Leupaxin promotes hepatic gluconeogenesis and glucose metabolism by coactivation with hepatic nuclear factor 4α by Xiaomin Luo, Fang Liu, Lijun Zhu, Caizhi Liu, Ruhui Shen, Xiaoyin Ding, Yufan Wang, Xiaofang Tang, Yongde Peng, Zhijian Zhang

“…We further leveraged cell cultures and mouse models to validate the functional importance of molecular pathway during gluconeogenesis by using adenovirus-mediated overexpression and adeno-associated virus shRNA–mediated knockdown both in vivo and ex vivo, such as in ob/db/DIO mice, HepG2 and primary hepatocytes. …”
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Real-world of Limosilactobacillus reuteri in mitigation of acute experimental colitis by Ningning Yue, Hailan Zhao, Peng Hu, Yuan Zhang, Chengmei Tian, Chen Kong, Zhiliang Mai, Longbin Huang, Qianjun Luo, Daoru Wei, Ruiyue Shi, Shaohui Tang, Yuqiang Nie, Yujie Liang, Jun Yao, Lisheng Wang, Defeng Li

“…Second, L. reuteri-CMVs were taken up by intestinal epithelial cells (IECs), elevated the expression of ZO-1, E-cadherin (Cdh1), and Occludin (Ocln), decreased intestinal permeability, and exerted protective effects on epithelial tight junction functionality. RNA sequencing analysis demonstrated that L. reuteri-CMVs repaired intestinal barrier by activating the HIF-1 signaling pathway and upregulating HMOX1 expression. …”
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Exploring the relationship between sepsis and Golgi apparatus dysfunction: bioinformatics insights and diagnostic marker discovery by Wanli Ma, Xinyi Liu, Ran Yu, Jiannan Song, Lina Hou, Ying Guo, Hongwei Wu, Dandan Feng, Qi Zhou, Haibo Li

“…This study aims to explore the relationship between sepsis and the Golgi apparatus using bioinformatics, offering fresh insights into its diagnosis and treatment.MethodsTo explore the role of Golgi-related genes in sepsis, we analyzed mRNA expression profiles from the NCBI GEO database. …”
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Comparative impact of tertiary lymphoid structures and tumor-infiltrating lymphocytes in cholangiocarcinoma by Chun-Nan Yeh, Yu-Chan Chang, Yi-Chen Yeh, Yu-Chao Wang, Dennis Shin-Shian Hsu, Meng-Lun Lu, Michael Hsiao, Ming-Huang Chen, Nai-Jung Chiang, Shin-Yi Chung, Chien-Jung Huang, Ming-Hsien Chan, Tzu-Sheng Hsu, Yi-Ping Hung

“…Immunohistochemistry and RNA sequencing were employed to assess TLSs and TILs presence and activity. …”
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Understanding the mechanisms of hypoxia-induced tissue damage in fish: The role of GasderminEa/b in Larimichthys crocea by Lin Wang, Shuaiyu Chen, Yongyang Wang, Weini Zhang, Yinnan Mu, Yudong Jia, Changtao Guan, Meng Xue, Xinhua Chen

“…Compared to LcGSDMEb, the expression of LcGSDMEa was higher in both mRNA and protein levels, thus exhibiting a stronger response during hypoxia stress. …”
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Uridine-cytidine kinase 2 is correlated with immune, DNA damage repair and promotion of cancer stemness in pan-cancer by Jinlong Tian, Yanlei Li, Yu Tong, Yuan Zhang, Tingxiao Zhao, Yao Kang, Qing Bi

“…BackgroundUCK2 (Uridine-Cytidine Kinase 2) is a promising prognostic marker for malignant tumors, but its association with immune infiltration and cancer stemness in pan-cancer remains to be fully understood. we find that gene UCK2 is closed related to RNA stemness scores (RNAss) and DNA stemness scores (DNAss), which is measured the tumor stemness. …”
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3D bioprinting of engineered exosomes secreted from M2-polarized macrophages through immunomodulatory biomaterial promotes in vivo wound healing and angiogenesis by Sayan Deb Dutta, Jeong Man An, Jin Hexiu, Aayushi Randhawa, Keya Ganguly, Tejal V. Patil, Thavasyappan Thambi, Jangho Kim, Yong-kyu Lee, Ki-Taek Lim

