Exploring the functional role of POU5F1 using CRISPR RNP electroporation of zygote (CRISPR-EZ) in buffaloes by Meeti Punetha, Dharmendra Kumar, Suman Choudhary, Sheetal Saini, Kamlesh Kumari Bajwa, Surabhi Sharma, Manu Mangal, P. S. Yadav, T. K. Datta

“…We targeted the buffalo POU5F1 gene using the abovementioned parameters, which caused nonsense-mediated mRNA decay and led to complete knockout (KO) of the POU5F1 gene. …”
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Integration of metabolomic and transcriptomic analyses reveals regulatory functions of the ChREBP transcription factor in energy metabolism by Jie An, Inna Astapova, Guofang Zhang, Andrew L. Cangelosi, Olga Ilkayeva, Hannah Marchuk, Michael J. Muehlbauer, Tabitha George, Joseph Brozinick, Mark A. Herman, Christopher B. Newgard

“…Integrated transcriptomic and metabolomic analyses in rats with GalNac-siRNA-mediated suppression of ChREBP expression in liver reveal other ChREBP functions. …”
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Low prevalence of copy number variation in pfmdr1 and pfpm2 in Plasmodium falciparum isolates from southern Angola by Denise Duarte, Francisco Manuel, Ana Dias, Esmeralda Sacato, Elsa Taleingue, Elsa Daniel, Francisco Simão, Luis Varandas, Maria Lina Antunes, Fatima Nogueira

“…António Agostinho Neto (HCL) in Lubango, Angola. qPCR targeting the small-subunit 18S rRNA gene was used to confirm P. falciparum infection. …”
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Genetic profiling of multidrug-resistant Acinetobacter baumannii from a tertiary care center in Malaysia by Aisyah Syakirah Shahari, Navindra Kumari Palanisamy, Fadzilah Mohd Nor

“…The isolates were identified by Vitek 2 and PCR amplification of the 16S rRNA gene followed by sequencing. The isolates were tested against imipenem, ceftazidime, amikacin, gentamicin, ampicillin, and ciprofloxacin using disk diffusion, Epsilometer test, and broth microdilution. …”
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Characterization of the complete genome sequence of the recombinant norovirus GII.P16/GII.4_Sydney_2012 revealed in Russia by E. V. Zhirakovskaia, A. Y. Tikunov, S. N. Sokolov, B. I. Kravchuk, E. I. Krasnova, N. V. Tikunova

“…These small non-envelope viruses with a single-stranded (+)RNA genome are characterized by high genetic variability. …”
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In Vivo Tracking of Chemokine Receptor CXCR4-Engineered Mesenchymal Stem Cell Migration by Optical Molecular Imaging by Senthilkumar Kalimuthu, Ji Min Oh, Prakash Gangadaran, Liya Zhu, Ho Won Lee, Ramya Lakshmi Rajendran, Se hwan Baek, Yong Hyun Jeon, Shin Young Jeong, Sang-Woo Lee, Jaetae Lee, Byeong-Cheol Ahn

“…MSCs were transduced with retroviruses to generate CXCR4-overexpressing MSCs (MSC-CXCR4/Fluc2) and MSCs (MSC/Fluc2). CXCR4 mRNA and protein expression was confirmed by RT-PCR and Western blotting, respectively, and it was higher in MSC-CXCR4/Fluc2 than in naive MSCs. eGFP expression was confirmed by confocal microscopy. …”
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Clinical significance and pro-oncogenic function of DBF4 in clear cell renal cell carcinoma by Liuyan Chen, Lvying Wu, Minying Tang, Yuanhang Cheng, Kuanyin Wang, Jianan Zhang, Wenyi Deng, Lingfeng Zhu, Jin Chen

“…Various assays, including assessments of cell proliferation, apoptosis, the cell cycle, cell migration and invasion, and colony formation, and xenograft tumor models were subsequently performed following to the knockdown of DBF4 expression via shRNA. Results Bioinformatics analyses revealed that DBF4 is significantly overexpressed in ccRCC tissues compared with adjacent normal tissues. …”
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HnRNPM modulates alternative splicing in germ cells by recruiting PTBP1 by Peng Lv, Wenchao Xu, Sheng Xin, Yuanxuan Deng, Bin Yang, Dengjianyi Xu, Jian Bai, Deilin Ma, Tao Wang, Jihong Liu, Xiaming Liu

