Comprehensive analyses reveal the promising value of gasdermins as prognostic biomarkers and immunotherapeutic targets in head and neck squamous cell carcinoma by Huageng Huang, Jingjing Ge, Shunzhen Lu, Xinyi Deng, Ying Tian, He Huang, Zhao Wang, Yuyi Yao, Huangming Hong, Tongyu Lin

“…Compared with those in normal tissues, the mRNA and protein levels of GSDMs, especially GSDMD/E, were commonly elevated in HNSC (P < 0.05). …”
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Streptococcus intermedius promotes synchronous multiple primary lung cancer progression through apoptosis regulation by Yi Deng, Yi Deng, Yi Deng, Yi Deng, Zhi Xiang Dong, Zhi Xiang Dong, Gao Hua Yang, William S. Krimsky, Yong Hang Tai, Hao Peng, Hao Peng, Hao Peng, Hao Peng, Gui Ting Huang, Gui Ting Huang, Gui Ting Huang, Jia Xin Xu, Jia Xin Xu, Jia Xin Xu, Saiyad A. Sarkar, Jun Peng, Jun Peng, Jun Peng, Jun Peng, Kai Qian, Kai Qian, Kai Qian, Kai Qian

“…However, the relationship between sMPLC pathogenesis and changes in the lung microbiome remains unclear.MethodsIn this study, 16S rRNA sequencing was performed on clinical samples to analyze lung microbiome composition. …”
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Isolation and Characterization of Indigenous <i>Acetobacter</i> Strains from Cashew Apple and Their Potential Use in Vinegar Production by Yaya Anianhou Ouattara, Doudjo Soro, Kouakou Romain Fossou, Ahou Cinthia Ines Yebouet, Ismael Abraham Cissé, Dogbo Marius Akissi, Adolphe Zézé, Emmanuel Assidjo, Kouassi Benjamin Yao

“…Physiological and biochemical tests followed by 16S rRNA gene analysis and phylogeny were used for isolate characterization. …”
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Bone Marrow Mesenchymal Stem Cells-Derived Exosomes Inhibit Apoptosis of Pulmonary Microvascular Endothelial Cells in COPD Mice Through miR-30b/Wnt5a Pathway by Song Q, Zhou A, Cheng W, Zhao Y, Liu C, Zeng Y, Lin L, Zhou Z, Peng Y, Chen P

“…Qing Song,1– 4 Aiyuan Zhou,5 Wei Cheng,1– 4 Yiyang Zhao,6 Cong Liu,1– 4 Yuqin Zeng,1– 4 Ling Lin,1– 4 Zijing Zhou,1– 4 Yating Peng,1– 4 Ping Chen1– 4 1Department of Respiratory and Critical Care Medicine, the Second Xiangya Hospital, Central South University, Changsha, Hunan, 410011, People’s Republic of China; 2Clinical Medical Research Center for Pulmonary and Critical Care Medicine in Hunan Province, Changsha, 410011, People’s Republic of China; 3Diagnosis and Treatment Center of Respiratory Disease, Central South University, Changsha, Hunan, 410011, People’s Republic of China; 4Research Unit of Respiratory Disease, Central South University, Changsha, Hunan, 410011, People’s Republic of China; 5Department of Respiratory and Critical Care Medicine, the Xiangya Hospital of Central South University, Changsha, Hunan, 410011, People’s Republic of China; 6Ultrasound Imaging Department, Xiangya Hospital of Central South University, Changsha, Hunan, 410083, People’s Republic of ChinaCorrespondence: Ping Chen, Department of Respiratory and Critical Care Medicine, the Second Xiangya Hospital, Central South University, Renmin Middle Road, Changsha, Hunan, 410011, People’s Republic of China, Tel 13873115563, Email pingchen0731@csu.edu.cnBackground: Bone marrow mesenchymal stem cells (BMSCs)-derived exosomes are rich in a variety of active substances, including microRNA (miR) and have shown powerful therapeutic effects to ameliorate cell injury and diseases. …”
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Biologically targeted discovery-replication scan identifies G×G interaction in relation to risk of Barrett’s esophagus and esophageal adenocarcinoma by Li Yan, Qianchuan He, Shiv P. Verma, Xu Zhang, Ann-Sophie Giel, Carlo Maj, Kathryn Graz, Elnaz Naderi, Jianhong Chen, Mourad Wagdy Ali, Puya Gharahkhani, Xiang Shu, Kenneth Offit, Pari M. Shah, Hans Gerdes, Daniela Molena, Amitabh Srivastava, Stuart MacGregor, Claire Palles, René Thieme, Michael Vieth, Ines Gockel, Thomas L. Vaughan, Johannes Schumacher, Matthew F. Buas

