Protective Effects of Thalidomide on High-Glucose-Induced Podocyte Injury through In Vitro Modulation of Macrophage M1/M2 Differentiation by Hui Liao, Yuanping Li, Xilan Zhang, Xiaoyun Zhao, Dan Zheng, Dayue Shen, Rongshan Li

“…The present study explored the effects of Tha on mRNA and protein expressions of inducible nitric oxide synthase (iNOS), tumor necrosis factor- (TNF-) α, mannose receptor (CD206), and arginase- (Arg-) 1 in HG-activated macrophages. iNOS and TNF-α are established as markers of classically activated macrophage (M1). …”
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RBMS3表达与上皮性卵巢癌临床病理特征及预后 by 邢瑞丽, 印天, 范方田, 张颖, 张庆松, 杨波

“…目的探讨RNA结合基序单链相互作用蛋白3（RBMS3）在上皮性卵巢癌（EOC）组织中的表达，及其与EOC组织的临床病理特征和预后的关系。…”
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Overexpression of miR-124 enhances the therapeutic benefit of TMZ treatment in the orthotopic GBM mice model by inhibition of DNA damage repair by Yuchen Wei, Peng Wang, Jianhui Zhao, Xin Fan, Jun Jiang, Xiuli Mu, Yuzhou Wang, Angang Yang, Rui Zhang, Shijie Hu, Zhangyan Guo

“…In the present study, by integrating comprehensive non-coding RNA-seq data from multiple cohorts of GBM patients, we identified that a series of miRNAs are frequently downregulated in GBM patients compared with the control samples. …”
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Microbial phenolic metabolites 3-(3′,4′-dihydroxyphenyl)propanoic acid and 3′,4′-dihydroxyphenylacetic acid prevent obesity in mice fed with high-fat diet by Wanbing Chen, Ruonan Liu, Xiaoling Zhu, Qun Lu, Hong Yang, Rui Liu

“…Surprisingly, the 16S rRNA sequencing and UHPLC-Q-TOF/MS demonstrated that DHPA and DHAA only slightly disturbed the intestinal microbiome, but significantly altered the urine metabolome of HFD mice mainly by regulating pentose and glucuronate interconversion, tyrosine metabolism, pentose phosphate and tricarboxylic acid (TCA) cycle as indicated by metabolic pathway analysis based on Kyoto Encyclopedia of Genes and Genomes (KEGG) database. …”
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USC-Derived Small Extracellular Vesicles-Functionalized Scaffolds Promote Scarless Vaginal Defect Repair via Delivery of Decorin and DUSP3 Proteins by Xu Y, Li J, Qiu Y, Wu F, Xue Z, Liu B, Fan H, Zhou Y, Wu Q

“…Immunofluorescence staining, Western blot, qRT-PCR and collagen gel contraction analyses were conducted to evaluate the antifibrotic effects of USC-sEVs. RNA sequencing was employed to elucidate the underlying mechanisms involved. …”
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Infectious bursal disease virus affecting interferon regulatory factor 7 signaling through VP3 protein to facilitate viral replication by Zhiyuan Wang, Yang Chen, Yanyan Chen, Rui Chen, Weiwei Wang, Shichen Hu, Yihai Li, Hongjun Chen, Ping Wei, Xiumiao He

“…Infectious bursal disease virus (IBDV) is a double-stranded RNA virus that induces immune suppression and high mortality rates in chickens aged 3-6 weeks. …”
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Interaction Between Malat1 and miR-499-5p Regulates Meis1 Expression and Function with a Net Impact on Cell Proliferation by Salma A. Fahim, Manon Ragheb, Ibrahim Hassan Fayed, Aya Osama, Ahmed Karam, Sameh Magdeldin, Rana Metwale, Mohamed Dief Allah Abdalmoneam Elsayed, Ahmed Abdellatif, Hesham A. Sadek, Shereen Ahmed El Sobky, Nada El-Ekiaby, Injie Omar Fawzy, Ahmed Ihab Abdelaziz

