Tertiary Lymphoid Structure in Dental Pulp: The Role in Combating Bacterial Infections by Ruiqi Li, Fan Gu, Linlin Peng, Tingting Huan, Zhuo Zhou, Yaling Song, Jinmei He, Kaili Ye, Yao Sun, Tiejun Li, Miao He, Zhuan Bian, Wei Yin

“…In the present study, single‐cell RNA sequencing of healthy and inflamed human dental pulp reveals known markers of TLS, including C‐C motif chemokine ligand 19 (CCL19), lysosome‐associated membrane glycoprotein 3 (LAMP3), CC chemokine receptor 7 (CCR7), and CD86, present in inflamed dental pulp. …”
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Identification and validation of a prognostic signature of drug resistance and mitochondrial energy metabolism-related differentially expressed genes for breast cancer by Tiankai Xu, Chu Chu, Shuyu Xue, Tongchao Jiang, Ying Wang, Wen Xia, Huanxin Lin

“…Furthermore, we examined immune infiltration and tumor mutation burden of the genes within our model and scrutinized divergences in the immune microenvironment between high- and low-risk groups. Small hairpin RNA and lentiviral plasmids were designed for stable transfection of breast cancer cell lines MDA-MB-231 and HCC1806. …”
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Modulation of local immunity by the vaginal microbiome is associated with triggering spontaneous preterm birth by Yijia Liang, Yijia Liang, Yijia Liang, Changying Zhao, Yan Wen, Dashuang Sheng, Tiantian Wei, Tianqi Hu, Junhui Dai, Guoping Zhao, Guoping Zhao, Guoping Zhao, Sijie Yang, Sijie Yang, Qinghua Wang, Lei Zhang, Lei Zhang

“…Additionally, it sought to uncover the microbial composition and functional characteristics of immune states linked to preterm birth, and to evaluate the impact of bacterial interactions on the initiation of preterm birth.MethodsUtilizing 16S rRNA sequencing data and local immune factor expression data from a publicly available longitudinal pregnancy cohort, we conducted immunophenotyping through unsupervised clustering of the immune factors. …”
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Current data on the circulation of the Q fever pathogen in the Republic of Guinea by Ekaterina V. Naidenova, Kirill S. Zakharov, Dmitry A. Agafonov, Mikhail Yu. Kartashov, Aislu M. Senichkina, Erik S. Khalilov, Abdoul B. Ibrahim, Mamadou B. Bah, Ibrahim Nourdine, Nikolai K. Tokarevich, Sanaba Boumbaly, Yusuf Sidime, Svetlana A. Shcherbakova, Vladimir V. Kutyrev

“…For the first time, the complete nucleotide sequence of 16S rRNA of the Q fever pathogen (OQ152497–OQ152500) identified on the territory of Guinea was determined and registered in the GenBank database. …”
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A pan-immune panorama of bacterial pneumonia revealed by a large-scale single-cell transcriptome atlas by Kun Xiao, Yan Cao, Zhihai Han, Yuxiang Zhang, Laurence Don Wai Luu, Liang Chen, Peng Yan, Wei Chen, Jiaxing Wang, Ying Liang, Xin Shi, Xiuli Wang, Fan Wang, Ye Hu, Zhengjun Wen, Yong Chen, Yuwei Yang, Haotian Yu, Lixin Xie, Yi Wang

“…Here, we employed single-cell RNA sequencing of 444,146 bronchoalveolar lavage fluid cells (BALFs) from a large cohort of 74 individuals, including 58 patients with mild (n = 22) and severe (n = 36) diseases as well as 16 healthy donors. …”
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Effect of Chloroform Extraction Site of Jiangu Granule on Obteoblast Differentiation <italic>in Vitro</italic> via BMP2/Smad1/Runx2/Osterix Signaling Pathway by ZHOU Fen, SUN Yuqing, SUN Pan, ZHANG Chutian, YANG Juan, LIN Yanping

“…After cell interventions in each experiment group, RT-PCR was used to detect the mRNA expression levels of BMP2, Smad1, Runx2, Osterix and osteoblast differentiation-related genes ALP and Collagen Ⅰ in each group. …”
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L-glutaminase synthesis by Klebsiella pneumoniae (AS KP 23) isolated from clinical strain, and its efficacy against human hepatocellular and breast cancer cell lines by Lina J. Abdel-Hafez, Eman Y. T. Elariny, Asmaa E. Ibrahim, Mahmoud E. F. Abdel-Haliem

“…Results In this study, a bacterium extracellular from human stool samples was extracted and identified using morphological, biochemical, and molecular methods. The 16 S rRNA gene was 100% identical to the sequence from Klebsiella pneumoniae and was submitted to GenBank under accession number OQ703039. …”
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IGF2BP3 curbed by miR-15c-3p restores disrupted lipid storage and progesterone secretion in chicken granulosa cells under oxidative stress through AKT-Raf1-ERK1/2 signaling pathway by Zhongzhen Lin, Yanrong Gong, Chunlin Yu, Chaowu Yang, Lingqian Yin, Donghao Zhang, Yuan Tang, Feng Xu, Ye Wang, Yiping Liu

