Biodesulfurization of high-sulfur oil from the Karazhanbas field of Kazakhstan with deep eutectic solvents by A.O. Akimbek, G.A. Jamalova, A.K. Yernazarova, G.K. Kaiyrmanova, B.K. Yelikbayev, M.C. Pagano, A.G. Zazybin, Kh. S. Rafikova

“…The research methodology was based on the application of the Koch methods to determine the total number of microorganisms; light microscopy – for the study of microbiological preparations; genetic identification of bacteria based on the analysis of the nucleotide sequence of a fragment of the 16S rRNA gene; synthesis of deep eutectic solvents; testing of isolated bacteria – for sensitivity to Cr (VI), for the ability of microorganisms to use hydrocarbons of high-sulfur oil, for activity in sulfur-containing crude oil, for determination of the mass fraction of sulfur. …”
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IL-33 Mediates Lung Inflammation by the IL-6-Type Cytokine Oncostatin M by Fernando Botelho, Anisha Dubey, Ehab A. Ayaub, Rex Park, Ashley Yip, Allison Humbles, Roland Kolbeck, Carl D. Richards

“…Adenoviral OSM (AdOSM) markedly induced IL-33 mRNA and protein levels in wild-type animals while IL-33 was undetectable in IL-33-/- animals. …”
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C2C12成肌细胞体外诱导分化为肌管的实验 by 陈永乐, 周光前, 邓宇斌, 智伟, 谢彗琪, 邓力, 杨志明, 王亚柱

“…③20 mL/L马血清诱导C 2C 12细胞向成熟肌细胞分化过程中，肌相关蛋白分子表达发生变化：结蛋白（desmin）、成肌分化抗原（MyoD）、生肌素（myogenin）和肌球蛋白（myosin）等表达逐渐增强（未刺激时分别约为49．6％ ± 1．1％、23．4％ ± 1．1％、4．8％ ± 1．6％和2．6％ ± 1．5％；第6天时分别提高为80．4％ ± 1．8％、85．4％ ± 1．1％、22．2％ ± 1．1％和26．0％ ± 1．6％；P<0．001）；desmin、MyoD和myogenin相应的mRNA分子也可以通过RT-PCR检测到。【结论】 ①低浓度的马血清能够有效刺激C 2C 12成肌细胞向成熟细胞分化，以20 mL/L浓度效果最好；②C 2C 12细胞是研究肌细胞发育分化的理想模板；③肌分化发育过程比较复杂，许多因素都可能起影响，研究设计要尽量标准化。…”
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Transcriptomics analysis reveals potential mechanisms underlying mitochondrial dysfunction and T cell exhaustion in astronauts’ blood cells in space by Maria Moreno-Villanueva, Maria Moreno-Villanueva, Luis E. Jimenez-Chavez, Luis E. Jimenez-Chavez, Stephanie Krieger, Liang-Hao Ding, Ye Zhang, Adriana Babiak-Vazquez, Mark Berres, Sandra Splinter, Kristen E. Pauken, Brian C. Schaefer, Brian E. Crucian, Honglu Wu

“…Transcriptomic analysis was performed in isolated peripheral blood mononuclear cells (PBMCs) using RNA-sequencing. Differentially expresses genes (DEG) in space were determined by comparing of the inflight to the preflight samples. …”
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High serum CA19-9 predicts severe cholecystitis in calculous cholecystitis patients by Shouwen Wang, Dachen Zhou, Wanjin Chen, Qi Guo, Liujin Hou, Ruolin Wu, Wei Wang, Muhammad Annus Khan, Muhammad Ahmad, Fan Huang, Meijuan Zheng, Guobin Wang, Hongchuan Zhao, Xiaoping Geng, Xiaojun Yu

“…Besides, the gallbladder specimens were collected for immunohistochemical staining and mRNA sequencing. Results Abdominal pain occur in more than 90% patients in high group, which is similar with that of normal group. …”
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Assembly of Genetically Engineered Ionizable Protein Nanocage-based Nanozymes for Intracellular Superoxide Scavenging by Qiqi Liu, Zhanxia Gao, Xiangyun Zhang, Qiannan Duan, Yue Zhang, Adam C. Midgley, Li Jiao, Ruming Liu, Mingsheng Zhu, Deling Kong, Jie Zhuang, Xinglu Huang

