Jiawei Suanzaoren decoction for the treatment of perimenopausal insomnia: clinical observation and experimental study by Li-Qiong Chen, Yong-Can Zhou, Fang Ning, Ming-Jin Zhu

“…The mechanism behind this improvement may be related to the regulation of Estradiol (E2), Follicle-Stimulating Hormone (FSH), and luteinizing hormone (LH) levels, as well as the mRNA expression levels of 5-HT1a, 5-HT2a, GABAARα1, and GABAARγ2 receptors in the hypothalamus.…”
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Genomic 8-oxoguanine modulates gene transcription independent of its repair by DNA glycosylases OGG1 and MUTYH by Tobias Obermann, Teri Sakshaug, Vishnu Vignesh Kanagaraj, Andreas Abentung, Mirta Mittelstedt Leal de Sousa, Lars Hagen, Antonio Sarno, Magnar Bjørås, Katja Scheffler

“…Here, we applied an improved OG-sequencing approach reducing artificial background oxidation and RNA-sequencing to correlate genome-wide distribution of OG with gene transcription in OGG1 and/or MUTYH-deficient cells. …”
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Association of serum and local GRP78 and CHOP expressions with disease progression in patients with non-traumatic osteonecrosis of femoral head by Peng Zhang, Qing-He Ye, Wen-Xiu Zhu, Yong-Heng Zhao, Hong-Xun Zhu, Biao-Fang Wei

“…A total of 42 ONFH patients at FICAT stage 3, 40 patients at FICAT stage 4, and 49 FNF patients received total hip replacement (THR). The mRNA and protein levels of CHOP and GRP78 were elevated in necrotic area compared to the non-necrotic area of ONFH patients and the FH tissues of FNF patients with statistical significance. …”
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Puerariae Radix protects against ulcerative colitis in mice by inhibiting NLRP3 inflammasome activation by Yu Ga, Yuanyuan Wei, Qingyu Zhao, Yimeng Fan, Yannan Zhang, Zhifang Zhang, Sijia Hao, Lixia Wang, Zhifen Wang, Jinlong Han, Shuang Wu, Zhihui Hao

“…PR treatment suppressed upregulation of NOD-like receptor protein 3 (NLRP3), cysteinyl aspartate-specific proteases-1 (caspase-1), apoptosis-associated speck-like (ASC) and gasdermin D (GSDMD) at both the protein and mRNA expression levels. The addition of a small molecule dual-specifi city phosphatase inhibitor NSC 95397 inhibited the positive effects of PR. …”
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An integrated method for taxonomic identif ication of microorganisms by Yu. E. Uvarova, A. V. Bryanskaya, A. S. Rozanov, V. N. Shlyakhtun, E. A. Demidov, K. V. Starostin, T. N. Goryachkovskaya, S. V. Shekhovtsov, N. M. Slynko, S. E. Peltek

“…For accurate species-level identification of microorganisms, researchers today increasingly use a combination of standard microbiological cultivation and visual observation methods with molecular biological and genetic techniques that help distinguish between species and strains of microorganisms at the level of DNA or RNA molecules. The aim of this work was to identify microorganisms from the ICG SB RAS Collection using an integrated approach that involves a combination of various phenotypic and genotypic characteristics. …”
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Effects of cold-pressed wheat germ oil and Bacillus subtilis on growth performance, digestibility, immune status, intestinal microbial enumeration, and gene expression of broilers... by Abdel-Moneim Eid Abdel-Moneim, Safaa A.M. Ali, M.G. Sallam, Ahmed M. Elbaz, Noura M. Mesalam, Zangabel S. Mohamed, AbdelRahman Y Abdelhady, Bing Yang, Mohamed Farouk Elsadek

“…Heat stress exposure adversely affected broiler growth performance, carcass traits, immune response, and insulin-like growth factor 1 (IGF-1) and mucin2 (MUC2) mRNA expression. However, the CWB group showed a lower FCR, reduced mortality rate, and increased BWG compared to the other groups. …”
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Prevalence and pathogenic potential of Shewanella species in oysters and seawater collected from the Chesapeake Bay and Maryland Coastal Bays by Tahirah Johnson, Gary P. Richards, John Jacobs, Howard Townsend, Esam Almuhaideb, Detbra Rosales, Paulinus Chigbu, Ligia Dasilva, Salina Parveen

