GH inhibits ALV-J replication and restricts cell cycle by activating PI3K/Akt signaling pathway by Junliang Xia, Ling Lin, Rongyang Ju, Chengxun Xu, Guodong Mo, Xiquan Zhang

“…In this study, we observed a significant upregulation of GH protein expression in the plasma of ALV infected chickens, and a marked increase in GH mRNA in ALV-J infected cells. We found that lower gp85 expression correlated with higher GH expression in immune tissues, suggesting that GH may inhibit gp85 expression. …”
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Ucp1 Ablation Improves Skeletal Muscle Glycolytic Function in Aging Mice by Jin Qiu, Yuhan Guo, Xiaozhen Guo, Ziqi Liu, Zixuan Li, Jun Zhang, Yutang Cao, Jiaqi Li, Shuwu Yu, Sainan Xu, Juntong Chen, Dongmei Wang, Jian Yu, Mingwei Guo, Wenhao Zhou, Sainan Wang, Yiwen Wang, Xinran Ma, Cen Xie, Lingyan Xu

“…To understand the potential crosstalk, RNA‐seq and metabolomic analysis were performed on adipose tissue and muscle in aging mice and revealed that creatine levels are increased both in iWAT and muscle of UCP1 KO mice. …”
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Multiparametric MRI along with machine learning predicts prognosis and treatment response in pediatric low-grade glioma by Anahita Fathi Kazerooni, Adam Kraya, Komal S. Rathi, Meen Chul Kim, Arastoo Vossough, Nastaran Khalili, Ariana M. Familiar, Deep Gandhi, Neda Khalili, Varun Kesherwani, Debanjan Haldar, Hannah Anderson, Run Jin, Aria Mahtabfar, Sina Bagheri, Yiran Guo, Qi Li, Xiaoyan Huang, Yuankun Zhu, Alex Sickler, Matthew R. Lueder, Saksham Phul, Mateusz Koptyra, Phillip B. Storm, Jeffrey B. Ware, Yuanquan Song, Christos Davatzikos, Jessica B. Foster, Sabine Mueller, Michael J. Fisher, Adam C. Resnick, Ali Nabavizadeh

“…Here, we present a radiogenomic analysis of pLGGs, integrating MRI and RNA sequencing data. We identify three immunologically distinct clusters, with one group characterized by increased immune activity and poorer prognosis, indicating potential benefit from immunotherapies. …”
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Loss of MEF2C function by enhancer mutation leads to neuronal mitochondria dysfunction and motor deficits in mice by Ali Yousefian-Jazi, Suhyun Kim, Jiyeon Chu, Seung-Hye Choi, Phuong Thi Thanh Nguyen, Uiyeol Park, Min-gyeong Kim, Hongik Hwang, Kyungeun Lee, Yeyun Kim, Seung Jae Hyeon, Hyewhon Rhim, Hannah L. Ryu, Grewo Lim, Thor D. Stein, Kayeong Lim, Hoon Ryu, Junghee Lee

“…To verify the role of MEF2C-knockdown (MEF2C-KD) in mice, we developed AAV expressing shRNA for MEF2C based on AAV-U6 promoter vector. Neuropathological alterations of MEF2C-KD mice with mitochondrial dysfunction and motor neuronal damage were observed by confocal microscopy and transmission electron microscope (TEM). …”
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Periostin-mediated NOTCH1 activation between tumor cells and HSCs crosstalk promotes liver metastasis of small cell lung cancer by Linlin Lou, Keren Peng, Shumin Ouyang, Wen Ding, Jianshan Mo, Jiayu Yan, Xiaoxiao Gong, Guopin Liu, Jinjian Lu, Peibin Yue, Kai Zhang, Jian Zhang, Yan-dong Wang, Xiao-lei Zhang

“…Cell migration, invasion and sphere formation assay were performed to determine the oncogenic role of POSTN. RNA sequencing analysis was utilized to identify the key signaling pathway regulated by POSTN. …”
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The involvement of CgCaspase-3 in regulating the expressions of proinflammatory cytokines through NF-κB/Rel signaling pathway in oysters by Zihan Wang, Xiaopeng Li, Jiejie Sun, Shuyi Mu, Wei Wu, Renle Chang, Lingling Wang, Linsheng Song

