The Characteristics Variation of Hepatic Progenitors after TGF-β1-Induced Transition and EGF-Induced Reversion by Ping Wang, Min Cong, Tianhui Liu, Aiting Yang, Guangyong Sun, Dong Zhang, Jian Huang, Shujie Sun, Jia Mao, Hong Ma, Jidong Jia, Hong You

“…The proliferation ability and differentiation potential of these cells were suppressed by TGF-β1, while EGF resumed these capacities to the level similar to the control cells. RNA microarray analysis showed that most of the genes with significant changes after TGF-β1 incubation were recovered by EGF. …”
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Aquaponics model can provide better productivity: Microorganisms affect the growth and health of tilapia by Dingyue Fan, Huimin Xu, Shunlong Meng, Limin Fan, Chao Song, Liping Qiu, Dandan Li, Longxiang Fang, Zhuping Liu, Xuwen Bing

“…To evaluate bacterial community diversity and compositions across different microhabitats in the two systems, we used amplicon-based high-throughput sequencing technique targeting the 16S rRNA gene. In aquaponics, fish had a much greater nitrogen utilization rate, weight gain rate (WGR), feed conversion ratio (FCR), and specific growth rate (SGR) than in controls. …”
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Gain-of-function ANXA11 mutation cause late-onset ALS with aberrant protein aggregation, neuroinflammation and autophagy impairment by Qing Liu, Ye Sun, Baodong He, Haodong Chen, Lijing Wang, Gaojie Wang, Kang Zhang, Ximeng Zhao, Xinzhe Zhang, Dongchao Shen, Xue Zhang, Liying Cui

“…Abstract Mutations in the ANXA11 gene, encoding an RNA-binding protein, have been implicated in the pathogenesis of amyotrophic lateral sclerosis (ALS), but the underlying in vivo mechanisms remain unclear. …”
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Mass cytometry: exploring the immune landscape of systemic autoimmune and inflammatory diseases in the past fourteen years by Aïcha Kante, Aïcha Kante, Mathieu F. Chevalier, Damien Sène, Damien Sène, Jeanne Chauffier, Jeanne Chauffier, Stéphane Mouly, Stéphane Mouly, Benjamin Glenn Chousterman, Benjamin Glenn Chousterman, Fériel Azibani, Benjamin Terrier, Benjamin Terrier, Théo Pezel, Théo Pezel, Cloé Comarmond, Cloé Comarmond

“…We also delve into the bioinformatical approaches applied to mass cytometry to analyze the high volumes of data generated, as well as the impact of the use of complementary single cell RNA sequencing, and their spatial modalities. Our analysis highlights the fact that mass cytometry captures major information on cell populations providing insights on the complex pathogenesis of autoimmune diseases. …”
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高脂饮食诱导<italic>APP_swe/PS1_ΔE9</italic>小鼠脑内炎症反应及细胞焦亡损伤神经功能 by 李泽, 宋祯彦, 李富周, 贺春香, 杨苗, 于文静, 成绍武

“…高脂饮食和<italic>APP/PS1</italic>基因对小胶质细胞激活和IL-1β mRNA表达存在交互作用（<italic>P</italic>&lt;0.05）。痴呆小鼠喂食高脂饲料后，海马区caspase-1/TUNEL表达增强（<italic>P</italic>&lt;0.05），神经细胞焦亡增加，海马C1区神经元尼氏体损伤更严重。…”
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Beta 3 adrenoreceptors protect from hypertrophic remodelling through AMP‐activated protein kinase and autophagy by Emilie Dubois‐Deruy, Roselle Gelinas, Christophe Beauloye, Hrag Esfahani, Lauriane Y.M. Michel, Chantal Dessy, Luc Bertrand, Jean‐Luc Balligand

“…These effects were reproduced by TAC in WT mice but restored to basal levels in β3‐AR expressing cells/mice. siRNA targeting of AMPK partly abrogated the anti‐hypertrophic effect of β3‐AR in response to PE in NRVM. …”
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Berberine alleviates enterotoxigenic Escherichia coli-induced intestinal mucosal barrier function damage in a piglet model by modulation of the intestinal microbiome by Min Du, Xinran Liu, Xu Ji, Yue Wang, Xiaodan Liu, Chunfang Zhao, Chunfang Zhao, Erhui Jin, Erhui Jin, Youfang Gu, Youfang Gu, Hongyu Wang, Feng Zhang, Feng Zhang

“…Furthermore, berberine increased the optical density of mucin 2 and the mucin 2, P-glycoprotein, and CYP3A4 mRNA expression levels (P &lt; 0.05). Berberine increased the expressions of zonula occludins-1 (ZO-1), zonula occludins-2 (ZO-2), Claudin-1, Occludin, and E-cadherin in the ileum (P &lt; 0.05). …”
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Optimization and characterization studies of poultry waste valorization for peptone production using a newly Egyptian Bacillus subtilis strain by Hajar Saeed, Anthony Ragaey, Ziad Samy, Viola Ashraf, Aly ElMostafa, Norhan Ahmad, Enjy Bebawy, Nour ElHoda M. Sorour, Salwa M. El-Sayed, Ashraf Bakry, Naglaa Ebeed, Hesham Elhariry, Thanaa El-Noby, Samah H. Abu-Hussien