“…Coimmunoprecipitation and mass spectrometry analyses revealed the proteins that interact with hnRNPM. RNA sequencing was performed to analyse the different alternative splicing events in the testes of control and hnRNPM-deficient mice. …”
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The Effects of MicroRNAs on Cardiomyopathy in a Rat Model of Streptozotocin-induced Diabetes Mellitus by Aslıhan Şeyda DOĞAN, Mukaddes PALA, Şenay GÖRÜCÜ YILMAZ, Ayfer BECEREN, Aydın KARABULUT, Yalçın POLAT, Hatice Kübra ELÇİOĞLU

“…Results: As a result of the validation, it was determined that three miRNAs (rno-miR-15b-5p, rno-miR-196b-3p, and rno-miR-208a-5p) were downregulated, one miRNA (rno-miR-200c-3p) was upregulated (p<0.05). …”
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Mechanism of Mongolian Medicine Batri-7 on Salmonella Enteritis by Yang D, Chen S, Borijihan H, Aoqier A, Sarula S, Siqin S, Manda M, Temuqile T, Baigude H

“…Simultaneously, the Minimum Inhibitory Concentration of BT-7 was evaluated against bacterial by microbroth dilution method, BT-7 regulation of Salmonella typhimurium gene was performed by RNA-Seq methods and verified by qRT-PCR.Results: In the LC-MS/MS assay, negative and positive-ion modes are identified for 511 and 699 compounds from BT-7, respectively. …”
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Epidermal stem cell derived exosomes-induced dedifferentiation of myofibroblasts inhibits scarring via the miR-203a-3p/PIK3CA axis by Shixin Zhao, Haoran Kong, Dahu Qi, Yushuang Qiao, Yu Li, Zhiming Cao, Hanwen Wang, Xuefeng He, Hengdeng Liu, Hao Yang, Suyue Gao, Tao Liu, Julin Xie

“…Epidermal stem cell-derived extracellular vesicles (EpiSC-EVs) were isolated via ultracentrifugation and filtration, followed by miRNA sequencing to identify miRNAs targeting key molecules. …”
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Rare Taxa Are Key Links in Regional Antimicrobial Resistance Profiles in Dusts Across Diverse North American Regions by Paul B. L. George, Florent Rossi, Marc Veillette, Amélia Bélanger Cayouette, Samantha Leclerc, Cindy Dumais, Nathalie Turgeon, Caroline Duchaine

“…Bacterial biomass was assessed using standard qPCR methods of the 16S rRNA gene, which was also used to assess bacterial biodiversity via metabarcoding. …”
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Strawberry dietary intervention influences diversity and increases abundances of SCFA-producing bacteria in healthy elderly people by Franziska Meiners, Bernd Kreikemeyer, Patrick Newels, Ingmar Zude, Michael Walter, Alexander Hartmann, Daniel Palmer, Georg Fuellen, Israel Barrantes

“…Participants in five groups consumed varying amounts of strawberries, freeze-dried strawberries, and capers in olive oil over 10 weeks as part of a randomized controlled trial. 16S rRNA sequencing was used to analyze differences in microbial composition, diversity, phenotypes, differential abundance, and functional pathways. …”
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Extracellular Vesicles from Amnion-Derived Mesenchymal Stem Cells Ameliorate Hepatic Inflammation and Fibrosis in Rats by Masatsugu Ohara, Shunsuke Ohnishi, Hidetaka Hosono, Koji Yamamoto, Kohei Yuyama, Hideki Nakamura, Qingjie Fu, Osamu Maehara, Goki Suda, Naoya Sakamoto

