Immune regulatory genes impact the hot/cold tumor microenvironment, affecting cancer treatment and patient outcomes by Mengmeng Sang, Jia Ge, Juan Ge, Juan Ge, Gu Tang, Qiwen Wang, Jiarun Wu, Liming Mao, Liming Mao, Xiaoling Ding, Xiaorong Zhou

“…Additionally, hub genes that regulate the tumor microenvironment were identified, and their expression patterns were analyzed using single-cell RNA sequencing. Furthermore, drug sensitivity and molecular docking analyses were performed to identify potential drug candidates capable of transforming cold tumors into hot tumors. …”
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Characterization of mammary glands and milk fat globule transcripts in lactating buffalo and goats by Hancai Jiang, Xiaoxian Xu, Shuwan Wang, Xinhui Song, Ling Li, Qingyou Liu, Kuiqing Cui, Deshun Shi, Jian Wang, Hui Li, Jieping Huang, Zhipeng Li

“…Here, we aimed to reveal the universal rule of the transcript profiles of the milk fat globules and mammary glands from buffaloes and goats by RNA-sequencing analysis. Results showed that, in buffalo, 97 % of mRNAs were expressed in both milk fat globules and mammary glands, with 45 % showing differential expression. …”
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Neuroinflammation mediates the progression of neonate hypoxia-ischemia brain damage to Alzheimer’s disease: a bioinformatics and experimental study by Shengjie Zhang, Ruqiu Zhang, Zhaoqin Chen, Zihan Shao, An Li, Fan Li, Fang Huang

“…After identifying the common differentially expressed genes (DEGs) of Alzheimer’s disease and neonate Hypoxia-Ischemia Brain Damage by limma package analysis, five kinds of analyses were performed on them, namely Gene Ontology (GO) and pathway enrichment analysis, protein–protein interaction network, DEG-transcription factor interactions and DEG-microRNA interactions, protein-drug interactions and protein-disease association analysis, and gene-inflammation association analysis and protein-inflammation association analysis.ResultsIn total, 12 common DEGs were identified including HSPB1, VIM, MVD, TUBB4A, AACS, ANXA6, DIRAS2, RPH3A, CEND1, KALM, THOP1, AREL1. …”
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Nucleic Acid, Antibody, and Virus Culture Methods to Detect Xenotropic MLV-Related Virus in Human Blood Samples by M. F. Kearney, K. Lee, R. K. Bagni, A. Wiegand, J. Spindler, F. Maldarelli, P. A. Pinto, W. M. Linehan, C. D. Vocke, K. A. Delviks-Frankenberry, R. W. deVere White, G. Q. Del Prete, J. W. Mellors, J. D. Lifson, V. N. KewalRamani, V. K. Pathak, J. M. Coffin, S. F. J. Le Grice

“…Analysis of XMRV-spiked samples and/or specimens from two pigtail macaques experimentally inoculated with 22Rv1 cell-derived XMRV confirmed the ability of the assays used to detect XMRV RNA and DNA, and culture isolatable virus when present, along with XMRV reactive antibody responses. …”
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In silico analysis of hub genes and regulatory networks implicates the putamen in non-motor Parkinson’s disease disorders by Stephen Tunmise Akanbi, Joshua Ayodele Yusuf, Thompson Oluwaferanmi Ayandele, Dayo Samson Oladipupo, Darasimi Racheal Olorunlowu, Blessing Dorcas Olawuyi, Joshua Oluwafisayo Adepoju, Dolapo Emmanuel Opasina, Elizabeth Kehinde Opoola, Uchenna Victor Ugwuanyi, Olufunto Omodele Adeleye

“…Also, we constructed transcription factor (TF)-hub gene expression networks, miRNA-hub gene expression networks, and disease hub gene association networks using the JASPAR database, Tarbase database, and DisGeNET via NetworkAnalyst platform, respectively. …”
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Isolation, Identification, and Antimicrobial Susceptibilities of Bacteria from the Conjunctival Sacs of Dogs with Bacterial Conjunctivitis in Different Regions of Wuhan, China by Yuxin Li, Yinan Wang, Xin Gao, Lihong Luo, Bohan Zhang, Xiao Wang, Jing Li, Ruijia Wu, Lixin He, Wenxuan Li, Changwei Qiu