“…In haemocytes of Ac-DEVD-CHO-treated and CgCaspase-3-RNAi oysters, the nuclear translocation of NF-κB/Rel was inhibited at 24 h after Vibrio splendidus stimulation. The mRNA expressions of interleukins 17s (CgIL17–2, CgIL17–3, and CgIL17–6), tumor necrosis factor-1 (CgTNF-1), high mobility group box-1 protein (CgHMGB1) and interferon-like protein (CgIFNLP) were significantly decreased in haemocytes of CgCaspase-3-RNAi or CgRel-RNAi oysters, respectively. …”
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Iodine activates NLRP3 inflammasomes in PBMCs of patients with autoimmune thyroiditis and regulates Th1 and Th17 cell differentiation by Ying Wu, Qingling Guo, Yongping Liu, Xun Gong, Wei Sun, Yushu Li, Chenling Fan, Weiping Teng, Zhongyan Shan

“…Methods: We collected PBMCs and serum samples from 50 patients with AIT with normal thyroid function and 50 controls matched for age and sex. In PBMCs, the mRNA and protein expressions of certain inflammasome constituents (e.g., NLRP1, NLRP3, absent in melanoma 2 (AIM2) and caspase-1), interleukin (IL)-1β and IL-18 were assessed using qRT-PCR and western blotting. …”
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EZH2/miR-142-3p/HMGB1 axis mediates chondrocyte pyroptosis by regulating endoplasmic reticulum stress in knee osteoarthritis by Yang Chen, Shanshan Dong, Xin Zeng, Qing Xu, Mingwei Liang, Guangneng Liao, Lan Li, Bin Shen, Yanrong Lu, Haibo Si, Peifang Wei

“…The expression of the histone methyltransferase enhancer of zeste homolog 2 (EZH2), microRNA-142-3p (miR-142-3p), and high mobility group box 1 (HMGB1) and the levels of ER stress, pyroptosis, and metabolic markers in normal and OA chondrocytes were investigated by western blotting, quantitative polymerase chain reaction, immunohistochemistry, fluorescence in situ hybridization, fluorescein amidite-tyrosine-valine-alanine-aspartic acid-fluoromethyl ketone (FAM-YVAD-FMK)/Hoechst 33342/propidium iodide (PI) staining, lactate dehydrogenase (LDH) release assays, and cell viability assessments. …”
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Study on the In Vitro and In Vivo Antioxidant Activity and Potential Mechanism of <i>Polygonum viviparum</i> L. by Zhen Yang, Jingyuan Man, Haoyu Liu, Di Wu, Qiangwen Gu, Hongjuan Zhang, Yu Liu, Dan Shao, Baocheng Hao, Shengyi Wang

“…Furthermore, the candidate antioxidant targets of PV were analyzed and screened using a comprehensive network pharmacology method, and their expression were then examined at the mRNA level and protein level. Our results suggest that PV may protect against H₂O₂-induced oxidative damage in macrophages by activating BCL2L1 and inhibiting ESR1, JAK2/STAT3, and MMP2. …”
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Identification and expression analysis of the heat shock proteins Hsp70, Hsp90, and Hsp90b in Litopenaeus vannamei under low-temperature stress by Min Peng, Hui Wang, Shuiping Wen, Zheng Liang, Zheng Huang, Bin Zhang, Tianchong Chen, Qingyun Liu, Qiangyong Li, Yuan Meng, Yuliu Huang, Chunling Yang, Digang Zeng, Manyuan Li, Weilin Zhu, Yongzhen Zhao

