Direct and indirect effects of land use on microbiomes of trap-nesting solitary bee larvae and nests by Birte Peters, Birte Peters, Birte Peters, Sara Diana Leonhardt, Michael Schloter, Alexander Keller

“…However, the drivers and dynamics of healthy microbiome in solitary bees are still poorly understood, especially regarding the direct and indirect effects of land use on the diversity and composition of these microbial communities.MethodsWe examined bacterial communities in the offspring and nest materials of the Megachilid trap-nesting solitary bee, Osmia bicornis, along a gradient of land use intensification by 16S rRNA gene metabarcoding. Given that landscape composition, climatic conditions, and food resources are known to influence microbial compositions in solitary bee species, we hypothesized that land use changes would alter resources available for food and nest material collection and thereby affecting the microbiomes in offspring and their nest environments. …”
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Identification of novel cytoskeleton protein involved in spermatogenic cells and sertoli cells of non-obstructive azoospermia based on microarray and bioinformatics analysis by Danial Hashemi Karoii, Hossein Azizi, Maryam Darvari, Ali Qorbanee, Dawan Jamal Hawezy

“…In the study of MF, U3 snoRNA binding, and cadherin binding were significantly associated with common DEGs. …”
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miR-424-5p Promotes Proliferation, Migration and Invasion of Colorectal Cancer Cells via the Targeting TXNIP/Hippo Axi by Zhang F, Zhu KL, Chen R, Su F

“…Mechanically, TXNIP overexpression significantly inhibited YAP/TAZ transcriptional activity, and the highly expressed miR-424-5p in CRC targeted TXNIP mRNA.Conclusion: The study clarify a novel miR-424-5p/TXNIP/Hippo signaling pathway that facilitated CRC cells proliferation, migration and invasion. …”
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Effects of Different River Crab <i>Eriocheir sinensis</i> Polyculture Practices on Bacterial, Fungal and Protist Communities in Pond Water by Yun Bao, Bing Li, Rui Jia, Linjun Zhou, Yiran Hou, Jian Zhu

“…We analyzed the water bacterial, fungal, and protist communities in the PC and MC groups using 16S, ITS, and 18S ribosomal RNA high-throughput sequencing. We found that the PC group obviously increased the diversity of microbial communities and altered their composition. …”
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Biocontrol Potential of Rhizospheric Bacillus Strains Against <i>Sclerotinia minor</i> Jagger Causing Lettuce Drop by Lihui Xu, Qinghua Shang, Mogens Nicolaisen, Rong Zeng, Shigang Gao, Ping Gao, Zhiwei Song, Fuming Dai, Jingze Zhang

“…Bioactivity experiments showed that Bac20 had higher inhibitory activity against <i>S. minor</i> Jagger than Bac45. Based on 16S rRNA sequences and phylogenetic analysis of a combination of sequences from <i>gyrA</i>, <i>rpoB</i>, <i>purH</i>, <i>polC</i>, and <i>groEL</i>, Bac20 and Bac45 were identified as <i>Bacillus velezensis</i> and <i>Bacillus subtilis</i>, respectively. …”
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Therapeutic Efficacy of <i>Lavandula dentata</i>’s Oil and Ethanol Extract in Regulation of the Neuroinflammation, Histopathological Alterations, Oxidative Stress, and Restoring Ba... by Aziza Antar, Eman S. Abdel-Rehiem, Areej A. Al-Khalaf, Abdelaziz S. A. Abuelsaad, Mohamed Abdel-Gabbar, Gaber M. G. Shehab, Ayman M. Abdel-Aziz

“…Numerous electrolyte levels, monoamine levels, neurotransmitter levels, and the mRNA expression of specific gate channel subtypes were evaluated in homogenate brain tissue. …”
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Machine learning models reveal microbial signatures in healthy human tissues, challenging the sterility of human organs by Anargyros Skoulakis, Anargyros Skoulakis, Giorgos Skoufos, Giorgos Skoufos, Armen Ovsepian, Armen Ovsepian, Artemis G. Hatzigeorgiou, Artemis G. Hatzigeorgiou