“…Rs3217992 maps to the CDKN2B 3′ UTR and reportedly disrupts microRNA-mediated repression. Rs17744726 maps to an intronic enhancer region in BLK. …”
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Xixin Decoction’s novel mechanism for alleviating Alzheimer’s disease cognitive dysfunction by modulating amyloid-β transport across the blood–brain barrier to reduce neuroinflamma... by Chaokai Yang, Enlong Zhao, Hu Zhang, Liqi Duan, Xinyue Han, Hongli Ding, Yan Cheng, Dengkun Wang, Dengkun Wang, Dengkun Wang, Xiaojing Lei, Xiaojing Lei, Xiaojing Lei, Yongchang Diwu, Yongchang Diwu, Yongchang Diwu

“…BBB integrity was evaluated using Evans blue staining, and protein expression levels were analyzed via ELISA, Western blotting, and immunofluorescence.ResultsIn vitro experiments revealed that XXD-containing serum, when cultured for 24, 48, and 72 h, could upregulate the expression of P-gp mRNA and protein, downregulate CB1 protein expression, and upregulate CB2 and Mfsd2a protein expression. …”
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Age-related calcium signaling disturbance restricted cAMP metabolism and induced ovarian oxidation stress in laying ducks by Cheng-long Jin, Sheng-lin Wang, Shuang Wang, Ya-nan Zhang, Wei-guang Xia, Chang Zhang, Xue-bing Huang, Kai-chao Li, Chun-tian Zheng, Wei Chen

“…We found the poor productive performance of aged ducks was correlated with follicle decreased numbers and atrophied microstructure, and restricted antioxidant ability of granulosa cells (GCs). Then, according to RNA-Seq analysis, we detected those aged ducks displayed lower Ca concentration in the ovary, while Ca channel related gene expression was increased in GCs to maintain homeostasis. …”
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Galactin-8 DNA methylation mediates macrophage autophagy through the MAPK/mTOR pathway to alleviate atherosclerosis by Bing Xia, Yan-lin Lu, Jin Peng, Jing-wei Liang, Fang-qin Li, Jiu-yang Ding, Chang-wu Wan, Cui-yun Le, Jia-lin Dai, Jie-Wang, Bing Guo, Jiang Huang

“…After the specific knockdown of GAL-8 using siRNA GAL-8 using foam cells, the above phenomenon was reversed, macrophage autophagy was promoted, the inflammatory response was reduced, and the degree of atherosclerosis was alleviated. …”
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Intervention effect and mechanism of Dracocephalum moldavica L. extract on bleomycin-induced pulmonary fibrosis in rats by Xiaoyu SUN, Li CHEN, Ruifang GAO, Hui YU, Min JIN

“…Real-time fluorescence quantitative PCR (RT-qPCR) was used to detect α-SMA and Col-I mRNA levels in lung tissue. Enzyme-linked immunosorbent assay (ELISA) was used to detect tumour necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β) in rats. …”
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Genome-Wide Analysis, Characterization, and Expression Profile of the Basic Leucine Zipper Transcription Factor Family in Pineapple by Yanhui Liu, Mengnan Chai, Man Zhang, Qing He, Zhenxia Su, S. V. G. N. Priyadarshani, Liping Liu, Guanxi Dong, Yuan Qin

“…The study also analyzed the pineapple gene expression of bZIP genes in different tissues, organs, and developmental stages, as well as in abiotic stress responses. The RNA-sequencing data showed that AcobZIP57 was upregulated in all tissues, including vegetative and reproductive tissues. …”
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Characteristics and clinical significance of immune cells in omental milky spots of patients with gastric cancer by Yasunobu Mano, Yasunobu Mano, Yuka Igarashi, Yuka Igarashi, Keisuke Komori, Keisuke Komori, Itaru Hashimoto, Itaru Hashimoto, Hayato Watanabe, Kosuke Takahashi, Kazuki Kano, Hirohito Fujikawa, Takanobu Yamada, Hidetomo Himuro, Hidetomo Himuro, Taku Kouro, Taku Kouro, Feifei Wei, Feifei Wei, Kayoko Tsuji, Kayoko Tsuji, Shun Horaguchi, Shun Horaguchi, Shun Horaguchi, Mitsuru Komahashi, Mitsuru Komahashi, Mitsuru Komahashi, Takashi Oshima, Tetsuro Sasada, Tetsuro Sasada

