N88S seipin-related seipinopathy is a lipidopathy associated with loss of iron homeostasis by Mariana O. Ribeiro, Mafalda Oliveira, Verónica Nogueira, Vítor Costa, Vitor Teixeira

“…This was linked to changes in the expression of Aft1p-controlled iron regulon genes, including the mRNA-binding protein CTH2 and the high-affinity iron transport system member FET3, in a p38/Hog1p- and Msn2p/Msn4p-dependent manner. …”
Get full text

Immigration reduces selection in water microbial community assembly by Fen-Guo Zhang, Kefan Wu, Sanqing Zhang, Furong Liang, Zhihua Du, Yongji Wang, Quan-Guo Zhang

“…The diversity, structure, and composition of the bacterial community in the water were assessed using 16S rRNA gene amplicon sequencing on the 13th and 60th days after the start of the experiment. …”
Get full text

Modulation of the endocannabinoid system by (S)-ketamine in an animal model of depression by Nicole R. Silva, Shokouh Arjmand, Luana B. Domingos, Adriano M. Chaves-Filho, Melina Mottin, Caroline C. Real, Anna L. Waszkiewicz, Pedro H. Gobira, Alessio Nicola Ferraro, Anne M. Landau, Carolina H. Andrade, Heidi K. Müller, Gregers Wegener, Sâmia R.L. Joca

“…Changes in eCB receptors and enzymes were assessed at mRNA and protein levels (qPCR and western blot), CB1 binding ([3H]SR141716A autoradiography) and endocannabinoid content (lipidomics). …”
Get full text

Influence of Titanium Alloy Scaffolds on Enzymatic Defense against Oxidative Stress and Bone Marrow Cell Differentiation by Lais Morandini Rodrigues, Elis Andrade Lima Zutin, Elisa Mattias Sartori, Daniela Baccelli Silveira Mendonça, Gustavo Mendonça, Yasmin Rodarte Carvalho, Luana Marotta Reis de Vasconcellos

“…At day 14, all scaffolds had more than 2-fold induction for Runx2 mRNA levels, with statistically significant differences compared to the control group. …”
Get full text

Decreased Frequencies of Th17 and Tc17 Cells in Patients Infected with Avian Influenza A (H7N9) Virus by Jiaqi Bao, Dawei Cui, Xiaochen Wang, Qianda Zou, Dejian Zhao, Shufa Zheng, Fei Yu, Li Huang, Yuejiao Dong, Xianzhi Yang, Guoliang Xie, Weizhen Chen, Yu Chen

“…In this study, we found that the frequencies of Th17 and Tc17 cells among human peripheral blood mononuclear cells (PBMCs) as well as IL-17A protein and mRNA levels were markedly decreased in patients with acute H7N9 virus infection. …”
Get full text

Spatial tumor immune heterogeneity facilitates subtype co-existence and therapy response in pancreatic cancer by Lukas Klein, Mengyu Tu, Niklas Krebs, Laura Urbach, Daniela Grimm, Muhammad Umair Latif, Frederike Penz, Anna Blandau, Xueyan Wu, Rebecca Diya Samuel, Stefan Küffer, Florian Wegwitz, Nathan Chan, Kazeera Aliar, Foram Vyas, Uday Kishore, Elisabeth Hessmann, Andreas Trumpp, Elisa Espinet, Argyris Papantonis, Rama Khokha, Volker Ellenrieder, Barbara T. Grünwald, Shiv K. Singh

“…Here, by combining preclinical models, multi-center clinical, transcriptomic, proteomic, and patient bioimaging data, we identify an interplay between neoplastic intrinsic AP1 transcription factor dichotomy and extrinsic macrophages driving subtype co-existence and an immunosuppressive microenvironment. ATAC-, ChIP-, and RNA-seq analyses reveal that JUNB/AP1- and HDAC-mediated epigenetic programs repress pro-inflammatory signatures in tumor cells, antagonizing cJUN/AP1 signaling, favoring a therapy-responsive classical neoplastic state. …”
Get full text

Transcriptome analysis reveals modulations in glycosylation profiles of the mucosal barrier and their potential interaction with gut microbiota in weaned piglets by Hao Cheng, Hao Li, Yujie Zhao, Kai Yang, Jing Wang, Bie Tan, Xiaokang Ma

“…DLY piglets displayed elevated mRNA expression levels of MUC2 and MUC5AC in colonic mucosal tissue than NX piglets (P < 0.05). …”
Get full text

CD14loCD301b+ macrophages gathering as a proangiogenic marker in adipose tissues by Yibing Lv, Yidan Zheng, Shanshan Su, Junyi Xiao, Jie Yang, Lingyun Xiong, Yanyan Guo, Xiaoqi Zhou, Nengqiang Guo, Ping Lei

