Plasma galectin-3 is associated with decreased glomerular filtration rate in chronic HIV by Diana L. Vares-Lum, Louie Mar A. Gangcuangco, Juwon Park, Eduardo Manzano, Michael Ortega, Dominic C. Chow, Cecilia Shikuma

“…Among PLWH, majority had undetectable plasma HIV RNA (82.2%). Gal-3 was significantly higher in PLWH than in HIV-uninfected participants (6.4 [IQR 4.0, 8.5] ng/mL and 4.5 [IQR 2.3, 6.5] ng/mL, respectively; p = 0.020) while a trend towards lower eGFR was found in PLWH compared to the HIV-uninfected cohort (86.8 [IQR 71.3, 91.8] and 89.0 [IQR 78.6, 97.4] mL/min/1.73 m2, respectively; p = 0.071). …”
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Foodborne toxin Aflatoxin B1 induced glomerular podocyte inflammation through proteolysis of RelA, downregulation of miR-9 and CXCR4/TXNIP/NLRP3 pathway by Jie Zhang, Shuang Yang, Baocai Xu, Zihui Qin, Xinyi Guo, Ben Wei, Qinghua Wu, Kamil Kuca, Tushuai Li, Wenda Wu

“…Reduction of RelA resulted in decreasing microRNA-9 (miR-9) and activating the chemokine receptor 4 (CXCR4), thioredoxin interacting protein (TXNIP), and NOD-like receptor pyrin domain-containing 3 (NLRP3) signaling axis (CXCR4/TXNIP/NLRP3 pathway), leading to podocyte inflammation. …”
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The first two mitochondrial genomes for the genus Ramaria reveal mitochondrial genome evolution of Ramaria and phylogeny of Basidiomycota by Qiang Li, Lijiao Li, Ting Zhang, Peng Xiang, Qian Wu, Wenying Tu, Zhijie Bao, Liang Zou, Cheng Chen

“…We detected large-scale gene rearrangements between Phallomycetidae mitogenomes, including gene displacement and tRNA doubling. A total of 4499 bp and 7746 bp aligned fragments were detected between the mitochondrial and nuclear genomes of R. cfr. rubripermanens and R. rubella, respectively, indicating possible gene transferring events. …”
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Evaluation of chicken liver meal as fish meal alternative in large yellow croaker Larimichthys crocea diet: Effects of growth performance, antioxidant capacity, intestinal health a... by Jianchun Shao, Yujie Wang, Liwen Wu, Qingyin Zheng, Wenbo Zhu, Zhengbang Chen, Dianguang Zhang

“…The CLM75 and CLM100 diets led to a significant decrease in the activity of amylase (AMS), lipase (LPS), catalase (CAT) as well as total antioxidant capacity (T-AOC) for liver of Larimichthys crocea, while remarkably increasing malondialdehyde (MDA) content. 16S rRNA sequencing revealed that CLM75 and CLM100 diets resulted in increased relative abundances of Proteobacteria phylum and decreased relative abundances of Firmicutes phylum. …”
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The extensive cultivation of Pyropia haitanensis along the coastal areas influences the ecological dynamics of nearby intertidal mudflat pond water by Peihao Liu, Qijun Luo, Shanshan Zhu, Juanjuan Chen, Rui Yang, Tingting Niu, Tiegan Wang, Peng Zhang, Haimin Chen

“…The dynamic diversity patterns and structure of microbial communities were determined by 16S and 18S rRNA sequencing, and the environmental factors were measured.ResultsIn the offshore aquaculture area, the cultivation of P. haitanensis increased the dissolved oxygen and pH, decreased inorganic nutrients, reduced the alpha diversity of bacteria, established the dominance of Proteobacteria, but did not influence the composition of microeukaryotes. …”
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Leptospirosis Risk Assessment in Rodent Populations and Environmental Reservoirs in Humanitarian Aid Settings in Thailand by Panadda Krairojananan, Kasima Wasuworawong, Surachai Leepitakrat, Taweesak Monkanna, Elizabeth W. Wanja, Silas A. Davidson, Betty K. Poole-Smith, Patrick W. McCardle, Alyssa Mann, Erica J. Lindroth

