Effect of soluble dietary fiber extracted from Lentinula edodes (Berk.) Pegler on lipid metabolism and liver protection in mice on high-fat diet by Kangxiao Guo, Kangxiao Guo, Jing Liu, Zihan Yao, Zhoujin Tan, Tao Yang

“…After 4 weeks, we assessed general state, organ conditions, liver status, blood parameters, expression of hepatic lipid metabolism genes, mRNA levels of key hepatic lipid metabolism genes. …”
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Differentiation of Human Embryonic Stem Cells to Sympathetic Neurons: A Potential Model for Understanding Neuroblastoma Pathogenesis by Jane Carr-Wilkinson, Nilendran Prathalingam, Deepali Pal, Mohammad Moad, Natalie Lee, Aishwarya Sundaresh, Helen Forgham, Peter James, Mary Herbert, Majlinda Lako, Deborah A. Tweddle

“…After 7 days of PA6 cell coculture, mRNA expression of SNAIL and SOX-9 neural crest specifier genes and the neural marker peripherin (PRPH) increased. …”
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Characteristics and Differences in the Antler Velvet Microbiota During Regeneration by Yongxiang Li, Yuhang Zhu, Bo Yang, Shaochen Yu, Songze Li, André-Denis G. Wright, Rui Du, Huazhe Si, Zhipeng Li

“…In this study, we analyzed the antler velvet microbiota of sika deer at 15 days (Half) and 30 days (Full) post-pedicle casting using 16S rRNA gene sequencing. A total of 2659 OTUs were identified, which were assigned to 26 phyla, 304 families, and 684 genera. …”
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Cone photoreceptor phosphodiesterase PDE6H inhibition regulates cancer cell growth and metabolism, replicating the dark retina response by Ceren Yalaz, Esther Bridges, Nasullah K. Alham, Christos E. Zois, Jianzhou Chen, Karim Bensaad, Ana Miar, Elisabete Pires, Ruth J. Muschel, James S. O. McCullagh, Adrian L. Harris

“…The purpose of this study is to characterise the role of PDE6H in cancer cell growth. Methods From an siRNA screen for 487 genes involved in metabolism, PDE6H was identified as a controller of cell cycle progression in HCT116 cells. …”
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Bioinformatics insights into mitochondrial and immune gene regulation in Alzheimer's disease by Tian Meng, Yazhou Zhang, Yuan Ye, Hui Li, Yongsheng He

“…Moreover, qRT-PCR validation confirmed that the mRNA expression of the five biomarkers was consistent with bioinformatics results. …”
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Nicotinamide Mononucleotide Restores NAD⁺ Levels to Alleviate LPS-Induced Inflammation via the TLR4/NF-κB/MAPK Signaling Pathway in Mice Granulosa Cells by Mehboob Ahmed, Umair Riaz, Haimiao Lv, Muhammad Amjad, Sohail Ahmed, Shaokat Ali, Muhammad Usman Ghani, Guohua Hua, Liguo Yang

“…On the other hand, NMN supplementation restored the NAD⁺ levels and the NAD⁺/NADH ratio in GCs and significantly reduced the expression of pro-inflammatory markers at both mRNA and protein levels. It also enhanced cell viability and proliferation rates of GCs. …”
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Mixed radiation with different doses induces CCL17 to recruit CD8+T cell to exert anti-tumor effects in non-small cell lung cancer by Liuying Huang, Duo Wang, Muchen Xu, Danqi Qian, Yulin Cao, Xiaohan Wu, Liang Ming, Junhui Tang, Zhaohui Huang, Yuan Yin, Leyuan Zhou, Leyuan Zhou, Leyuan Zhou

“…This study sought to elucidate the influence of combining different doses of RT with immune checkpoint inhibitors (ICIs) on the infiltration of CD8+T cells within tumors, thereby augmenting the anti-tumor response.MethodsConstructing a mouse model featuring bilateral lung cancer tumors subjected to high and low dose irradiation, the analysis of RNA transcriptome sequencing data and immunohistochemical validation for tumors exposed to various dosages guided the selection of the optimal low-dose irradiation scheme. …”
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启动子DNA甲基化和组蛋白乙酰化对胃癌中PDX1 表达的影响