“…The expression of two aminoglycoside modifying enzyme genes, <i>aacA-aphD</i> and <i>aac (6′)-Ib</i>, and three 16S rRNA methyltransferase genes, <i>rmtB</i>, <i>rmtE</i> and <i>npmA</i>, were analyzed by PCR. …”
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Drosophila ubiquitin-specific peptidase 14 stabilizes the PERIOD protein by regulating a ubiquitin ligase SLIMB by So Who Kang, Jung-Eun Park, Soonhyuck Ok, Minhui Um, Hyeonjeong Son, Seunghee Byun, Nayoung Park, Su Jin Lee, Thị Xuân Thùy Trần, Gyeongmin Kim, Jeonghun Yeom, Kyunggon Kim, Eun Young Kim, Min-Ji Kang

“…We find that overexpression of USP14 in clock cells reduces PER protein levels without altering its mRNA levels whereas USP14 knockdown increases PER protein levels, suggesting that USP14 regulates PER post-translationally. …”
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Antiviral Activity and Underlying Mechanism of <i>Moslae herba</i> Aqueous Extract for Treating SARS-CoV-2 by Yan Feng, Qiong Ge, Jian Gao, Zhuoying Wu, Yunyi Zhang, Haiyan Mao, Beibei Wu, Changping Xu

“…Of the nine, the PNP and TPI1 were identified as core targets as AEMH treatment significantly regulated the mRNA expression level of the two genes on infected cells. …”
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Anti-inflammatory treatment using alpha melanocyte stimulating hormone (α-MSH) does not alter osteoblasts differentiation and fracture healing by Johanna Graue, Melanie Timmen, Katharina Schmitz, Daniel Kronenberg, Markus Böhm, Kishor K. Sivaraj, M. Gabriele Bixel, Richard Stange

“…Using quantitative real time PCR, we analyzed mRNA expression of receptors. To explore the impact of α-MSH on bone regeneration in vivo, treatment with α-MSH or NaCl (control) was performed in a murine fracture-healing model using a closed femur fracture stabilized with an intramedullary implant (female, n = 6–8 mice per group). …”
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Modulation of Innate Immunity by G-CSF and Inflammatory Response by LBPK95A Improves the Outcome of Sepsis in a Rat Model by Haoshu Fang, Chuanfeng Hua, Stefanie Weiss, Anding Liu, Wenhui Cheng, Ralf Claus, Jürgen Rödel, Olaf Dirsch, Uta Dahmen

“…Irrespective of dose, the blockade of the LPS-LBP interaction was associated with low systemic cytokine levels and delayed increases in hepatic TNF-α and IL-6 mRNA expression. The delayed increase in cytokines was associated with the phosphorylation of STAT3 and AKT. …”
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Stimulus-activated ribonuclease targeting chimeras for tumor microenvironment activated cancer therapy by Yuqi Zhang, Jinfeng Zhu, Ling Qiu, Zhengzhong Lv, Zhongsheng Zhao, Xingxiang Ren, Yirui Guo, Yan Chen, Miao Li, Yurong Fan, Zhixin Han, Yiming Feng, Haibin Shi

“…Abstract RNA degradation using ribonuclease targeting chimeras (RiboTACs) is a promising approach for cancer therapy. …”
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EGCG improve meat quality, restore lipid metabolism disorder and regulate intestinal flora in high-fat fed broilers by Lujia Gao, Chen Liu, Jiaqi Wu, Ying Cui, Man Zhang, Chongpeng Bi, Anshan Shan, Xiujing Dou

“…Furthermore, EGCG significantly decreased inflammation levels and oxidative damage in high-fat diet-induced obese chickens (P < 0.05). 16S rRNA gene sequencing revealed that dietary supplementation of EGCG reduced the abundance of Bacteroidota and Dielma, while increasing the abundance of Firmicutes, Turiciactor, Romboutsia, and Parasutterella, thereby modulating the microbial composition. …”
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Attenuated titin protein expression is associated with advanced stages of ovarian cancer by Harvey Sharma, Jaskaran Aujla, Asad Nawaz, Thabet Khasawneh, Ayesha Alvero, Robert T. Morris, Asma Basha, Laila Tutunji, Toshima Z. Parris, Khalil Helou, Ghassan M. Saed

