Anti-Inflammatory and Anti-Migratory Effects of Morin on Non-Small-Cell Lung Cancer Metastasis via Inhibition of NLRP3/MAPK Signaling Pathway by Punnida Arjsri, Kamonwan Srisawad, Sonthaya Umsumarng, Pilaiporn Thippraphan, Songyot Anuchapreeda, Pornngarm Dejkriengkraikul

“…Morin downregulated invasive proteins (MMP-2, MMP-9, u-PAR, u-PA, MT1-MMP) and EMT markers (fibronectin, N-cadherin, vimentin) (<i>p</i> < 0.01) while also reducing the mRNA levels of NLRP3, IL-1β, IL-18, and IL-6. Mechanistic investigations revealed that morin suppressed NLRP3 inflammasome activity and inactivated MAPK pathways. …”
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Portable UV-C device to treat high flow of infectious aerosols generated during clinical respiratory care by Richard Vincent, David Rapoport, Priti Balchandani, Joseph Borrello, Michael Schotsaert, Robert Karlicek, Gabriel Laghlali, Prajakta Warang, Seokchan Park, Gagandeep Singh, Isabella Morgan, James Paredes, Raveen Rathnasinghe, Jacob Wolf, Adolfo García-Sastre

“…Testing was with nebulized A/PR/8/34 (H1N1) influenza virus. RNA extraction and qRT-PCR showed virus transited through the prototype. …”
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NAD⁺激活SIRT3/IDH2信号通路改善高血压血管内皮功能障碍 by 丘雨旻, 陈曦, 张建宁, 刘张弛, 朱秋霞, 张美信, 陶军, 吴杏

“…采用迁移实验和划痕实验评估体外内皮细胞功能；采用免疫荧光染色法检测mtROS水平；采用RT-PCR检测mtDNA、SIRT3、异柠檬酸脱氢酶2（IDH2）的mRNA表达；采用酶联免疫吸附法检测8-羟基-2'-脱氧鸟苷的水平；采用Western blot法检测p-eNOS、eNOS、SIRT3、IDH2蛋白表达水平。…”
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Three local plants adapt to ecological restoration of abandoned lead-zinc mines through assembly of rhizosphere bacterial communities by Wei Gao, Shuyi Chen, Xin Yu, Sumin Chen, Caijing Wan, Ying Wang, Peng Wu, Qiang Li

“…IntroductionThe plant restoration and ecological restoration of lead-zinc mines are very important.MethodsIn this study, we used three local plants to carry out ecological restoration of abandoned lead–zinc mining areas and detected the adaptive mechanisms of soil bacterial diversity and function during the ecological restoration of lead–zinc mines through 16S rRNA sequencing.ResultsThe results revealed that lead-zinc mining significantly reduced the soil bacterial diversity, including the Shannon, Simpson, and observed species indices, whereas the planting of the three ecological restoration plants restored the soil microbial diversity to a certain extent, leading to increases in the Shannon index and Observed species indices. …”
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Discovery of mutated oncodriver genes associated with glioblastoma originated from stem cells of subventricular zone through whole exome sequence profile analysis, and drug repurpo... by Arnob Sarker, Burhan Uddin, Reaz Ahmmed, Sabkat Mahmud, Alvira Ajadee, Md. Al Amin Pappu, Md. Abdul Aziz, Md. Nurul Haque Mollah

“…Then, filtering by differential co-expression analysis based on independent RNA-Seq profiles of CGGA database revealed 10 genes as dysregulated oncodriver genes. …”
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Synthetic inhibition of SREBP2 and the mevalonate pathway blocks rhabdomyosarcoma tumor growth in vitro and in vivo and promotes chemosensitization by Silvia Codenotti, Michela Asperti, Maura Poli, Luisa Lorenzi, Alberto Pietrantoni, Matteo Cassandri, Francesco Marampon, Alessandro Fanzani

“…Methods: In silico analyses of RNA datasets were performed to correlate MVP with RMS patient survival. …”
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GRK6 palmitoylation dictates triple-negative breast cancer metastasis via recruiting the β-Arrestin 2/MAPKs/NF-κB signaling axis by Wen-Ke Wang, Hui-Yu Lin, Che-Hsuan Lin, Hsun-Hua Lee, Yen-Lin Chen, Yu-Hsien Kent Lin, Hui-Wen Chiu, Shry-Ming Sheen-Chen, Yuan-Feng Lin

