Gut-brain axis mediated by intestinal content microbiota was associated with Zhishi Daozhi decoction on constipation by Leyao Fang, Leyao Fang, Leyao Fang, Xin Yi, Xin Yi, Junxi Shen, Junxi Shen, Na Deng, Na Deng, Xinxin Peng, Xinxin Peng

“…Changes in the gut microbiota were evaluated through 16S rRNA gene sequencing. Additionally, we investigated the correlation between specific microbiota features and the levels of 5-HT, VIP, and AQP3.ResultsThe fecal surface of the mice in the model group (CMM) was rough and dry. …”
Get full text

The Role of circTmeff-1 in Morphine Addiction Memory of Mice by Hailei Yu, Boyang Wen, Yun Lu, Bing Xie, Feng Yu, Minglong Zhang, Chunling Ma, Bin Cong, Di Wen, Haitao Bi

“…Here, we trained mice on a conditional place preference (CPP) model and collected nucleus accumbens (NAc) tissues from day 1 (T0) and day 8 (T1) for high-throughput RNA sequencing. QRT-PCR analysis revealed that circTmeff-1 was highly expressed in the NAc core but not in the NAc shell, suggesting that it plays a role in addiction memory formation. …”
Get full text

Alternations of the gut microbiota and the Firmicutes/Bacteroidetes ratio after biologic treatment in inflammatory bowel disease by Yu-Chieh Tsai, Wei-Chen Tai, Chih-Ming Liang, Cheng-Kun Wu, Ming-Chao Tsai, Wan-Hsiang Hu, Pao-Yuan Huang, Chien-Hung Chen, Yuan-Hung Kuo, Chih-Chien Yao, Seng-Kee Chuah

“…The gut microbial composition was determined by 16 S ribosomal RNA gene sequencing of stool samples. Results: Biologic therapy led to significant clinical improvement in IBD disease activity after 48 weeks. …”
Get full text

High-fat diet promotes gestational diabetes mellitus through modulating gut microbiota and bile acid metabolism by Lei Yao, Xuefei Zhou, Xianqi Jiang, Hao Chen, Yuanliang Li, Xiao Xiong, Yan Tang, Haogang Zhang, Pengfei Qiao

“…Expression levels of Fxr, Shp and Fgf15 mRNA and protein were detected by quantitative reverse transcription polymerase chain reaction and western blot, respectively.ResultsOur data indicated that high fat diet (HFD) was linked with higher prevalence of GDM, and HFD was positively associated with poor prognosis in GDM patients. …”
Get full text

Global vaccine research and application hotspots and trends: a systematic bibliometric analysis based on SCIE highly cited papers by Liming Liang, Xinyu Wang, Yue Zhu, Xu Xia, Runfeng Shi, Huilin Cao, Meixin He, Yuyu Wang, Caizhen You

“…The most highly cited paper was ‘Safety and Efficacy of the BNT162b2 mRNA COVID-19 Vaccine’ from the New England Journal of Medicine, which was cited 9435 times in total. …”
Get full text

Intravesical BCG in patients with non-muscle invasive bladder cancer induces trained immunity and decreases respiratory infections by J Alfred Witjes, Alina Vrieling, Mihai G Netea, Leo A B Joosten, Katja K H Aben, Sita H Vermeulen, Joost L Boormans, Antoine G van der Heijden, Boris Novakovic, Jelmer H van Puffelen, Liesbeth van Emst, Denise Kooper, Tahlita C M Zuiverloon, Ursula T H Oldenhof, Tessel E Galesloot, Lambertus A L M Kiemeney, Egbert Oosterwijk

“…An epigenomics approach combining chromatin immuno precipitation-sequencing and RNA-sequencing with in vitro trained immunity experiments identified enhanced inflammasome activity in BCG-treated individuals. …”
Get full text

Enhancing surveillance for dengue fever in Oyo State, Nigeria – a one health approach by Olawale Sunday Animasaun, Joseph Ojonugwa Shaibu, Busayo Kayode Akomolafe, Olamide Priscilla Animasaun, Piring’ar Mercy Niyang, Olukemi Titilope Olugbade, Akinfemi Oyewumi Akinyode, Ibukun Akinsola Omisakin, Adedapo Olufemi Adeogun, Rosemary Ajuma Audu

