Therapeutic effect of low-dose BMSCs-Loaded 3D microscaffold on early osteonecrosis of the femoral head by Minzheng Guo, Baochuang Qi, Zijie Pei, Haonan Ni, Junxiao Ren, Huan Luo, Hongxin Shi, Chen Meng, Yang Yu, Zhifang Tang, Yongqing Xu, Qingyun Xue, Chuan Li

“…To understand the mechanism of efficacy, we established a co-culture model of human osteoblasts and BMSCs, followed by cell proliferation and activity detection, flow cytometry analysis, Quantitative RT-PCR, and Western blotting. Additionally, RNA sequencing was performed on femoral head tissues. …”
Get full text

Cecum microbiota composition, fermentation characteristics, and immunometabolic biomarkers of Yunshang black goat fed varying dietary energy and protein levels by Binlong Fu, Xiaoqi Zhao, Muhammad Khan, Yanting Jiang, Weijuan Li, Maida Mushtaq, Baiji Danzeng, Xiaojun Ni, Zobia Azeem, Qingyong Shao, Bai Xue, Yina Ouyang

“…VFA metabolism, absorption, cytokine expression, and tight junction protein mRNA in cecal tissue were also analyzed. Genes like MCT-1 and SLC16A4, linked to VFA absorption, positively correlated with Paludibacter, which was associated with immune markers (TLR-3, TLR-4, IFN-γ) and Occludin expression. …”
Get full text

Differential impact of TIM-3 ligands on NK cell function by Xin Zhang, Lazar Vujanovic, Riyue Bao, Robert L Ferris, Tullia C Bruno, Aditi Kulkarni, Hridesh Banerjee, Housaiyin Li, Juncheng Wang, Lawrence P Kane, Pragati Upadhyay, Jennifer L Anderson, Onyedikachi Victor Onyekachi, Lidia M R B Arantes

“…The primary objectives of this study were to assess the prevalence and function of TIM-3+ natural killer (NK) cells in patients with head and neck squamous cell carcinoma (HNSCC), determine whether the four TIM-3 ligands differentially affect TIM-3+ NK cell functions, identify the most immunosuppressive ligand, and evaluate whether targeting ligand-mediated TIM-3 signaling enhances NK cell effector functions.Methods Single-cell RNA sequencing and flow cytometry were used to study the prevalence, phenotypes and function of TIM-3+ NK cells in HNSCC patient tumors and blood. …”
Get full text

P-glycoprotein-independent cytotoxic effects of 5-aminopyrazole in L1210 leukemia cells by Lucia Sofrankova, Jana Spaldova, Pavol Stefik, Branislav Pavilek, Dusan Bortnak, Lucia Pavlikova, Ivana Zidekova, Daniel Vegh, Viktor Milata, Albert Breier, Zdena Sulova

“…All derivatives, except A5, suppressed P-gp activity, though they did not alter P-gp expression at the mRNA or protein level. Cell cycle analysis showed changes in the G0/G1 and S phases. …”
Get full text

Modelling the potential clinical and economic impact of universal antenatal hepatitis C (HCV) screening and providing treatment for pregnant women with HCV and their infants in Egy... by Giuseppe Indolfi, Diana M Gibb, Aya Mostafa, Sarah Pett, Yazdan Yazdanpanah, Sylvie Deuffic-Burban, Anthony E Ades, Karen Scott, Ali Judd, Nadia Hachicha-Maalej, Clotilde Lepers, Intira Jeannie Collins, Manal H El Sayed

“…Prophylactic treatment at birth for infants born to HCV RNA-positive mothers would also be similarly cost saving, even with treatment uptake as low as 15% (US$218.6, 46.3525 and 0.0359 years). …”
Get full text

<i>Pseudomonas syringae</i> Pathovar <i>syringae</i> Infection Reveals Different Defense Mechanisms in Two Sweet Cherry Cultivars by Claudia Carreras, Alan Zamorano, Luis Villalobos-González, Paula Pimentel, Lorena Pizarro, María Francisca Beltrán, Weier Cui, Manuel Pinto, Franco Figueroa, Carlos Rubilar-Hernández, Analia Llanes, Assunta Bertaccini, Nicola Fiore

“…Cultivars exhibit diverse susceptibility in the field and the molecular mechanisms underlying the differential responses remain unclear. RNA-seq analysis was performed to characterize the transcriptomic response in cultivars Santina and Bing (less and more susceptible to <i>P. syringae</i> pv. …”
Get full text

Genome-wide identification of the Sec14 gene family and the response to salt and drought stress in soybean (Glycine max) by Jinyu Zhang, Liying Zou, Li Wang, Dongchao Zhang, Ao Shen, Yongqi Lei, Maoni Chao, Xinjuan Xu, Zhiwei Xue, Zhongwen Huang