“…Therefore, we modeled the processes of oxidative stress and antioxidant in chicken granulosa cells. Small RNA sequencing revealed that miR-15c-3p expression was elevated by oxidative stress induction and attenuated by antioxidant curcumin. …”
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Characteristic alterations of gut microbiota and serum metabolites in patients with chronic tinnitus: a multi-omics analysis by Jiang Wang, Jia-Hui Xiang, Xu-Yuan Peng, Min Liu, Le-Jia Sun, Min Zhang, Li-Yuan Zhang, Zhi-Bin Chen, Zheng-Quan Tang, Lei Cheng

“…To explore the connection between gut microbiota and tinnitus, we conducted 16S rRNA sequencing of fecal microbiota and serum metabolomic analysis in a cohort of 70 patients with tinnitus and 30 healthy volunteers. …”
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Whole blood transcriptome profiles of trypanotolerant and trypanosusceptible cattle highlight a differential modulation of metabolism and immune response during infection by Trypan... by Peylhard, Moana, Berthier, David, Dayo, Guiguigbaza-Kossigan, Chantal, Isabelle, Sylla, Souleymane, Nidelet, Sabine, Dubois, Emeric, Martin, Guillaume, Sempéré, Guilhem, Flori, Laurence, Thévenon, Sophie

“…To this end, whole blood genome-wide transcriptome of three trypanotolerant taurine breeds (N’Dama, Lagune and Baoulé), one susceptible zebu (Zebu Fulani) and one African taurine x zebu admixed breed (Borgou) were profiled by RNA sequencing at four time points, one before and three during infection. …”
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The mechanism of tea tree oil regulating the damage of hydrogen sulfide to spleen and intestine of chicken by Yachao Wang, Yilei Liang, Yan Huang, Wei wang, Xiaoyan Long, Li Jiang, Tingting Cheng, Jinfeng Du, Xuegang Luo

“…Intestinal findings indicated that TTO treatment enhanced villus height, reduced crypt depth, and upregulated the expression of Claudin 1, Occludin, and ZO-1 mRNA in the jejunum (P < 0.05). After TTO treatment, H2S-induced oxidative stress injury and apoptosis protein expression in spleen were improved (P < 0.05). …”
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Shenshuaikang enema restores the intestinal barrier and microbiota-gut-kidney axis balance to alleviate chronic kidney disease via NF-κB pathway by Yan Ye, Yan Ye, Xiaopeng Huang, Xiaopeng Huang, Xiaopeng Huang, Xueying Li, Xueying Li, Fei Gao, Wenzhen Zhong, Anqi Tang, Anqi Tang, Liangbin Zhao, Dengpiao Xie, Naijing Ye, Naijing Ye

“…NF-κB-RE-Luc transgenic mice were used to construct a CKD mouse model, and changes in fluorescence intensity in mice and isolated kidney tissues were detected within 1–6 days using a small animal live imager. Finally, 16S rRNA amplicon sequencing was used to monitor changes in intestinal flora in CKD patients before and after SSKE treatment.ResultsWe found that SSKE improves renal function, attenuates renal fibrosis, reduces inflammatory factor levels, and decreases damage to intestinal and renal structures in adenine-induced CKD rats. …”
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MLL4 regulates postnatal palate growth and midpalatal suture development by Jung-Mi Lee, Hunmin Jung, Bruno de Paula Machado Pasqua, Yungki Park, Qinghuang Tang, Shin Jeon, Shin Jeon, Soo-Kyung Lee, Jae W. Lee, Hyuk-Jae Edward Kwon

“…Whole-mount and histological staining at perinatal stages identified that the midline defects in the Mll4-cKO mice emerged as early as 1 day prior to birth, presenting as a widened midpalatal suture, accompanied by increased cell apoptosis in the suture mesenchyme. Genome-wide mRNA expression analysis of the midpalatal suture tissue revealed that MLL4 is essential for the timely expression of major cartilage development genes, such as Col2a1 and Acan, at birth. …”
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Phenotypic and Complete Reference Whole Genome Sequence Analyses of Two <i>Paenibacillus</i> spp. Isolates from a Gray Wolf (<i>Canis lupus</i>) Gastrointestinal Tract by Jessika L. Bryant, Jennifer McCabe, C. Cristoph Klews, MiCayla Johnson, Ariel N. Atchley, Thomas W. Cousins, Maya Barnard-Davidson, Kristina M. Smith, Mark R. Ackermann, Michael Netherland, Nur A. Hasan, Peter A. Jordan, Evan S. Forsythe, Patrick N. Ball, Bruce S. Seal

