Epithelial and immune transcriptomic characteristics and possible regulatory mechanisms in asthma exacerbation: insights from integrated studies by Ye Liu, Yue Li, Ruhao Wu, Yu Wang, Pengfei Li, Tianci Jiang, Ke Wang, Yize Liu, Zhe Cheng

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Analysis of somatic piRNAs in the malaria mosquito Anopheles coluzzii reveals atypical classes of genic small RNAs by Sergei Funikov, Alexander Rezvykh, Natalia Akulenko, Jiangtao Liang, Igor V. Sharakhov, Alla Kalmykova

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Detection of Cancer Stem Cells from Patient Samples by Sofia Hakala, Anna Hämäläinen, Sanne Sandelin, Nikolaos Giannareas, Elisa Närvä

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Profiling of small RNAs derived from tomato brown rugose fruit virus in infected Solanum lycopersicum plants by deep sequencing by Mangle Chen, Donghai Wang, Jiali Yang, Yuhao Cao, Xuemei Song, Yuwen Lu, Hongying Zheng, Jiejun Peng, Guanwei Wu, Jian Wu, Junmin Li, Jianping Chen, Fei Yan, Shaofei Rao

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RNA Interference Targeting Snail Inhibits the Transforming Growth Factor β2-Induced Epithelial-Mesenchymal Transition in Human Lens Epithelial Cells by Ping Li, Jiaona Jing, Jianyan Hu, Tiejun Li, Yuncheng Sun, Huaijin Guan

“…This study was done to investigate the effect of Snail targeting siRNA on TGFβ2-induced EMT in human lens epithelial cells. …”
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Endothelial Cell-Derived Extracellular Vesicles Target TLR4 via miRNA-326-3p to Regulate Skin Fibroblasts Senescence by Xinni Yang, Jiyong Tan, Jiqing Shen, Xin Zhang, Gaoxiang Huang, Xiaoxue Su, Jing Li

“…Western blot was used to detect TLR4 protein expression. The target gene of miRNA were identified using a double luciferase reporter assay. miR-326-3p mimic/inhibitor and siRNA-TLR4 can demonstrate a nonnegligible link between miR-326-3p-TLR4 and skin aging. …”
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A CRISPR-Cas12a-based universal rapid scrub typhus diagnostic method targeting 16S rRNA of Orientia tsutsugamushi. by Bum Ju Park, Sang Taek Heo, Misun Kim, Jeong Rae Yoo, Eun Jin Bae, Su Yeon Kang, Sunghoon Park, Kyeo Re Han, Keun Hwa Lee, Jae Myun Lee, Hyeyoung Lee, Yoon-Jae Song

“…Here, we developed a CRISPR-Cas12a-based diagnostic method targeting the bacterial 16S rRNA to detect O. tsutsugamushi infection of all known genotypes. …”
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lncRNA-KCNQ1OT1: A Potential Target in Exosomes Derived from Adipose-Derived Stem Cells for the Treatment of Osteoporosis by Shan-zheng Wang, Jun Jia, Chang-hong Chen

“…This study was aimed at investigating the role of ADSCs-Exos and a novel long noncoding RNA KCNQ1OT1 played in osteoporosis as well as the underlying mechanism. …”
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The Effect of Lysophosphatidic Acid during In Vitro Maturation of Bovine Oocytes: Embryonic Development and mRNA Abundances of Genes Involved in Apoptosis and Oocyte Competence by Dorota Boruszewska, Ana Catarina Torres, Ilona Kowalczyk-Zieba, Patricia Diniz, Mariana Batista, Luis Lopes-da-Costa, Izabela Woclawek-Potocka

“…Supplementation of maturation medium with LPA increased mRNA abundance of FST and GDF9 in oocytes and decreased mRNA abundance of CTSs in cumulus cells. …”
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A Novel Approach for In Vitro Testing and Hazard Evaluation of Nanoformulated RyR2-Targeting siRNA Drugs Using Human PBMCs by Valeria Bettinsoli, Gloria Melzi, Angelica Crea, Lorenzo Degli Esposti, Michele Iafisco, Daniele Catalucci, Paolo Ciana, Emanuela Corsini