“…Moreover, the COL@d-ECM/M2-Exo was found to be biocompatible with skin cells. Transcriptomic (RNA-Seq) and real-time PCR (qRT-PCR) study revealed that co-culture of fibroblast/keratinocyte/stem cells/endothelial cells in a 3D bioprinted COL@d-ECM/M2-Exo hydrogel upregulated the skin-associated signature biomarkers through various regulatory pathways during epidermis remodeling and downregulated the mitogen-activated protein kinase (MAPK) signaling pathway after 7 days. …”
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Host-Induced Gene Silencing of the <i>Aspergillus flavus O</i>-Methyl Transferase Gene Enhanced Maize Aflatoxin Resistance by Olanike Omolehin, Yenjit Raruang, Dongfang Hu, Zhu-Qiang Han, Surassawadee Promyou, Robert L. Brown, Qijian Wei, Kanniah Rajasekaran, Jeffrey W. Cary, Kan Wang, Dan Jeffers, Zhi-Yuan Chen

“…Maize is one of the major crops that are susceptible to <i>Aspergillus flavus</i> infection and subsequent aflatoxin contamination, which poses a serious health threat to humans and domestic animals. Here, an RNA interference (RNAi) approach called Host-Induced Gene Silencing (HIGS) was employed to suppress the <i>O</i>-methyl transferase gene (<i>omtA</i>, also called <i>aflP</i>), a key gene involved in aflatoxin biosynthesis. …”
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DRG2 levels in prostate cancer cell lines predict response to PARP inhibitor during docetaxel treatment by Jeong Min Lee, Won Hyeok Lee, Seung Hyeon Cho, Jeong Woo Park, Hyuk Nam Kwon, Ji Hye Kim, Sang Hun Lee, Ji Hyung Yoon, Sungchan Park, Seong Cheol Kim

“…Cells were transfected with DRG2 siRNA, and pcDNA6/V5-DRG2 was used to overexpress DRG2. …”
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Airway microbiota dysbiosis and metabolic disorder in ozone and PM2.5 co-exposure induced lung inflammatory injury in mice by Lin Yang, Fei Xu, Shuaiqi Zhao, Yuling Zeng, Qiong Wu, Ling Zhang, Saige Shi, Fengquan Zhang, Juan Li, Zhen An, Huijun Li, Hui Wu, Jie Song, Weidong Wu

“…After last exposure, bronchoalveolar lavage fluids (BALF) were collected for inflammatory biomarker measurement, 16S rRNA sequencing and metabolite profiling. Lung tissues were processed for histological examination. …”
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Regulation of Shade Avoidance Under Low‐Blue‐Light by MTA in Soybean by Liya Zhang, Jun Liu, Jiaqi Chen, Yanyan Zhang, Chao Qin, Xiangguang Lyu, Zhuang Li, Ronghuan Ji, Bin Liu, Hongyu Li, Tao Zhao

“…Abstract Under low blue light (LBL) conditions, soybean exhibits classic shade avoidance syndrome (SAS) with exaggerated stem elongation (ESE), leading to lodging and yield reduction in dense farming. Recently, mRNA modification by N6‐methyladenosine (m6A) has emerged as a crucial epigenetic mechanism regulating plant biological processes; however, its impact on shade avoidance remains unexplored. …”
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Establishment of <i>Echinococcus granulosus EgM123</i> Recombinant Gene Rabies Virus SRV₉ and Identification of Its Biological Characteristics by Yueqi Yang, Mengdan Hou, Guicheng Su, Xiaoyan Ma, Xiaohui Su, Kunlei Li, Songhan Liu, Luheng Xiao, Jingjing Yao, Jiahao Zhai, Xiaoying Wei, Yang Zhou, Qianqian Lai, Yuwei Dong, Jieyu Liu, Shaohua Zhai

“…The rescued virus-infected BSR cells were characterized by TEM, and the results indicated that bullet-like viral particles with an average size of 148.47 nm and a cyst structure were present in the cytoplasm of BSR cells; the expression levels of continuously cultivated 9th-, 10th-, 11th-, 12th-, and 13th-generation viruses were quantified by qRT-PCR, and the results showed that mRNA expression of the virus was upregulated. The LD₅₀ titer of suckling rats was measured to be 10^−1.4. …”
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The emergence of DNAM-1 as the facilitator of NK cell-mediated killing in ovarian cancer by Rachel Pounds, Rachel Pounds, Wayne Croft, Hayden Pearce, Tasnia Hossain, Kavita Singh, Janos Balega, David N. Jeevan, Sudha Sundar, Sudha Sundar, Sean Kehoe, Jason Yap, Jason Yap, Paul Moss, Jianmin Zuo

