Inhibition of complement system-related gene ITGB2 attenuates epithelial–mesenchymal transition and inflammation in diabetic nephropathy by Jun Peng, Wenqi Zhao, Lu Zhou, Kun Ding

“…Also, VWF and ITGB2 were significantly related to the clinical features, including age, serum creatinine level, and GFR (MDRD). Besides, mRNA and protein expression levels of ITGB2 in HG-treated HK-2 cells were remarkably elevated. …”
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BDH1 identified by transcriptome has a negative effect on lipid metabolism in mammary epithelial cells of dairy goats by Mengke Ni, Saige Zang, Yihan Wang, Xiaochen Qin, Min Tian, Tiantian Xiong, Chong Chen, Yue Zhang, Jun Luo, Cong Li

“…In our previous study, BDH1 was identified as a key candidate gene regulating lipid metabolism in mammary glands of dairy goats by RNA-seq. This study aimed to investigate the effect of BDH1 on lipid metabolism in mammary epithelial cells of dairy goats (GMECs). …”
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Clinical and molecular response to alpha1‐oleate treatment in patients with bladder cancer by Farhan Haq, Samudra Sabari, Jaromir Háček, Antonín Brisuda, Ines Ambite, Michele Cavalera, Parisa Esmaeili, Murphy Lam Yim Wan, Shahram Ahmadi, Marek Babjuk, Catharina Svanborg

“…Uptake of alpha1‐oleate by the tumor triggered rapid shedding of tumor cells into the urine and cell death by an apoptosis‐like mechanism. RNA sequencing of tissue biopsies confirmed the activation of apoptotic cell death and strong inhibition of cancer gene networks, including bladder cancer related genes. …”
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Livogrit mitigates ANIT-induced cholestasis-like symptoms in an in vivo model by curbing hepatic inflammation and regulating BAX, TGF-β, MMP-9 and α-SMA gene expression by Acharya Balkrishna, Ritu Paliwal, Surjeet Singh, Rani Singh, Vivek Gohel, Rishabh Dev, Kunal Bhattacharya, Sandeep Sinha, Anurag Varshney

“…At molecular level, Livogrit inhibited up-regulation of BAX, TGF-β, α-SMA, and MMP-9 mRNA expressions, associated with the liver damages. …”
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Therapeutic potential of hydrogen-rich water in zebrafish model of Alzheimer’s disease: targeting oxidative stress, inflammation, and the gut-brain axis by Jiaxuan He, Peiye Xu, Ting Xu, Haiyang Yu, Lei Wang, Rongbing Chen, Kun Zhang, Yueliang Yao, Yanyan Xie, Qinsi Yang, Wei Wu, Da Sun, Dejun Wu

“…Behavioral assays, ELISA, Hematoxylin–eosin (H&E) staining, and reactive oxygen species (ROS) and neutrophil fluorescence labeling were employed to assess HRW’s impact. Additionally, 16S rRNA sequencing analyzed HRW’s effect on gut microbiota. …”
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Altered CD73-Adenosine Signaling Linked to Infection in Patients undergoing hemodialysis by Xiang F, Zhang Z, Nie Y, Cao X, Li Y, Chen X, Gong S, Zou J, Teng J, Ding X, Shen B

“…Animal models demonstrated reduced CD73 mRNA transcript and adenosine receptor levels, along with decreased serum adenosine levels in CKD. …”
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Analytic and clinical validity of thyroid nodule mutational profiling using droplet digital polymerase chain reaction by Vincent L. Biron, Ashlee Matkin, Morris Kostiuk, Jordana Williams, David W. Cote, Jeffrey Harris, Hadi Seikaly, Daniel A. O’Connell