“…AMSC-EVs significantly decreased the number of Kupffer cells (KCs) in the liver of rats with NASH and the mRNA expression levels of inflammatory cytokines such as tumor necrosis factor- (Tnf-) α, interleukin- (Il-) 1β and Il-6, and transforming growth factor- (Tgf-) β. …”
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The transcriptional regulation effects of histidine, isoleucine and glutamate on free exopolysaccharide biosynthesis of Streptococcus thermophilus 937 by Yunchao Wa, Yunchao Wa, Xia Zhao, Xia Zhao, Chenchen Zhang, Chenchen Zhang, Hengxian Qu, Hengxian Qu, Dawei Chen, Dawei Chen, Xia Chen, Xia Chen, Yujun Huang, Yujun Huang, Ruixia Gu, Ruixia Gu

“…Our previous studies showed f-EPS production from S. thermophilus 937 was increased 2-fold in the presence of 15 mM of glutamate, isoleucine, and histidine in the chemically defined medium.MethodsIn this study, we used transcriptomics and qPCR to further explore the specific mechanism of the enhanced effect of 3 amino acids on the f-EPS biosynthesis of S. thermophilus 937.ResultsThe mRNA-seq analysis and targeted pathway analysis indicated that genes associated with histidine/valine/leucine/ isoleucine/phenylalanine/tyrosine/tryptophan synthesis, galactose metabolism, purine metabolism and quorum sensing in S. thermophilus 937 were significantly upregulated under increasing concentrations of histidine, isoleucine and glutamate in chemically defined medium (CDM). qPCR results showed that the significant upregulation of galactose metabolism- and nucleotide sugar synthesis-related genes was attributed to the increase in glutamate concentration, and glutamate could induce the expression of galR. …”
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The StbHLH47 transcription factor negatively regulates drought tolerance in potato (Solanum tuberosum L.) by Peijie Wang, Xiaojuan Wu, Nan Li, Hushuai Nie, Yu Ma, Juan Wu, Zhicheng Zhang, Yanhong Ma

“…Additionally, RT‒qPCR was used for fluorescence quantification of the expression of StNCED1 and StERD11, which are known for their drought resistance, and the results revealed that the expression levels were much lower in OE-StbHLH47 than in WT plants. Conclusion RNA-seq, RT‒qPCR, and physiological index analyses under drought conditions revealed that overexpression of the StbHLH47 gene increased the sensitivity of potato plants to drought stress, indicating that StbHLH47 negatively regulates drought tolerance in potato plants. …”
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间充质干细胞抑制MEK/ERK信号通路改善盲肠结扎穿孔所致急性肺损伤中肺泡Ⅱ型上皮细胞的凋亡

“…肺组织fas?bax/bcl-2及caspase3 mRNA扩增倍数CLP/Saline vs CLP/MSC p值分别为0.09?0.004及0.037,均 < 0.05,蛋白水平fas?…”
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The injury effect of osteopontin in sepsis-associated lung injury by Qian Wang, Zhicai Yu, Zhixin Song, Xue Lu, Zhu Li, Dandan Pi, Jing Li, Feng Xu

“…Mechanistic investigations in MH-s cells revealed that OPN-siRNA suppressed the LPS-induced macrophage inflammatory response by inhibiting caspase1-dependent classical pyroptosis signaling pathway. …”
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乳酸脱氢酶A在肾细胞癌中的表达及意义 by 邱文瀚, 廖定准, 盛义雨, 熊海云, 李骏

“…构建<italic>LDHA</italic>-shRNA重组质粒，转染至786-O以敲低<italic>LDHA</italic>表达，利用CCK-8、克隆形成实验及EdU染色法检测敲低<italic>LDHA</italic>表达对癌细胞增殖活性的影响。…”
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Cyclophilin–CD147 interaction enables SARS-CoV-2 infection of human monocytes and their activation via Toll-like receptors 7 and 8 by Gabor Tajti, Laura Gebetsberger, Gregor Pamlitschka, Katharina Aigner-Radakovics, Judith Leitner, Peter Steinberger, Hannes Stockinger, Anna Ohradanova-Repic

“…Secondly, upon viral uptake via the cyclophilin-CD147 interaction, that can be inhibited by specific CD147 blocking antibodies or competition with recombinant human cyclophilin A and B, SARS-CoV-2 RNA is recognized by TLR7/8 in endosomes, leading to upregulation of tumor necrosis factor (TNF), interleukin (IL)-1β and IL-6, comprising the core hyperinflammatory signature. …”
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