“…The highest total Shewanella counts were 1.8 × 107 CFU/g in oysters and 4.0 × 102 CFU/mL in seawater. 16S rRNA sequencing was performed on 1,344 representative isolates of which 890 (713 oyster, 177 seawater) were confirmed as Shewanella within 16 species. …”
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Protective Effect of Daikenchuto on Dextran Sulfate Sodium-Induced Colitis in Mice by Takaharu Matsunaga, Shinichi Hashimoto, Naoki Yamamoto, Ryo Kawasato, Tomohiro Shirasawa, Atsushi Goto, Koichi Fujisawa, Taro Takami, Takeshi Okamoto, Jun Nishikawa, Isao Sakaida

“…Colon length; histological scores; and interleukin- (IL-) 10, IL-1β, and tumor necrosis factor-α mRNA expression profiles were analyzed using real-time PCR. …”
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Imbalance of Microbacterial Diversity Is Associated with Functional Prognosis of Stroke by Xintong Zhang, Xiangyu Wang, Hong Zhao, Risheng Cao, Yini Dang, Binbin Yu

“…Gas chromatography was used to determine the fecal SCFAs, and 16S rRNA gene sequencing was used to evaluate their fecal microbiota. …”
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Effect of 25-hydroxycholecalciferol and high phytase doses on performance, vitamin D status, bone mineralization, and mechanistic target of rapamycin gene expression of broilers by L.S. Bassi, C.C.S. Martins, C.A. Lozano-Poveda, I.C. Dias, S.G. Oliveira, A. Maiorka

“…Phytase did not affect growth performance from 1 to 21 d, but a higher dose (2 000 FYT/kg) reduced feed intake and FCR from 22 to 42 d compared to 600 FYT/kg, also reducing FCR in the total period (P < 0.05). The mRNA expression of mechanistic target of rapamycin in breast muscle assessed at 42 d was enhanced with 2 000 FYT/kg (P < 0.001). …”
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Osteocalcin Alleviates Nonalcoholic Fatty Liver Disease in Mice through GPRC6A by Mingliang Zhang, Xiaomin Nie, Yeqing Yuan, Yansu Wang, Xiaojing Ma, Jun Yin, Yuqian Bao

“…Consistent with previous studies, uncarboxylated osteocalcin significantly protected high-fat diet-fed wild-type mice from obesity and NAFLD, while it did not protect high-fat diet-fed GPRC6ALKO mice from NAFLD. Differential mRNA expression of lipogenesis and lipolysis between GPRC6ALKO mice and control mice revealed that GPRC6A mediated the effects of osteocalcin in alleviating NAFLD through inhibiting lipid synthesis and promoting lipolysis. …”
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A Study on the Dietary Yeast Polysaccharide Supplementation in Growth Performance, Antioxidant Capacity, and Immunity of Juvenile Largemouth Bass (<i>Micropterus salmoides</i>) by Junjie Qin, Haifeng Mi, Mingchun Ren, Dongyu Huang, Hualiang Liang, Lu Zhang, Tao Teng, Heng Yin

“…Moreover, the 0.05Y group showed significantly higher immune-related gene (nuclear factor-kappa B, interleukin-8, and interleukin-10) mRNA expression. Altogether, our results indicate that dietary YPS supplementation enhances the antioxidant and immune capacity of <i>M. salmoides</i>, but with no positive effect on their growth.…”
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Wnt signalling facilitates neuronal differentiation of cochlear Frizzled10-positive cells in mouse cochlea via glypican 6 modulation by Man Wang, Yuechen Han, Weibin An, Xue Wang, Fang Chen, Junze Lu, Yu Meng, Yan Li, Yanqing Wang, Jingxin Li, Chunjie Zhao, Renjie Chai, Haibo Wang, Wenwen Liu, Lei Xu

“…Wnt signaling enhances the ability of FZD10 + cells to function as neural progenitors and increases the neuronal excitability of the FZD10–derived neurons. Single-cell RNA sequencing analysis characterizes FZD10-derived differentiating cell populations, while crosstalk network analysis identifies multiple signaling pathways and target genes influenced by Wnt signaling that contribute to the function of FZD10 + cells as neural progenitors. …”
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The prevalence of conjunctivitis in patients with novel coronavirus (COVID-19) and preventive measures by I.R. Gazizova, Yu.A. Desheva, T.V. Gavrilova, V.A. Chereshnev