“…Results A total of 208 patients underwent FNAB and mutational testing with the following Bethesda cytologic classification: 26.9% non-diagnostic, 55.2% benign, 5.3% FLUS/AUS, 2.9% FN/SPN, 2.4% SFM and 7.2% malignant. Adequate RNA was obtained from 91.3% (190) FNABs from which mutations were identified in 21.1% of HRAS, 11.5% of NRAS and 7.4% of BRAF. …”
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Nicotine-induced CHRNA5 activation modulates CES1 expression, impacting head and neck squamous cell carcinoma recurrence and metastasis via MEK/ERK pathway by Chen Feng, Wei Mao, Chenyang Yuan, Pin Dong, Yuying Liu

“…Knockdown of CHRNA5 can reduce cell migration, invasion, and proliferation, whereas nicotine exposure can reverse this trend. Additionally, the mRNA and protein expression of CES1 decreases with the knockdown of CHRNA5, indicating a regulatory relationship between the two. …”
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Analysis of the structure and function of the tomato <i>Solanum lycopersicum</i> L. MADS-box gene <i>SlMADS5</i> by A. V. Nezhdanova, M. A. Slugina, E. A. Dyachenko, A. M. Kamionskaya, E. Z. Kochieva, A. V. Shchennikova

“…Analysis of the expression of SlMADS5 and the tobacco genes NtLFY, NtAP1, NtWUS, NtAG, NtPLE, NtSEP1, NtSEP2, and NtSEP3 in leaves and apexes of transgenic and control plants showed that SlMADS5 mRNA is present only in tissues of transgenic lines. …”
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Effects of environmental bisphenol S exposure on male rat reproductive health and gut-blood-testicular axis integrity by Biran Zhu, Zequan Zhang, Ying Xie, Min Huang, Yu Chen, Yong Yang, Xiongjie Shi, Jian Han, Lihua Yang, Min Zhao

“…These observations were made by counting with a hemocytometer under the optical microscope. 16S rRNA and untargeted metabolomic elucidated potential impacts on the gut-blood-testicular axis: BPS impaired the physical barrier, evoked inflammation, and resulted in dysbiosis of the gut microbiota. …”
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Cooperative mechanisms of LexA and HtpG in the regulation of virulence gene expression in Pseudomonas plecoglossicida by Rongchao He, Yanfei Zuo, Qiu Li, Qingpi Yan, Lixing Huang

“…We employed an array of methods including molecular dynamics simulations, molecular docking, ChIP-seq, RNA-seq, mass spectrometry, gene mutagenesis, LacZ reporter assays, electrophoretic mobility shift assays, co-immunoprecipitation, and in vitro LexA degradation experiments. …”
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RoboGait: sistema robótico no invasivo para el análisis de la marcha humana by David Álvarez, Diego Guffanti, Alberto Brunete, Miguel Hernando, Ernesto Gambao

“…Para conseguirlo, se ha optado por mejorar la estimación de señales cinemáticas y espacio-temporales de la marcha procesando las medidas de la cámara con redes neuronales artificiales (RNA) entrenadas usando datos obtenidos de un sistema Vicon® certificado. …”
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Targeting EP2 Receptor Improves Muscle and Bone Health in Dystrophin^−/−/Utrophin^−/− Double-Knockout Mice by Xueqin Gao, Yan Cui, Greg Zhang, Joseph J. Ruzbarsky, Bing Wang, Jonathan E. Layne, Xiang Xiao, Johnny Huard

“…We found that Ep2, Ep4, Cox-2, 15-Pgdh mRNA, and PGE2 were significantly increased in DKO-Hom mice compared to wild-type (WT) mice. …”
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CDC20 and CCNB1 Overexpression as Prognostic Markers in Bladder Cancer by Hatice Sevim Nalkiran, Ilknur Biri, Ihsan Nalkiran, Hakki Uzun, Sumeyye Durur, Recep Bedir

“…This study aimed to investigate the gene expression profiles at different cancer stages to assess their potential prognostic value. <b>Methods:</b> RNA was extracted from paraffin-embedded BC tissues and the gene expression levels of CDC20 and CCNB1 were analyzed using qRT-PCR. …”
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بررسی بیان ژن‌های 1 HMGA- و 2 HMGA- در نمونه‌های تومور و طبیعی (حاشیه‌ی تومور) سرطان معده در خوزستان by محمدامین ندافی, آتوسا مرادزادگان

