PLIN5 contributes to lipophagy of hepatic stellate cells induced by inorganic arsenic by Haomiao Li, Xuri Xie, Tianming Qiu, Jingyuan Zhang, Jie Bai, Guang Yang, Ningning Wang, Xiaofeng Yao, Xiance Sun

“…Remarkably, arsenic exposure increased the expression levels of Perilipin 5 (PLIN5), a lipid droplet-associated protein, both at the mRNA and protein levels. Moreover, silencing PLIN5 influenced arsenic-induced lipolysis. …”
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Knockdown of NDUFAF6 inhibits breast cancer progression via promoting mitophagy and apoptosis by Shang Wu, Xindi Ma, Xiangmei Zhang, Kaiye Du, Chao Shi, Ahmed Ali Almaamari, Boye Han, Suwen Su, Yunjiang Liu

“…We knocked down NDUFAF6 in breast cancer cells using small interfering RNA and investigated its effects on cell proliferation and migration ability. …”
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Calcitonin Gene-Related Peptide (Cgrp), Adrenomedullin (Am), Amylin, And Calcitonin (Ct) Receptors And Overlapping Biological Actions by J.A. Fischer, W. Born, R. Muff

“…Another CGRP receptor isotype remains to be discovered in the cerebellum with no detectable CRLR encoding mRNA. Overlapping biological actions include inhibition of bone resorption obtained predominantly with CT, but also at high concentrations with CGRP, AM, and amylin. …”
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Enhanced membrane protein production in HEK293T cells via ATF4 gene knockout: A CRISPR-Cas9 mediated approach by Byung-Jo Choi, Ba Reum Kim, Ho Joong Choi, Ok-Hee Kim, Say-June Kim

“…The ATF4 gene was edited by infecting HEK293T cells with a lentivirus carrying optimized single-guide RNA (ATF4-KO-3) and Cas9 genes. Comparative evaluations were conducted using all-in-one and two-vector systems. …”
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Mechanism of Action of Decitabine in the Treatment of Acute Myeloid Leukemia by Regulating LINC00599 by Fan Du, Ting Jin, Li Wang

“…Reverse transcription polymerase chain reaction (RT-PCR) was performed to examine the expression of lncRNA LINC00599. The expression of apoptosis-related proteins was analyzed using western blotting. …”
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BRCA1 is involved in sustaining rapid antler growth possibly via balancing of the p53/endoplasmic reticulum stress signaling pathway by Qianqian Guo, Zhen Wang, Jiping Li, Chao Ma, Junjun Zheng, Hengxing Ba, Guokun Zhang, Chunyi Li

“…Lack of BRCA1 promoted cell apoptosis and cell senescence and inhibited cell proliferation and cell self-renewal. RNA-seq results showed that in the absence of BRCA1, the p53 signaling pathway was significantly up-regulated. …”
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Antimicrobial Susceptibility of <i>Glaesserella parasuis</i> to Macrolides and Characterization of <i>erm</i>(T)-Carrying Mobile Elements on Chromosome by Peng Zhang, Changmin Li, Shuna Shang, Ting Huang, Junqi Liu, Qianwen Ge, Xiaoping Liao, Liangxing Fang, Yang Yu

“…The macrolide resistance gene <i>erm</i>(T) and the A2059G mutation in 23S rRNA were detected in the current study. To the best of our knowledge, integrative and conjugative element (ICE)-borne <i>erm</i>(T) in <i>G. parasuis</i> is reported for the first time in this study. …”
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Adjunctive diagnostic tool for histopathological classification of congenital mesoblastic nephroma based in molecular genetic findings by Hiroshi Hamada, Kenichi Kohashi, Takeshi Iwasaki, Mikiko Hashisako, Yuko Hino, Masahiro Fukuhara, Amane Kamouchi, Naonori Kawakubo, Tatsuro Tajiri, Yoshinao Oda