“…Machine learning (ML) models were developed to classify each tissue type based solely on microbial profiles, with the identification of tissue-specific microbial signatures suggesting the presence of distinct microbial communities inside tissues.MethodsWe analyzed 13,871 normal RNA-seq samples from 28 tissues obtained from the GTEx consortium. …”
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Patchoulene Epoxide Isolated from Patchouli Oil Suppresses Acute Inflammation through Inhibition of NF-κB and Downregulation of COX-2/iNOS by Jia-Li Liang, Jia-Zhen Wu, Yu-Hong Liu, Zhen-Biao Zhang, Qi-Duan Wu, Han-Bin Chen, Yan-Feng Huang, Yao-Xing Dou, Jiang-Tao Zhou, Zi-Ren Su, Janis Ya-Xian Zhan

“…PAO was also shown to significantly downregulate the protein and mRNA expressions of cyclooxygenase-2 (COX-2) and inducible nitric-oxide synthase (iNOS). …”
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大麻素1型受体在实验性自身免疫性脑脊髓炎小鼠中的 动态表达及其作用

“…EAE 和特异性CB1R抑制剂(SR141716A,SR1)干预组小鼠的神经功能缺损症状和体质量变化;用real time PCR检测CB1R mRNA表达;Western blot和荧光免疫组织化学法检测CB1R蛋白表达;用ELISA法检测细胞因子IL-1β?…”
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Identification of Breast Cancer Biomarkers by Constructing Protein-Protein Interaction and miRNAs-mRNAs Networks by Azizeh Asadzadeh, Bita Behboodian, Parsa Bernoos, Masoumeh Shojaei Barjouei

“…GEO2R was used to obtain differentially expressed mRNA (DEMs) and miRNAs (DEMIs) based on |logFC|>2 and adjusted P-value <0.05. …”
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Impact of copper stress in the intestinal barriers and gut microbiota of Chinese stripe-necked turtle (Mauremys sinensis) by Zeeshan Ali, Ijaz Khan, Muhammad Shahid Iqbal, Haitao Shi, Li Ding, Meiling Hong

“…Copper stress caused intestinal damage, characterized by shortened villi, inflammatory cell infiltration, and reduced epithelial layer thickness, as well as decreased acidic mucins, increased villi edema and inflammation. The mRNA expression level of bacteriostatic enzymes significantly reduced. …”
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Polydatin protects against DSS-induced ulcerative colitis via Nrf2/Slc7a11/Gpx4-dependent inhibition of ferroptosis signalling activation by Shimin Zheng, Jianbin Yin, Bingbing Wang, Qiujuan Ye, Jialuo Huang, Xinzhi Liang, Junfeng Wu, Hui Yue, Ting Zhang

“…In this study, we presented evidence that PD confers protection against dextran sodium sulfate (DSS)-induced ulcerative colitis.MethodsThe protective effect of PD on colitis was examined in cultured caco-2 cells and DSS-induced colitis mouse model. Bulk RNA sequencing and differential gene expression analysis were used to investigate the protective mechanism of PD on DSS-induced colitis. …”
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Long-term maintenance of patient-specific characteristics in tumoroids from six cancer indications by Colin D. Paul, Chris Yankaskas, Pradip Shahi Thakuri, Brittany Balhouse, Shyanne Salen, Amber Bullock, Sylvia Beam, Anthony Chatman, Sybelle Djikeng, Xiaoyu Jenny Yang, Garrett Wong, Isha Dey, Spencer Holmes, Abigail Dockey, Lindsay Bailey-Steinitz, Lina Zheng, Weizhong Li, Vivek Chandra, Jakhan Nguyen, Jason Sharp, Erik Willems, Mark Kennedy, Matthew R. Dallas, David Kuninger

“…Newly derived tumoroid lines (n = 20) were analyzed by targeted genomic profiling and RNA sequencing and were representative of tumor tissue samples. …”
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TLR4 Inhibition Attenuated LPS‐Induced Proinflammatory Signaling and Cytokine Release in Mouse Hearts and Cardiomyocytes by Christine W. Wiger, Trine Ranheim, Henriette Arnesen, Jarle Vaage, Søren E. Pischke, Arne Yndestad, Kåre‐Olav Stensløkken, May‐Kristin Torp