“…این مطالعه با هدف بررسی تغییرات بیان این پروتئین‌ها در جمعیت بیمار خوزستان، ایران انجام شد.روش‌ها: در این مطالعه، در مجموع 60 نمونه بافت شامل 30 نمونه‌ی تومور سرطان معده و 30 نمونه‌ی غیر توموری (حاشیه تومور) از بانک بافت تومور بیمارستان امام خمینی (ره) اهواز تهیه شد. پس از استخراج RNA و ارزیابی کمیت و کیفیت آن با استفاده از دستگاه نانودراپ و الکتروفورز ژل آگارز، ارزیابی بیان از بیان ژن‌های HMGA-1 و HMGA-2 با تکنیک Real-time PCR انجام شد. …”
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Decreased S100A9 Expression Promoted Rat Airway Smooth Muscle Cell Proliferation by Stimulating ROS Generation and Inhibiting p38 MAPK by Lei-Miao Yin, Xiao-Jie Han, Ting-Ting Duan, Yu-Dong Xu, Yu Wang, Luis Ulloa, Yong-Qing Yang

“…Rat ASMCs with stable S100A9 knockdown were generated using short hairpin RNA. The effects of decreased S100A9 expression on cellular proliferation, the production of reactive oxygen species (ROS), and p38 MAPK pathway protein expression were examined. …”
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Bacterial interactions underpin worsening lung function in cystic fibrosis-associated infections by Damian W. Rivett, Lauren R. Hatfield, Helen Gavillet, Michelle Hardman, Christopher van der Gast

“…Here, we suggest that looking at the interactions between species identified by 16S rRNA gene sequencing, rather than at species singularly, could elucidate hitherto unknown properties of these complicated infections. …”
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High Glucose and Lipopolysaccharide Prime NLRP3 Inflammasome via ROS/TXNIP Pathway in Mesangial Cells by Hong Feng, Junling Gu, Fang Gou, Wei Huang, Chenlin Gao, Guo Chen, Yang Long, Xueqin Zhou, Maojun Yang, Shuang Liu, Shishi Lü, Qiaoyan Luo, Yong Xu

“…We found that the expression of thioredoxin-interacting protein (TXNIP), NLRP3, and IL-1β was observed by immunohistochemistry in vivo. Simultaneously, the mRNA and protein levels of TXNIP, NLRP3, procaspase-1, and IL-1β were significantly induced by high glucose concentration and lipopolysaccharide in a dose-dependent and time-dependent manner in vitro. …”
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Development of a cell-penetrating peptide-based nanocomplex for long-term delivery of intact mitochondrial DNA into epithelial cells by Kyrie Wilson, Charles Holjencin, Hwaran Lee, Balasubramaniam Annamalai, Masaaki Ishii, Jeremy L. Gilbert, Andrew Jakymiw, Bärbel Rohrer

“…The generated nanoparticle complexes were found to be taken up by cells and localized to mitochondria, with exogenous mtDNA retention/maintenance, along with mitochondrial RNA and protein production, observed in mitochondria-depleted ARPE-19 cells at least 4 weeks following a single treatment. …”
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Synergistic interaction between wheat streak mosaic virus and Triticum mosaic virus modulates wheat transcriptome to favor disease severity by Haritha Nunna, Nathan A. Palmer, Gautam Sarath, Stephen N. Wegulo, Satyanarayana Tatineni, Satyanarayana Tatineni

“…Wheat transcriptome from singly (WSMV or TriMV) and doubly (WSMV+TriMV) infected upper uninoculated leaves were analyzed by RNA-Seq at 9, 12, and 21 days postinoculation. A total of 31,754 differentially expressed wheat genes were identified among all comparisons. …”
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