“…To investigate the underlying mechanisms, adipose tissues (ATs) from Cd14−/− and WT mice were subjected to deep RNA sequencing. Gene Ontology enrichment analysis revealed a significant enhancement of angiogenesis-related function in the Cd14−/− epididymal adipose tissues compared to WT counterpart, which was accompanied by an upregulation of Cd301b. …”
Get full text

Serological insights from SARS-CoV-2 heterologous prime and boost responses in Thailand by Daniel Ward, Lapasrada Pattarapreeyakul, Rujiraporn Pitaksalee, Naphatcha Thawong, Waritta Sawaengdee, Suthida Tuntigumthon, Catriona Patterson, Kevin Tetteh, Susana Campino, Panadda Dhepakson, Surakameth Mahasirimongkol, Taane G. Clark

“…The Thailand Ministry of Health adopted these strategies using vector, inactivated, and mRNA vaccines. However, this approach has introduced challenges for SARS-CoV-2 sero-epidemiology studies. …”
Get full text

Combination Fractional Carbon Dioxide Laser Treatment and Bone Marrow Mesenchymal Stem Cell Therapy Enhances the Treatment of Skin Photoaging in a Murine Model System by Li L, He Z, Yu C, Zhang C, Yu Y, Li Y, Xu X

“…In addition, immunohistochemical analyses and real-time polymerase chain reaction analyses were conducted to detect MMP-3 and MMP-9 expression.Results: After 1 week, both dermal thickness and collagen fiber density were significantly increased in the BMSC and combination treatment groups as compared to the control group (P< 0.05), while both of these parameters were significantly increased in all treatment groups after 4 weeks relative to the control group (P< 0.05), with the most pronounced effect in the combination therapy group (P< 0.05). MMP-3 and MMP-9 mRNA and protein levels in the treatment groups were decreased relative to the control group after 4 weeks.Conclusion: Combination BMSC and carbon dioxide laser therapy was more effective than either of these therapeutic approaches in isolation as a treatment for photoaged skin. …”
Get full text

Kaempferol protects against high-salt-induced hypertension and vascular endothelial injury by inhibiting ferroptosis through the ATF4/ACSL4 pathway by Qiuer Liang, Minghao Chen, Guangtian Chen, Pengli Xu, Lai Kwan Lam, Pengcheng Xie, Ting Xie, Wanqing Tu, Tianhao Liu, Xiaopeng Peng, Haoyao Yuan, Liguo Chen, Ya Xiao

“…Additionally, human aortic endothelial cells (HAECs) cultured under high-salt conditions were used to assess Kae's protective effects, with ATF4 and acyl-CoA synthetase long-chain family member 4 (ACSL4) knocked down via siRNA transfection. Our results showed that Kae significantly reduced high-salt-induced hypertension, improved vascular endothelial dysfunction, inhibited ferroptosis, and suppressed ATF4 expression. …”
Get full text

Neuroligin 1 Regulates Autistic‐Like Repetitive Behavior through Modulating the Activity of Striatal D2 Receptor‐Expressing Medium Spiny Neurons by Dandan Lv, An Liu, Ziyue Yi, Mingdao Mu, Miao Wu, Xingcan Li, Kun Cao, Ruining Liu, Zhengping Jia, Junhai Han, Wei Xie

“…Finally, through single‐nucleus RNA sequencing (sn‐RNAseq) and protein detection verification, it is revealed that the overactivation of protein kinase C (PKC) in Nlgn1‐deficient mice contributes to excessive repetitive behaviors and increased neuronal excitability. …”
Get full text

MiR-127-3p靶向MAPK4对葡萄膜黑色素瘤细胞增殖、凋亡、迁移和侵袭的影响 by 魏丽, 连红梅, 刘鹏, 刘兴华, 吴书一, 刘萌萌

“…【方法】通过RT-qPCR检测人葡萄膜黑色素瘤组织及细胞、正常组织及细胞中miR-127-3p与MAPK4mRNA的表达；通过Lipofectamine2000说明书将mimic-NC、miR-127-3pmimic、pc-MAPK4质粒分别或联合转染进入SP6.5或OM431细胞；通过双荧光素酶报告检测miR-127-3p与MAPK4复染靶向关系；通过CCK-8法检测细胞增殖，流式细胞术检测细胞凋亡，划痕实验检测细胞迁移能力，Transwell法检测细胞侵袭能力，蛋白印迹法检测AKT/mTOR通路蛋白相对表达水平。…”
Get full text

Corneal protective effects of a new ophthalmic formulation based on vitamin B12 and sodium hyaluronate by Francesca Lazzara, Federica Conti, Grazia Maugeri, Velia D’Agata, Velia D’Agata, Ludovica Sotera, Claudio Bucolo, Claudio Bucolo