“…Reverse transcription-polymerase chain reaction was performed for the NTRK gene fusion and EGFR-ITDs in all cases. Comprehensive mRNA analysis was performed using the nCounter® Gene Expression Assay. …”
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Anti-colorectal cancer effect of total minor ginsenosides produced by lactobacilli transformation of major ginsenosides by inducing apoptosis and regulating gut microbiota by Yunjiao Shen, Yunjiao Shen, Yansong Gao, Ge Yang, Zijian Zhao, Yujuan Zhao, Lei Gao, Shengyu Li

“…The level of apoptosis and expression of various proteins in the tumor tissues were detected by immunohistochemistry and Western blot. High-throughput 16S rRNA sequencing was used to determine the alterations in the gut microbiota.ResultsIn vitro studies demonstrated that TMG significantly inhibited proliferation and promoted apoptosis in CT26.WT cells. …”
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ＥＮＨ 蛋白与病理性疼痛相关性研究

“…ＥＮＨ与正常对照组相比，ＳＮＩ组术后手术侧痛敏、ＥＮＨ蛋白及ｍＲＮＡ有一过性增高继而恢复正常，并随时间推移而逐渐再次增高（ｎ＝ ５／组）。【结论】 ＥＮＨ蛋白在大鼠慢性病理性疼痛模型中有表达，且其改变与痛敏的进程一致。…”
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Improving polyketide biosynthesis by rescuing the translation of truncated mRNAs into functional polyketide synthase subunits by Yan Liu, Chaoyi Song, Qingwen Cui, Hongluan Sun, Chanjuan Jiang, Ruofei Guo, Ruoting He, Zhen Li, Ji Luan, Hailong Wang

“…Splitting the large busA gene rescues translation of truncated mRNAs into functional PKS subunits, and increases the biosynthetic efficiency of butenyl-spinosyn PKS by 13-fold. The truncated mRNA translation rescue strategy will facilitate engineering of multi-domain proteins to enhance their functions.…”
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Long-term effect of repeated application of pig slurry digestate on microbial communities in arable soils by Daniela Mora-Salguero, Lionel Ranjard, Thierry Morvan, Samuel Dequiedt, Vincent Jean-Baptiste, Sophie Sadet-Bourgeteau

“…Changes in soil microbial communities were analyzed through molecular microbial biomass and diversity assessments using high-throughput sequencing targeting 16S and 18S ribosomal RNA genes. DNA extraction and molecular analyses were performed on soil samples collected at the start of the trial in 2012 and subsequently in 2017 and 2022. …”
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Sleep-Aiding Effect of Casein-Derived Peptide TPTLN and Its Underlying Mechanism by DENG Shijie, LIU Ling, HUANG Ju, FENG Fengqin, ZHAO Minjie, DU Juan

“…Enzyme-linked immunosorbent assay (ELISA) was used to determine the levels of 5-hydroxytryptamine (5-HT), γ-aminobutyric acid (GABA), 5-hydroxyindoleacetic acid (5-HIAA), glutamate (Glu), β-endorphin (β-EP), cortisol, norepinephrine (NE) in the serum and brain tissue, and the GABA/Glu ratio was evaluated. real-time quantitative polymerase chain reaction (qPCR) was used to detect the effect of TPTLN on the mRNA relative expression levels of γ-aminobutyric acid A receptor α1 (GABAA-α1) and 5-hydroxytryptamine receptor 1A (5-HT1A) in the hypothalamus. …”
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A New Prognostic Risk Model Based on PPAR Pathway-Related Genes in Kidney Renal Clear Cell Carcinoma by Yingkun Xu, Xiunan Li, Yuqing Han, Zilong Wang, Chenglin Han, Ningke Ruan, Jianyi Li, Xiao Yu, Qinghua Xia, Guangzhen Wu

