Preclinical optimization of a GPC2-targeting CAR T-cell therapy for neuroblastoma by Nan Li, Haiying Qin, Rosa Nguyen, Mitchell Ho, Ming Sun, Eytan Ruppin, Michael C Kelly, Yingying Cao, Reona Okada, Jeyshka M Reyes-González, Hannah G Stack, Charlie Seibert, Carol J Thiele

“…Mechanistic studies were performed using single-cell RNA-sequencing.Results Applying stringent in vitro assays and orthotopic in vivo NB models, we demonstrated that our single-chain variable fragment, CT3, integrated into a CAR vector with a CD28 hinge, CD28 transmembrane, and 4-1BB co-stimulatory domain (CT3.28H.BBζ) elicits the best preclinical anti-NB activity compared with other tested CAR constructs. …”
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The total extract of Abelmoschus manihot (L.) medic flowers (TEA) mediated Nrf2-TFAM signalling to regulate mitochondrial antioxidant mechanism by Ying Song, Xinyi Zhu, Beibei Wang, Qisong Li, Biwei Song

“…RT-PCR was used to detect mRNA and mtDNA expression. The expression of the target protein was detected by Western blotting and immunofluorescence. …”
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Overexpressing the ClpC AAA+ unfoldase accelerates developmental cycle progression in Chlamydia trachomatis by Aaron A. Jensen, Saba Firdous, Lei Lei, Derek J. Fisher, Scot P. Ouellette

“…By analyzing the global transcriptional response to ClpC overexpression using RNA sequencing, we observed a shift to earlier expression of canonical late developmental cycle genes and other EB-associated genes. …”
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Low impact of Zostera marina meadows on sediment and water microbiota under brackish conditions by Daniel P. R. Herlemann, Luis F. Delgado, David J. Riedinger, Víctor Fernández-Juárez, Anders F. Andersson, Christian Pansch, Lasse Riemann, Mia M. Bengtsson, Greta Gyraitė, Marija Kataržytė, Veljo Kisand, Sandra Kube, Georg Martin, Kasia Piwosz, Marcin Rakowski, Matthias Labrenz

“…To better understand the impact of the salty divide on the interaction between Z. marina and the surrounding sediment and water microbiota, we investigated the effects of Z. marina meadows on the surrounding microbiota across a salinity range of 6–15 in the Baltic Sea during the summer using 16S and 18S rRNA gene amplicon sequencing. Results Salinity was the most important factor for structuring the microbiota within both water and sediment. …”
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Seahorse nanos3 plays essential roles in germ cell development in the absence of nanos2 by Lingzhen Chen, Lu Tang, Qiang Lin, Yanhong Zhang

“…Nanos, which encodes conserved zinc-finger RNA-binding proteins, play essential roles for germline development in both vertebrates and invertebrates. …”
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Developmental deficits, synapse and dendritic abnormalities in a Clcn4 KO autism mice model: endophenotypic target for ASD by Seong Mi Lee, Yura Choi, Dayeon Kim, Ha Jin Jeong, Young Ho Do, Sohee Jung, Bomee Lee, Hyung Jun Choi, Suhyeon Kim, Jung-Min Oh, Songhee Jeon, Jinju Han, Yeni Kim

“…Surprisingly, these symptoms were improved by Risperidone treatment, a drug commonly used to treat ASD. RNA sequencing data from mouse neural progenitor cells (mNPCs) showed that the genes regulating trans-synaptic signaling, transmembrane transport, and neuronal projection development were significantly decreased in Clcn4 knockdown (KD) cells compared to wild type (WT). …”
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Mural cell dysfunction contributes to diastolic heart failure by promoting endothelial dysfunction and vessel remodelling by Mandy O. J. Grootaert, Alessandra Pasut, Jana Raman, Steven J. Simmonds, Bram Callewaert, Ümare Col, Mieke Dewerchin, Peter Carmeliet, Stephane Heymans, Elizabeth A. V. Jones