“…Quantitative real-time PCR analyzed titin mRNA in cell lines, including chemosensitive, chemoresistant, and normal ovarian cells. …”
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Molecular and Clinical Characterization of Giardia duodenalis Infection in Preschool Children from Lisbon, Portugal by Filipa Santana Ferreira, Rita Alexandre dos Santos Soares de Bellegarde Machado Sá da Bandeira, Cláudia Alexandra Cecílio de Sampaio Ferreira Constantino, Ana Maria Teixeira Duarte Cancela da Fonseca, Joana da Graça Matias Gomes, Rúben Miguel Lopes Rodrigues, Jorge Luís Marques da Silva Atouguia, Sónia Chavarria Alves Ferreira Centeno-Lima

“…Genotyping analysis targeting ssu-rRNA and β-giardin loci revealed six infections with assemblage A and 3 with assemblage B. …”
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Nr5a2 promotes cancer stem cell properties and tumorigenesis in nonsmall cell lung cancer by regulating Nanog by Ting Ye, Jingyuan Li, Zhiwei Sun, Yongli Liu, Liangsheng Kong, Shixia Zhou, Junlin Tang, Jianyu Wang, H. Rosie Xing

“…Using the LLC‐SD model, through transient and stable siRNA interference of Nr5a2 expression, we provided convincing evidence for a regulatory role of Nr5a2 in the maintenance of lung CSC self‐renewal and stem cell properties in vitro. …”
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Identification of microorganisms at different times in a bioleaching process for the recovery of gold and silver from minerals in oxide form by Cuauhtémoc Contreras Mora, Juan Antonio Rojas Contreras, Mayra Cristina Rosales Villarreal, José Luis Urban Martínez, Efren Delgado, Hiram Medrano Roldan, Felipe Samuel Hernández Rodarte, Damián Reyes Jáquez

“…Four native microorganisms were identified using molecular biology techniques and a 16S rRNA gene fragment: Acidovorax citrulli, Brevundimonas albigilva, Sphingomonas korenensis, and Methylobacterium organophilum. …”
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The imprinted Igf2-Igf2r axis is critical for exosome biogenesis during the early development of bovine placenta by Kunhua ZHENG, Longfei XIAO, Naihan YUAN, Xihui SHENG, Xiaolong QI, Yingqiu WANG, Chang CHEN, Kaijun GUO, Lin YANG, Bingying LIU, Xiangguo WANG

“…Furthermore, the expression of exosomal marker factors was detected after the expression of IGF2R or IGF2 was inhibited through RNA interference or the addition of inhibitors, respectively. …”
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Modulation of Caecal Microbiome in Obese Mice Associated with Administration of Amaranth or Soybean Protein Isolates by Diana Olguín-Calderón, Jorge L. González-Escobar, Rosalva Ríos-Villa, Elena Dibildox-Alvarado, Antonio De León-Rodríguez, Ana P. Barba de la Rosa

“…Microbiota was isolated from the caecum and 16S rRNA gene was sequenced. Caecal Short Chain Fatty Acids (SCFAs) were quantified by gas chromatography. …”
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Time-Qualified Patterns of Variation of PPARγ, DNMT1, and DNMT3B Expression in Pancreatic Cancer Cell Lines by Valerio Pazienza, Francesca Tavano, Massimo Francavilla, Andrea Fontana, Fabio Pellegrini, Giorgia Benegiamo, Vincenzo Corbo, Fabio Francesco di Mola, Pierluigi Di Sebastiano, Angelo Andriulli, Gianluigi Mazzoccoli

“…We investigated the time-related patterns of PPARG, DNMT1, and DNMT3B expression monitoring their mRNA levels by qRT-PCR at different time points over a 28-hour span in BxPC-3, CFPAC-1, PANC-1, and MIAPaCa-2 PC cells after synchronization with serum shock. …”
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CXCR4 promotes tumor stemness maintenance and CDK4/6 inhibitors resistance in ER-positive breast cancer by Qiangfeng Shi, Wang Yang, Yiye Ouyang, Yujie Liu, Zijie Cai

“…Recent research has identified several dysregulated genes in CDK4/6 inhibitors-resistant breast cancer, but the underlying mechanism is complex due to tumor heterogeneity and warrants further investigation. Methods RNA sequencing and KEGG pathway analysis was carried out to identify the mainly dysregulated genes in CDK4/6 inhibitors-resistant breast cancer cells. …”
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