“…It involved screening DENV in 289 febrile human blood samples and 1,015 Aedes species mosquitoes. Viral RNA from human and mosquito specimens was extracted and analyzed using RT-qPCR. …”
Get full text

FKBP5 as a key regulator of metabolic processes in birds: Insights from chicken pectoral muscle by Pengfei Du, Xiangli Zhang, Yao Zhu, Ziyang Wang, Xuemeng Si, Huaiyong Zhang, Yanqun Huang, Wen Chen

“…Insulin regulates FKBP5 expression in pectoral muscles in a time-dependent manner, while pyruvate increases FKBP5 levels and fasting/energy restriction reduces FKBP5 mRNA expression in the pectoral muscles.…”
Get full text

Effect of fructo-oligosaccharides on growth performance and meat quality in broilers by ZhiHui Yan, XiaoWu Tang, RunTao Wu, Can Yang, YunMiao Jiang, Xuan Wang, QingHai Tang, YongLing Hu, LeLi Wang, Zhi Jiang

“…There was an increasing trend for total antioxidant capacity (T-AOC) activity of thigh muscle in FOS group compared to CON group (P = 0.053). Relative mRNA expression of breast catalase (CAT), superoxide dismutase 1 (SOD1), thioredoxin reductase 1 (TXNRD) were up-regulated by FOS supplementation compared with CON group (P < 0.05). …”
Get full text

Intrahepatic CD161hiCD8+T Cell Recruitment Has a Pathogenetic Potential in Chronic HBV Infection by Jianfei Li, Qian Liu, Wanlu Duan, Zhi Duan, Futing Liu, Mengqi Ruan, Qiyin Zong, Hao Zhang, Qiang Zhou, Qin Wang

“…Accordingly, the CXCL16 mRNA expression in the liver tissue of CHB‐replication mice was markedly higher than in acute HBV‐replicating and control mice. …”
Get full text

New insight into the role of the pathway NLRP1 and NLRP3 inflammasomes and IL-33 in ultraviolet-induced cutaneous carcinogenesis by Aleksandra Lesiak, Aleksandra Lesiak, Karolina Wodz, Justyna Ceryn, Dorota Sobolewska-Sztychny, Dorota Sobolewska-Sztychny, Igor Bednarski, Janusz Piekarski, Marta Pabianek, Dariusz Nejc, Izabela Dróżdż, Joanna Narbutt, Marcin Noweta, Magdalena Ciążyńska, Magdalena Ciążyńska

“…Silencing NLRP1 or NLRP3 via RNA interference in primary human keratinocytes resulted in a significant reduction of cytokine gene expression. …”
Get full text

二甲双胍和孟鲁司特在急性和慢性过敏性哮喘小鼠模型中的作用 by 郭妍蓉, 严彦

“…各实验组小鼠处死后苏木素-伊红染色和马松染色分析小鼠的肺部病理特征；采用Image J软件进行气道炎症细胞浸润评分和胶原沉积评分，以及分析支气管形态参数，包括支气管壁厚度和支气管平滑肌厚度；采用ELISA方法检测小鼠血清总IgE水平，qRT-PCR检测炎症因子<italic>IL-4</italic>、<italic>IL-5</italic>、<italic>IL-13</italic> mRNA转录水平。结果慢性模型小鼠的血清总IgE水平、气道周围胶原沉积程度、气道平滑肌厚度显著高于急性模型小鼠（<italic>P</italic>＜0.05）。…”
Get full text

Induction of phospholipase A2 group 4C by HCV infection regulates lipid droplet formation by Masahiko Ito, Jie Liu, Masayoshi Fukasawa, Koji Tsutsumi, Yumi Kanegae, Mitsutoshi Setou, Michinori Kohara, Tetsuro Suzuki

“…Among the transcripts associated with phosphatidylcholine biosynthesis, upregulation of PLA2G4C mRNA was most pronounced following HCV infection. …”
Get full text

Field Deployment of a Mobile Biosafety Laboratory Reveals the Co-Circulation of Dengue Viruses Serotype 1 and Serotype 2 in Louga City, Senegal, 2017 by Idrissa Dieng, Maryam Diarra, Moussa Moïse Diagne, Martin Faye, Marie Henriette Dior Ndione, Yamar Ba, Mamadou Diop, El Hadji Ndiaye, Paolo Marinho de Andrade Zanotto, Boly Diop, Mamadou Ndiaye, Abdoulaye Bousso, Ndongo Dia, Mawlouth Diallo, Aliou Barry, Gamou Fall, Cheikh Loucoubar, Amadou Alpha Sall, Ousmane Faye, Oumar Faye