“…We combined single-cell RNA sequencing, NichetNet analysis and histology to determine the role of mural cell dysfunction in HFpEF. …”
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Sir2 and Fun30 regulate ribosomal DNA replication timing via MCM helicase positioning and nucleosome occupancy by Carmina Lichauco, Eric J Foss, Tonibelle Gatbonton-Schwager, Nelson F Athow, Brandon Lofts, Robin Acob, Erin Taylor, James J Marquez, Uyen Lao, Shawna Miles, Antonio Bedalov

“…We have previously reported that in the absence of SIR2, a de-repressed RNA PolII repositions MCM replicative helicases from their loading site at the ribosomal origin, where they abut well-positioned, high-occupancy nucleosomes, to an adjacent region with lower nucleosome occupancy. …”
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Identification of SETD4 as an Onco‐Immunological Biomarker Encompassing the Tumor Microenvironment, Prognoses, and Therapeutic Responses in Various Human Cancers by Yuyun Zhong, Ruiqi Wang, Zijie Huang, Zhaoting Hu, Bin Peng, Bin Chen, Liyue Sun

“…The analysis revealed that the mRNA level of SETD4 was modulated by promoter methylation, and patients with lower methylation levels showed shorter survival times. …”
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TAp63γ is the primary isoform of TP63 for tumor suppression but not development by Xinbin Chen, Wenqiang Sun, Xiangmudong Kong, Xin Ming, Yanhong Zhang, Wensheng Yan, Shakur Mohibi, Mingyi Chen, Keith Mitchell, Jin Zhang

“…Moreover, mice deficient in p63γ are prone to chronic inflammation in multiple organs and liver steatosis potentially via altered lipid metabolism. Single-cell RNA-seq revealed that loss of p63γ increases the expression of SCD1, a rate-limiting enzyme for synthesis of monounsaturated fatty acids, leading to altered lipid homeostasis. …”
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Computational network analysis of two popular skin cancers provides insights into the molecular mechanisms and reveals common therapeutic targets by Md Sujan Mahmud, Bikash Kumar Paul, Md. Rakibul Hasan, K.M. Tanjida Islam, Imran Mahmud, Shahin Mahmud

“…PPI network analysis highlighted TP53 and EGFR as central hubs, validated using RNA-seq data. Co-expression and physical interaction analysis revealed a strong interplay between the common genes at the transcriptional and functional levels. …”
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Dairy Foods in a Moderate Energy Restricted Diet Do Not Enhance Central Fat, Weight, and Intra-Abdominal Adipose Tissue Losses nor Reduce Adipocyte Size or Inflammatory Markers in... by Marta D. Van Loan, Nancy L. Keim, Sean H. Adams, Elaine Souza, Leslie R. Woodhouse, Anthony Thomas, Megan Witbracht, Erik R. Gertz, Brian Piccolo, Andrew A. Bremer, Michael Spurlock

“…No diet differences were observed in weight, fat, or IAAT loss; nor SAT mRNA expression of inflammation, circulating cytokines, fasting lipids, glucose, or insulin. …”
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High-dose dual therapy for Helicobacter pylori eradication inducing less impact on the gut microbiota by Jia-Lun Guan, Ting-Ting Xu, Ya Lin, Yan-Shuai Mo, Bi-Yu He, Ying-Ying Han, Ji-Yan Li, Su-Hong Xia, Ya-Ni Zhou, Jia-Zhi Liao, Pei-Yuan Li

“…The diversity and composition of the gut microbiota were compared and analyzed in both groups using 16 S rRNA gene sequencing. Results Forty-nine H. pylori positive patients were enrolled and randomly assigned to either the HDDT (n = 24) or the BQT group (n = 25) group. …”
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Shaping the human gut microbiota: The role of canine companionship, lifestyle choices, and Blastocystis sp. by Zuzana Pavlíčková, Barbora Pafčo, Vladislav Ilík, Lee O'Brien Andersen, Milan Jirků, Kristýna Brožová, David Modrý, Oldřiška Kadlecová, Christen Rune Stensvold, Kateřina Jirků

