Comprehensive Analysis of Programmed Cell Death-Related Genes in Diagnosis and Synovitis During Osteoarthritis Development: Based on Bulk and Single-Cell RNA Sequencing Data by Zhou J, Jiao S, Huang J, Dai T, Xu Y, Xia D, Feng Z, Chen J, Li Z, Hu L, Meng Q

“…We identified two distinct PCD clusters, each exhibiting unique molecular and immunological characteristics. Single-cell RNA sequencing further revealed dynamic and complex cellular changes in OA synovial tissue, with differential expression of Hub PCD-DEGs across various immune cell types.Conclusion: Our study suggests that PCD-related genes may be involved in development of OA synovitis. …”
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Two complementing in vivo selection systems based on CCA-trimming exonucleases as a tool to monitor, select and evaluate enzymatic features of tRNA nucleotidyltransferases by Karolin Wellner, Josefine Gnauck, Dorian Bernier, Stephan H. Bernhart, Heike Betat, Mario Mörl

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Dihydromyricetin inhibits injury caused by ischemic stroke through the lncRNA SNHG17/miR-452-3p/CXCR4 axis by Jiacheng Xie, Qiuyue Yang, Xueliang Zeng, Qi Zeng, Hai Xiao

“…The interaction between SNHG17 and miR-452-3p was further confirmed by RNA pull-down assays. Furthermore, manipulation of the SNHG17/miR-452-3p/CXCR4 axis was shown to modulate the NF-κB signaling pathway as evidenced by changes in phosphorylation levels. …”
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LncRNA-PVT1 enhances glucose metabolism of pediatric low-grade glioma cells through sponging miR-187-3p by Beibei Li, Zijian Feng, Yanyan Zheng

“…In summary, this study reports critical roles and molecular mechanisms of the lncRNA PVT1-accelarated glucose metabolism of PLGG cells.…”
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lncRNA RUNDC3A-AS1 Regulates Proliferation and Apoptosis of Thyroid Cancer Cells via the miR-151b/SNRPB Axis by Yan Deng, Jie Wu, Xin Li

“…Many previous reports have confirmed that lncRNA is involved in the pathogenesis of THCA. However, the role and mechanism of lncRNA RUNDC3A-AS1 in THCA have not been studied. …”
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METTL14-mediated m6A modification of DDIT4 promotes its mRNA stability in aging-related idiopathic pulmonary fibrosis by Dan Li, Li Qian, Yufeng Du, Lifang Liu, Ziyue Sun, Yongkang Han, Xiangrui Guo, Chao Shen, Zheng Zhang, Xuejun Liu

“…The downregulation of METTL14 might inhibit the decay of DDIT4 mRNA by reducing the m6A modification level of DDIT4 mRNA, leading to high expression of DDIT4 mRNA and protein. …”
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hsa_circRNA_000166 Facilitated Cell Growth and Limited Apoptosis through Targeting miR-326/LASP1 Axis in Colorectal Cancer by Qin Hao, Zhongtao Zhang

“…Then, we confirmed an upregulation of hsa_circRNA_000166 in CRC tissues and cell lines and observed that higher expression of hsa_circRNA_000166 was associated with poor 5-year survival rate of patients with CRC. …”
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Profiling the diversity of the village chicken faecal microbiota using 16S rRNA gene and metagenomic sequencing data to reveal patterns of gut microbiome signatures by Mxolisi Nene, Mxolisi Nene, Nokuthula Winfred Kunene, Rian Pierneef, Rian Pierneef, Rian Pierneef, Khanyisile Hadebe

“…This study aimed to investigate the diversity and functional capacities of the faecal microbiome in village chickens from Limpopo and KwaZulu-Natal provinces of South Africa.MethodsUsing a combination of 16S rRNA gene sequencing and shotgun metagenomic sequencing technologies, we analysed 98 16S rRNA and 72 metagenomic datasets. …”
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Overcoming breast cancer cell treatment resistance by optimizing sonodynamic therapy and radiation sensitizers on lncRNA PVT1 and miR-1204 expression by Ali Neshastehriz, Zeinab Hormozi-Moghaddam, Zahra Abedi Kichi, Seyedeh Mona Taheri, Seyed Mohammad Amini, Amir Aghaei

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Soil Acidobacterial 16S rRNA Gene Sequences Reveal Subgroup Level Differences between Savanna-Like Cerrado and Atlantic Forest Brazilian Biomes by Elisa C. P. Catão, Fabyano A. C. Lopes, Janaína F. Araújo, Alinne P. de Castro, Cristine C. Barreto, Mercedes M. C. Bustamante, Betania F. Quirino, Ricardo H. Krüger

“…Using 16S rRNA sequences, the present work aimed to study acidobacterial diversity and distribution in soils of Cerrado savanna and two Atlantic forest sites. …”
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Association of HIVEP3 Gene and Lnc RNA with Femoral Neck Bone Mineral Content and Hip Geometry by Genome-Wide Association Analysis in Chinese People by Weiwei Hu, Jinwei He, Luyue Qi, Chun Wang, Hua Yue, Jiemei Gu, Hao Zhang, Yi Wang, Zhenlin Zhang