“…5’-aza-dC和TSA处理胃癌细胞,RT-PCR检测PDX1 mRNA水平,分析DNA去甲基化和组蛋白乙酰化对PDX1表达的影响;构建6个PDX1启动子片段至pGL3载体,检测转录活性,分析去甲基化和乙酰化对PDX1启动子转录活性的影响?…”
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Evaluation of X-Inactivation Status and Cytogenetic Stability of Human Dermal Fibroblasts after Long-Term Culture by Zhi-Gang Xue, Zhan-Ping Shi, Juan Dong, Ting-Ting Liao, Yan-Peng Wang, Xue-Ping Sun, Zheng-Jie Yan, Xiao-Qiao Qian, Yu-Gui Cui, Juan Chen, Jia-Yin Liu, Guoping Fan

“…However, four out of the eight HDF lines at high passage numbers (≥P10) exhibited duplicated hallmarks of inactive X chromosome including two punctuate signals of histone H3 lysine 27 trimethylation (H3K27me3) and X inactive-specific transcript (XIST) RNA signals in approximately 8.5–18.5% of the cells. …”
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Prognostic Impact of miR-224 and RAS Mutations in Medullary Thyroid Carcinoma by Elisabetta Cavedon, Susi Barollo, Loris Bertazza, Gianmaria Pennelli, Francesca Galuppini, Sara Watutantrige-Fernando, Simona Censi, Maurizio Iacobone, Clara Benna, Federica Vianello, Stefania Zovato, Davide Nacamulli, Caterina Mian

“…Little is known about the function of microRNA-224 (miR-224) in medullary thyroid cancer (MTC). …”
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The Mycoplasma hyopneumoniae protein Mhp274 elicits mucosal and systemic immune responses in mice by Mengqi Xie, Zhongshun Huang, Yun Zhang, Yujie Gan, Huiying Li, Dan Li, Honglei Ding

“…The levels of IFN-γ, IL-4, and IL-17, in the supernatants of splenocytes and in serum were measured, and the mRNA levels of three cytokines in splenocytes were analyzed. …”
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Integrative analysis of metabolite and transcriptome reveals the biosynthetic pathway and candidate genes for iridoid glycoside biosynthesis in Neopicrorhiza scrophulariiflora (Pen... by Ke Rao, Ke Rao, Siyu Liu, Siyu Liu, Xiaohui Tang, Guofu Jia, Shaohua Yang, Chaoxiang Ren, Chaoxiang Ren, Jin Pei, Jin Pei

“…In this study, widely targeted metabolomics and RNA-seq technology were employed to perform a joint analysis of the metabolome and transcriptome in different tissues of N. scrophulariiflora, including the roots, stems, and leaves. …”
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Artificial neural network systems to predict the response to sintilimab in squamous-cell non-small-cell lung cancer based on data of ORIENT-3 study by Tongji Xie, Guangyu Fan, Le Tang, Puyuan Xing, Yuankai Shi

“…Methods Four ANN systems based on bulk RNA data to predict disease control (DC), immune DC (iDC), objective response (OR) and immune OR (iOR) were constructed and tested for patients with sqNSCLC treated with sintilimab. …”
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Nephropathy in Hypertensive Animals Is Linked to M2 Macrophages and Increased Expression of the YM1/Chi3l3 Protein by Paula Andréa Malveira Cavalcante, Natalia Alenina, Alexandre Budu, Leandro Ceotto Freitas-Lima, Thaís Alves-Silva, Juan Sebastian Henao Agudelo, Fatimunnisa Qadri, Niels Olsen Saraiva Camara, Michael Bader, Ronaldo Carvalho Araújo