“…The in vivo protective effects of these ionizable cascade nanozymes against cardiac oxidative injury are demonstrated in female mouse models of cardiac ischemia-reperfusion (IR). RNA-sequencing analysis highlight the crucial role of these nanozymes in modulating superoxide anions-, hydrogen peroxide- and mitochondrial functions-relevant genes in IR injured cardiac tissue. …”
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Development of highly bioactive long-acting recombinant porcine FSH for batch production management of sows by Jiawei Zhang, Lei Shi, Xiaorong Zhong, Yunhe Bai, Jun Dou, Lu Zhang, Xiaochao Shi, Bingchun Wu, Zemin Tan, Lin Yan, Jinku Yu, Jingru Zhang, Guo Han, Jiansheng Xiong, Hua Zhang, Xingyuan Cao, Haoshu Luo

“…Through ovarian weight gain, superovulation and fertility testing assays, our results revealed that a single rpFSH-pFc treatment could effectively promote follicular development and maturation in vivo. Meanwhile, the mRNA expression levels of the target genes associated with follicular development, maturation and ovulation were significantly up-regulated after rpFSH-pFc treatment. …”
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Knockdown of Gfi1 increases BMSCs exosomal miR-150-3p to inhibit osteoblast ferroptosis in steroid-induced osteonecrosis of the femoral head through BTRC/Nrf2 axis by Liwen Zheng, Changjie Zhang, Lele Liao, Zhijie Hai, Xin Luo, Haoliang Xiao

“…To verify the targeting relationship between growth factor independence 1 (Gfi1) and the miR-150-3p promoter, as well as between miR-150-3p and beta-transducin repeat containing E3 ubiquitin protein ligase (BTRC), respectively, chromatin immunoprecipitation (ChIP), RNA immunoprecipitation (RIP), and dual luciferase assays were employed. …”
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A Multifunctional Cobalt‐Containing Implant for Treating Biofilm Infections and Promoting Osteointegration in Infected Bone Defects Through Macrophage‐Mediated Immunomodulation by Nongyang Yan, Hao Zhou, Penghe Jin, Tengfei Li, Qi Liu, Hao Ning, Zhixin Ma, Linfei Feng, Tao Jin, Youwen Deng, Zhengwei Wu

“…Additionally, the implants exhibit superior anti‐inflammatory and osseointegration properties. RNA sequencing analysis reveals the potential mechanism of Co2+ in regulating the polarization of macrophages toward the anti‐inflammatory M2 phenotype, which is crucial for creating an immune environment conducive to bone healing. …”
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Genetic Polymorphisms of Toll-like receptors 2 and 9 as Susceptibility Factors for the Development of Ankylosing Spondylitis and Psoriatic Arthritis by Camila F. Oliveira-Toré, Amarilis G. Moraes, Gabriela F. Martinez, Janisleya S. F. Neves, Luciana C. Macedo, Marco A. Rocha-Loures, Marília G. Quirino, Hugo V. Alves, Ana M. Sell, Jeane E. L. Visentainer

“…Polymorphisms in TLR genes that lead to changes in these receptors or that interfere with the transcription rates of mRNA TLR may be involved in the chronic inflammatory immune response observed in SpA. …”
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Immune infiltration landscape and potential drug-targeted implications for hepatocellular carcinoma with ‘progression/hyper-progression’ recurrence by Jing-Xuan Xu, Yue-Xiang Su, Yuan-Yuan Chen, Yi-Yue Huang, Zu-Shun Chen, Yu-Chong Peng, Lu-Nan Qi

“…Virtual docking was used to identify potential therapeutic targets, and the results were verified using single-nuclei RNA sequencing and histological analysis.Results ITM was closely related to type III–IV recurrence and exhibited immunotherapy potential. …”
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Cancer-specific innate and adaptive immune rewiring drives resistance to PD-1 blockade in classic Hodgkin lymphoma by Julia Paczkowska, Ming Tang, Kyle T. Wright, Li Song, Kelsey Luu, Vignesh Shanmugam, Emma L. Welsh, Jason L. Weirather, Naomi Besson, Harrison Olszewski, Billie A. Porter, Kathleen L. Pfaff, Robert A. Redd, Fathima Zumla Cader, Elisa Mandato, Jing Ouyang, Eleonora Calabretta, Gali Bai, Lee N. Lawton, Philippe Armand, Scott J. Rodig, Xiaole Shirley Liu, Margaret A. Shipp

“…Here, we utilize single-cell RNA sequencing and spatial analysis to define shared circulating and microenvironmental features of the immune response to PD-1 blockade in cHL. …”
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Germline variants of the genes involved in NF-kB activation are associated with the risk of COPD and lung cancer development by Baranasic Jurica, Niazi Yasmeen, Chattopadhyay Subhayan, Rumora Lada, Ćorak Lorna, Dugac Andrea Vukić, Jakopović Marko, Samaržija Miroslav, Försti Asta, Knežević Jelena