“…Fecal DNAs from 118 humans and 54 dogs were subject to 16S rRNA gene sequencing using the Illumina MiSeq platform.Greater microbial diversity was observed in humans than in dogs. …”
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Genome-Wide Identification and In Silico Expression Analysis of <i>CCO</i> Gene Family in <i>Citrus clementina</i> (Citrus) in Response to Abiotic Stress by Sadaf Sarwar, Adnan Sami, Muhammad Zeshan Haider, Layba Tasawar, Jannat Akram, Arsalan Ahmad, Muhammad Shafiq, Haitham E. M. Zaki, Gabrijel Ondrasek, Muhammad Shafiq Shahid

“…To identify their structural features, they employed phylogenetic analysis, protein interactions, localization, structure, miRNA targets, evolutionary analysis, and transcriptome studies. …”
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The protoplast two-way fusions and fusant characteristics of Antrodia cinnamomea and Cordyceps militaris by Wei-Kuang Lai, Yang-Chang Wu, Tai-Sheng Yeh, Chun-Ren Hsieh, Yi-Hong Tsai, Chien-Kei Wei, Chi-Ying Li, Ying-Chen Lu, Fang-Rong Chang

“…We selected each of two best-growing fused colonies Ac-Cm-1, Ac-Cm-2, Cm-Ac-1, and Cm-Ac-2, together with parental A. cinnamomea and C. militaris, and studied their morphology, growth antagonism tests, and genetic relationships by 18S rRNA sequencing. In comparison with the initial cultures of 4 fusants, the yields of adenosine, biomass, cordycepic acid, cordycepin, total polysaccharide, and total triterpenoids were increased up 1.305−50.1563 times in the optimal medium conditions. …”
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Increased TLR4 Expression Aggravates Sepsis by Promoting IFN-γ Expression in CD38−/− Mice by Qi Li, Chenyi Wu, Zhenlong Liu, Huiqing Zhang, Yuna Du, Yuxiang Liu, Kuangyu Song, Qiaofa Shi, Rong Li

“…In addition, compared with CD38−/−TLR4mut mice, we found an increase of TLR4 expression and mRNA expression of these cytokines in the kidney of CD38−/− mice, although only increased IFN-γ level was detected in the serum. …”
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Metallothionein 1B attenuates inflammation and hepatic steatosis in MASH by inhibiting the AKT/PI3K pathway by Canghai Guan, Xinlei Zou, Wujiang Shi, Jianjun Gao, Chengru Yang, Yifei Ge, Zhaoqiang Xu, Shaowu Bi, Xiangyu Zhong

“…In vivo experiments demonstrate that specific downregulation of hepatic MT1B expression via AAV8-shMT1B injection significantly increases triglyceride and total cholesterol levels, exacerbates lipid accumulation, and markedly elevates liver fibrosis and inflammatory factor expression. RNA-seq and bioinformatics analyses show that the AKT/PI3K pathway is significantly suppressed in MT1B-overexpressing cells. …”
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hnRNPU-mediated pathogenic alternative splicing drives gastric cancer progression by Guoguo Jin, Yanming Song, Shaobo Fang, Mingyang Yan, Zhaojie Yang, Yang Shao, Kexin Zhao, Meng Liu, Zhenwei Wang, Zhiping Guo, Zigang Dong

“…This demethylation enhances the stability of hnRNPU mRNA, consequently promoting alternative splicing of MET. …”
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YTHDF2 in peritumoral hepatocytes mediates chemotherapy-induced antitumor immune responses through CX3CL1-mediated CD8+ T cell recruitment by Zhenyun Yang, Xin Wang, Yizhen Fu, Weijie Wu, Zili Hu, Qingyang Lin, Wei Peng, Yangxun Pan, Juncheng Wang, Jinbin Chen, Dandan Hu, Zhongguo Zhou, Li Xu, Yaojun Zhang, Jiajie Hou, Minshan Chen

“…Abstract Peritumoral hepatocytes are critical components of the liver cancer microenvironment, However, the role of peritumoral hepatocytes in the local tumor immune interface and the underlying molecular mechanisms have not been elucidated. YTHDF2, an RNA N 6 -methyladenosine (m6A) reader, is critical for liver tumor progression. …”
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