“…In this study, we investigated gene expression profiles in cells from omental milky spots of patients with gastric cancer (n = 37) by RNA sequencing analysis and classified the patients into four groups (G1-4). …”
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Landscape of respiratory syncytial virus by Yuping Duan, Zimeng Liu, Na Zang, Bingbing Cong, Yuqing Shi, Lili Xu, Mingyue Jiang, Peixin Wang, Jing Zou, Han Zhang, Ziheng Feng, Luzhao Feng, Lili Ren, Enmei Liu, You Li, Yan Zhang, Zhengde Xie, Jing Ni

“…Respiratory syncytial virus (RSV) is an enveloped, negative-sense, single-stranded RNA virus of the Orthopneumovirus genus of the Pneumoviridae family in the order Mononegavirales. …”
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Gut microbiota drives colon cancer risk associated with diet: a comparative analysis of meat-based and pesco-vegetarian diets by Carlotta De Filippo, Sofia Chioccioli, Niccolò Meriggi, Antonio Dario Troise, Francesco Vitali, Mariela Mejia Monroy, Serdar Özsezen, Katia Tortora, Aurélie Balvay, Claire Maudet, Nathalie Naud, Edwin Fouché, Charline Buisson, Jacques Dupuy, Valérie Bézirard, Sylvie Chevolleau, Valérie Tondereau, Vassilia Theodorou, Claire Maslo, Perrine Aubry, Camille Etienne, Lisa Giovannelli, Vincenzo Longo, Andrea Scaloni, Duccio Cavalieri, Jildau Bouwman, Fabrice Pierre, Philippe Gérard, Françoise Guéraud, Giovanna Caderni

“…We analysed multiple tumour markers and assessed the variations in the faecal microbiota using 16S rRNA gene sequencing together with targeted- and untargeted-metabolomics analyses. …”
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Solid Predominant Histology and High Podoplanin Expression in Cancer-Associated Fibroblast Predict Primary Resistance to Osimertinib in EGFR-Mutated Lung Adenocarcinoma by Yuji Uehara, MD, Hiroki Izumi, MD, PhD, Tetsuro Taki, MD, PhD, Tetsuya Sakai, MD, PhD, Hibiki Udagawa, MD, PhD, Eri Sugiyama, MD, PhD, Shigeki Umemura, MD, PhD, Yoshitaka Zenke, MD, PhD, Shingo Matsumoto, MD, PhD, Kiyotaka Yoh, MD, PhD, Shoko Kubota, MD, PhD, Keiju Aokage, MD, PhD, Naoya Sakamoto, MD, PhD, Shingo Sakashita, MD, PhD, Motohiro Kojima, MD, PhD, Michiko Nagamine, MD, PhD, Yukio Hosomi, MD, PhD, Masahiro Tsuboi, MD, PhD, Koichi Goto, MD, PhD, Genichiro Ishii, MD, PhD

“…We also investigated HGF mRNA expression levels, using The Cancer Genome Atlas Program and Singapore Oncology Data Portal cohorts. …”
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Revealing the biological features of the axolotl pancreas as a new research model by Hui Ma, Hui Ma, Hui Ma, Guangcong Peng, Yan Hu, Binbin Lu, Binbin Lu, Yiying Zheng, Yingxian Wu, Weimin Feng, Yu Shi, Xiangyu Pan, Li Song, Ina Stützer, Yanmei Liu, Jifeng Fei, Jifeng Fei, Jifeng Fei

“…Pancreatic development was observed using in situ hybridization and immunostaining for key pancreatic markers. RNA sequencing was performed to profile global gene expression during larva and adult stages. …”
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颈项透明层增厚胎儿的产前诊断及预后分析

