal Myopathy due to m.5835G>A Mutation in Mitochondrial MT-TY Gene by C. Simoncini, V. Montano, G. Alì, R. Costa, G. Siciliano, M. Mancuso

“…Mitochondrial (mt) tRNA (MTT) gene mutations are an important cause of mitochondrial diseases and are associated with a wide range of clinical presentations. …”
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Assessing the Impact of Cumulus Convection and Turbulence Parameterizations on Typhoon Precipitation Forecast by Yueya Wang, Haobo Li, Xiaoming Shi, Jimmy C. H. Fung

“…The improved forecasting ability is attributed to the RNA's capacity to model dissipation and backscatter. …”
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Comprehensive profiling of serum microRNAs in normal and non-alcoholic fatty liver disease (NAFLD) patients by Jian-Wei Zhang, Kamran Ullah, Nauman Khan, Hai-Tao Pan

“…The pathological mechanisms of NAFLD are linked to functional differences of miRNAs in different biological environments. The miRNA in serum exosomes may reflect the pathological state of the liver and changes in systemic metabolism, while the miRNA in serum may be associated with physiological processes other than the liver. …”
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Adipocyte-specific Steap4 deficiency reduced thermogenesis and energy expenditure in mice by Han Wang, Lizi Zhang, Xing Chen, Lingzi Hong, Junjie Zhao, Wen Qian, Lam Khue Pham, Belinda Willard, Xiaoxia Li, Katarzyna Bulek, Xiao Li

“…Mass spectrometry identified two classes of Steap4 interactomes: mitochondrial proteins and proteins involved in splicing. RNA sequencing (RNA-seq) analysis of white adipose tissue demonstrated that Steap4 deficiency altered RNA splicing patterns with enriched mitochondrial functions. …”
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Genome-Wide Expression Difference of MicroRNAs in Basal Cell Carcinoma by Hai-Peng Wei, Song Zhan, Qing-An Zhu, Zhen-Juan Chen, Xian Feng, Jun-Yuan Chen, Qi-Lin Zhang, Jingjie Zhao, Lingzhang Meng

“…Here, we collected control tissues from adjacent noncancerous skin (n=15; control group) and tissues at tumor centers from patients with cheek BCC (n=15; BCC group) using punch biopsies. After six small RNA sequencing- (sRNA-seq-) based miRNA expression profiles were generated for both BCC and controls, including three biological replicates, we conducted comparative analysis on the sRNA-seq dataset, discovering 181 differentially expressed miRNAs (DEMs) out of the 1,873 miRNAs in BCCs. …”
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Lack of Tissue Virological Response as a Predictor of Relapse in Chronic Hepatitis D after Completion Bulevirtide Therapy by P. O. Bogomolov, N. A. Shub, N. A. Gasilova, A. A. Belyakova, T. G. Lidzhieva, E. A. Kondrasheva, N. A. Barsukova, M. V. Arapova, E. A. Isaeva, S. V. Koblov, M. V. Kalashnikov, O. S. Kuzymina, A. Yu. Demyanov, A. O. Bueverov, I. V. Maev

“…Aim: to evaluate the significance of a positive polymerase chain reaction result for hepatitis D virus RNA (HDV RNA) in liver biopsy specimens of patients with chronic hepatitis D (CHD) after completion of antiviral therapy (AVT) as a predictor of infection relapse.Materials and methods. …”
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Clinical Impact of Viral Load on the Development of Hepatocellular Carcinoma and Liver-Related Mortality in Patients with Hepatitis C Virus Infection by Ran Noh, Doo Hyuck Lee, Byoung Woon Kwon, Yong Hyun Kim, Suk Bae Kim, Il Han Song

“…The cumulative risk of HCC development was higher in subjects with high HCV RNA titer (log HCV RNA IU/mL > 6) than subjects with low titer (log HCV RNA IU/mL ≦ 6) (HR = 4.63, P=0.032), giving an incidence rate of 474.1 and 111.5 per 10,000 person-years, respectively. …”
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Integrated analysis of transcriptome, sRNAome, and degradome involved in the drought-response of maize Zhengdan958 by Yang Shuqiong, Liu Jiafei, Cao Lingling, Chen Jibao, Duan Pengfei

“…Moreover, 6,340 target genes of 445 miRNAs were validated using degradome sequencing, forming 281 miRNA–mRNA pairs in control (CK) and drought-stressed (DS) library. …”
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DHX37 Impacts Prognosis of Hepatocellular Carcinoma and Lung Adenocarcinoma through Immune Infiltration by Yanni Xu, Qiongchao Jiang, Hejun Liu, Xiaoyun Xiao, Dinghong Yang, Phei Er Saw, Baoming Luo

“…Background. RNA helicases have various essential functions in basically all aspects of RNA metabolism, not only unwinding RNA but also disturbing the interaction of RNA with proteins. …”
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Evaluation of reference genes for gene expression analysis in Japanese flounder (Paralichthys olivaceus) under temperature stress by Ping Han, Jianming Chen, Zhennan Sun, Shengjie Ren, Xubo Wang

“…Results In this study, we evaluated the suitability of eight genes including ribosomal protein L6 (rpl6), ribosomal protein L9 (rpl9), delta (4)-desaturase, sphingolipid 1 (degs1), cathepsin L (ctsl), eukaryotic translation elongation factor 1 gamma (eef1g), NSA2 ribosome biogenesis homolog (nsa2), eukaryotic translation initiation factor 3, subunit E, a (eif3ea), glutamine amidotransferase class 1 domain containing 1 (gatd1) analyzed from RNA sequencing (RNA-Seq) data and two genes including β-actin (actb) and 18S rRNA ribosomal RNA (18S RNA) selected from literature to obtain the best internal controls in qRT-PCR analysis of Japanese flounder under temperature stress. …”
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SERS Platform Based on Bimetallic Au-Ag Nanowires-Decorated Filter Paper for Rapid Detection of miR-196ain Lung Cancer Patients Serum by Ji Xia, Yifan Liu, Menglin Ran, Dan Lu, Xiaowei Cao, Youwei Wang

“…Detecting microRNA (miRNA) biomarkers expression is of great significance for the diagnosis and treatment of lung cancer. …”
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