“…A surveillance program was conducted at Engineering Civil Assistance Program (ENCAP) training sites using real-time PCR, dark-field microscopy, and 16S rRNA gene sequencing to detect <i>Leptospira</i> in rodents and environmental samples. …”
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Genetic characterization of clinical <I>Klebsiella</I> isolates circulating in Novosibirsk by A. V. Bardasheva, N. V. Fomenko, T. V. Kalymbetova, I. V. Babkin, S. O. Chretien, E. V. Zhirakovskaya, N. V. Tikunova, V. V. Morozova

“…Species identification of strains was performed using 16S rRNA and rpoB gene sequences. It was revealed that Klebsiella pneumoniae strains were dominant in the population (57 strains), while the remaining 15 strains were K. grimontii, K. aerogenes, K. oxytoca and K. quasipneumoniae. …”
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DNMT1-driven methylation of RORA facilitates esophageal squamous cell carcinoma progression under hypoxia through SLC2A3 by Wenjian Yao, Linlin Shang, Yinghao Wang, Lei Xu, Yu Bai, Mingyu Feng, Xiangbo Jia, Sen Wu

“…The promoter methylation level of RORA was detected by DNA pyrosequencing. RNA-seq analysis was performed to explore the downstream mechanisms of RORA, and the transcriptional regulation of RORA on SLC2A3 was verified by ChIP-qPCR and dual-luciferase reporter assay. …”
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Collection of microorganisms of ICG SB RAS as a genetic resource for biotechnology by A. V. Bryanskaya, Y. E. Uvarova, A. S. Rozanov, N. M. Slynko, V. N. Shlyakhtun, K. V. Starostin, E. A. Demidov, E. V. Lazareva, O. P. Taran, S. E. Peltek

“…DNA isolation and the sequencing of 16S rRNA gene sequences were performed. The strains of the collection were characterized by morphological, physiological, moleculargenetic and mass-spectrometric characteristics. …”
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LIPUS promotes osteogenic differentiation of rat BMSCs and osseointegration of dental implants by regulating ITGA11 and focal adhesion pathway by Chao Liang, Yuqing Zhang, Yuwei Yan, Wei Geng, Jun Li, Xiu Liu

“…Bioinformatics analysis of RNA-seq data revealed that the upregulated genes in BMSCs after LIPUS treatment were significantly enriched in osteoblast differentiation-related functions and focal adhesion-related pathways. …”
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Ficus deltoidea standardized methanolic extract improves kidney structure and function against streptozotocin-induced diabetic nephropathy in rats by Nurdiana Samsulrizal, Noor Syaffinaz Noor Mohamad Zin, Nur Syimal`ain Azmi, Mohd Daud Bakar, Hafandi Ahmad, Yong-Meng Goh

“…We noticed that biochemical and pathological changes corresponded to the infrared (IR) peaks at 1545 cm-1 and 1511 cm-1 (amide II), 1246 cm-1 (DNA/RNA phosphate backbones), and 1181 cm-1 (C–O vibrations). …”
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Clinicopathological Analysis of 14 Cases of Primary Pulmonary Lymphoepithelial Carcinoma by Yixuan FANG, Anzhe WANG, Lumin SHEN, Xiao YUAN, Yu KONG

“…Epstein-Barr virus-encoded RNA (EBER) in situ hybridization was positive in all instances. …”
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Undernutrition disrupts jejunal and ileal microbiota and epithelial tissue homeostasis in a pregnant sheep model by Weibin Wu, Muhammad Faheem Akhtar, Jiahong Geng, Huizhen Lu, Muhammad Ajwad Rahim, Jianbo Cheng, Xiaoling Ding, Shengyong Mao, Yanfeng Xue