“…Methods Bioinformatics analysis was conducted to evaluate the mRNA/protein expression and prognostic significance of G protein–coupled receptor kinase 6 (GRK6) in BC subtypes. …”
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The active roles of Rhodotorula mucilaginosa ZTHY2 in regulating antioxidant capacity and immune function of Leizhou black ducks by Jiang Wu, Yingxin Hu, Namula Zhao, Wei Yang, Zhibao Chen, Zhibao Chen

“…Moreover, ZTHY2 modulated the mRNA expression profiles of these cytokines in the thymus, spleen, and bursa, thereby sustaining the balance of immune dynamics. …”
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Comparison of phenotypic and genetic traits of ESBL-producing UPEC strains causing recurrent or single episode UTI in postmenopausal women by Michelle Kalu, Peter Jorth, Annie Wong-Beringer

“…Presence of hlyA, ompT, and fimH genes were confirmed by colony PCR, and qRT-PCR was performed using extracted RNA from a subset of 18 strains (12 recurrent; 6 single episode) grown in Luria-Bertani media and isolated from infected BECs to characterize gene expression. …”
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Transcriptome Sequencing Reveals Regulatory Mechanisms of Taxol Synthesis in Taxus wallichiana var. Mairei by Tao Wang, Yiming Chen, Weibing Zhuang, Fengjiao Zhang, Xiaochun Shu, Zhong Wang, Qing Yang

“…To investigate the specific regulatory mechanisms of taxol synthesis in Taxus wallichiana var. mairei, RNA-seq was conducted to reveal the differences in transcriptional levels between wild type (WT) and “Jinxishan” (JXS), a cultivar selected from a population of Taxus mairei that shows about 3-fold higher taxol content in the needles than WT. …”
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PTEN suppresses renal cell carcinoma proliferation and migration via inhibition of the PI3K/AKT pathway by Xu Xu, Yuan-Yue Tang, Xiaohong Liang, Wen Luo, Dong-Mei Jiang, Jie Chen

“…Results RT-qPCR results showed that PTEN expression was significantly increased in RCC tumor tissues compared to normal tissues (p < 0.01). However, PTEN mRNA levels were significantly reduced in 786-O cells compared to HK-2 cells (p < 0.01). …”
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SHP2 promotes the epithelial-mesenchymal transition in triple negative breast cancer cells by regulating β-catenin by Shihan Qian, Jingjing Zhu, Qing Han, Huang Cheng, Huaibin Zhou

“…Furthermore, we knocked down SHP2 expression via RNA interference in three TNBC cell lines. To assess the impact of SHP2 on invasion and migration, we conducted transwell assays and wound healing experiments. …”
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Osteoarticular Infection in Three Young Thoroughbred Horses Caused by a Novel Gram Negative Cocco-Bacillus by Bernard J. Hudson, Catherine Chicken, Anna Blishen, Kristen H. Todhunter, Angela P. Begg, Leonie Chan, Thomas Karagiannis, Benjamin Raymond, Daniel Bogema, Angus R. Adkins, Christopher B. O’Sullivan, Brendon A. O’Rourke, Piklu Roy Chowdhury, Steven P. Djordjevic, Ian G. Charles, Andrew Edgar, Katerina Mitsakos

“…The pattern of OAI resembled that reported with Kingella infection in humans. Analysis by 16S rRNA PCR enabled construction of a phylogenetic tree that placed the isolates closer to Simonsiella and Alysiella species, rather than Kingella species. …”
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Comprehensive profiling of gut bacterial communities in hybrid red tilapia with and without streptococcosis by Pathamawan Amthungphong, Natthaphong Sombuttra, Triwit Rattanarojpong, Kanthida Kusonmano, Saengchan Senapin, Satid Chatchaiphan, Watanalai Panbangred, Jirayut Euanorasetr

“…The infected streptococcal isolates were confirmed by 16S rRNA sequencing. The 16S amplicon was sequenced in gut microbiome samples from healthy and diseased groups to analyze the bacterial composition profiles associated with red tilapia with and without streptococcosis. …”
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Protective Effects of Baicalein on Lipopolysaccharide-Induced AR42J PACs through Attenuation of Both Inflammation and Pyroptosis via Downregulation of miR-224-5p/PARP1 by Ming-Wei Liu, Chun-Hai Zhang, Shou-Hong Ma, De-Qiong Zhang, Li-Qiong Jiang, Yang Tan