“…Two bacteria identified as unique <i>Paenibacillus</i> spp. strains by small ribosomal RNA (16S) gene sequencing were isolated from the gastrointestinal tract of a North American Gray Wolf (<i>Canis lupus</i>). …”
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Construction and iterative redesign of synXVI a 903 kb synthetic Saccharomyces cerevisiae chromosome by Hugh D. Goold, Heinrich Kroukamp, Paige E. Erpf, Yu Zhao, Philip Kelso, Julie Calame, John J. B. Timmins, Elizabeth L. I. Wightman, Kai Peng, Alexander C. Carpenter, Briardo Llorente, Carmen Hawthorne, Samuel Clay, Niël van Wyk, Elizabeth L. Daniel, Fergus Harrison, Felix Meier, Robert D. Willows, Yizhi Cai, Roy S. K. Walker, Xin Xu, Monica I. Espinosa, Giovanni Stracquadanio, Joel S. Bader, Leslie A. Mitchell, Jef D. Boeke, Thomas C. Williams, Ian T. Paulsen, Isak S. Pretorius

“…Abstract The Sc2.0 global consortium to design and construct a synthetic genome based on the Saccharomyces cerevisiae genome commenced in 2006, comprising 16 synthetic chromosomes and a new-to-nature tRNA neochromosome. In this paper we describe assembly and debugging of the 902,994-bp synthetic Saccharomyces cerevisiae chromosome synXVI of the Sc2.0 project. …”
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Comparison of the Accuracy of Four Malaria Diagnostic Methods in a High Transmission Setting in Coastal Cameroon by Marcel N. Moyeh, Innocent M. Ali, Dieudonné L. Njimoh, Akindeh M. Nji, Palmer M. Netongo, Marie S. Evehe, Barbara Atogho-Tiedeu, Stephen M. Ghogomu, Wilfred F. Mbacham

“…Venous blood from 320 participants was used to detect the presence of malaria parasite using SD Bioline™ mRDT and Giemsa stained microscopy or spotted on filter paper for PCR amplification of the 18s rRNA gene of Plasmodium sp following standard procedures. …”
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Defining ortholog-specific UHRF1 inhibition by STELLA for cancer therapy by Wenjing Bai, Jinxin Xu, Wenbin Gu, Danyang Wang, Ying Cui, Weidong Rong, Xiaoan Du, Xiaoxia Li, Cuicui Xia, Qingqing Gan, Guantao He, Huahui Guo, Jinfeng Deng, Yuqiong Wu, Ray-Whay Chiu Yen, Srinivasan Yegnasubramanian, Scott B. Rothbart, Cheng Luo, Linping Wu, Jinsong Liu, Stephen B. Baylin, Xiangqian Kong

“…For translating these findings to cancer therapy, we use a lipid nanoparticle (LNP)-mediated mRNA delivery approach in which the short mSTELLA, but not hSTELLA regions are required to reverse cancer-specific DNA hypermethylation and impair colorectal cancer tumorigenicity.…”
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Radiomics signature for dynamic monitoring of tumor inflamed microenvironment and immunotherapy response prediction by Enriqueta Felip, Paolo Nuciforo, Elena Garralda, Joan Frigola, Ramon Amat, Garazi Serna, Francesco Grussu, Kinga Bernatowicz, Olivia Prior, Marta Ligero, Christina Zatse, Rodrigo Toledo, Manel Escobar, Raquel Perez-Lopez

“…Leveraging contrast-enhanced CT images, we characterized TIME through RNA gene expression analysis, using the T cell-inflamed gene expression signature. …”
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The cancer-associated fibroblasts interact with malignant T cells in mycosis fungoides and promote the disease progression by Yige Zhao, Yong Li, Yong Li, Panpan Wang, Mengyan Zhu, Jiaqi Wang, Bo Xie, Chenyu Tang, Yangyang Ma, Shiwen Wang, Sha Jin, Jinhui Xu, Zhao Li, Xiaoyan Zhang, Liuyu Li, Xiuzu Song, Ping Wang

“…However, the pathogenesis of MF and the role of the tumor microenvironment (TME) remain unclear.MethodsWe performed single-cell RNA sequencing on tumor and adjacent normal tissues and peripheral blood mononuclear cell (PBMC) from patients with advanced MF and healthy control (HC). …”
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NaHS alleviates neuropathic pain in mice by inhibiting IL-17-mediated dopamine (DA) neuron necroptosis in the VTA by Jun Wang, Nan Zhang, Hong-Zheng Liu, Jin-Liang Wang, Yong-Bo Zhang, Dong-Dong Su, Li-Min Zhang, Bao-Dong Li, Hui-Tao Miao, Jun Miao

“…Results: In mice exposed to CCI, there was a significant decrease in dopamine neurons, a reduction in MWT and TWL, decreased expression of tyrosine hydroxylase (TH) protein and TH mRNA and an increase in VTA firing rate and MLKL colocalization with DA neurons (all p < 0.05). …”
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