“…We examined the kidneys of TGM123-FVB/N animals to assess macrophage polarization and end-organ damage. mRNA expression was evaluated using real-time PCR, and protein levels were assessed through ELISA, CBA, Western blot, and immunofluorescence. …”
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Preparation of pH-Responsive Tanshinone IIA-Loaded Calcium Alginate Nanoparticles and Their Anticancer Mechanisms by Tianying Ren, Jing Wang, Yingxin Ma, Yichen Huang, Somy Yoon, Lijun Mu, Ru Li, Xuekun Wang, Lina Zhang, Pan Li, Lusha Ji

“…The physicochemical properties of Tan IIA/CA nanoparticles were evaluated, including their size, stability, and release profile. We also utilized RNA sequencing to further investigate the underlying anticancer mechanisms of Tan IIA/CA nanoparticles. …”
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Microbial keratitis in Southern Malawi: a microbiological pilot study by Timothy Neal, Dean Everett, Stephen B Kaye, Alistair Darby, Moira Gandiwa, Shaffi Mdala, Caroline E Corless, Rose Herbert, Nicholas A V Beare, Tobi F Somerville, Thokozani Zungu, Malcolm J Horsburgh, Petros C Kayange

“…Samples were processed using culture and PCR for Acanthamoeba, herpes simplex virus type 1 (HSV-1) and the bacterial 16S rRNA gene. Minimum inhibitory concentrations of isolates to eight antimicrobials were measured using susceptibility strips. …”
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Evaluation of Theileria equi vertical transmission rate and routes in a cohort of asymptomatic mares and their foals by Lisa-Marie Hermans, Claire Bonsergent, Anne Josson, Gloria Rocafort-Ferrer, Marine Le Guyader, Sophie Angelloz-Pessey, Agnès Leblond, Laurence Malandrin

“…One foal was born carrier but T. equi DNA had not been detected in the placenta. The 18S rRNA genotype E of T. equi was characterized in the four foals, foetal parts of the placenta and colostrum. …”
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Occurrence of macrolides resistance in Legionella pneumophila ST188: Results of the Belgian epidemiology and resistome investigation of clinical isolates by Charlotte Michel, Fedoua Echahidi, Geraldine De Muylder, Max Sewell, Ian Boostrom, Olivier Denis, Owen B. Spiller, Denis Pierard

“…Genotypic screening identified a 23S ribosomal RNA mutation linked to a high-level macrolide resistance in one isolate of ST188, which is genetically closed to resistant isolates from France. …”
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Soy polysaccharide maintains colonic homeostasis to protect from dextran sulphate sodium-induced colitis by modulating gut microbiota and intestinal epithelial regeneration by Nana Zhang, Jianlin Liu, Weiyue Zhang, Xinxin Guo, Shuying Li, Hongtai Zhang, Minjie Wang, Bei Fan, Fengzhong Wang

“…In addition, bacterial 16S rRNA sequencing analysis showed that SP modulated the composition of fecal microbiota, including selectively increasing Lactobacillus relative abundance. …”
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Human blood cell transcriptomics unveils dynamic systemic immune modulation along colorectal cancer progression by Pedro J Romero, Mauro Delorenzi, Amaia Martínez-Usatorre, Laura Ciarloni, Noushin Hadadi, Eric Durandau, Sylvain Monnier-Benoit, Paolo Angelino, Victoria Wosika, Alessandra Jordano Conforte, Sara S Fonseca Costa, Hector Fabio Satizabal, Jérémie Despraz, Andres Perez-Uribe, Stephan Morgenthaler, Brian Hashemi, Sahar Hosseinian-Ehrensberger

“…Here we propose the host immune response to the onset of cancer as an alternative approach for early detection of CRC.Methods We perform whole transcriptome analysis of peripheral blood mononuclear cells (PBMCs) isolated from individuals with CRC, precancerous lesions or negative colonoscopy in two independent cohorts using next-generation sequencing.Results We discover and validate novel early CRC RNA biomarkers. Taking into account, and adjusting for, the sensitivity of PBMCs transcriptomes to processing times, we report distinct transcriptomic changes in the periphery related to specific CRC stages. …”
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