“…Moreover, TLR4 inhibition reduced LPS‐induced mRNA expression and release of IL‐6 in primary adult cardiomyocytes. …”
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A cell-based model to study mechanisms of endothelial-dependent thrombin generation in response to inflammation and its modulation by hydroxychloroquine by Deepa J. Arachchillage, Golzar Mobayen, Mike Laffan, Anna M. Randi, Josefin Ahnström, Charis Pericleous

“…The expression of key coagulant and inflammatory regulators was measured at the mRNA level. Tissue factor (TF) protein levels were further assessed by flow cytometry. …”
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FMT reduces systemic inflammatory response in severe acute pancreatitis by increasing the abundance of intestinal Bifidobacteria and fecal bacteria by Yanning Mao, Yandong Huang, Weiwei Zhang, Huiping Liang, Fengming Liu, Qi Luo, Chunqin Xu, Yi Qin, Jiawen Liu, Shaobo Tang, Huaying Liu, Xiaolong Ge

“…In this study, fecal samples from healthy, control, and FMT-treated groups were analyzed using 16S rRNA sequencing to assess microbiome abundance and diversity. …”
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Establishment of the first marine mollusk cell line from scallop (Chlamys farreri) trochophore by Zhenkui Qin, Aichang Ji, Meng Yan, Danwen Liu, Xixi Li, Xiaoli Hu, Zhifeng Zhang

“…The origin of the CfT cell line was verified as C. farreri using internal transcribed spacer (ITS) and 18 s rRNA gene amplification, genomic in situ hybridization (GISH), and karyotype analysis. …”
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Construction of fluorescent transgenic zebrafish Tg(amh:mCherry) and tracer analysis of its gonadal development by WU Jing, XU Yue, LING Shiying, SONG Huaidong, QIAO Jie, DONG Mei

“…The recombinant transgenic expression vector and mRNA of Tol2 transposase were co-microinjected into zebrafish embryos, and fluorescent zebrafish were selected by using fluorescence microscope to screen for a stable inherited transgenic zebrafish line Tg(amh:mCherry). …”
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蚊虫细胞色素P450 基因系列研究 by 黄炯烈, 周国理, 吴　瑜, 葛春喜, 王　玲

“…GenBank accession number 分别 为AF283836-AF283838(全长cDNA 序列)和AF284782-AF284783(非全长cDNA 序列), 被国际P450 命名委员会正式命名为 CYP 6N3v1-v3(全长cDNA 序列)和CYP 6N3v4 、CYP 6N4v1-v6, 并证实这些新基因同时存在于白纹伊蚊溴氰菊酯抗性株及 敏感株中;③运用基因步移方法从白纹伊蚊溴氰菊酯抗性株中扩增出CYP 6N3 上游3 076 bp 调控序列;④对白纹伊蚊 CYP 6N3、CYP 6N4 非翻译区序列功能分析显示:昆虫CYP450 与其它真核m RNA 翻译起始及终止机制相似, 但具有多态性 的特点;⑤对白纹伊蚊CYP 6N3 上游启动子区序列特征与功能分析显示:蚊虫中CYP450 转录起始存在着复杂性;⑥证实蚊 虫中CYP 6 存在多样性, 并分析讨论了此种多样性形成的可能机制;⑦分析讨论了蚊虫细胞色素P450 CYP 6 家族分子进化 机制;⑧阐明了下一步研究的目标。…”
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Induced mesenchymal stem cells generated from periodontal ligament fibroblast for regenerative therapy by Hemanathan Vembuli, Sheeja Rajasingh, Patrick Nabholz, Jefferson Guenther, Brian R. Morrow, Margaret M. Taylor, Marziyeh Aghazadeh, Vinoth Sigamani, Johnson Rajasingh, Johnson Rajasingh, Johnson Rajasingh

“…In this study, we generated induced pluripotent stem cells (iPSCs) using a safe, nonviral and mRNA-based approach from human periodontal ligament fibroblasts (PDLF), an easily accessible cell source. …”
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