“…Downstream genes regulated by hsp90, such as akt, were initially upregulated, but then became downregulated, under low-temperature stress. RNA interference-mediated knockdown of hsp90 affected PI3K/AKT pathway signaling, which downregulated the downstream genes akt and apoptosis-inhibiting bcl-xl, bcl-2, and creb, and upregulated the apoptosis-promoting genes casp1 and casp9. …”
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PD-L1 and IFN-γ modulate Non-Small Cell Lung Cancer (NSCLC) cell plasticity associated to immune checkpoint inhibitor (ICI)-mediated hyperprogressive disease (HPD) by Stefania Angelicola, Francesca Giunchi, Francesca Ruzzi, Mariateresa Frascino, Mary Pitzalis, Laura Scalambra, Maria Sofia Semprini, Olga Maria Pittino, Chiara Cappello, Irene Siracusa, Ilaria Candida Chillico, Martina Di Noia, Cristian Turato, Silvia De Siervi, Francesco Lescai, Teresa Ciavattini, Giulia Lopatriello, Luca Bertoli, Hugo De Jonge, Luisa Iamele, Annalisa Altimari, Elisa Gruppioni, Andrea Ardizzoni, Marzia Rossato, Francesco Gelsomino, Pier-Luigi Lollini, Arianna Palladini

“…The cell lines were phenotypically and molecularly characterized through immunofluorescence, Western Blotting and RNA-Seq analysis. To assess cell plasticity and aggressiveness, cellular growth patterns were evaluated both in vitro and in vivo through 2D and 3D cell growth assays and patient-derived xenografts establishment. …”
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Downregulation of MerTK in circulating T cells of patients with non-proliferative diabetic retinopathy by Shimiao Bu, Shimiao Bu, Jiang-Yue Ling, Jiang-Yue Ling, Xiaojun Wu, Xiaojun Wu, Liting Zhang, Liting Zhang, Xiangyu Shi, Lang Huang, Zheng Zhao, Ying Yang, Zongqin Xiang, Yong U. Liu, Yong U. Liu, Yufeng Liu, Yuehong Zhang, Yuehong Zhang

“…We analyzed peripheral blood mononuclear cells (PBMCs) using RNA sequencing and quantitative PCR (qPCR) to pinpoint differentially expressed genes (DEGs). …”
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Identification of Genetic Markers of <i>APOM</i> and <i>CYP7A1</i> Genes Affecting Milk Production Traits in Chinese Holstein by Yanan Liu, Zijiao Guo, Junqing Ni, Chendong Yang, Bo Han, Yabin Ma, Jianming Li, Guie Jiang, Weijie Zheng, Dongxiao Sun

“…Additionally, with RNAfold Web Server, rs110098953 and rs378530166 changed the mRNA secondary structures of <i>APOM</i> and <i>CYP7A1</i> genes, respectively. …”
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CD38 deficiency leads to a defective short-lived transcriptomic response to chronic graft-versus-host disease induction, involving purinergic signaling-related genes and distinct t... by Mercedes Zubiaur, Laura C. Terrón-Camero, Fernando Gordillo-González, Eduardo Andrés-León, Alicia Barroso-del Jesús, Luz María Canet-Antequera, María M. Pérez Sánchez-Cañete, África Martínez-Blanco, Marilú Domínguez-Pantoja, María Botia-Sánchez, Sonia Pérez-Cabrera, Nerea Bello-Iglesias, Antonio Alcina, Ana-Clara Abadía-Molina, Fuencisla Matesanz, Esther Zumaquero, Ramón Merino, Jaime Sancho

“…This study aimed to elucidate the transcriptomic signatures and dysregulated pathways associated with the autoimmune response in Cd38-/- mice compared to wild-type (WT) mice within the bm12 chronic graft-versus-host disease (cGVHD) lupus model. We conducted bulk RNA sequencing on peritoneal exudate cells (PECs) and spleen cells (SPC) at two and four weeks following adoptive cell transfer. …”
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Eupalinolide B inhibits periodontitis development by targeting ubiquitin conjugating enzyme UBE2D3 by Wenhua Kuang, Ruishen Zhuge, Ping Song, Letai Yi, Shujie Zhang, Ying Zhang, Yin Kwan Wong, Ruixing Chen, Junzhe Zhang, Yuanbo Wang, Dandan Liu, Zipeng Gong, Peili Wang, Xiangying Ouyang, Jigang Wang