“…After 15-d treatment, all ewes were slaughtered, and jejunal and ileal digesta and epithelium samples were collected for 16S rRNA gene sequencing and transcriptome sequencing, respectively. …”
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Thermosensitive injectable in situ forming hydrogel incorporating anti-oxidative stress and anti-inflammatory nanoparticles for vital pulp therapy of pulpitis by Lixin Zhang, Xianling Ning, Jingyi Cui, Yanqiang Zhao, Fei Xie, Ningxin Zhu, Dan Wang, Man Qin, Zhiqiang Lin, Yuanyuan Wang

“…As a direct pulp capping material, PBNPs@PPP suppressed inflammation progression in pulpitis in rats while also reducing cell necrosis and inflammatory cell infiltration in pulp tissue. RNA sequencing analyses showed that the functions of PBNPs@PPP were highly involved in anti-inflammatory pathways. …”
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RIPK2-Mediated Autophagy and Negatively Regulated ROS-NLRP3 Inflammasome Signaling in GMCs Stimulated with High Glucose by Chenlin Gao, Jiao Chen, Fang Fan, Yang Long, Shi Tang, Chunxia Jiang, Jiying Wang, Youhua Xu, Yong Xu

“…GMCs were divided into the following groups: normal group (NC), high glucose group (HG), and RIPK2 siRNA group. RIPK2, LC3, caspase1, and IL-1β levels were measured by western blotting and RT-PCR. …”
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Survey of the Applications of NGS to Whole-Genome Sequencing and Expression Profiling by Jong-Sung Lim, Beom-Soon Choi, Jeong-Soo Lee, Chanseok Shin, Tae-Jin Yang, Jae-Sung Rhee, Jae-Seong Lee, Ik-Young Choi

“…Whole-genome expression profile data are useful to approach genome system biology with quantification of expressed RNAs from a whole-genome transcriptome, depending on the tissue samples. The hybrid mRNA sequences from Rohce/454 and Illumina/Solexa are more powerful to find novel genes through de novo assembly in any whole-genome sequenced species. …”
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Two new strains of Streptomyces with metabolic potential for biological control of pear black spot disease by Yi-Huang Chen, Jia-Xing Zhang, Yan Min, Yang Liu, Jian-Ming Wang, Lin-Quan Bai, Xiao-Xia Luo

“…Methods In this study, the genetic background of the strain was elucidated through 16 S rRNA gene analysis and multiphase taxonomic identification methods. …”
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Development and Characterization of Anti-Nitr9 Antibodies by Radhika N. Shah, Ivan Rodriguez-Nunez, Donna D. Eason, Robert N. Haire, Julien Y. Bertrand, Valērie Wittamer, David Traver, Shila K. Nordone, Gary W. Litman, Jeffrey A. Yoder

“…Within the zebrafish NITR family, nitr9 is the only gene predicted to encode an activating receptor. However, alternative RNA splicing generates three distinct nitr9 transcripts, each of which encodes a different isoform. …”
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ERK1/2 Inhibition Alleviates Diabetic Cardiomyopathy by Suppressing Fatty Acid Metabolism by Erin McLean, Caroline De Roo, Annabel Maag, Megan Coble, Jefferson Cano, Ruijie Liu

“…Real-time polymerase chain reaction (PCR) analysis of the messenger ribonucleic acid (mRNA) expression was performed for hypertrophy (ANF, BNP, and βMHC), fibrosis (Col3α1), and fatty acid metabolism genes (PPARα, CPT1A, and FACS). …”
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Silencing circ_0043256 inhibited CoCl2-induced proliferation, migration, and aerobic glycolysis in gastric cancer cells by Wenting Ou, Rongjian Tan, Jiawei Zhai, Lijun Sun, Zhenhao Quan, Xianjin Huang, Feipeng Xu, Qingwen Xu, Caijin Zhou

“…Abstract We aimed to explore the role of circular RNA 0043256 (circ_0043256) in gastric cancer (GC) and its underlying mechanisms. …”
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