“…Furthermore, the protein interaction network and miRNA regulation associated with GmSec14s were predicted to elucidate the potential functions of GmSec14 members. …”
Get full text

Identification of a 5-Gene-Based Scoring System by WGCNA and LASSO to Predict Prognosis for Rectal Cancer Patients by He Huang, Shilei Xu, Aidong Chen, Fen Li, Jiezhong Wu, Xusheng Tu, Kunpeng Hu

“…Receiver operating characteristic (ROC) curve analysis demonstrated that this risk score had high predictive accuracy for unfavorable prognosis, with the area under the ROC curve of 0.915 and 0.827 for TCGA and GSE56699 datasets, respectively. This five-mRNA classifier was an independent prognostic factor. …”
Get full text

Molecular characterization and lineage analysis of canine astrovirus strains from dogs with gastrointestinal disease in Ecuador based on ORF-2 gene analysis by Anthony Loor-Giler, Anthony Loor-Giler, Silvana Santander-Parra, Sara Castillo-Reyes, Martín Campos, Martín Campos, Renan Mena-Perez, Renan Mena-Perez, Santiago Prado-Chiriboga, Santiago Prado-Chiriboga, Luis Nunez, Luis Nunez

“…Canine Astrovirus (CaAstV) part of the Astroviridae family and genus Mamastrovirus, is a linear RNA virus with a genome of approximately 6.6 kb with three open reading frames (ORF): ORF1a and ORF1b, which code for the most conserved non-structural proteins, and ORF2, which code for the capsid protein, the most variable region of the genome. …”
Get full text

Prevalence of Antibiotic Resistance Genes in Differently Processed Smoothies and Fresh Produce from Austria by Sonia Galazka, Valerie Vigl, Melanie Kuffner, Irina Dielacher, Kathrin Spettel, Richard Kriz, Norbert Kreuzinger, Julia Vierheilig, Markus Woegerbauer

“…The bacterial microbiome, potential pathogens, bacterial ARG carriers and competent bacteria able to take up ARGs were identified by Illumina 16S rRNA gene sequencing. To reduce the risk of AMR spread from smoothies, our data endorse the application of DNA-disintegrating processing techniques such as HPP.…”
Get full text

Effects of Chinese Herbal Medicines on Growth Performance, Antioxidant Capacity, and Liver and Intestinal Health of Hybrid Snakehead (<i>Channa maculata</i> ♀ × <i>Channa. argus</i... by Jiamin Kang, Shuzhan Fei, Junhao Zhang, Haiyang Liu, Qing Luo, Mi Ou, Langjun Cui, Tao Li, Jian Zhao

“…Compared to the control group, the activity of superoxide dismutase (SOD) in plasma and intestine was significantly increased in the FSE and CHMM groups, whereas the content of malondialdehyde (MDA) in plasma and liver was significantly reduced in the SCE group. A 16s rRNA analysis indicates that dietary supplementation with FSE significantly promoted the proliferation of Fusobacteriota, while IRE supplementation increased the alpha diversity of intestinal bacteria. …”
Get full text

Increased Chemokine Production is a Hallmark of Rhesus Macaque Natural Killer Cells Mediating Robust Anti-HIV Envelope-Specific Antibody-Dependent Cell-Mediated Cytotoxicity by Junsuke Nohara, Tyler Evangelous, Madison Berry, Whitney Beck, Sarah Mudrak, Shalini Jha, R. Keith Reeves, Kevin J. Wiehe, Justin Pollara, Georgia Tomaras, Todd Bradley, Guido Ferrari

“…CD107a+ and CD107a– populations were analyzed individually using single-cell RNA sequencing.  Results: Subsets of CD107a+ RMNK cells produced more chemokines than the others, suggesting that these cells not only eliminate infected cells but also provide immunoregulatory signals and potentially curb HIV-1 replication. …”
Get full text

Risk Factors and Circulating Subtypes of <i>Cryptosporidium</i> spp. and <i>Giardia duodenalis</i> in Hospitalized Children in Mozambique by Ofélia Nhambirre, Maria Luísa Lobo, Idalécia Cossa-Moiane, Adilson Bauhofer, Nilsa de Deus, Olga Matos

“…In this study, two nested PCRs targeting <i>ssurRNA</i> and <i>gp60</i> genes were used for genetic diversity of <i>Cryptosporidium</i> spp. and <i>b-giardin</i> for <i>G. duodenalis</i>. …”
Get full text

Ganoderma lucidum spore oil (GLSO), a novel antioxidant, extends the average life span in Drosophila melanogaster by Yi Zhang, Hongfei Cai, Zhu Tao, Cheng Yuan, Zhaojian Jiang, Juyan Liu, Hiroshi Kurihara, Wendong Xu