“…And this was confirmed by siRNA‐mediated gene knockdown in inflammatory macrophages. …”
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Role of hepatocyte RIPK1 in maintaining liver homeostasis during metabolic challenges by Weigao Zhang, Hu Liu, Danyang Zhang, Yuguo Yi, Liang Tao, Yunfeng Zhu, Shuxian Huang, Xunan Zhao, Qianchao Shao, Peiqi Li, Yiwen Weng, Wei Lu, Jianfa Zhang, Haibing Zhang, Yuxin Chen, Dan Weng

“…Transcriptomic analysis revealed that short-term fasting oriented the hepatic microenvironment into an inflammatory state in Ripk1-hepKO mice, with up-regulated expression of inflammation and immune cell recruitment-associated genes. Single-cell RNA sequencing further confirmed the altered cellular composition in the liver of Ripk1-hepKO mice during fasting, highlighting the increased recruitment of macrophages to the liver. …”
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MiR-216a-3p inhibits the cytotoxicity of primary natural killer cells by Rowan Abdelbary, Rowan Abdelbary, Manon Ragheb, Manon Ragheb, Shereen A. El Sobky, Nagwa El-Badri, Nourhan Aboud, Ahmed Tawheed, Asmaa Gomaa, Mona Zidan, Ramy K. Aziz, Abd Elrahman Abouzid, Radwa Ayman Salah, Mohamed El-Kassas, Imam Waked, Ahmed Moustafa, Ahmed Moustafa, Injie Omar Fawzy, Nada El-Ekiaby, Ahmed Ihab Abdelaziz

“…Thus, we evaluated FOXO-1 expression upon mimicking miR-216a-3p in control NK cells that showed significant downregulation of FOXO-1 on both RNA and protein levels.ConclusionIn conclusion, we report miR-216-3p as a negative regulator of NK cell cytotoxicity.…”
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Longitudinal Survey of Astrovirus infection in different bat species in Zimbabwe: Evidence of high genetic Astrovirus diversity by Chidoti, Vimbiso, De Nys, Hélène, Abdi, Malika, Mashura, Getrude, Pinarello, Valérie, Chiweshe, Ngoni, Matope, Gift, Guerrini, Laure, Pfukenyi, Davies, Cappelle, Julien, Mwandiringana, Ellen, Missé, Dorothée, Gori, Elizabeth, Bourgarel, Mathieu, Liégeois, Florian

“…Astroviruses and bat mitochondrial genes were amplified using pan-AstVs and CytB /12S RNA PCR systems respectively. Phylogenetic analysis of the RdRp Astrovirus sequences revealed a high genetic diversity of astroviruses. …”
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Gut bacteria Prevotellaceae related lithocholic acid metabolism promotes colonic inflammation by Liping Chen, Zhenghao Ye, Junhua Li, Lijia Wang, Yu Chen, Meiping Yu, Jian Han, Jiangeng Huang, Dongyan Li, Yongling Lv, Kai Xiong, De’an Tian, Jiazhi Liao, Ursula Seidler, Fang Xiao

“…The composition of the microbiota in fecal samples from IBD patients and DSS-colitis mice was characterized via Illumina MiSeq sequencing of the bacterial 16S rRNA gene V3-V4 region. Metagenomic profiling further identified metabolism-related gene signatures in stool samples from DSS-colitis mice. …”
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Unveiling the prognostic value of ARID3A in breast cancer through bioinformatic analysis by Wen-Run Cai, Xu-Gang Sun, Yue Yu, Xin Wang, Xu-Chen Cao, Xiao-Feng Liu

“…This study investigates ARID3A's expression, prognostic relevance, clinicopathological correlations, co-expression profiles, and protein-protein interactions in breast cancer. Methods: ARID3A mRNA and protein expression levels were analyzed using UALCAN, GEPIA databases, and immunohistochemistry from our hospital samples. …”
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