“…Functional studies conducted on COPD patients showed that RNA expression of IL2RA, IFNγ and related proinflammatory cytokines in blood serum did not correlate with a specific genotype. …”
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Targeting unique ligand binding domain structural features downregulates DKK1 in Y537S ESR1 mutant breast cancer cells by K. S. Young, G. R. Hancock, E. C. Fink, A. Zigrossi, B. Flowers, D. A. Cooper, V. T. Nguyen, M. C. Martinez, K. S. Mon, M. Bosland, D. R. Zak, A. P. Runde, M. N. Sharifi, I. Kastrati, D. D. L. Minh, S. Kregel, Sean W. Fanning

“…Here, we use a comprehensive suite of structural-biochemical, in vitro, and in vivo approaches to better T6I-29’s activities in breast cancer cells harboring Y537S ERα. RNA sequencing in cells treated with T6I-29 reveals a neomorphic downregulation of DKK1, a secreted glycoprotein known to play oncogenic roles in other cancers. …”
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Comparative Analysis of Meat Quality and Hindgut Microbiota of Cultured and Wild Bighead Carp (<i>Hypophthalmichthys nobilis</i>, Richardson 1845) from the Yangtze River Area by Abdullateef Mukhtar Muhammad, Chang Yang, Bo Liu, Cunxin Sun, Linghong Miao, Xiaochuan Zheng, Liangkun Pan, Dong Xia, Qun-Lan Zhou

“…Moreover, the hindgut microbiota was analyzed using 16S rRNA high-throughput sequencing. The alpha and beta diversity differences between the cultured and wild groups were significant. …”
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Deficiency of purinergic P2Y2 receptor impairs the recovery after renal ischemia-reperfusion injury and accelerates renal fibrosis and tubular senescence in mice by Kyuho Jeong, Jihyun Je, Theodomir Dusabimana, Jacques Karekezi, Tatang Aldi Nugroho, Edvard Ntambara Ndahigwa, Hyun Joon Kim, Seung Pil Yun, Hye Jung Kim, Hwajin Kim, Sang Won Park

“…Proximal tubular cells treated with JunB siRNA showed a reduced expression of fibrogenic mediators and proinflammatory cytokines, consistent with the mice treated with MRS2768, a P2Y2 agonist that downregulated JunB levels. …”
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Retinoic Acid Signal Negatively Regulates Osteo/Odontogenic Differentiation of Dental Pulp Stem Cells by Jiangyi Wang, Guoqing Li, Lei Hu, Fei Yan, Bin Zhao, Xiaoshan Wu, Chunmei Zhang, Jinsong Wang, Juan Du, Songlin Wang

“…Activation of RA signal in vitro inhibited ALP activities and mineralization of human DPSCs and decreased the mRNA expression of ALP, osteocalcin, osteopontin, and a transcription factor, osterix. …”
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Deciphering the Transcriptional Metabolic Profile of Adipose-Derived Stem Cells During Osteogenic Differentiation and Epigenetic Drug Treatment by Giulia Gerini, Alice Traversa, Fabrizio Cece, Matteo Cassandri, Paola Pontecorvi, Simona Camero, Giulia Nannini, Enrico Romano, Francesco Marampon, Mary Anna Venneri, Simona Ceccarelli, Antonio Angeloni, Amedeo Amedei, Cinzia Marchese, Francesca Megiorni

“…In this study, we employed the NanoString nCounter technology, an advanced multiplexed digital counting method of RNA molecules, to comprehensively characterize differentially expressed transcripts involved in metabolic pathways at distinct time points in osteogenically differentiating ASCs treated with or without the pan-DNMT inhibitor RG108. …”
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Pan-cancer characterization of m6A-mediated regulation of T cell exhaustion dynamics and clinical relevancies in human cancers by Weiping Ji, Ye Fang, Liwei Chen, Yitong Zheng, Yifei Pei, Changqiu Mei, Meng Zhou

“…Here, we conducted a pan-cancer analysis integrating multi-omics data from cell lines, single-cell RNA sequencing, and pan-cancer and immunotherapy datasets to explore the dynamic interplay between m6A modification and TEX. …”
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Association of breast milk microbiota and metabolites with neonatal jaundice by TianYu Liu, TianYu Liu, Yanhan Yuan, Jinying Wei, Jinying Wei, Jiayi Chen, Feng Zhang, Feng Zhang, Juanjuan Chen, Jinping Zhang

“…Breast milk samples were collected from mothers in both groups on postnatal day 4 and analyzed for microbiota and small-molecule metabolites using 16S rRNA gene sequencing and an liquid chromatography-tandem mass spectrometry techniques.ResultsA total of 104 mother-child pairs were included in the study, of which 51 pairs were in the healthy control group and the other 53 pairs were in the jaundice group. …”
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