“…For this study, we first found the PPAR pathway-related genes on the gene set enrichment analysis (GSEA) website and found the KIRC mRNA expression data and clinical data through TCGA database. …”
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Genetically edited human placental organoids cast new light on the role of ACE2 by Anya L. Arthurs, Bianca Dietrich, Martin Knöfler, Caleb J. Lushington, Paul Q. Thomas, Fatwa Adikusuma, Jessica M. Williamson, Susan Babikha, Tyla Damhuis, Tanja Jankovic-Karasoulos, Melanie D. Smith, Kirsty G. Pringle, Claire T. Roberts

“…Placental organoids with the ACE2 rs2074192 TT genotype had significantly higher expression of ACE2 mRNA and ACE2 protein with elevated ACE2:ACE expression ratio and no change in ACE protein. …”
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Phthalates toxicity in vivo to rats, mice, birds, and fish: A thematic scoping review by Mohammad Shah Alam, Zannatul Maowa, Mohammad Nazmol Hasan

“…The most commonly used techniques were H&E, RT-qPCR, ROS assay, WB, IHC, ELISA, RIA, TUNEL, TEM, IFM, FCM, and RNA-seq. Conclusions: DEHP and DBP are the most toxic and studied phthalates, while BBP, DiNP, DiBP, DiDP, BBOP, DMP, and DiOP and their combinations require more accurate studies to confirm their toxic effects on human health and mechanisms of action. …”
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Exploring the influence of pre-analytical variables on gene expression measurements and relative expression orderings in cancer research by Tian Tian, Guie Lai, Ming He, Xiaofang Liu, Yun Luo, You Guo, Guini Hong, Hongdong Li, Kai Song, Hao Cai

“…These paired samples were utilized to assess the impact of pre-analytical variables on gene expression measurements and REOs, including sampling methods, tumor sample heterogeneity, fixed time delays, preservation conditions, degradation levels, library preparation kits, amplification kits, RNA quantity, measuring platforms, and laboratory sites at single and multi-variable level. …”
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Chromosome-level reference genome assembly for the mountain hare (Lepus timidus) by Fekete, Zsófia, Absolon, Dominic E., Michell, Craig, Wood, Jonathan M. D., Goffart, Steffi, Pohjoismäki, Jaakko L. O.

“…We present here a high-quality genome assembly of a male mountain hare (Lepus timidus Linnaeus), from Ilomantsi, Eastern Finland, utilizing an isolated fibroblast cell line as the source for high quality DNA and RNA. Following the previously published brown hare reference genome assembly, the mountain hare is the second Finnish pilot species for the European Reference Genome Atlas (ERGA) initiative, a collaborative effort to generate reference genomes for European biodiversity. …”
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MnGA with multiple enzyme-like properties for acute wound healing by reducing oxidative stress and modulating signaling pathways by Xueting Guo, Wenqi Wang, Liting Lin, Jie Shan, Junyao Zhu, Shipeng Ning, Hanmei Li, Xianwen Wang, Decheng Lu

“…Moreover, the scavenging of RNS by MnGA can amplify the anti-inflammatory effect in combination with the scavenging of ROS. RNA sequencing of mouse skin tissue further revealed that MnGA significantly reduces inflammation by modulating the nuclear factor kappa-B (NF-κB), Toll-like receptor (TLR), and NOD-like receptor (NLR) signaling pathways and promotes skin regeneration. …”
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Splicing and expression dynamics of SR genes in hot pepper (Capsicum annuum): regulatory diversity and conservation under stress by Lin Li, Yueqin Zhang, Rui Zhang, Rui Zhang, Xiangtao Cen, Yongxiang Huang, Yongxiang Huang, Hanqiao Hu, Hanqiao Hu, Xingyu Jiang, Xingyu Jiang, Yu Ling, Yu Ling

“…Interestingly, a cross-species comparative study with Arabidopsis (Arabidopsis thaliana) and tomato (Solanum lycopersicum) showed that many AS events impact the region which codes the RNA recognition motif (RRM) domain of the protein, indicating a conserved regulatory mechanism of SR proteins in plants. …”
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