“…Cell viability, cytotoxicity, ROS production, and mRNA expression of pro-inflammatory cytokines (TNF-α and IL-1β) were assessed by MTT, LDH, 2′,7′–dichlorofluorescein diacetate (DCFDA) assays and Real-time PCR, respectively. …”
Get full text

高山兀鹫肠道微生物的分离鉴定与耐药性分析 by 候建平 郑思思 龙鑫 海佳怡 王稳 朱丽琳

“…为了解高山兀鹫肠道微生物的多样性及其耐药情况，本研究采集野生高山兀鹫的新鲜粪便在实验室进行细菌分离培养、16S rRNA基因PCR扩增和序列分析、生化鉴定以及细菌耐药性试验。结果显示：从高山兀鹫新鲜粪便中共分离到119株细菌，隶属于6个属、8个种，分别是大肠埃希氏菌（Escherichia coli）、藤黄微球菌（Micrococcus luteus）、奥斯陆莫拉氏菌（Moraxella osloensis）、蜡样芽孢杆菌（Bacillus cereus）、索氏类梭菌（Paeniclostridium sordellii）、蒙氏肠球菌（Enterococcus mundtii）、海氏肠球菌（Enterococcus hirae）以及耐久肠球菌（Enterococcus durans）。…”
Get full text

Dual inhibition of BET and EP300 has antitumor activity in undifferentiated pleomorphic sarcomas and synergizes with ferroptosis induction by Stéphanie Verbeke, Aurélien Bourdon, Mathilde Lafon, Vanessa Chaire, Bertolo Frederic, Amina Naït Eldjoudi, Marie-Alix Derieppe, Francis Giles, Antoine Italiano

“…The most potent inhibitor was evaluated in vivo in two animal models and the mechanisms of action were investigated by RNA sequencing, Western blotting and immunofluorescence staining. …”
Get full text

Low-dose intranasal deferoxamine modulates memory, neuroinflammation, and the neuronal transcriptome in the streptozotocin rodent model of Alzheimer’s disease by Jared M. Fine, Jacob Kosyakovsky, Tate T. Bowe, Katherine A. Faltesek, Benjamin M. Stroebel, Juan E. Abrahante, Michael R. Kelly, Elizabeth A. Thompson, Claire M. Westby, Kiley M. Robertson, William H. Frey, Leah R. Hanson

“…Furthermore, IN DFO modulated several aspects of the neuroinflammatory milieu of the ICV STZ model, which was assessed through a novel panel of brain cytokines and immunohistochemistry. Using RNA-sequencing and pathway analysis, STZ was shown to induce several pathways of cell death and neuroinflammation, and IN DFO engaged multiple transcriptomic pathways involved in hippocampal neuronal survival.DiscussionTo our knowledge this study is the first to assess the transcriptomic pathways and mechanisms associated with either the ICV STZ model or DFO treatment, and the first to demonstrate efficacy at this low dose.…”
Get full text

Expression of Sclerostin in Osteoporotic Fracture Patients Is Associated with DNA Methylation in the CpG Island of the SOST Gene by Yanming Cao, Bin Wang, Ding Wang, Dongxiang Zhan, Caiyuan Mai, Peng Wang, Qiushi Wei, Yamei Liu, Haibin Wang, Wei He, Liangliang Xu

“…After extracting total RNA and protein, we checked the relative expression levels of SOST by quantitative real-time PCR and western blot. …”
Get full text

Identification and Characterization of Genes Associated with Intestinal Ischemia-Reperfusion Injury and Oxidative Stress: A Bioinformatics and Experimental Approach Integrating Hig... by Xie Y, Yang M, Huang J, Jiang Z

“…A mouse IIRI model and ROC curve were employed to verify the accuracy of HIOSRGs. Finally, siRNA was utilized to suppress the expression of HDAC3 in Caco2 cells, and the changes in oxidative stress levels before and after hypoxia-reoxygenation in Caco2 cells were observed.Results: A total of 277 OSRGs and 4 HIOSRGs were obtained. …”
Get full text

Association of Adult Atopic Dermatitis with Impaired Oral Health and Oral Dysbiosis: A Case-Control Study by Aaya Shahin, Yael Anne Leshem, Yossi Taieb, Sharon Baum, Aviv Barzilai, Danielle Jeddah, Efrat Sharon, Omry Koren, Rinat Tzach-Nahman, Shunit Coppenhagen-Glazer, Ronen Hazan, Yael Houri-Haddad, Shoshana Greenberger

“…A dentist assessed oral health status, and oral flora samples were collected and subjected to 16S rRNA sequencing for microbiome analysis. Results: Forty-five AD participants and 41 non-AD controls were recruited. …”
Get full text