“…For the first time, we demonstrated that the overexpression of miR-499-5p led to the downregulation of Meis1 mRNA and protein in C166 cells by directly binding to its 3’UTR. …”
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Sustained release of miR-21 carried by mesenchymal stem cell-derived exosomes from GelMA microspheres inhibits ovarian granulosa cell apoptosis in premature ovarian insufficiency by Xiaofei Zhang, Linzi Ma, Xiaotong Liu, Xingyu Zhou, Ao Wang, Yunhui Lai, Jun Zhang, Ying Li, Shiling Chen

“…Methods and results: In this study, miR-21 was identified as the predominant miRNA with low-expression in follicular fluid exosomes of POI patients and was shown to possess antiapoptotic activity. …”
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Effects of milk extracellular vesicles from Holstein Friesian and Brown Swiss heat-stressed dairy cows on bovine mammary epithelial cells by S. Castellani, L. Basiricò, A. Maggiolino, C. Lecchi, P. De Palo, U. Bernabucci

“…Cell viability and apoptosis assay, reactive oxygen species production, and mRNA expression of heat shock proteins and antioxidant genes by reverse transcription and real time PCR were determined. …”
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Tousled-like kinase loss confers PARP inhibitor resistance in BRCA1-mutated cancers by impeding non-homologous end joining repair by Min-ah Kim, Banseok Kim, Jihyeon Jeon, Jonghyun Lee, Hyeji Jang, Minjae Baek, Sang-Uk Seo, Dongkwan Shin, Anindya Dutta, Kyung Yong Lee

“…Loss-of-function experiments were performed in multiple human cancer cell lines using siRNA-mediated knockdown or CRISPR-Cas9 gene knockout. …”
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Whole-genome sequencing surveillance of Siberian tick-borne encephalitis virus (TBEV) identifies an additional lineage in Kyrgyzstan by Jake D'Addiego, Mollie Curran-French, Jack Smith, Asankadyr T Junushov, Irena Breininger, Barry Atkinson, John Hay, Roger Hewson

“…Whilst we were able to retrieve the first partial TBEV sequence from Kyrgyzstan from Ixodes persulcatus ticks, we were unable to retrieve a complete genome sequence at that time.In this study, we have utilised a sequence-independent single-primer amplification (SISPA) protocol and retrieved the complete genome sequence of our previous 2009 TBEV tick sample (strain KY09) producing the third complete TBEV genome from Kyrgyzstan, and the first genome from the region clustering within the Vasilchenko lineage, suggesting a wider distribution for the lineage than was previously thought.We have also developed a tiling amplicon scheme for Siberian TBEV (TBEV-Sib) which produced > 90 % reference coverage at 100x sequencing depths for samples with as little as 1.13×104 RNA copies/ml. Since high viral loads are rare in TBEV clinical samples, the developed protocol adds value to TBEV-Sib endemic regions by offering a novel set of primers to further amplify the viral genome prior to sequencing.…”
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吴茱萸次碱通过Sirt1改善血管平滑肌细胞钙化 by 邵静, 陈安, 张秀丽, 杨平珍

“…另外，高磷高钙可下调血管平滑肌细胞Sirt1的表达水平，qRT-PCR和Western blot结果证实吴茱萸次碱可明显地上调Sirt1 mRNA（0.35±0.06、0.75±0.11；0.22±0.08、0.87±0.13；P<0.05）和Sirt1的蛋白（0.38±0.09、0.71±0.13；P<0.05）的表达水平。…”
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Exploring the recombinant evolution and hosts of crucivirus based on novel oyster-associated viruses by Hong-Sai Zhang, Hong-Sai Zhang, Chang Liu, Guang-Feng Liu, Yu-Yu Chen, Peng Zhu, Peng Zhu, Xin Xu, Bing-Xin Yin, Jing-Zhe Jiang, Jing-Zhe Jiang

“…Their capsid proteins share homology with the RNA virus tombusvirus, while their replicase-associated proteins are homologous to a class of single-stranded DNA viruses, namely CRESS DNA viruses. …”
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Gut Microbiome and Metabolite Characteristics Associated With Different Clinical Stages in Non-Small Cell Lung Cancer Patients by Liu F, Lu X, Tang M, Chen Y, Zheng X