“…Overall, 960 human sera were collected and tested in the field for the presence of viral RNA by real-time RT-PCR. Serotyping, sequencing of complete E gene, and phylogenetic analysis were also performed. …”
Get full text

Identification of Olfactory Receptors Responding to Androstenone and the Key Structure Determinant in Domestic Pig by Peidong Yang, Tingting Luo, Shuqi Yang, Anjing Zhang, Yuan Tang, Li Chen, Jinyong Wang, Yongju Zhao, Zhining Zhong, Xuemin Li, Ziyin Han, Yupei Zhang, Yue Tang, Jideng Ma, Long Jin, Keren Long, Mingzhou Li, Lu Lu

“…To efficiently and accurately screen pig olfactory receptors responding to androstenone and the key structure determinant, we adapted the high-throughput RNA-seq strategy to screen the altered genes upon androstenone treatment in the olfactory epithelium of pigs, yielding 1397 downregulated genes. …”
Get full text

CFTR Expression Analysis for Subtyping of Human Pancreatic Cancer Organoids by Alexander Hennig, Laura Wolf, Beatrix Jahnke, Heike Polster, Therese Seidlitz, Kristin Werner, Daniela E. Aust, Jochen Hampe, Marius Distler, Jürgen Weitz, Daniel E. Stange, Thilo Welsch

“…Human PDAC organoids can be classified into known subtypes based on KRT81 and CFTR immunoreactivity. CFTR and HNF1A mRNA levels correlated well. Furthermore, subtype-specific immunoreactivity matched well between PDAC organoids and the respective primary tumor tissue. …”
Get full text

Apigenin inhibits NLRP3 inflammasome activation in monocytes and macrophages independently of CD38 by Knut Husø Lauritzen, Knut Husø Lauritzen, Kuan Yang, Michael Frisk, Mieke C. Louwe, Maria Belland Olsen, Maria Belland Olsen, Mathias Ziegler, William E. Louch, Bente Halvorsen, Bente Halvorsen, Pål Aukrust, Pål Aukrust, Arne Yndestad, Øystein Sandanger, Øystein Sandanger

“…IL-1β and TNF secretion and mRNA expression, as well as N-terminal gasdermin-D formation were quantified. …”
Get full text

Permafrost pore structure and its influence on microbial diversity: Insights from X-ray computed tomography by Nathan D. Blais, Joy M. O’Brien, Hannah Holland-Moritz, Lauren Farnsworth, Robyn A. Barbato, Thomas A. Douglas, Alexandra Contosta, Julie Bobyock, Erin C. Rooney, Taylor Sullivan, Jessica Gilman Ernakovich

“…The Euler characteristic number was used to measure pore connectivity and serve as a proxy for potential hydrological connectivity, as direct measurement of hydrological connectivity was not possible. DNA and RNA were extracted from the scanned permafrost and analyzed via amplicon sequencing of the 16S region to quantify the total and active microbial community diversity. …”
Get full text

Full-length transcriptome analysis of male and female gonads in Japanese Eel (Anguilla japonica) by Jiangling Li, Jingjie Liang, Mengyang Wang, Yuewen Jiang, Wen Li, Mingxi Huang, Yan Huang, Yangjie Xie, Jianchun Chen, Tiansheng Chen

“…Results PacBio Iso-Seq and Illumina RNA-Seq technologies were combined to conduct a full-length transcriptome analysis of male and female Japanese eel gonads at a post-differentiation, pre-maturation stage. …”
Get full text

Wu-Mei-Wan enhances brown adipose tissue function and white adipose browning in obese mice via upregulation of HSF1 by Shen Chen, Kexin Nie, Hongzhan Wang, Yang Gao, Xinyue Jiang, Hao Su, Zhi Wang, Yueheng Tang, Fuer Lu, Hui Dong, Jingbin Li

“…Protein expression was assessed using western blot, immunohistochemistry, immunofluorescence and RT-qPCR were employed to detect the mRNA levels. Statistical analyses included t-tests, ANOVA, and non-parametric tests like the Mann–Whitney U test or Kruskal–Wallis test. …”
Get full text