“…【方法】 对１３５例妊娠早期（１１～１３＋６周）ＮＴ≥２．５ ｍｍ且行产前诊断的单胎病例进行回顾性分析，统计胎儿染色体核型、ＴＯＲＣＨ－ＤＮＡ／ＲＮＡ、地中海贫血基因检测结果，随访胎儿超声筛查结构异常情况及妊娠结局。按ＮＴ厚度将其依次分为５组，２．５～２．９、３．０～３．４、３．５～４．４、≥４．５ ｍｍ为第１～４组，颈部水囊瘤为第５组。…”
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Hyperthermia suppresses the biological characteristics and migration of chicken primordial germ cells by Yuzhou Gu, Yuzhou Gu, Yuzhou Gu, Kexin Wu, Kexin Wu, Kexin Wu, Bowen Niu, Bowen Niu, Bowen Niu, Zhiting Wang, Zhiting Wang, Zhiting Wang, Yuchen Jie, Yuchen Jie, Yuchen Jie, Zixuan Fan, Zixuan Fan, Zixuan Fan, Junying Li, Junying Li, Congjiao Sun, Congjiao Sun, Congjiao Sun, Zhuo-Cheng Hou, Zhuo-Cheng Hou, Zhuo-Cheng Hou, Li-Wa Shao, Li-Wa Shao, Li-Wa Shao

“…Cell viability, glycogen granule contents, and mRNA expression levels of pluripotency markers (NANOG and POUV), germ cell-specific markers (DAZL and CVH), and telomerase reverse transcriptase (TERT) were reduced in PGCs cultured under high temperatures (43°C for 12, 24, and 48 h). …”
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The process of nitrogen-adaptation root endophytic bacterial rather than phosphorus-adaptation fungal subcommunities construction unveiled the tomato yield improvement under long-t... by Xiaoxia Li, Xiaoxia Li, Xiaoxia Li, Muhammad Awais, Muhammad Awais, Muhammad Awais, Shuang Wang, Shuang Wang, Shuang Wang, Shuang Wang, Zhu Zhang, Zhu Zhang, Zhu Zhang, Shuning Zhao, Shuning Zhao, Shuning Zhao, Yufeng Liu, Yufeng Liu, Yufeng Liu, Zhouping Sun, Zhouping Sun, Zhouping Sun, Hongdan Fu, Hongdan Fu, Hongdan Fu, Tianlai Li, Tianlai Li, Tianlai Li

“…We studied the 26th year of a field experiment conducted in a greenhouse with varying levels of nitrogen and phosphorus (CKP0, CKP1, CNP0, CNP1, ONP0, and ONP1) to assess the composition of tomato root endophytes and their impact on yield. We employed 16S rRNA and fungal ITS region amplicon sequencing to investigate the assembly mechanisms of abundant and rare endophytic subcommunities, network correlations, core subcommunity structures, and key species that enhance crop yield. …”
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Immune and genomic biomarkers of immunotherapy response in cancer of unknown primary by Madhu Singh, Linda Mileshkin, Penelope Schofield, Bo Gao, Gary Richardson, Andrew Pattison, Rodney J Hicks, Niko Thio, Mark Warren, Colin Wood, Stephen B Fox, Anna DeFazio, Andrew Fellowes, Christopher Steer, David Bowtell, Nicholas Wilcken, Tharani Sivakumaran, Atara Posner, Dariush Etemadmoghadam, Krista Fisher, Samantha Webb, Christos S Karapetis, Ian M Collins, Narayan Karanth, Owen W J Prall, Richard William Tothill

“…Tumor mutation burden (TMB) and gene mutations were also assessed.Results A total of 219 patients with CUP were included, 215 assessed for TOO in a previous study, with the majority (163) receiving both RNA and DNA tests. Of GEP profiled cases, 33% (59/175) had a high IR gene-expression score. …”
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Dual specific STAT3/5 degraders effectively block acute myeloid leukemia and natural killer/T cell lymphoma by Daniel Pölöske, Helena Sorger, Anna Schönbichler, Elvin D. deAraujo, Heidi A. Neubauer, Anna Orlova, Sanna H. Timonen, Diaaeldin I. Abdallah, Aleksandr Ianevski, Heikki Kuusanmäki, Marta Surbek, Elisabeth Heyes, Thomas Eder, Christina Wagner, Tobias Suske, Martin L. Metzelder, Michael Bergmann, Maik Dahlhoff, Florian Grebien, Roman Fleck, Christine Pirker, Walter Berger, Emir Hadzijusufovic, Wolfgang R. Sperr, Lukas Kenner, Peter Valent, Tero Aittokallio, Marco Herling, Satu Mustjoki, Patrick T. Gunning, Richard Moriggl

“…JPX‐0700/−0750 decreased the mRNA and protein levels of STAT3/5 targets involved in cancer survival, metabolism, and cell cycle progression, exhibiting nanomolar to low micromolar efficacy. …”
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