“…Further, we found dose-dependent increases in the mRNA expression of Cu, Zn-SOD, Mn-SOD, and CAT in GLSO-treated groups. …”
Get full text

Upregulated astrocyte HDAC7 induces Alzheimer-like tau pathologies via deacetylating transcription factor-EB and inhibiting lysosome biogenesis by Jinwang Ye, Suyue Zhong, Huali Wan, Xing Guo, Xuanbao Yao, Qiong Liu, Liming Chen, Jian-Zhi Wang, Shifeng Xiao

“…PS19 mice were treated with AAVs expressing shRNA for HDAC7 with astrocyte-specific promoter and with a selective class IIa HDAC inhibitor, TMP195, and the effects on tau pathology, gliosis, synaptic plasticity and cognition-related behavioral performance were measured. …”
Get full text

Stochastic demethylation and redundant epigenetic suppressive mechanisms generate highly heterogeneous responses to pharmacological DNA methyltransferase inhibition by Mie K. Jakobsen, Sofie Traynor, Aaraby Y. Nielsen, Christina Dahl, Mette Staehr, Simon T. Jakobsen, Maria S. Madsen, Rasmus Siersbaek, Mikkel G. Terp, Josefine B. Jensen, Christina B. Pedersen, Anup Shrestha, Jonathan R. Brewer, Pascal H. G. Duijf, Odd L. Gammelgaard, Henrik J. Ditzel, Alexei F. Kirkin, Per Guldberg, Morten F. Gjerstorff

“…The analysis was performed on breast cancer patient-derived xenograft tumors and cell lines, employing a comprehensive set of techniques, including targeted single-cell mRNA sequencing. Mechanistic insights were further gained through DNA methylation profiling and chromatin structure analysis. …”
Get full text

Pseudovibriamides from Pseudovibrio marine sponge bacteria promote flagellar motility via transcriptional modulation by Yitao Dai, Vitor Lourenzon, Laura P. Ióca, Dua Al-Smadi, Lydia Arnold, Ian McIntire, Roberto G. S. Berlinck, Alessandra S. Eustáquio

“…Here, we define the borders of the pseudovibriamides gene cluster, assign function to biosynthetic genes using reverse genetics, and test the hypothesis that pseudovibriamides impact motility by modulating gene transcription. RNA-sequencing transcriptomic analyses of strains having different compositions of pseudovibriamides suggested that both pseudovibriamides A and B affect genes potentially involved in motility, and that a compensatory mechanism is at play in mutants that produce only pseudovibriamide A, resulting in comparable flagellar motility as the wild type. …”
Get full text

Lymphoma patients treated with anti-CD20 and chemotherapy display disconnected T and B cell responses to COVID-19 vaccine by Sylvain Lamure, Sylvain Lamure, Houria Hendel Chavez, Marie-Ghislaine de Goër de Herve, Luc-Matthieu Fornecker, Bernard Drenou, Caroline Jacquet, Fatiha Merabet, Milena Kohn, Florence Quélin, Angela Jackson, Sylvain Choquet, Rémy Duléry, Yassine Taoufik, Caroline Besson, Caroline Besson

“…We prospectively evaluated CD4, CD8 T-cell and serological responses to the COVID-19 mRNA vaccine in a cohort of patients treated for a B-cell lymphoma with anti-CD20 therapy. …”
Get full text

Host–virus interactions during infection with a wild-type ILTV strain or a glycoprotein G deletion mutant ILTV vaccine strain in an ex vivo system by Gayathri Gopakumar, Mauricio J.C. Coppo, Andrés Diaz-Méndez, Carol A. Hartley, Joanne M. Devlin

“…In the current study, transcriptional profiles of chicken tracheal organ cultures (TOCs), 24 h post inoculation with ∆gG-ILTV or the gG-expressing parent wild-type strain, CSW-1 ILTV were explored and compared with the mock-infected TOCs using RNA-seq analysis. Transcriptomes of the vaccine and wild-type ILTV were also compared with each other. …”
Get full text

Inhibition of MEK1 Signaling Pathway in the Liver Ameliorates Insulin Resistance by Atsunori Ueyama, Nobuhiro Ban, Masanori Fukazawa, Tohru Hirayama, Minako Takeda, Tatsuo Yata, Hiroyasu Muramatsu, Masaki Hoshino, Marii Yamamoto, Masao Matsuo, Yuka Kawashima, Tatsuhiko Iwase, Takehisa Kitazawa, Youichi Kushima, Yuichiro Yamada, Yoshiki Kawabe

“…By means of adenovirus-mediated Mek1 shRNA expression, we confirmed that blood glucose levels are reduced by suppression of MEK1 expression in the liver of db/db mice. …”
Get full text