“…Subsequently, they performed 16S rRNA gene amplification sequencing and untargeted gas/liquid chromatography-mass spectrometry metabolomics analysis.Results: The study revealed that the alpha-diversity of the gut microbiome in NSCLC patients at different stages did not exhibit statistically significant differences. …”
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Differential Effects of Angelicin Analogues on NF-κB Activity and IL-8 Gene Expression in Cystic Fibrosis IB3-1 Cells by Ilaria Lampronti, Maria Giulia Manzione, Gianni Sacchetti, Davide Ferrari, Susanna Spisani, Valentino Bezzerri, Alessia Finotti, Monica Borgatti, Maria Cristina Dechecchi, Giorgia Miolo, Giovanni Marzaro, Giulio Cabrini, Roberto Gambari, Adriana Chilin

“…Other analogues displayed higher activities; in particular, the most interesting compounds showing relevant anti-inflammatory effects were found to cause 56–83% reduction of IL-8 mRNA expression at low concentrations (1–10 μM), without changes in cell proliferation pattern, demonstrating their potential interest for a possible development of anti-inflammatory therapy of cystic fibrosis.…”
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Genome drafting of nosocomial infection CRE Klebsiella pneumoniae confirming resistance to colistin and eravacycline, carrying blaNDM-1, mcr-1, and blaKPC-2, in neonatology from No... by Xinlong Zhou, Kailash Wagh, Guizhen Lv, Devender Sharma, Wei Lei

“…Identification and antimicrobial susceptibility testing were initially conducted using the Vitek®2 system with an N-395 card and further confirmed by 16S rRNA sequencing. Whole-genome sequencing (WGS) and antimicrobial resistance (AMR) profiling were performed to identify resistance genes and virulence factors. …”
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Increased cardiac macrophages in Sorbs2-deficient hearts: revealing a potential role for macrophage in responding to embryonic myocardial abnormalities by Beibei Hu, Xiangyang Liu, Xiangyang Liu, Xiangyang Liu, Shanshan Xiong, Qin Gong, Junjie Yang, Hongjun Shi, Hongjun Shi, Hongjun Shi, Min Zhang, Fei Liang, Zhen Zhang, Zhen Zhang

“…Previously, we reported that the structural protein Sorbs2 promotes the development of the second heart field, with its deficiency resulting in atrial septal defects (ASD). In analyzing RNA-seq data, we noted an upregulation of macrophage-related genes in Sorbs2−/− hearts. …”
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Functionality of bacterial communities in constructed wetlands used for water purification: influence of root components and seasonality by Yao Huang, Yao Huang, Weili An, Weili An, Tianzhu Ning, Zhiguang Ma, Yuelin Li, Ke Liu, Lingbo Ji, Lingbo Ji, Hongxiao Liu, Dafeng Hui, Hai Ren

“…., rhizosphere, rhizoplane, and endosphere) and their impact on water purification is still limited.MethodsTo address this knowledge gap, we employed high-resolution 16S rRNA deep amplicon sequencing to explore the bacterial community structure and assembly within the root components of three plant species (i.e. …”
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Application of N-Acetyl-Glucosaminil-N-Acetyl-Muramyl Dipeptide during Triple Component Anti-Helicobacter Pylori Therapy in the Period of Coronavirus Infection COVID-19 by M. R. Konorev, E. N. Tyshevich, R. A. Pavlyukov

“…Detection of SARS-CoV-2 RNA by PCR was carried out from April 2020 to April 2022. …”
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Implication of fibroblast growth factor 7 in ovarian cancer metastases and patient survival by Laura F. Mortan, Laura F. Mortan, Brooke A. Meelheim, Justin Garland, Justin Garland, Jacqueline A. Bohn, Zitha Redempta Isingizwe, Doris M. Benbrook, Doris M. Benbrook

“…Significances of differential gene expression and patient prognosis were determined by volcano plot and log-rank test, respectively.ResultsTumor transcriptomes exhibited higher endothelial cells, oxygenation, proteasome activity, and metabolism in comparison to ascites, but similar percentages of cancer-associated fibroblasts and immune cells. FGF7 mRNA was significantly higher in mouse tumors compared to ascites. …”
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