“…The isolation process yielded the highly potent proteolytic B.subtilis isolate P6, identified through 16S rRNA gene sequencing to share 94% similarity with the B.subtilis strain KEMET024 (GenBank accession number PP694485.1) and deposited in MIRCEN culture collection, Cairo, Egypt as EMCC 998871. …”
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A novel truncated fusion gene ETV6::AC010198.2 in post-MPN acute myeloid leukemia caused by del(12)(p13p11) by Jiao Lu, Jiao Lu, Guanqun Yang, Qiurong Zhang, Man Wang, Man Wang, Lijun Wang, Lijun Wang, Lu Chen, Haoyue Chen, Xin Jiang, Chunhua Liu, Tong Lin, Qiaoyan Han, Zefa Liu, Shi Zhen, Zhao Zeng, Jinlan Pan, Jiannong Cen, Suning Chen, Suning Chen, Zheng Wang, Zheng Wang, Xingxia Zhang, Miao Sun

“…Here, we report a long-chain noncoding RNA AC010198.2 as a novel fusion partner of ETV6, showing a karyotype of del(12)(p13p11), with poor prognosis in a post-MPN AML that has never been reported, which may be an vital initial event in the transformation of MPN to AML and deterioration of disease.…”
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Mitochondrial reactive oxygen species trigger metformin-dependent antitumor immunity via activation of Nrf2/mTORC1/p62 axis in tumor-infiltrating CD8T lymphocytes by Kazuhiro Kakimi, Hirokazu Matsushita, Masaaki Komatsu, Eiryo Kawakami, Mikako Nishida, Nahoko Yamashita, Taisaku Ogawa, Keita Koseki, Eiji Warabi, Tomoyuki Ohue, Katsuyuki Shiroguchi, Heiichiro Udono

“…In addition, conditional knockout mice for Nrf2 and p62 were used to detect these markers, together with the monitoring of in vivo tumor growth. RNA sequencing was performed for CD8TILs and tumor cells. …”
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Clinical Characteristics, Laboratory Parameters, and Molecular Epidemiology of Neuroinvasive Flavivirus Infections in a Hotspot Region of Eastern Croatia by Dario Sabadi, Kristian Bodulić, Vladimir Savić, Nika Vlahović Vlašić, Maja Bogdanić, Ljiljana Perić, Irena Tabain, Dubravka Lišnjić, Mario Duvnjak, Snježana Židovec-Lepej, Barbara Grubišić, Ilija Rubil, Ljubo Barbić, Luka Švitek, Vladimir Stevanović, Petra Smajić, Bernarda Berišić, Mihaela Zlosa, Ivana Rončević, Tatjana Vilibić-Čavlek

“…Reverse-transcription polymerase chain reaction (RT-qPCR) was used to detect flavivirus RNA in clinical samples (cerebrospinal fluid; CSF, urine). …”
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Fecal microbiota is more stable during degradation and more diverse for ex situ cheetahs in Namibia compared to the USA by Morgan A. Maly, Morgan A. Maly, Morgan A. Maly, Morgan A. Maly, Reade B. Roberts, Mia M. Keady, Anne Schmidt-Küntzel, Meagan Maxwell, Laurie Marker, Matthew Breen, Carly R. Muletz-Wolz, Adrienne E. Crosier

“…All samples were analyzed for bacterial community diversity and composition using 16S rRNA gene amplicon sequencing. First, over a five-day sampling period in Namibia, subsamples 1-3 days post-fresh showed no changes in bacterial diversity or composition compared to fresh subsamples. …”
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Exploring proteomic immunoprofiles: common neurological and immunological pathways in multiple sclerosis and type 1 diabetes mellitus by Fátima Cano-Cano, Almudena Lara-Barea, Álvaro Javier Cruz-Gómez, Francisco Martín-Loro, Laura Gómez-Jaramillo, María Carmen González-Montelongo, María Mar Roca-Rodríguez, Lucía Beltrán-Camacho, Lucía Forero, Javier J. González-Rosa, Mª Carmen Durán-Ruiz, Ana I. Arroba, Manuel Aguilar-Diosdado

“…Predictive analysis highlighted 15 common proteins, up- or downregulated in PBMCs from patients with T1DM and MS patients vs. healthy controls, involved in the immune system activity (BTF3, TTR, CD59, CSTB), diseases of the neuronal system (TTR), signal transduction (STMN1, LAMTOR5), metabolism of nucleotides (RPS21), proteins (TTR, ENAM, CD59, RPS21, SRP9) and RNA (SRSF10, RPS21). In addition, this study revealed both shared and distinct molecular patterns between the two conditions. …”
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Expression profile of Bcl‐2 family proteins in newly diagnosed multiple myeloma patients by Cristina De Ramón, Elizabeta A. Rojas, Irena Misiewicz‐Krzeminska, Ignacio J. Cardona‐Benavides, Myriam Cuadrado, Isabel Isidro, María‐José Calasanz, Manuela Fernandez, Ramón García‐Sanz, Noemi Puig, M. Teresa Cedena, Bruno Paiva, Laura Rosiñol, Joaquín Martínez‐López, Joan Bladé, Juan J. Lahuerta, Jesús F. San Miguel, María V. Mateos, Luis A. Corchete, Norma C. Gutiérrez, GEM/PETHEMA cooperative study group