“…Flow cytometry was applied to evaluate the apoptotic rates of AR42J PACs. The RNA and protein expression of miR-224-5p, poly ADP-ribose polymerase-1 (PARP1), nuclear transcription factor-κB65 (NF-κB65), phospho-kappa B alpha(p-IκB-α), interleukin(IL)-18R, NOD-like receptor thermal protein domain-associated protein 3 (NLRP3), gasdermin D (GSDMD), apoptosis-associated speck-like protein containing a CARD (ASC), and caspase-1 was detected based on the WB and RT-PCR assays. …”
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Clofarabine induces tumor cell apoptosis, GSDME-related pyroptosis, and CD8+ T-cell antitumor activity via the non-canonical P53/STING pathway by Jing Chen, Jie Wu, Xiang Chen, Qian Tao, Nian Liu, Cong Peng, Can Lu, Qiuqiu Li

“…The molecular mechanisms of Clo-induced tumor cell death were analyzed using western blotting, ELISA, reverse transcription-PCR, immunofluorescence, co-immunoprecipitation (CO-IP), short hairpin RNA, co-culture, chromatin immunoprecipitation, and flow cytometry. …”
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Changes in Intestinal Microbiota Are Associated with Islet Function in a Mouse Model of Dietary Vitamin A Deficiency by Yunting Zhou, Junming Zhou, Yumin Zhang, Jun Tang, Bo Sun, Wei Xu, Xiaohang Wang, Yang Chen, Zilin Sun

“…VAD diet-driven changes in intestinal microbiota followed the inflammatory response with increased intestinal permeability and higher mRNA expression of intestinal inflammatory cytokines through nuclear factor-κB signaling pathway activation. …”
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Exploring the Mechanism of <i>Clostridium autoethanogenum</i> Protein for Broiler Growth Based on Gut Microbiota and Serum Metabolomics by Chunqiao Shan, Yan Liu, Sisi Liu, Chuang Li, Chaoxin Ma, Hongmin Yu, Juan Li, Guotuo Jiang, Jing Tian

“…The present study used 16S rRNA gene sequencing and serum metabolomics to investigate the effect of CAP on the cecal microflora structure and serum metabolites in 42-day-old broiler chickens. …”
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DNA损伤修复基因<italic>FANCI</italic>与肝癌预后和免疫浸润相关性 by 游莹, 梅美华, 谭宁歆, 陈怡丽, 池沛冬, 何晓顺, 黄俊琪

“…方法在本研究中，使用TCGA、GTEx、TIMER2.0及qRT-PCR法、蛋白质印迹法和免疫组织化学法研究FANCI在肝癌中的表达及其与不同临床分期之间的相关性；使用Kaplan-Meier Plotter数据库分析FANCI与肝癌预后的关系；使用TISIDB数据库探索FANCI与肝癌免疫细胞浸润及免疫检查点的关系；使用STRING数据库探索FANCI相结合的蛋白；使用TCGA及GTEx数据库进行GO及KEGG富集分析；通过细胞实验探索了FANCI在肝癌中的作用。结果与正常组织相比，肝癌组织FANCI的mRNA和蛋白表达水平上调（<italic>P</italic>&lt;0.001）；高表达FANCI的肝癌患者预后较差（<italic>P</italic>&lt;0.001）；肝癌组织中FANCI的表达与组织活化的CD4⁺T细胞数目、Th2细胞数目及免疫检查点基因的表达呈正相关，且FANCI高表达组的B细胞及巨噬细胞浸润水平明显较低（<italic>P</italic>&lt;0.01）；GO及KEGG富集分析显示FANCI相关基因富集在氨基酸跨膜转运体活性等多种生物过程中；细胞实验发现敲低FANCI后，肝癌的增殖、侵袭及迁移能力下降（<italic>P</italic>&lt;0.05）。…”
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Identification of cepharanthine as an effective inhibitor of African swine fever virus replication by Chuanxiang Qi, Jiyoung Lee, Yongqiang Zhang, Huan Chen, Jiaxuan Lv, Zhenzhong Wang, Jinming Li, Xiaodong Wu, Yong-Sam Jung, Zhiliang Wang, Yingjuan Qian

“…Disrupting EHD2 with small interfering RNA promoted ASFV entry into clathrin-positive vesicles. …”
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