“…Through this study, we found that HIVEP3 gene and LINC RNA are potentially correlated with femoral neck BMC. …”
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Branched endosomal disruptor (BEND) lipids mediate delivery of mRNA and CRISPR-Cas9 ribonucleoprotein complex for hepatic gene editing and T cell engineering by Marshall S. Padilla, Kaitlin Mrksich, Yiming Wang, Rebecca M. Haley, Jacqueline J. Li, Emily L. Han, Rakan El-Mayta, Emily H. Kim, Sofia Dias, Ningqiang Gong, Sridatta V. Teerdhala, Xuexiang Han, Vivek Chowdhary, Lulu Xue, Zain Siddiqui, Hannah M. Yamagata, Dongyoon Kim, Il-Chul Yoon, James M. Wilson, Ravi Radhakrishnan, Michael J. Mitchell

“…This work provides a scheme to generate a class of ILs for both mRNA and protein delivery.…”
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Leukemia Stem Cell-Released Microvesicles Promote the Survival and Migration of Myeloid Leukemia Cells and These Effects Can Be Inhibited by MicroRNA34a Overexpression by Yue Wang, Qian Cheng, Jing Liu, Min Dong

“…LSCs were transfected with miRNA control (miRCtrl) or miR34a mimic for producing LMVs, respectively, defined as LMVsmiRCtrl and LMVsmiR34a. …”
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The Effect of P-Cymene on Glucose, Insulin, Antioxidant Power, and the Level of AKT Gene mRNA in Streptozotocin-Induced Diabetes Model in Male Wistar Rats by Maryam Arabloei Sani, Zahra Hajebrahimi, Parichehreh Yaghmaei, Nasim Hayati Roodbari

“…Administration of metformin or p-cymene improved the levels of glucose, insulin, AKT mRNA, β island size, and antioxidant power in an independent manner of dose. …”
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Immunogenicity of Pfizer mRNA COVID-19 Vaccination Followed by J&J Adenovirus COVID-19 Vaccination in Two Patients with Chronic Lymphocytic Leukemia by Zoe L. Lyski, Myung Sun Kim, David Xthona Lee, David Sampson, Hans-Peter Raué, Vikram Raghunathan, Debbie Ryan, Amanda E. Brunton, Mark K. Slifka, William B. Messer, Stephen E. Spurgeon

“…The results of this study, however small, are especially promising to CLL individuals who did not seroconvert following the initial mRNA vaccination series.…”
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Limited Diagnostic Value of miRNAs in Early Trauma-Induced Liver Injury: Only miRNA-122 Emerges as a Late-Phase Marker by Jason-Alexander Hörauf, Amit Singh, Maika Voth, Hamed Moheimani, Cora Rebecca Schindler, Borna Relja, Liudmila Leppik, Ingo Marzi, Dirk Henrich

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Machine Learning-Devised Immune-Related lncRNA Signature Panel Predicts the Prognosis and Immune Landscape in Breast Cancer Novel IRLP Signature in BRCA by Jun-Yu Zhu, An-Qi Lyu, Zhang-Ting Wang, Wai-Yee Chan, Tao Qin, Kai-Kei Miu, He-Rui Yao

“…Our study identified immune-related lncRNA (irlncRNA) pairs and compiled them into a set of noncoding gene signatures able to stratify subtypes of BRCA associated with variable degrees of survival and immune cell infiltration. …”
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Ameliorative Effect of Hovenia dulcis Fruit Pedicel Polysaccharides on Alcohol-Induced Intestinal Injury in Mice Investigated Using 16S rRNA Sequencing and Metabolomics by ZHANG Yuchao, ZHU Sijie, LIU Liangyu, WANG Jianyun, ZHANG Jiaxin, SU Zexiong, CHEN Jiangyan, LIU Xudong

“…Using high-throughput 16S rRNA gene sequencing technology and metabolomics, we investigated the impacts of HDPs on the gut microbiota and metabolic profile of mice with alcohol-induced intestinal injury. …”
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A new strategy for pharmacodynamic substance screening and research on gut microbiota pathway mechanisms based on UPLC-Q-orbitrap-MS and 16S rRNA by Zhiying Yu, Tong Li, Jie Yang, Jianghua He, Weijiang Zhang, Siyuan Li, Yunpeng Qi, Yihui Yin, Ling Dong, Wenjuan Xu

“…To analyze the structure and function of characteristic genera of GQD and its active components, 16S rRNA sequencing was performed. Results: We identified 67 prototypic and 14 metabolic components of GQD in the UC colon. …”
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scRNA seq of an F1 cross of Marek’s disease resistant and susceptible chickens identifies allele specific expression signatures enriched in transcription modulators by Deborah Velez-Irizarry, Hans Cheng, Cari Hearn

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