“…</i> </p> <p> <i>More research is needed to describe the presence of viral RNA in tears and to determine whether SARS-CoV-2 is transmitted by the contact with conjunctiva and/or through secrets although conjunctivitis was diagnosed in only a few patients with COVID-19 and only a few patients were positive for SARS-CoV-2 by conjunctival polymerase chain reaction (PCR).…”
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Lactoferrin Modulates Radiation Response Under Hypoxic Conditions, Possibly Through the Regulation of ROS Production in a Cell Type-Specific Manner by Daitoku Murakami, Takahiro Fukazawa, Michihito Kyo, Mutsumi Miyauchi, Shigehiro Ono, Tomonao Aikawa, Nobuyuki Hirohashi, Keiji Tanimoto

“…Measurements of ROS activity showed that LF decreased ROS production in KD cells but increased it in HSC2 cells, resulting in a decrease in γH2AX foci in KD cells but an increase in HSC2 cells. RNA-seq and gene set enrichment analysis showed that LF treatment regulated gene expression related to the cell cycle, apoptosis, inflammation, and the NRF2 antioxidant signaling pathway. …”
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高原鼠兔和高原鼢鼠肠道微生物组及常见抗生素抗性基因的比较 by 冯天舒 1 俞巧玲 1 周蕊 1 王逸洁 1 苏军虎 2 李欢 1, 3

“…因此，对这两个物种肠道微生物组和抗生素抗性基因的研究十分重要。本研究通过16S rRNA基因测序，定量PCR等技术获得两物种肠道菌群特征并检测到了抗生素抗性基因。结果发现，在门水平上，高原鼢鼠肠道拟杆菌门（Bacteroidetes）的相对丰度更高；而高原鼠兔肠道厚壁菌门（Firmicutes）和放线菌门（Actinobacteria）的相对丰度更高，在属水平上，高原鼢鼠的拟杆菌属（Bacteroides）和S247科中某属的相对丰度更高，而高原鼠兔的普雷沃氏菌（Prevotella）、颤螺菌属（Oscillospira）、YRC22和瘤球菌科（Ruminococcaceae）中某属的相对丰度更高。…”
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Effects of Probiotics Red Yeast Rhodotorula paludigena CM33 on enhancing color pigmentation, antioxidant activity, immune response, intestinal microbiota, and growth in a commercia... by Jirawadee Kaewda, Papungkorn Sangsawad, Surintorn Boonanuntanasarn, Pimpisut Manassila, Apichat Boontawan, Mariena Ketudat‑Cairns, Cheeranan Sriphuttha, Chatsirin Nakharuthai

“…These findings correspond with the mRNA expression results of growth-related genes (insulin-like growth factor-1 (IGF-I) and IGF-II), antioxidant genes (superoxide dismutase (SOD) and catalase (CAT)), and pro-inflammatory genes (interleukin-1 beta (IL-1β) and IL-6). …”
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Long-term effects of combining anaerobic digestate with other organic waste products on soil microbial communities by Daniela Mora-Salguero, Denis Montenach, Manon Gilles, Vincent Jean-Baptiste, Sophie Sadet-Bourgeteau, Sophie Sadet-Bourgeteau

“…Soil microbial communities were characterized using high-throughput sequencing targeting 16S and 18S ribosomal RNA genes, following DNA extraction from soil samples collected in 2022, six years after the initial digestate application.ResultsThe results indicated that combining OWPs rich in stable and recalcitrant organic matter, such as BIO and FYM, with raw digestate, offers an improved fertilization practice. …”
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Spinal CRH facilitates the micturition reflex via the CRH2 receptor in rats with normal bladder and bladder outlet obstruction by Risa Shinoki, Ryosuke Jikuya, Takuma Nirei, Takeshi Fukazawa, Hiroki Takizawa, Mari Hioki, Sachi Kawaura, Tomoyuki Tatenuma, Go Noguchi, Daiki Ueno, Yusuke Ito, Mitsuru Komeya, Kentaro Muraoka, Hisashi Hasumi, Kazuki Kobayashi, Masahito Takiguchi, Kengo Funakoshi, Kazuhide Makiyama, Naoki Aizawa, Hiroki Ito

“…Real-time PCR showed higher CRHR2 mRNA expression in the spinal cord and DRG than in the bladder in both groups. …”
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A novel approach to regulate glucose uptake in an anaplastic thyroid cancer cell line by Shabnam Heydarzadeh, Ali Asghar Moshtaghie, Maryam Daneshpour, Mehdi Hedayati

“…Determination of GLUT1 and GLUT3 mRNA and protein expression in SW1736 cells was performed by qRT-PCR and western blotting. …”
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