“…In contrast, NK cell numbers in primary tumour and metastasis were substantially reduced, with downregulation of activatory receptors together with elevated PD-1 expression. scRNA-Seq identified 5 NK cell subpopulations along with increased exhausted and immature NK cells within tumour tissue compared to normal tissue. …”
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The multi-omics signatures of telomere length in childhood by Congrong Wang, Dries S. Martens, Mariona Bustamante, Rossella Alfano, Michelle Plusquin, Lea Maitre, John Wright, Rosemary R. C. McEachan, Johanna Lepeule, Remy Slama, Marina Vafeiadi, Leda Chatzi, Regina Grazuleviciene, Kristine B. Gutzkow, Hector Keun, Eva Borràs, Eduard Sabidó, Angel Carracedo, Georgia Escarami, Augusto Anguita-Ruiz, Dolors Pelegrí-Sisó, Juan R. Gonzalez, Martine Vrijheid, Tim S. Nawrot

“…Blood DNA methylation, gene expression, miRNA expression, plasma proteins and serum and urinary metabolites were measured through microarrays or (semi-) targeted assays. …”
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Metasilicate-based alkaline mineral water improves the growth performance of weaned piglets by maintaining gut-liver axis homeostasis through microbiota-mediated secondary bile aci... by Jian Chen, Kanwar K. Malhi, Xiaowei Li, Xiangwen Xu, Jianxun Kang, Bichen Zhao, Yaru Xu, Xuenan Li, Jinlong Li

“…To analyze the composition of the gut microbiota, 16S rRNA PacBio SMRT Gene Full-Length Sequencing was performed. …”
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Deficiency of FABP7 Triggers Premature Neural Differentiation in Idiopathic Normocephalic Autism Organoids by Xiao Han, Yuanlin He, Yuanhao Wang, Wenzhu Hu, Chu Chu, Lei Huang, Yuan Hong, Lu Han, Xu Zhang, Yao Gao, Yuan Lin, Hongxia Ma, Hongbing Shen, Xiaoyan Ke, Yan Liu, Zhibin Hu

“…Multiple cell lines and time series scRNA‐seq combined with a histomorphological analysis revealed premature neural differentiation of neural stem cells (NSCs) and decreased expression of Fatty acid binding protein 7 (FABP7) in ASD organoids. …”
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Anti-PL-7/PL-12 antisynthetase syndrome associated with interstitial lung disease following SARS-COV-2 infection and vaccination: A case study review by Laura García-Bravo, Ángela Villegas, Bárbara López Uceda, Anaís Mariscal, Cristina Vadillo, M Asunción Nieto Barbero, Juan Luis Rodríguez-Hermosa, Beatriz Mediero Valeros, José Carlos Plaza-Hernández, Miguel Fernández-Arquero, María Guzmán-Fulgencio, Gloria Candelas-Rodríguez, Silvia Sánchez-Ramón, Juliana Ochoa-Grullón

“…Anti-synthetase syndrome (ASS), a subset of IIM, is defined by the presence of autoantibodies against aminoacyl-tRNA synthetase (anti-ARS) and is strongly associated with interstitial lung disease (ILD), a major contributor to severe complications and reduced survival. …”
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Whole-Genome Methylation Sequencing Analysis and Functional Verification of LIM-Homeobox Family Genes in Cervical Cancer by Yu R, Yu Q, Shi J, Meng X, Deng Z, Suo J, Yang H

“…After 5-Aza-DC and radiotherapy, the methylation of the CpG islands in LHX2, LHX5 and LHX9 genes in these patients was decreased (p < 0.01), and the mRNA and protein expression levels were relatively increased (p < 0.01).Conclusion: In our present work, genome-wide DNA methylation maps of cervical cancer tissues before and after radiotherapy were successfully constructed. …”
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Aberrant Expression of miR-362 Promotes Lung Cancer Metastasis through Downregulation of Sema3A by Dan Luo, Zheng Zhang, Zhao Zhang, Jia-Yue Li, Jian Cui, Wen-Pu Shi, Xi-Wen Dong, Lin Yuan, Peng Lin, Zhi-Nan Chen, Hui-Jie Bian, Zi-Ling Wang

“…miR-362 is a recently discovered member of the microRNA family, and it modulates a variety of physical activities and plays an important role in the occurrence and development of many tumors. …”
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Impact of hepatic steatosis on the efficacy of antiviral treatment for chronic hepatitis B and the establishment of predictive model: a cohort study by Guanghui Ren, Kaining Jia, Shi Yin, Yunpeng Guan, Qingwei Cong, Ying Zhu

“…Results At weeks 24 and 48, the CHB + MASLD group displayed a higher HBsAg response rate than the CHB group (24 weeks: 11.5% vs. 3.8%, p = 0.026; 48 weeks: 24.4% vs. 8.4%, p = 0.001). The pgRNA response was also higher in the CHB + MASLD group at both time points (24 weeks: 30.9% vs. 19.7%, p = 0.163; 48 weeks: 48.8% vs. 28.3%, p = 0.049). …”
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