“…As proof of concept, a small interfering RNA (siRNA) targeting Ryanodine Receptor mRNA (RyR2) identified by the Italian National Center for Gene Therapy and Drugs based on RNA Technology as a potential therapeutic target for dominant catecholaminergic polymorphic ventricular tachycardia, was selected. …”
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Isolation and Molecular Identification of Lactic Acid Bacteria Using 16s rRNA Genes from Fermented Teff (Eragrostis tef (Zucc.)) Dough by Belay Tilahun, Anteneh Tesfaye, Diriba Muleta, Andualem Bahiru, Zewdu Terefework, Gary Wessel

“…Genomic DNA of bacterial isolates were extracted using Invisorb® Spin DNA Extraction kit. 16S rRNA of bacterial isolates were amplified using the bacteria universal primers (rD1 and fD1). …”
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MicroRNA-325-3p Targets Human Epididymis Protein 4 to Relieve Right Ventricular Fibrosis in Rats with Pulmonary Arterial Hypertension by Yi Tang, Xiaowei Huo, Junyu Liu, Yijin Tang, Min Zhang, Wenlin Xie, Zhaofen Zheng, Jin He, Jiayan Lian

“…Our purpose was to explore microRNA-325-3p (miR-325-3p)/human epididymis protein 4’s (HE4) role in the occurrence and development of right ventricular fibrosis in PAH. …”
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Cucurbitacin IIa Alleviates Colitis via Promoting the Release of Host-Derived Extracellular Vesicles Encapsulating microRNA-30b-5p by Zhao Y, Jiang B, Zuo S

“…Specifically, microRNA-30b-5p, significantly increased by cucurbitacin IIa, was found to alleviate colitis symptoms in DSS-treated rats. …”
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Combined analysis of 16S rRNA sequencing and metabolomics reveals the growth-promoting mechanism of compound probiotics in zig-zag eel (Mastacembelus armatus) by Yuwei Feng, Zhiting Zuo, Guangting Xie, Yiman Chen, Xiaoli Yin, Baoyue Lu, Zhide Yu, Chong Han, Hu Shu

“…The intestinal physiology was reflected by intestinal histology, intestinal digestive enzyme, and antioxidant enzyme activity (P < 0.05). 16S rRNA sequencing was used to analyze the gut microbiota of different groups. …”
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The piRNA protein Asz1 is essential for germ cell and gonad development in zebrafish and exhibits differential necessities in distinct types of germ granules. by Adam Ahmad, Yoel Bogoch, Gal Shvaizer, Noga Guler, Karine Levy, Yaniv M Elkouby

“…Germline development requires distinct types of germ granules, which contains RNA-protein (RNP) complexes, including germ plasm in embryos, piRNA granules in gonadal germ cells, and the Balbiani body (Bb) in oocytes. …”
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Unravelling the transcriptomic symphony of muscle ageing: key pathways and hub genes altered by ageing and caloric restriction in rat muscle revealed by RNA sequencing by Gulam Altab, Brian J. Merry, Charles W. Beckett, Priyanka Raina, Inês Lopes, Katarzyna Goljanek-Whysall, João Pedro de Magalhães

“…RNA-seq data were subjected to Gene Ontology, pathway, co-expression, and interaction network analyses. …”
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The human‐specific noncoding RNA RP11‐424G14.1 functions at the intersection of sexually dimorphic pathways in inflammation, senescence, and metabolism by Kameron Kennicott, Yun Liang

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Metformin Exhibits Anti‐Inflammatory Effects by Regulating microRNA‐451/CXCL16 and B Cell Leukemia/Lymphoma 2 in Patients With Osteoarthritis by Nahid Alimoradi, Amin Ramezani, Mohammad Tahami, Negar Firouzabadi

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Heterogeneous nuclear ribonucleoprotein C promotes non-small cell lung cancer progression by enhancing XB130 mRNA stability and translation by Qinrong Wang, Xuanjing Gou, Lingling Liu, Daolan Deng, Yan Zhao, Jianjiang Zhou, Yuan Xie, Yinhui Jiang, Jianglun Li, Jian Zhang, Ying Liu

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Dexmedetomidine Mitigates Microglial Activation Associated with Postoperative Cognitive Dysfunction by Modulating the MicroRNA-103a-3p/VAMP1 Axis by Zhichao Wu, Han Wang, Zuan Shi, Yalan Li

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