“…To date, their expression in multiple myeloma (MM) patients has mostly been analyzed at the RNA level. In the present study, we quantified for the first time the protein expression of the Bcl2‐family members using a capillary electrophoresis immunoassay in 120 newly diagnosed MM patients, aged ≤65 years, treated in the context of the PETHEMA/GEM2012 study. …”
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USP14 inhibition by degrasyn induces YAP1 degradation and suppresses the progression of radioresistant esophageal cancer by Fang Yuan, Juan Xu, Lingmei Xuan, Chan Deng, Wei Wang, Rong Yang

“…Methods: To evaluate the expression of USP14 in ESCA tissues or cells, we used RNA-Seq, immunoblotting, co-immunoprecipitation (Co-IP), ubiquitination, quantitative real-time polymerase chain reaction (qRT-PCR), and immunofluorescence assays in this investigation. …”
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Vaccarin suppresses diabetic nephropathy through <?A3B2 pi6?>inhibiting the EGFR/ERK1/2 signaling pathway by Zhu Xuexue, Meng Xinyu, Du Xinyao, Zhao Chenyang, Ma Xinyu, Wen Yuanyuan, Zhang Shijie, Hou Bao, Cai Weiwei, Du Bin, Han Zhijun, Xu Fei, Qiu Liying, Sun Haijian

“…RT-PCR is utilized to quantify the mRNA levels of renal fibrosis, oxidative stress and inflammation markers. …”
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Safety and efficacy of telaprevir in treatment of a chronic hepatitis C in patients of the Russian population included in early access program study by D. T. Abrurakhmanov, V. G. Morozov, I. G. Nikitin, P. O. Bogomolov, N. I. Geyvandova, Ye. N. Bessonova, I. G. Bakulin, V. A. Isakov, K. V. Zhdanov, M. V. Mayevskaya, Ye. A. Nurmukhametova, E. Z. Burnevich, Ye. V. Chesnokov, V. D. Pasechnikov, A. Hill, I. Lonjon-Domanec, S. N. Kizhlo

“…Total of 153 patients have completed 12-week course of triple therapy and 4-week course of peginterferon-alpha and ribavirin treatment (48% cirrhotic patients, 97% – HCV-1b). The level of HCV RNA was undetectable both at the 4-th week, and at the 12-th week (extended rapid virologic response) in 42 (75%) of 56 previously untreated patients, in 34 (89%) of 38 with relapses after previous treatment, in 4 (57%) of 7 with previous incomplete response, in 22 (52%) of 42 with the previous zero response and in 7 (70%) of 10 with previous virologic breakthrough. …”
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Development and characterization of orthotopic patient-derived xenograft models of peritoneal metastatic mucinous appendiceal adenocarcinoma by I. Ito, V.K. Pattalachinti, A.M.G. Yousef, S. Chowdhury, M.M. Fanaeian, E. Haque, B.B. Gunes, M. Yousef, E.R. Salle, M.A. Zeineddine, S. Ji, R. Li, W. Wang, B.A. Helmink, M.W. Taggart, M.G. White, K.F. Fournier, N.W. Fowlkes, J.P. Shen

“…Comparison of histological, immunohistochemical, and molecular analyses including both RNA and DNA sequencing revealed that the PDX models recreate many of the features of metastatic AAs, but also displayed several differences between paired PDX and human tumors, highlighting the intratumoral heterogeneity of AAs within each patient. …”
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Chromosome-level reference genome and annotation of the Arctic fish Anisarchus medius by Ruoyu Liu, Ziyu Meng, Yinan Mu, Ran Zhang, Hanhui Ma, Jingjing Hu, Yanan Wang, Yuxin Shi, Yanan Li, Chaofeng Wang, Weini Zhang, Longshan Lin, Ping Zheng, Xinhua Chen

“…Repetitive sequences accounted for 240.83 Mb (32.58%) of the genome. Non-coding RNA annotations included 4,928 rRNAs, 9,663 tRNAs, 347 snRNAs, and 21 snoRNAs. …”
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HDAC and MEK inhibition synergistically suppresses HOXC6 and enhances PD-1 blockade efficacy in BRAFV600E-mutant microsatellite stable colorectal cancer by Xin Li, Peng Yang, Jin Gu, Nan Chen, Dengbo Ji, Jinhong Xia, Jinying Jia, Zhuang Sun, Mengyuan Shi, Hanyang Wang, Zhaoya Gao

“…Concurrently, we explored the immune characteristics of BRAFV600E MSS CRC using RNA sequencing and multiplex immunohistochemistry. …”
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